야생담배에서의 동질 머리핀 RNA의 일시 발현에 의한 오이모자이크바이러스 감염 저해

한경식 ( Kyoung Sik Han ),최승국 ( Seung Kook Choi ) 한국국제농업개발학회 2014 韓國國際農業開發學會誌 Vol.26 No.2

Cucumber mosaic virus (CMV), genus Cucumovirus, family Bromoviridae causes damage in many economically important horticultural and ornamental crops. Sequence alignments showed several conserved sequences sites in 3′-noncoding region (NCR) of CMV genomic RNAs in subgroup IA strains characterized so far. Based on this observation, we generated a hpRNA construct (pIRCMVNCR) harboring an inverted repeat containing a 162 bp cDNA fragment homologous to 3` NCR portion of CMV RNA3 to investigate the silencing potential for its ability to interfere with a rapidly replicating CMV. Agrobacterium-mediated transient expression of the pIR-CMVNCR had a detrimental effect on CMV infection, showing no distinct symptoms in non-inoculated leaves of the agroinfiltrated N. benthamiana plants. CMV genomic RNAs including subgenomic RNA4 were not detected by RT-PCR analysis from tissues of both the inoculated leaves and upper leaves of the agroinfiltrated plants. Accumulation of virus-derived small interfering RNAs was detected in the inoculated leaf tissues of N. benthamiana plants elicited by transient expression of pIR-CMVNCR indicating RNA silencing is responsible for the resistance to CMV.

노니의 기능 및 역할에 관한 연구

황효정,신경옥,한경식,Hwang, Hyo-Jeong,Shin, Kyung-Ok,Han, Kyoung-Sik 한국식품영양학회 2019 韓國食品營養學會誌 Vol.32 No.4

This study was carried out to investigate the various roles and effects of Morinda citrifolia L.(noni) on the human body and to utilize the findings obtained for future disease related research on food and nutrition. Morinda citrifolia L. has been used in folk medicine by Polynesians for over 2,000 years and is reported to have a broad range of therapeutic effects. Noni is an alkaloid system such as proxeronine, scopoletin, octanoic acid, terpenoids, alkaloids, anthraquinones, nordamnacanthal, morindone, rubiadin, b-sitosterol, flavone glycosides, linoleic acid, acubin, L-asperuloside, caproic acid, caprylic acid, ursolic acid, rutin. Noni's side effects have been reported to affect anti-inflammatory, analgesics, LDL antioxidant, anti-tumor, antifungal, antibiotic, antiviral, antiparasitic and immunosuppressive effects. In particular, noni's efficacy is considered to be important for the prevention of diseases by inhibiting active oxygen, which is a direct cause of oxidative stress, through various metabolites through 'xeronine system'. Noni's functions and effects that have been examined in this study include anti-inflammation, pain relief, antioxidant, anticancer, hepatocyte protection among others. Therefore, noni's extracts are considered highly useful for diverse scientific and nutritional health functional foods.

산양유 단백질 분해물/키토올리고당 나노 전달체 제조 및 물리화학적 특성연구

하호경,김진욱,한경식,윤숭섭,이미령,이원재,Ha, Ho-Kyung,Kim, Jin Wook,Han, Kyoung-Sik,Yun, Sung Seob,Lee, Mee-Ryung,Lee, Won-Jae 한국유가공학회 2017 한국유가공기술과학회지 Vol.35 No.3

