http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
황재규 ( Jae Gyu Hwang ),윤종국 ( Jong Kuk Yun ),한길환 ( Kil Hwan Han ),도은주 ( Eun Ju Do ),이진상 ( Jin Sang Lee ),이은주 ( Eun Ju Lee ),김종부 ( Jong Boo Kim ),김미려 ( Mi Ryeo Kim ) 대한본초학회 2011 大韓本草學會誌 Vol.26 No.1
Objectives: The present study was designed to investigate effects of mixed extract from korean medicinal herbs (MIX) on oxidation/reduction reaction-related and aging-related enzyme in vitro. Methods: We performed MTT assay, collagenase inhibition assay, elastase inhibition assay, tyrosinase inhibition assay, DPPH free radical scavenging assay, SOD-like activity and xanthine oxidase inhibition assay. Results: Recently, many studies have reported that elastin is also involved in inhibiting or repairing wrinkle formation, although collagen is a major factor in the skin wrinkle formation. The MIX showed 97% inhibition of collagenase activity, and 64% inhibition of elastase activity at 1 mg/ml concentration of MIX, next only to positive control, which indicate good efficacy for anti-wrinkle ingredient. Also it`s treatment showed 34% inhibition of tyrosinase activity, to relate whitening effect, at the same dose of MIX. Antioxidant activities were determined by DPPH radical scavenging, xanthine oxidase (XO) inhibiting activity and SOD-like activity. Also these scavenging, XO-inhibiting and SOD-like activities were measured in 91%, 80%, and 63% inhibition, respectively, at a treated dose of 1 mg/ml, compare to control. Conclusions: These results suggest that possibility of mixed korean medicinal herbs as a functional ingredient for anti-wrinkle and whitening, anti-oxidation and anti-aging cosmetic formula.
쌍화탕 추출물이 항산화효소 및 항노화관련 효소 활성에 미치는 영향
박지영 ( Ji Young Park ),황재규 ( Jae Gyu Hwang ),윤종국 ( Jong Kuk Yun ),한길환 ( Kil Hwan Han ),도은주 ( Eun Ju Do ),김성옥 ( Sung Ok Kim ),김미려 ( Mi Ryeo Kim ) 대한본초학회 2012 大韓本草學會誌 Vol.27 No.3
Objectives: The present study was designed to investigate effects of Ssanghwa-tang (Shuanghua-geng) on oxidation/reduction reaction-related and aging-related enzymes in vitro. Methods: We performed MTT assay, collagenase inhibition assay, elastase inhibition assay, tyrosinase inhibition assay, DPPH free radical scavenging assay, SOD-like activity and xanthine oxidase (XO) inhibition assay, Results: The 50% ethanol (EtOH) extract of Ssanghwa-tang (SHT) showed 55% inhibition of collagenase activity, and 42% inhibition of elastase activity at 1 mg/ml concentration, Also it`s treatment showed 18% inhibition of tyrosinase activity, to relate whitening effect, at the same dose of 50% ethanol extract of SHT. Antioxidant activities were determined by DPPH radical scavenging, XO inhibiting activity and SOD-like activity. These scavenging, XO-inhibiting and SOD-like activities were measured in 80%, 75%, and 28% inhibitions, respectively, at a 1 mg/ml treated dose, compared to those of control. The inhibitory effects of 50% EtOH extract on aging and oxidation-related enzyme activities were higher than those of water extract and 95% EtOH extract. Conclusions: Taken together, our findings suggest that the SHT has potential and applicable benefits for development of cosmetics to have anti-aging (anti-wrinkle and whitening) and anti-oxidation functions.
