Perchloric acid 가용성 Mycobacterium tuberculosis 항원이 말초혈액 림프구 증식에 미치는 영향

한부현(Boo Hyun Han),김동수(Dong Soo Kim),이기영(Ki Young Lee) 대한소아알레르기호흡기학회(구 대한소아알레르기 및 호흡기학회) 1994 소아알레르기 및 호흡기학회지 Vol.4 No.2

N/A Mycobacterium tuberculosis has very complex antigenic structures and large number of antigenic determinants. Due to recent development of immunology, the study on the role of each antigen and its mutual response in human immune system by extracting antigens from the acid-fast bacillus have undergone rapid development. Also, certain types of antigen have been studied on how they are related to antibody and cell mediated immune response by revealing molecular structures. In that regard, the authors investigated its antigenicity from such antigen by extract antigen(TB-PBE) using perchloric acid, ethanol, and boiling out of M. tuberculosis. TB-PBE was composed of only one antigenic band, molecular weight of 58kDa. A 58kDa M. tuberculosis antigen(TB-PBE) stimulated peripheral blood lymphocytes of normal adult for a period from the 1st to the 14th at various times(lst, 2nd, 3rd, 4th, 7th, 9th, 10th, 14th day) in various concentrations (10, 1, 0.1, 0.01pg/ml) and after the stimulation, mitogenesis was measured by H-thymidine and measured B cell, T cell, CD4, CD8, DR, y T cell. ELISA was used to measure IL-2 and IL-4. TB-FBE showed the strongest stimulus effect 7 days after the stimulation. After the stimulation, no change in B and T cells was observed but the number of CD3+ and DR+ cells increased and observed more activities of CD4 T cells due to increase in the ratio of CD4/CD8, Only IL-2 could be measured in cultured supernatant and IL-4 was not observed. TB-PBE antigen showed stimulatory effect on CD4 T cells in peripheral lymphocytes and caused more activation of Th1 than Th2. TB-PBE antigen exists on the surface of M, tuberculosis and induces only cellular immunity.

위장관 ( 胃腸管 ) : 소화기계의 선천성 기형에 대한 임상통계적 관찰 - 최근의 변화 양상 -

정기섭(Ki Sup Chung),한부현(Boo Hyun Han),김문규(Moon Kyu Kim) 대한소화기학회 1990 대한소화기학회지 Vol.22 No.3

N/A A clinicostatistical review was done on 505 infants and children diagnosed and treated at the Department of Pediatrics and Surgery in Severance Hospital for congenital digestive tract anomalies from January, 1979 to December, 1988. The results are summarized as follows; 1) There was 505 cases admitted with congenital digestive tract anomalies occupying 1.61% of all pediatric patients admitted during the period from 1979 to 1988. This was a double-fold increaae in the number of patients as compared to the previous 250 cases. The male to female ratio was 1.8:1 which was similar to the ratio of 2.0:1 between 1966 and 1975. 2) The most common congenital anomaly was congenital hypertrophic pyloric stenosis with 122 cases (24.2%), followed by imperforate anus with 106 cases (21.0%), congenital megacolon with 104 cases (20.6%) and congenital hepatobiliary anomalies with 51 cases (10.1%). In the report of 1966~75, congenital megacolon was the most frequent anomaly followed by imperforate anus and congenital hypertrophic pyloric stenosis. There were also 14 cases of midgut malrotation and 5 cases of gastroschisis which were not present in the previous report. On the otber hand, the number of cases with Meckel's diverticulum had decreased (8 cases) since the previous report (18 cases). 3) Among 37 cases of congenital anomalies of small and large intestine, 19 cases (51.4%) were ileal atresia followed by 7 cases (18.9%) of duodenal atresia. 4) Associated anomalies were observed in 66.0% to 85.7% of cases with situs inversus, imperforate anus and gastroschisia However, the overall incidence of congenital malformations was 25.0% which was not significantly different from that of the previous study (22.8%). When the incidence of associated anomalies were analyzed with the previous report, it was noted that there was a rise in the incidences of associated anomalies in imperforate anus from 17.8% to 66.0% and in congenital hepatobiliary anomalies from 12.0% to 21.6% while a fall in incidences was noted in congenital megacolon from 12.0% to 4.8%, in congenital hypertrophic pyloric stenosis from 9.7% to 4.1%, in congenital intestinal anomalies from 41.4% to 5.9% and in situs inversus from 100% to 85.% 5) Surgery was done in 419 cases. Among them 26 cases (6.2%) expired which was a significant decrease in mortality rate as compared to previous report (19.3%). When the mortality rate of each disease was compared to that of the previous report, the mortality rate of imperforate anus decreased from 14.3% to 1.0%, that of congenital megacolon from 15.6% to 2.5%, congenital hepatobiliary anomalies from 47.6% to 5.3% and congenital intestinal anomalies from 40.7% to 18.8%. 6) The most common contributing factor to death was respiratory failure (73.0%) as in the previous study. Sepsis was also a common contributing factor to death.

