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TNF-α로 유도된 혈관내피세포의 혈관염증에 미치는 오적산(五積散)의 억제 효과
한병혁 ( Byung Hyuk Han ),윤정주 ( Jung Joo Yoon ),김혜윰 ( Hye Yoom Kim ),안유미 ( You Mee Ahn ),홍미현 ( Mi Hyeon Hong ),손찬옥 ( Chan Ok Son ),나세원 ( Se Won Na ),이윤정 ( Yun Jung Lee ),강대길 ( Ho Sub Lee ),이호섭 대한본초학회 2018 大韓本草學會誌 Vol.33 No.4
Objectives : Ojeoksan, originally recorded in an ancient Korean medicinal book named “Donguibogam” and has been used for the treatment of circulation disorder of blood which was called blood accumulation (血積) in Korean medicine. Therefore, this study was carried out to investigate the beneficial effect of OJS on vascular inflammation in HUVECs. Methods : We evaluated the effect of OJS on the expression of cell adhesion molecules and protective role in HUVEC stimulated by TNF-α by using Western blot. Results : Pretreatment with OJS decreased the adhesion of HL-60 cells to TNF-α-induced HUVEC. OJS suppressed TNF-α-induced expression level of cell adhesion molecules such as intracellular adhesion molecule-1 (ICAM-1), vascular cell adhesion molecule-1(VCAM-1), and endothelial cell selectin (E-selectin). Moreover, OJS significantly decreased TNF-α-induced production of intracellular reactive oxygen species (ROS); and inhibited the phosphorylation of IκB-α in the cytoplasm compared to the experimental group. Pretreatment with OJS inhibited the trans-location of NF-κB p65 to the nucleus. OJS also inhibited phosphorylation of MAPKs compared to the experimental group. OJS significantly increased the protein expression of Nrf2 and HO-1. Conclusions : Ojeoksan has a protective effect on vascular inflammation, and might be a potential therapeutic agent for early atherosclerosis.
위령탕(胃?湯) 추출물의 사람 유래 신장 메산지움 세포에서의 당뇨병성 신장 손상 개선 효과
윤정주 ( Jung Joo Yoon ),한병혁 ( Byung Hyuk Han ),최은식 ( Eun Sik Choi ),남궁승,( Seung Namgung ),정다혜 ( Da Hye Jeong ),김혜윰 ( Hye Yoom Kim ),안유미 ( You Mee Ahn ),이윤정 ( Yun Jung Lee ),강대길 ( Dae Gill Kang ),이호섭 ( H 대한본초학회 2016 大韓本草學會誌 Vol.31 No.5
Objectives : Diabetic nephropathy is one of the most common chronic complications of diabetes and a leading cause of end-stage renal failure in the world. Mesangial cell proliferation is known as the major pathologic features such as glomerulosclerosis and renal fibrosis. Wiryeongtang (WRT) is a well-known traditional herbal formula as therapeutic agents for chronic edema and dysuresia of renal homeostasis. In the present study, we investigated whether WRT inhibits high glucose (HG)-induced renal dysfunction by TGF-β/Smads signal regulation in cultured mesangial cells. Methods : Inhibitory effect of WRT (10-50 ㎍/ml) on HG-stimulated mesangial cells proliferation and dysfunction were evaluated by [3H]-thymidine incorporation, Western blot, and RT-qPCR. Results : WRT significantly decreased HG-accelerated thymidine incorporation in human renal mesangial cell in a dose-dependent levels. WRT induced down-regulation of cyclins/CDKs and up-regulation of CDK inhibitor, p21waf1/cip1 and p27kip1 expression. In addition, HG enhanced expression of dysfunction biomarker such as collagen IV and CTGF, which was markedly attenuated by WRT. WRT decreased TGF-β1 and Smad-2/Smad-4 expression, whereas increased Smad-7 expression under HG. Furthermore, WRT inhibited HG-induced inflammatory factors level such as ICAM-1 and MCP-1 as well as NF-κB p65 nuclear translocation and intracellular ROS production. Conclusions : These results suggested that WRT may alleviate mesangial proliferation and inflammation possibly involved in renal fibrotic process, further diabetic nephropathy through disturbing TGF-β1/Smad signaling and NF-κB/ROS pathway. Thus, WRT might prove to be effective in the treatment of renal dysfunction leading to diabetic nephropathy.
