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우리나라 가공식품(加工食品)중의 Clostridia perfrigens의 분포(分布)
한왕수,조양자,권종규,서인수,Han, Wang-Soo,Cho, Yang-Ja,Kwon, Chong-Kyu,Suh, Inn-Soo 대한미생물학회 1976 大韓微生物學會誌 Vol.11 No.1
A total of 100 swelled, springered or flippered canned meat and fish products were studied the degree of contamination with clostridias and serological relationships to Hobbs'13 "heat resistant" types, heat resistance of spores and susceptibility of Clostridium perfringens isolates to several antibiotics. Samples examined in this study were collected from Seoul area from June to October, 1975 and prepared in Korea. Clostridias were isolated from 46(46%) of these samples; 19 strains of Cl. perfringens, 9 strains of Cl. oedematiens A, B, 5 strains of Cl. sordelli, each 3 strains of Cl. chauvoei, Cl, oedematiens C.E, and Cl. difficile, 2 strains of Cl. sporogenes. The highest percentage of contamination by Cl. perfringens was found in beef products(26.5%), and the following(5.2%) in mackerel pike and none in baitop shell. whale, manna brand. and top shell. One of 19 isolates of Clostridium perfringens found in meat products was shown to produce heat resistant spores which resist $100^{\circ}C$ for 60 minutes and others were heat labile strains which is killed at $90^{\circ}C$ for 30 minutes. The distribution of Hobbs' serotype of 19 isolates were each 4 strains of type 6, 8, and 11, 1 strain of type 13 and others untypable. 19 Strains of Cl. perfringens were shown a marked susceptibility to cefamezin, lincomycin and minocin and relatively sensitive to vibraimycin, geopen, and chloramphenicol. A marked resistance to kanamycin, colimycin, and gentamycin were shown. Aerobic enteropathogens from samples were not recovered.
안병수,김경희,한왕수,서인수,Ahn, Byung-Soo,Kim, Kyung-Hee,Han, Wang-Soo,Suh, Inn-Soo 대한미생물학회 1987 大韓微生物學會誌 Vol.22 No.2
The incidnce of enterotoxigenic Esherichia coli(ETEC) was investigated in E. coli strains isolated from Korean infants less than two years old. Over a period of 12 months, ETEC strains have been isolated from 45(45.0%) of 100 children with acute diarrhea and from 9(20.5%) of 44 children without diarrhea. In the group with diarrhea, 41(41.0%) strains produced heat-stable toxin, 3(3.1%) produced heat-labile toxin, and 1(1.0%) produced both heat-stable and heat-labile toxins. In the control group, 7(15.9%) released heat-stable toxin, 2(4.5%) released heat-labile toxin and none released both. A statistical association of strains releasing heat-stable toxin was significant(P<0.025).
한왕수 한양대학교 의과대학 1983 한양의대 학술지 Vol.3 No.1
Seventy eight Shigella were isolated in Seoul area during a period from 1980 to 1981 and they were classified by means of biochemical and serological test. These strains originated from patients who had clinically been diagnosed as bacillary dysentery and other gastrointestinal complaints. Sensititivity test to 12 kinds of antimicrobial agents were carried out by means of Agar plate dilution technique in trypticase soy agar. Readings were made after 24 hours at 37℃ and determined by the criteria of complete inhibition of growth of test organism. A strain was regarded as resistant if it's growth was inhibited in ≥100mcg/ml of Tetracycline(Tc), Chloramphenicol(Cp), Streptomycin(Sm), Sulfanilamide(Sa), and Ampicillin(Ap), ≥25Mcg/ml of Kanamycin(Km), Cephaloridine(Cep), Furazolidone(Fz), Nitrofurantion(Nf), and Nalidixic acid(Na) and ≥6.25mcg/ml of Gentamicin(Gm), of total strains of 64 belonging to Shigella(Sh) flexneri and 14 to Sh. sonnei. The susceptibilities of organism to 12 drugs examined were found to be the highest to Km and Na, and lowest to Sa and Tc. In other hand, the susceptibilities to Tc, Cp, Sm, and Sa for Sh. flexneri strains were shown to be higher than that of Sh. sonnei strains. Shigella species showed a more significant resistance to Tc(84.6%), Cp(74.4%), Sm(80.8%), and Sa(94.7%), and showed a resistance to Nf(76.9%), Ap(62.8%), Cep(26.9%), Fm(23.1%), Gm(10.3%), Fz(10.3%), Na(6.4%), Km(0.0%) in order it was also found that there is marked difference between Sh. flexeri and Sh. sonnei to Sm, Sa, Ap and Tc, Cp pattern. The most predominant and remarkable differences were in thge frequency of these resistance among Sh. flxneri and Sh. sonnei strains. The multiple drug-resistant strains which were shown to be resistant to Tc, Cp, Sm, Ap or Cep were also resistant to Nf, Cep and Sa.
Campylobacter pylori 위장내 분리위치에 따른 병원성
한왕수,한상진,김정목,조양자 대한감염학회 1989 감염 Vol.21 No.1
Compylobacter pylori may cause gastritis and has been proposed as an etiologic factor in the development of peptic ulcer. However, it is unknown where is primary colonization sites of C. pylori. Isolation sites of C. pylori in stomach and duodenum and pathology in antrum were evaluated in 90 subjects endoscoped at Hanyang University Hospital in Seoul, Korea. C. pylori detected in biopsied specimens of 40 (74%) of 54 C. pylori-positive patients at antrum only, and 13 (24%) of the same patients at antrum and body, but did not detected in duodenal biopsied specimens. Antral pathology results were chiefly found in active chronic gastritis. These findings strongly suggest that antrum is a primary colonization sites of C. pylori and the agent may be spread within stomach from the antrum.
정용훈,조양자,박장환,한왕수,김광남 한양대학교 의과대학 1991 한양의대 학술지 Vol.11 No.2
Exotoxin A from Pseudomonas aeruginosa is known to be the most lethal and the most extensively studied product among its virulence factors. Hybridomas secreting monoclonal antibodies against exotoxin A from Pseudomonas aeruginosa PA 103 were derived from the fusion of myeloma cell with spleen cells from mice immunized with purified exotoxin A. Total of 582 hybridomas were screened and selected by using an enzyme-linked immunosorbent assay. Among these 39 clones positive for binding to exotoxin A were obtained and fusion-specific efficiency was turned out to be 6.7%. All antibody isotypes were represented (immunoglobulins G, and M) as determined by sandwich enzyme-linked immunosorbent assay. Hybridomas were selected and cloned by limiting dilution and agarose methods, obtaining 1 IgG? and 11IgG? clones. The specificity of the monoclonal andtidodies for exotoxin A was demonstrated by the immunoblotting technique. They bound 66kilodalton (KDa) exotoxin band and 45KDa fragment B portion. The protective ability of anti-exotoxin A monoclonal antibody was demonstrated in vitro by toxin neutralization. Ascitic fluid of monoclonal antibody producing hybridoma was able to neutralize at maximum 100 dilution to CHO cell cytotoxicity by 2ng of Pseudomonas ceruginosa exotoxin A and 60% increase in survival rate has been obtained through passive immunization by using anti-exotoxin A monoclonal antibody (IgG?) in mice injected 300ng of exotoxin A. And its in vivo and in vitro protective activities were appeared to reside in tis selective affinity and specificity to fragment B of Pseudomonas aeruginosa exotoxin A.