http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Transcriptional Regulatory Role of NELL2 in Preproenkephalin Gene Expression
하창만,김동희,Tae Hwan Lee,Han Rae Kim,최정일,김윤주,강다솔,Jeong Woo Park,Sergio R. Ojeda,Jin Kwon Jeong,Byung Ju Lee 한국분자세포생물학회 2022 Molecules and cells Vol.45 No.8
Preproenkephalin (PPE) is a precursor molecule for multiple endogenous opioid peptides Leu-enkephalin (ENK) and Met-ENK, which are involved in a wide variety of modulatory functions in the nervous system. Despite the functional importance of ENK in the brain, the effect of brain-derived factor(s) on PPE expression is unknown. We report the dual effect of neural epidermal growth factor (EGF)-likelike 2 (NELL2) on PPE gene expression. In cultured NIH3T3 cells, transfection of NELL2 expression vectors induced an inhibition of PPE transcription intracellularly, in parallel with downregulation of protein kinase C signaling pathways and extracellular signal-regulated kinase. Interestingly, these phenomena were reversed when synthetic NELL2 was administered extracellularly. The in vivo disruption of NELL2 synthesis resulted in an increase in PPE mRNA level in the rat brain, suggesting that the inhibitory action of intracellular NELL2 predominates the activation effect of extracellular NELL2 on PPE gene expression in the brain. Biochemical and molecular studies with mutant NELL2 structures further demonstrated the critical role of EGF-like repeat domains in NELL2 for regulation of PPE transcription. These are the first results to reveal the spatio-specific role of NELL2 in the homeostatic regulation of PPE gene expression.
The Molecular Mechanism of NELL2 Movement and Secretion in Hippocampal Progenitor HiB5 Cells
하창만,박재용,황은미,이다용,장성호,이병주,홍성근 한국분자세포생물학회 2013 Molecules and cells Vol.36 No.6
Neural epidermal growth factor-like protein-like 2 (NELL2) is a secreted glycoprotein that is predominantly expressed in the nervous system, but little is known about the intracellular movement and secretion mechanism of this protein. By monitoring the localization and movements of enhanced green fluorescent protein (EGFP)-labeled NELL2 in living cultured hippocampal neuroprogenitor HiB5 cells, we determined the subcellular localization of NELL2 and its intracellular movement and secretion mechanism. C-terminal EGFP-fused NELL2 showed a typical expression pattern of secreted proteins, especially with respect to its localization in the endoplasmic reticulum, Golgi apparatus, and punctate structures. Vesicles containing NELL2 exhibited bidirectional movement in HiB5 cells. The majority of the vesicles (70.1%) moved in an anterograde direction with an average velocity of 0.454 m/s, whereas some vesicles (28.7%) showed retrograde movement with an average velocity of 0.302 m/s. The movement patterns of NELL2 vesicles were dependent upon the presence of microtubules in HiB5 cells. Anterograde movement of NELL2 did not lead to a detectable accumulation of NELL2 in the peripheral region of the cell, indicating that it was secreted into the culture medium. We also showed that the N-terminal 29 amino acids of NELL2 were important for secretion of this protein. Taken together, these results strongly suggest that the N-terminal region of NELL2 determines both the pattern of its intracellular expression and transport of NELL2 vesicles by high-velocity movement. Therefore, NELL2 may affect the cellular activity of cells in a paracrine or autocrine manner.
STANDARDS OUTLOOK 해외 기술표준동향 - 정보보안 분야 전문 인증기관으로 인정
하창인,Ha, Chang-In 기술표준원 2012 기술표준 Vol.123 No.-
사회적으로 보안의 중요성이 강조되면서 보안 인력 양성 또한 시급한 과제로 떠올랐다. 이에 미국표준협회는 카네기멜론대학 소프트웨어공학협회를 보안 분야 공인기관으로 인정하고 보안 전문가 양성에 나섰다.