본 연구에서는 산양유 단백질 분해물과 키토올리고당을 이용하여 약 138~225 nm 크기를 지니는 구형의 나노 전달체를 성공적으로 제조하였다. 제조된 나노 전달체는 소수성 건강기능성 물질인 DHA와 레스베라트롤을 각각 ~22 mg/100 mL와 ~4.5 mg/100 mL 씩 포집가능하며, 제조 공정요인(예, TPP 농도, 산양유 단백질 분해물 농도, 키토올리고당 농도) 조절을 통해 입자 크기, 표면전하등과 같은 나노 전달체의 물리화학적 특성과 소수성 건강기능성 물질의 포집 효율을 조절할 수 있음을 알 수 있었다. 또한 산양유 단백질 분해물/키토올리고당 나노 전달체는 동결건조를 통해 분말화가 가능하기 때문에 식품 적용성이 뛰어나다. 결론적으로 본 연구에서 food-grade 물질인 산양유 단백질 분해물과 키토올리고당을 이용하여 제조한 나노 전달체는 저지방 및 무지방 유식품에 적용 가능한 무지방 기반(non-fat based)의 소수성 건강기능성 물질 전달체로 이용될 수 있을 것으로 기대된다. The aims of this study were to manufacture a hydrolyzed goat milk protein (HGMP)/chitosan ologisaccharide (CSO) nano-delivery system (NDS) and to investigate the effects of production variables, such as sodium tripolyphosphate (TPP), HGMP, and CSO concentration levels, on the formation and physicochemical properties of the NDS. An HGMP/CSO NDS was produced using the ionic gelation method at pH 5.5. Transmission electron microscopy and a particle size analyzer were used to determine the morphological and physicochemical properties of NDSs, respectively. The size of the HGMP/CSO NDS decreased from 225 to 138 nm as HGMP and CSO concentration levels decreased. The NDS had a positive surface charge, with a zeta-potential value of +23 mV. The encapsulation efficiency (EE) of docosahexaenoic acid was enhanced as the HGMP concentration level increased. Additionally, increasing the concentration level of CSO resulted in an increase in the EE of resveratrol. The HGMP/CSO NDS exhibited good physical stability during freeze-drying. Thus, our findings showed that the HGMP/CSO NDS was successfully manufactured and that HGMP and CSO concentration levels were key factors affecting the physicochemical properties of the NDS.

혼합농축콩단백을 첨가한 대체 소시지의 이화학적 특성에 관한 연구

차서희,신경옥,한경식,Cha, Seo-Hui,Shin, Kyung-Ok,Han, Kyoung-Sik 한국식품과학회 2020 한국식품과학회지 Vol.52 No.6

본 연구는 우리나라에서 재배되는 대두, 녹두, 적두 및 완두 4종 콩 원물을 한 번에 추출하여 만든 혼합농축콩단백을 이용하여 대체 소시지를 개발하여 물리화학적 특성을 닭 소시지와 비교하였다. 경제성과 영양학적 특성을 고려하여 4종 콩 원물의 최적 혼합 비율을 설정하여 혼합농축콩단백을 추출하였을 때 혼합 농축단백 100 g을 기준으로 한국인 성인 남성 기준의 필수 아미노산 하루 평균 필요량이 충족되었다. 혼합농축콩단백을 이용하여 대체 소시지, 부분적 대체 소시지 및 닭 소시지의 물리화학적 분석 결과, 대체 소시지가 닭 소시지에 비해 요리 손실량, 튀김 손실량 및 유화성이 유의적으로 낮게 나타났으며(p<0.05), 닭 소시지 보다 물리화학적 품질이 우수하였다. 개발된 소시지의 물성적 특성은 대체 소시지가 닭 소시지에 비해 경도, 응집성, 검성, 씹힘성 및 탄성이 유의하게 높았다(p<0.05). 개발된 소시지의 색도 특성은 대체 소시지의 적색도(a) 및 황색도(b)가 닭 소시지에 비해 유의하게 높았고(p<0.05), 명도(L)는 닭 소시지가 대체 소시지에 비해 유의하게 높았으며(p<0.05), 부분적 대체 소시지는 대체 소시지와 닭 소시지의 사이 특성을 보였다. 따라서 본 연구를 종합해 볼 때, 혼합농축콩단백 소재가 영양학적인 면이 강화되었음을 확인하여 대체육 식품 소재로써의 활용 가능할 것으로 판단된다. 또한, 육류가 조금 들어간 부분적 대체 소시지는 육제품의 섭취를 줄이고 싶은 소비자를 대상으로 하는 대체육 산업에 잠재적인 가능성을 보였으며, 본 연구에서 개발된 대체 소시지는 식품 개발에 기초자료로서 활용 가능성을 보였다. The objective of this study was to optimize the mixing ratio of mixed bean protein concentrate (MBPC) and to improve the quality of sausage analogues. Soybean (Glycine max MERR), mung bean (Phaseolus radiatus L.), red bean [Vigna angularis (Wild.)], and pea (Pisum sativum L.) were mixed and processed to produce a MBPC, which was used to make a sausage analogue. The protein, moisture, and carbohydrate content were significantly (p<0.05) different among the samples. A significant (p<0.05) improvement was observed in textural properties (hardness, gumminess, and chewiness), cooking loss, frying loss, and emulsion stability of the sausage analogue. This study suggested the possibility of attaining high-quality sausage analogues and partial sausage analogues using MBPC, which could serve as a potential ingredient in meat analogues.