Interleukin - 6 가 배양된 정맥내피세포에서 E - selectin 의 표현에 미치는 영향

김동수(Dong Soo Kim),한부현(Boo Hyun Han),이기영(Ki Young Lee) 대한소아알레르기호흡기학회(구 대한소아알레르기 및 호흡기학회) 1995 소아알레르기 및 호흡기학회지 Vol.5 No.1

N/A Interleukin-6(IL-6) is a pleomorphic cytokine , which has a variety of functions including stimulatory effect of hepatocytes to produce acute phase reactants . With interleukin - l(IL - 1) and tumor necrosis factor - a( TNF ? A ) , IL-6 has a function in acute inflammation. We conducted this study to clarified that IL-6 also has a function to stimulate the expression of E-selectin on cultured HUVEC like IL-1 and TNF-a. The results were as follows ; l. IL-6 had a stimulatory effect to express E - selectin on HUVEC with dose-dependent pattern. 2. A peak stimulatory effect of IL - 6 on expression of E - selectin was 24 hours after incubation and then decreased gradually. 3. This stimulatory effect of IL - 6 on expression of E-selectin was blocked by mouse anti-IL-6 monoclonal antibody. 4. A stimulatory effect of IL-6 on E-selectin expression could be also observed on FACS analysis. 5. When incubated with IL - 6 for 16 hours , the morphology of HUVEC showed shrunken cells, vacuoles in cytoplasms , and some necrosis which looked like a inflammatory response .

운동유발성 천식에 대한 흡입용 BETA2 - AGONIST , 경구용 BETA2 - AGONIST , CROMOLYN SODIUM 및 준비운동 ( WARM - UP ) 의 예방 효과에 관한 교차 비교 연구

김규언(Kyu Earn Kim),강혜영(Hye Young Kang),한부현(Boo Hyun Han),이수영(Soo Young Lee),정병주(Byung Ju Jeoung),이기영(Ki Young Lee) 대한소아알레르기호흡기학회(구 대한소아알레르기 및 호흡기학회) 1994 소아알레르기 및 호흡기학회지 Vol.4 No.2

N/A EIA represents a transient airflow obstruction following strenuous exercise and can cause physical and psychological problems in children. It is very important to prevent EIA with pharmacologic or non-pharmachologic methods. To compare the preventive effects of such methods, it is essential that these methods be tried for same individual. We compared the preventive effects of inhaled beta2-agonist, oral beta2-agonist, cromolyn sodium and warm-up by cross-over study on EIA in 45 children (27 boys and 18 girls aged 6 to 15 years). They were diagnosed as EIA by exercise loading test (up and down stairs method for 6 minutes). The changes in pulmonary function were measured by peak expiratory flow rate (FEFR). The results are as follows: 1. The preventive effects were 93.3% in inhaled beta2-agonist, 66.7% in warm-up, 62.2% in cromolyn sodium, and 33.3% in oral beta2-agonist. 2. When we analyzed the preventive effects according to the severity of EIA, we had the following results: warm-up, cromolyn sodium, and oral beta2-agonist were only effective in mild EIA, while inhaled beta2-agonist was effective in all cases of EIA. In conclusions, regardless of severity of EIA inhaled beta2-agonist was the most effective in the prevention of EIA. And warm-up and cromolyn sodium were followed. Oral beta2-agonist was effective only in mild cases of EIA.

Seckel증후군(Bird-headed dwarfism)

한부현,고창준 대한의학협회 1990 대한의학협회지 Vol.33 No.11