황재규 ( Jae Gyu Hwang ),윤종국 ( Jong Kuk Yun ),김숙경 ( Suk Kyung Kim ),이상한 ( Sang Han Lee ),한길환 ( Kil Hwan Han ) 한국미생물생명공학회(구 한국산업미생물학회) 2012 한국미생물·생명공학회지 Vol.40 No.3
Chaff-vinegar is known for having a variety of useful purposes in the fields of health and lifestyles. In a previous study we isolated and identified the active fractions of the polyphenol com-pound 7 species as a potential biomaterial for cosmeceuticals. To further test for its potential use as a functional material, we carried out an MTT assay, collagenase inhibition assay, elastase inhibition assay, tyrosinase inhibition assay, DPPH free radical scavenging assay, SOD-like activity assay and a xanthine oxidase inhibition assay. Chaff-vinegar exhibited potent collagenase and elastase inhibitory activities in a concentration dependent manner, indicating that the agent has the potential to alleviate the skin wrinkling process. Chaff-vinegar also showed80% tyrosinase inhibition at a concentration of 100μL/mL. DPPH radical scavenging, xanthine oxidase inhibition, and SOD-like activity results for each activity were 80%, 80%, and 100%, respectively. Taken together, the present study suggests that chaff-vinegar is a good candidate for use as an anti-wrinkling and/or whitening agent.
Streptomyces Iuteogriseus KT-10이 생산하는 Cathepsin B 저해물질의 분리 및 특성
한길환,김상달 한국산업미생물학회 2001 한국미생물·생명공학회지 Vol.29 No.2
포유동물조직의 세포내에 존재하는 lysosomal cysteine계 cathepsin B 효소는 암 발병과 전이, 류머티스 관절염, 염증 및 노인성치매 등의 여러 질병에 관여하고 있다. 이 cathepsin B를 저해하는 저해물질이 Streptomyces luteogriseus KT-10에 의해 생산되어 분리되었다. S. luteogriseus KT-10이 생산하는 cathepsin B 저해물질은 열에 안정하며 산, 알칼리에도 안정한 물질로 구성되어 있다. Cathepsin B 저해물질은 DEAE-Sephadex A-25, Sephadex G-15, silica gel, Sephadex LH-20의 column chromatography 과정을 거친 후 분취용 HPLC를 이용 분취한 후 백색분말의 cathepsin B 저해물질을 정제하였다. 이 정제한 저해물질은 UV 상의 전 범위에서 특이한 검출부위를 확인 할 수 없었으며, H_2O, methanol, ethanol, n-butanol에는 녹지만 비극성인 chloroform, n-hexane, benzene 등에는 불용성이었다. 또한 ninhydrine, H_2SO_4, iodine에 양성 반응이지만 Ehrlich's, Pauly, Sakaguchi, phthalic acid, DNS, aniline 등의 단백 반응과 당 반응에는 음성 반응으로 나타났다. IR 기기에서는 OH 기와 CH 기의 peak를 확인하였으며 ^1H NMR 기기로 OH peak와 NH peak 또한 확인 할 수 있었다. ^13C NMR spectrum은 4개의 탄소 peak를 가진 물질로 확인된 분자량이 207 dalton인 저해물질이다. 원소 분석기를 이용한 저해 물질 분석 결과 분자식이 C_4H_11O_4N_6로 나타났다. 이 cathepsin B 저해물질의 저해양식은 competitive 저해로 작용함을 확인 할 수 있었다. The cathepsin B inhibitor produced by Streptomyces luteogriseus KT-10 was very stable in heat, acidic and alkaline conditions. The cathepsin B inhibitor was isolated from the extracted fraction of culture broth with butanol, methanol and choloroform subsequently, the inhibitor was purified with following several column chromnatography such as DEAE-Sephadex A-25, Sephadex G-15, silica gel 60, Sephadex LH-20, and preparative HPLC. The cathepsin B inhibitor showed positively to detective reaction of ninhydrine, 5% H_2SO_4, iodine, but negatively to the reaction of Ehrlich's reagent, DNS, aniline. The molecular formular of cathepsin B inhibitor was elucidated by IR, ^1H and ^13C-NMR, FAB mass and elemental analyzer. Consequently, it was identified as C_4H_11O_4N_6. The cathepsin B inhibitor had the mode of competitive inhibition with the reaction of cathepsin B.