음주 여부에 따른 남자 대학생의 건강상태와 식습관에 관한 연구

신경옥,최경순,한경식,최민석,Shin, Kyung-Ok,Choi, Kyung-Soon,Han, Kyoung-Sik,Choi, Min-Seok 한국식품영양학회 2015 韓國食品營養學會誌 Vol.28 No.1

The purpose of this study was to investigate the health status and food habits of male college students in Seoul according to drinking habits. In order to identify risk factors, health surveys were conducted from October 2013 to December 2013. Average height, weight, and BMI of subjects were $175.47{\pm}5.27cm$, $67.93{\pm}9.28kg$, and $22.04{\pm}2.67kg/m^2$, respectively. Weight and BMI were higher in the non-drinking groups compared to the drinking groups (p<0.05). Frequency of drinking was 2~3 times per month, and 52.4% of subjects started drinking when starting university. The motivation to start drinking was cited as "From necessity". Reason for drinking was "Social relations". The favorite kind of drink was beer. Rate of taking supplements was 24.4% of the subjects. Vitamin preparations were highest in drinking groups and non-drinking groups (p<0.05). The reasons for skipping meal were "too busy" in the drinking groups, and "oversleep" in the non-drinking groups (p<0.05). The preference for snacking in drinking groups were milk, milk products, and fast food, with the highest being listed first. The non-drinking groups preferred carbonated drinks and ice cream. The drinking groups need more education about their drinking habits. Drinking groups also need an increased intake of vegetables and fruits.

조도 및 광주기가 명태(Gadus chalcogrammus) 자어의 생존과 성장에 미치는 영향

이기욱(Ki-Wook LEE),한경식(Gyeong-Sik HAN),변순규(Soon-Gyu BYUN),김우진(Woo-Jin KIM),김경덕(Kyoung-Duck KIM),임현정(Hyun-Jeong LIM),김소선(So-Sun KIM) 한국수산해양교육학회 2021 水産海洋敎育硏究 Vol.33 No.4

기간제 근로자의 차별처우금지제도에 관한 고찰

윤기택(Yun Gi-Taek),한경식(Han Kyoung-Sik) 한국법학회 2007 법학연구 Vol.26 No.-

비정규직 고용의 증가는 일면 고용구조의 유연화를 통하여 기업의 국제경쟁력을 강화하고 복잡ㆍ다양한 노동시장의 변화에 신축적으로 대응함으로써 기업의 성장에 기여한 것은 사실이나, 비정규직 근로자들은 임금수준과 기업복지에서 정규직 근로자에 비해 부당한 차별대우를 받아 왔다. 비정규직 근로자에 대한 차별은 사회적ㆍ법적 정의에 반할 뿐만 아니라 노동시장의 건전한 발전을 저해하고 있기 때문에 비정규직 근로자에 대한 차별개선은 노사관계의 양극화를 해소하기 위한 필수요건이다. 비정규직 근로자 중 기간제근로자의 근로조건 보호를 위하여 「기간제 및 단시간근로자보호 등에 관한 법률」이 제정되었으나, 여러 가지 문제점으로 인하여 이에 대한 입법적 개선이 필요하다. The increase of fixed-term employees in the Korean industries has reinforced their competiveness in the international market through its softness and flexibility of the employment structure. Further, it is true that the flexibility has contributed to the positive growth of the industries by enabling to overcome the change of the complicated and various labor market. However, the fixed-term irregular employees have been unreasonably treated when comparing with the regular employees in the wage level and the welfare provided by their companies. The improvement in the treatment of fixed term irregular employees is essential for solving problems caused by the classification and discrimination between two groups, namely, fixed-term irregular employees and regular employees. That's because the discrimination and classification of treatment between two groups is not only contrary to the social justice but also will impede the sound growth of the labour market. The Act on the fixed-term worker and the part-time worker's protection is formulated for the purpose of protecting of the fixed-term worker's labor-condition among the regular worker. but it is require to improve the law due to many problems.

