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온위탕(溫衛湯)이 ovalbumin에 의하여 유발된 allergic rat의 면역기능에 미치는 영향(影響)
한진근,김종한,박수연,최정화,Han, Jin-Kun,Kim, Jong-Han,Park, Su-Yeon,Choi, Jung-Hwa 대한한방안이비인후피부과학회 2005 한방안이비인후피부과학회지 Vol.18 No.1
Objective : This study was performed to investigate the effects of OWT on allergic bronchial asthma. Methods : The mice were divided into 4 groups induced the allergic bronchial asthma : Three groups(OWT-1, OWT-2, OWT-3) treated OWT and non-treated group(Control). Three oral administration of the herbal solution of OWT were carried out 1 lime a day for 2 weeks before antigen sensitization. 2 days later, the mice were sensitized with a subcutaneous injection of ovalbumin(OA) and then 13 days later they were provoked with OA aerosols. Then the cell numbers in bronchoalveolar lavage fluid(BALF), serum level of IgE, WBC, RBC and HCB were measured. Results : 1. On the neutrophil in BALF, OWT-1 group is significantly increased compared with the control group. 2. On the eosinophil in BALF, OWT-1 group tends to decrease compared with the control group but insignificant. 3. On the lymphocyte in BALF, OWT-2 group is significantly decreased compared with the control group. 4. On the macrophage in BALF, OWT-2 group is significantly increased compared with the control group. 5. On the serum IgE, groups OWT-1, OWT-2 and OWT-3 are significantly decreased compared with the control group. 6. On WBC in blood, OWT-1 group is significantly decreased compared with the control group. Conclusions : Based on the above results, it is assumed that the oral administration of OWT can help the treatment of allergic bronchial asthma.
유백피(楡白皮) 추출물이 인간의 피부 세포 재생 및 악성 흑색종 세포에 미치는 영향
한진근,박수연,김종한,최정화,Han, Jin-Geun,Park, Su-Yeon,Kim, Jong-Han,Choi, Jeong-Hwa 대한한방안이비인후피부과학회 2007 한방안이비인후피부과학회지 Vol.20 No.3
Objective : Cortex of Ulmi pumilae(CUP) has been used to treat several diseases including boil, swelling, and scabies etc. Recently, CUP was known to have wrinkle care and whitening actions. But, It's exact mechanisms are unclear. Methods : The present study was designed to investigate effects of CUP on Human HaCaT keratinocyte and malignant melanoma cells such as SK-MEL-2 and B16F10 in terms of cell viabilities, proliferations, DPPH free radical scavenging activities, oxygen free radical productions and inhibitory action on elastase activities. Results : CUP accelerated proliferation of HaCaT keratinocytes in the lower concentration. CUP also prevented cell death of HaCaT induced by Hydrogen peroxide, which products oxygen free radicals. On the contrary, CUP did not affect proliferations of SK-MEL-2 or B16F10. Futhermore, CUP showed inhibitory action against SK-MEL-2 proliferation at the concentration of $500{\mu}g/m{\ell}$ In addition, CUP was shown to have DPPH free radical scavenging activities and also have inhibitory effects on elastase activities too. On the fluorescent examinations, the present author knows that CUP elevated production levels of oxygen free radicals in malignant melanoma cell, SK-MEL-2. Conclusions : These results suggest that CUP has possibilities of usage for functional cosmetics which have wrinkle care and whitening activities and related mechanisms are involved in inhibition of elastase action and acceleration of oxidative stress in melanoma cell.
소아 성장에 미치는 한방요법의 임상적 효과 및 성장 진단척도로서의 골밀도 평가
김세진 ( Se Jin Kim ),고홍개 ( Min Kyu Chu ),최진봉 ( Jin Keun Han ),추민규 ( Hong Gae Ko ),한진근 ( Mi Suk Shin ),신미숙 ( Sun Jong Kim ),김선종 ( Jin Bong Choi ) 한방재활의학과학회 2007 한방재활의학과학회지 Vol.17 No.2
Objectives : The aim of the present study was to evaluate the effect of oriental medicine therapy on the growth of children, and to assess the relation of BMD(Bone Mineral Density) and the stature growth during the period of bone growth. Methods : We measured height and weight of the children, percentile of the growth by using the growth curve, BMD and bone age by using Osteoimages Plus. We treated the children with acupuncture, herbal acupuncture, herbal medication, physical therapy. Then we measured difference of height and percentile change of the children. Results and Conclusions : 1. According to sexual type, percentile change of the growth is higher in the female. 2. According to age group, percentile change of the growth is higher in period Ⅱ(Male;11-14, Female;10-12). 3. According to treatment group, percentile change of the growth is higher in acupuncture and herbal-acupuncture, herbal-medication treated group. 4. According as BMD increases, BMI(Body Mass Index) were ascending significantly.