Cathepsin B 저해물질을 생산하는 Streptomyces misakiensis의 동정 및 저해물질의 분리
한길환,김상달 한국산업미생물학회 2001 한국미생물·생명공학회지 Vol.29 No.1
세포내의 lysosomal cysteine계 단백질분해 효소 cathepsin B는 기저막을 분해하여 암전이 및 염증반응의 원인이 되고 면역작용에 관계하여 류머티즈 관절염 등의 질병과 노인성 치매 등의 발병에 관여하고 있는 단백질 분해효소이다. 본 연구는 토양으로부터 cathepsin B에 저해력을 가진 균주들을 선발하여 저해력이 가장 강한 한 균주를 선발하여 동정을 시도하였으며 그 결과 Streptomyces misakiensis로 동정하였다. S. misakiensis의 배양상 특성을 조사한 결과 28℃에서 4일 배양했을 경우 균주의 저해물질 생산성이 가장 좋았으며 균체의 생육은 5일 배양했을 경우 가장 좋았다. 또한 균체의 배양시 배지내의 pH의 변화는 약산성으로 계속 pH가 감소하다가 6일 후 pH가 증가하였다. 저해물질의 정제과정은 n-butanol, methanol, chloroform의 추출과정과 Sephadex G-15, silica gel chromatography, Sephadex LH-20의 chromatography를 수행하여 최종적으로 preparative ODS-80TM column HPLC로써 정제를 하였다. Trypsin, papain, cathepsin B에 대해 저해력을 확인 할 수 있었으며 저해물질은 분자량이 138 정도의 매우 작은 물질로 이루어져 있음을 확인하였다. A strain of Actinomycetes producing cathepsin B inhibitor was isolated from soil and identified as Streptomyces misakiensis. The product of S. misakiensis inhibited effectively cathepsin B proteinases as well as trypsin and papain. The cathepsin B inhibitor were largely produced with incubation for 4 days. The S. misakiensis was the most growth with incubation for 5 days. The cathepsin B inhibitor was isolated from the extraction of both with butanol, ethanol and chloroform, and following several column chromatography such as sephadex G-15, silica gel 60 and sephadex LH-20 chromatography. The moleculer weight of purified inhibitor was 138 dalton.
세균성 Urease Gene에 의한 모기유충 방제균 Bacillus sphaericus 1593의 형질전환
한길환,김상달 한국산업미생물학회 1995 한국미생물·생명공학회지 Vol.23 No.4
위생곤충이나 농업해충의 방제목적으로 남용되는 화학살충제의 피해를 줄이기 위해 Bacillus sphaericus를 이용하는 생물학적 해충방제법의 연구가 진행되고 있다. 모기유충방제균인 B. sphaericus 1593의 살충단백질은 alkali 조건하에서 활성도가 증가된다. Urea의 효소적분해로 ammonia의 생산을 증강하여 독소단백질 주위의 pH를 알칼리화 할려는 시도를 하였으며, 이를 위해 세균성 urease의 생산유전자를 B. sphaericus 1593에 도입시키고자 하였다. Urease 유전자는 세균 중 urease 생산력이 가장 강한 Bacillus pasteurii의 urease 유전자를 cloning 한 재조합 plasmid pGU66을 사용하였다. B. sphaericus 1593 내에 있는 cryptic plasmid를 먼저 제거하고 원형질체 형질전환 방법으로 recombinant palsmid pGU66을 도입함으로서 80% 이상의 urease 활성 증가를 이룰 수 있었으며, 도입된 urease가 transformant 내에서 매우 안정하게 발현됨을 확인하였고, 아울러 urease 유전자로 형질전환된 B. sphaericus 1593(pGU66) 내에서도 모기유충 살충단백질이 변함없이 생산됨을 전자 현미경으로 확인할 수 있었다. Bacillus sphaericus 1593 is a larvicidal toxin-producing mosquitocidal bacterium. The toxin contains a parasporal crystalline inclusion which is composed of a protein that is activated under alkaline condition. To enhance alkaline environment around toxin protein, cryptic plasmid cured, B. sphaericus 1593 was transformed by the Bacillus pasteurii urease gene which generate ammonia from urea. Transformant produced urease at about 80% more than wild type strain. B. sphaericus 1593, and the urease gene was stably maintained. It also produced crystalline toxin protein at the same level as the wild type strain B. sphaericus 1593.