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번데기동충하초(Cordyceps militaris) 추출물의 세포독성 및 유전독성 억제효과
김미남(Mi-Nam Kim),최승필(Cheng-Bi Cui),이득식(Deuk-Sik Lee),함승시(Seong-Shi Ham) 한국식품영양과학회 2001 한국식품영양과학회지 Vol.30 No.5
각종 암세포에 대한 번데기동충하초(Cordyceps militaris) 추출물 및 분획물의 세포독성을 규명하기 위하여 암세포로 A549, MCF-7, HeLa, HT1080, Hep3B, KATOⅢ 및 K562를 이용하였다. 그 결과, SRB assay에 의한 1 mg/mL의 에탄올 추출물 농도에서 HT1080, HeLa, Hep3B 그리고 A549는 각각 89.4%, 85.7%, 72.9% 그리고 65.5%의 억제효과를 나타내었다. MCF7, HeLa 그리고 HT1080 세포의 경우는 핵산 분획물 1 mg/mL 농도에서 각각 92.9%, 90.3% 그리고 97.0%로 다른 분획물보다 현저히 높은 억제효과를 나타냈다. KATOⅢ 세포에 대한 억제효과는 1 mg/mL 투여시 에탄올 추출물의 경우 61.5%를 보였고 부탄올과 물 분획물에서 각각 83.7%와 80.4%로 다소 높은 억제효과를 보였다. K562 세포에 대한 시료의 억제효과는 에탄올 추출물(1 mg/mL)이 60.5%의 억제율을 보인데 반해 같은 농도에서 에틸 아세테이트, 부탄올 및 핵산 분획물에서는 각각 88.6%, 80.6% 및 75.6%로 높은 억제효과를 보였다. 번데기동충하초 에탄올 추출물의 소핵생성 억제효과는 양성대조군의 11.2±0.8에 비하여 10, 20, 40, 80 mg/kg의 농도에서 10.5±0.8, 8.3±0.5, 7.8±0.3, 3.7±0.6로 8.7, 25.9, 30.4 그리고 67.0%의 유의적인 억제효과를 나타내었다. 또한 각각의 분획물에 대한 소핵생성 억제효과는 유전손상물질(MNNG: 150 mg/kg, I.P.)만 투여한 양성대조군에 비해서 시료 농도를 10, 20, 40, 80 mg/kg 처리하였을 경우 동일한 시료 농도 80 mg/kg에서 부탄올, 에틸 아세테이트, 물 그리고 클로로포름 분획물이 각각 3.1±0.3, 3.7±0.6, 4.2±0.3, 4.8±0.3으로 72.3%, 67.0%, 63.0% 그리고 57.1%의 순으로 소핵생성 억제효과를 나타내었으며, 그 중 핵산 분획물이 2.8±0.5의 75.0%로 가장 높은 소핵생성 억제효과를 나타내었다. This study was performed to investigate the effects on the cytotoxicity and antigenotoxicity of Cordyceps militaris extracts on the human cancer cell lines. The ethanol extract and five fractions which were hexane, chloroform, ethylacetate, butanol and aqueous were screened for cytotoxicity on human lung carcinoma (A549), human breast adenocarcinoma (MCF-7), human epitheloid carcinoma (HeLa), human fibrosarcoma (HT1080), human hepatocellular carcinoma (Hep3B), human gastric carcinoma (KATOⅢ) and chronic myelogenous leukemia (K562) cells by SRB and MTT assays. The results showed that growth inhibition rates of the human cancer cells in the presence of Cordyceps militaris were inhibited with increasing concentration of the extract. The ethanol extract from Cordyceps militaris had strong inhibitory effects in 1 mg/mL treatment by SRB assay, showing 89.4%, 85.7%, 72.9% and 65.5% inhibition in HT1080, HeLa, Hep3B and A549, respectively. The treatment of 1 mg/mL hexane fraction by SRB assay had the strongest cytotoxicity with 97.0% on HT1080 followed by MCF-7 (92.9%) and HeLa (90.3%). The inhibition ratio on KATOIII by MTT assay was much higher in the butanol (83.7%) and aqueous (80.4%) than in the ethanol extract (61.5%). And also, K562 showed similar tendency with KATOIII. The effects of Cordyceps militaris extracts on the frequencies of micronucleated polychromatic erythrocytes (MNPCEs) induced by N-methyl-N'-nitro-N-nitrosoguanidine(MNNG) were investigated in the bone-marrow cells of ICR male mice. The amount of 10, 20, 40 and 80 mg/kg of each extract were administered to animals immediately after injection of MNNG, and the exposure time was 36 hours. Significant reductions (p<0.05) with 39.7%, 52.7%, 71.4% and 83.9% were observed in the frequencies of MNPCE when 10, 20, 40 and 80 mg/kg of the hexane fraction of Cordyceps militaris extracts were given to the mice.
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함승시,김미남,정용진,이득식 동아시아식생활학회 2001 동아시아식생활학회지 Vol.11 No.1
This study was performed to examine the antimutagenic and cytotoxic effects of potato vinegar and commercial vinegars(cider, brown rice, persimmon vinegars) on Salmonella typhimurium TA98. TA100 and cancer cell lines using Ames test and cytotoxicity assay, respectively. In Ames test, all vinegars did not exhibit any mutagenicity, but showed substantial inhibitory effects against N-methyl-N-nitro-N-nitrosog-uanidine(MNNG), 4-nitroquinoline-1-oxide(4NQO), 3-amino-1.4-dimethyl-5H-pyrido[4,3-b] indol(Trp-P-1) and benzo(a)pyrene(B(a)P). The number of revertants per plate decreased significantly when these vinegars(80㎍/plate) were added to the assay system using TA100 strain. Especially, potato vinegar(80㎍/plate) showed high inhibition rate of 69.9% against mutagenicity of B(a)P on TA100 strain. In the cytotoxicity assay, these vinegars also showed prominent cytotoxic activity against human cancer cell lines. Potato vinegar(10㎍/showed the strongest cytotoxic effect against HT1080 (fibrosacoma cell) and K562 (myelogenous leukemia) at the same concentration when compared with other vinegars.
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