범용 MPU의 PWM 출력을 이용한 PLC 원호보간 기능 구현

이상백(Sang-beak Lee),한경식(Kyoung-sik Han) 대한전기학회 2010 정보 및 제어 심포지엄 논문집 Vol.2010 No.10

이 논문은 PLC 내장 위치결정 기능의 원호보간 기능 구현에 대하여 설명한다. 원호보간 알고리듬은 많은 사람들에 의해 연구되었지만 본 논문에서는 기존에 발표된 비교적 간단한 알고리듬을 창조하여 범용 MPU를 활용한 펄스 제어방법을 소개한다. 특히 원호보간 시 정해진 궤적을 벗어나지 않기 위해서 1㎳ 단위로 새로운 위치 값을 연산하여 운전하는 방식을 채용하였다.

농축 콩단백질 분리 및 추출에 관한 연구

차서희(Seo Hui Cha),신경옥(Kyung-Ok Shin),한경식(Kyoung-Sik Han) 한국식품과학회 2020 한국식품과학회지 Vol.52 No.5

본 연구는 우리나라에서 재배되는 대두, 녹두, 적두 및 완두 4종의 콩 원물을 선발하여 단백을 추출한 후 얻은 농축콩단백의 영양학적 및 기능적 특성을 분석하여 4종 단백 소재의 품질 특성을 알아보고자 하였다. 4종 농축콩단백의 영양학적 특성을 살펴본 결과, 단백질 함량은 72.58-78.69%로 나타났고, 아미노산의 함량은 4종 농축콩단백 모두 aspartic acid, glutamic acid, lysine에서 높은 함량을 나타났으며, 농축녹두단백의 총 필수 아미노산 함량이 3종 농축콩단백에 비해 유의하게 높았다(p<0.05). 4종 농축콩단백의 기능적 특성을 알아본 결과, pH와 겉보기 밀도는 농축녹두단백과 농축적두단백이 다른 농축콩단백에 비해 유의하게 높았고(p<0.05), 수분흡수력은 농축완두단백이, 유지흡수력은 농축대두단백이, 유화 활성은 농축녹두단백이, 유화안전성은 농축녹두단백과 농축완두단백이 다른 농축콩단백에 비해 유의하게 높았다(p<0.05). 따라서 본 연구를 종합해 볼 때, 4종 농축콩단백의 각 기능적 특성을 고려하여 대체육 제품 산업에 적용한다면, 식품소재로서 활용가치가 있을 것으로 판단된다. The purpose of this study was to investigate the nutritional characteristics of selected soybeans grown in Korea to isolate concentrated proteins. Examination of the general characteristics revealed that the pH and apparent density of Phaseolus radiatus L. protein and concentrated Vigna angularis protein were significantly higher than those of other concentrated soy proteins. The water absorption capacity was high for concentrated Pisum sativum L. protein, whereas the oil absorption capacity was high for concentrated Glycine max (L.) Merr. protein. The emulsifying activity and emulsifying stability were higher and significantly higher, respectively, for enriched P. radiatus protein. Considering the functional characteristics of concentrated soy protein, its application as a food material in the meat product industry in Korea may be of potential value.

국내 시판 산양유제품 내 젖소 유성분의 혼입에 대한 조사 연구

정태환 ( Tae Hwan Jung ),전우민 ( Woo Min Jeon ),한경식 ( Kyoung Sik Han ) 한국유가공기술과학회 2013 Journal of Dairy Science and Biotechnology (JMSB) Vol.31 No.2

The aim of this study was to investigate adulteration of caprine milk products by bovine milk using biomolecular techniques with bovine-specific primers for the mitochondrial cytochrome b gene. Polymerase chain reaction (PCR) and real-time PCR assays were applied to caprine milk products including infant formula, city milk, and fermented milk. The results indicated that six out of the eight caprine infant formula products tested contained bovine milk components. In addition, two of the three tested caprine city milk products and two caprine fermented milk products were shown to be adulterated with bovine milk. Conventional PCR results corroborated with results obtained by real-time PCR. This study demonstrates that DNA-based species identification procedures would be useful and applicable in routine examinations of the dairy industry to ensure the quality and safety of dairy foods.