STANDARDS OUTLOOK 해외 기술표준동향 - ISO 인증, 전 세계 178개국에서 145만여 인증서 활용
하창인,Ha, Chang-In 기술표준원 2012 기술표준 Vol.119 No.-
ISO에서는 2010년도 'ISO 인증 설문조사'에 의거한 자료를 발표했다. 설문조사에 의하면 총 178개 국가에서 145만7,912건의 인증서를 발행하였고, 6.23%의 인증서 활용이 증가한 것으로 나타났다.
하창일,박정일,최석규,이종현,김진형,이도형,Chang Il Ha,Jung Il Park,Suk Kyue Choi,Jong Hyun Lee,Jin Hyoung Kim,Do Hyung Lee 대한안과학회 2008 대한안과학회지 Vol.49 No.11
Purpose: To report three cases of Urrets-Zavalia syndrome after deep lamellar keratoplasty. Case summary: A retrospective chart analysis of two men who underwent deep lamellar keraplasty after corneal chemical burns and one woman who was treated with deep lamellar keratoplasty due to lattice dystrophy was performed. To maintain the anterior chamber depth and prevent formation of a double anterior chamber after deep lamellar keratoplasty, air or gas (C3F8) was injected into the anterior chamber for all three cases. After injections of air or gas (C3F8) into the anterior chamber, pupillary blocks occurred and intraocular pressures increased. Afterwards, intraocular pressures were well-controlled, but the pupil remained irreversibly fixed and dilated despite the use of miotics. Conclusions: Urrets-Zavalia syndrome, a postoperative complication, was first reported in a patient who underwent penetrating keratoplasty for keratoconus. This syndrome can also occur after deep lamellar keratoplasty on rare occasions. J Korean Ophthalmol Soc 2008;49(11):1857-1861
STANDARDS OUTLOOK 해외 기술표준동향 - 새로운 분야 적합성평가 관심
하창인,Ha, Chang-In 기술표준원 2012 기술표준 Vol.120 No.-
CAB과 IEC에서는 신수요 분야의 적합성평가에 적극적으로 나서고 있다. 국내도 이들의 적합성평가를 참고해 적용할 수 있을지 고민해 봐야 할 것이다. 주요 적합성평가에 대해 소개해 본다.
각막확장증 환자에서 펨토초레이저를 이용한 기질내 각막고리 삽입술의 단기 임상 성적
하창일,최석규,이도형,김진형.Chang Il Ha. MD. Suk Kyue Choi. MD. Do Hyung Lee. MD. PhD. Jin Hyoung Kim. MD 대한안과학회 2010 대한안과학회지 Vol.51 No.1
Purpose: To evaluate the clinical results of intrastromal ring segment (KeraringⓇ) implantation using a femtosecond laser in patients with keratectasia. Methods: Twenty eyes of 19 keratectatic patients (15 eyes of keratoconus and five eyes of post-LASIK keratectasia) who completed at least three months of postoperative follow-up were included in this study. Uncorrected visual acuity (UCVA), best spectacle-corrected visual acuity (BSCVA), spherical equivalent, keratometric value, corneal astigmatism, corneal higher order aberrations (HOA) and specular microscopy were determined or performed before and after surgery. In addition, intraoperative and postoperative adverse complications were recorded. Results: UCVA improved in 13 out of 20 eyes (65%), and BSCVA improved in 16 out of 20 eyes (80%). At the postoperative examination there was no statistically significant reduction in the spherical equivalent with that observed at the examination before implantation. Conversely, there were statistically significant reductions in topographic mean K value and Sim K astigmatism, corneal total and coma-like HOA (p<0.05). On specular microscopy, there was no statistically significant difference between preoperative and postoperative endothelial cell density. There was one case of partial Descemet membrane detachment and one case of microperforation as a complication during surgery, which recovered over time without any treatment. Conclusion: KeraringⓇ implantation appears to be an effective and safe procedure for improving visual acuity and stabilizing corneal refractive power in keratectactic patients. J Korean Ophthalmol Soc 2010;51(1):1-7