Puromycin Aminonucleoside에 의해 유도된 신증후군에 대한 옥미수(玉米鬚)의 보호효과
윤정주 ( Jung-joo Yoon ),고민철 ( Min-chol Kho ),한병혁 ( Byung-hyuk Han ),김혜윰 ( Hye-yoom Kim ),안유미 ( You-mee Ahn ),이윤정 ( Yun-jung Lee ),이호섭 ( Ho-sub Lee ),강대길 ( Dae-gill Kang ) 대한한방부인과학회 2021 大韓韓方婦人科學會誌 Vol.34 No.4
Objectives: Nephrotic syndrome is a kidney disorder, which is characterized by proteinuria, edema (swelling), and hyperlipidemia. Maydis Stigma (Corn silk) has been widely used in Asia as a traditional medicine and is known to have a diuretic effect and is used for the treatment of edema and indigestion. Methods: The aim of this study is to investigate the improvement effect of Maydis Stigma in treating nephrotic syndrome induced by puromycin aminonucleoside. Sprague-Dawley rats were intravenously injected with 75 mg/kg/day puromycin aminonucleoside, then treated with either Losartan or 200 mg/kg/day Maydis Stigma for seven days. Results: Maydis Stigma significantly decreased ascites and proteinuria level. Plasma levels of blood urea nitrogen (BUN) and plasma creatinine reduced significantly by Maydis Stigma. In addition, treatment with Maydis Stigma attenuated histological damage. Treatment with Maydis Stigma also restored podocin expression and reduced inflammation markers such as intracellular adhesion molecules (ICAM-1), monocyte chemotactic protein-1 (MCP-1), tumor necrosis factor alpha (TNF-α) and high-mobility group box-1 (HMGB1). Conclusions: Maydis Stigma ameliorates kidney injury in nephrotic syndrome rat models. Maydis Stigma exerts a renoprotective effect owing to its anti-inflammatory effects and reductions of ascites and proteinuria. Thus, these results indicate that Maydis Stigma is likely to be a promising agent in the treatment of nephrotic syndrome.
오령산이 허혈-재관류로 유발된 급성 신부전 백서에 미치는 효과
박지훈(Ji Hun Park),윤정주(Jung Joo Yoon),한병혁(Byung Hyuk Han):김혜윰(Hye Yoom Kim),황진석(Jin Seok Hwang),이윤정(Yun Jung Lee),이호섭(Ho Sub Lee),강대길(Dae Gill Kang) 대한약학회 2018 약학회지 Vol.62 No.1
Renal ischemia-reperfusion injury(IRI), an important cause of acute renal failure(ARF), increases renal tubular injury. Oryeongsan(OS), the oriental chinese herbal formula, widely used for the treatment of nephrosis, dropsy and uremia. This study investigated the effect of OS on rat model of ischemic ARF. Ischemia was induced by clamping the two renal arteries for 45 min followed by reperfusion. Peritoneal injection of 10 and 50 mg/kg/day OS for ischemia-reperfusion injury rat and IRI injury was measured 4 days after OS treatment respectively. Treatment with OS significantly ameliorates urine volume, urine osmolality, creatinine clearance(Ccr) and blood urea nitrogen(BUN) of kidney index. In addition, OS increases the expression aquaporine 2(AQP 2), Na + -K + ATPase and urea transporter(UT-B). Histopathologic studies have shown that OS improves glomerular expansion and tubular damage in the kidney cortex and medulla. These findings suggest that OS ameliorates tubular injury including tubular dysfunction in IRI induced ARF rats.