버섯 종류별 비타민 D 함량 증가 조사

표재성(Jaesung Pyo),구지예(Jiye Gu),김태희(Tae Hee Kim),이정준(Jeong Jun Lee),황미선(Mi Sun Hwang),강재선(Jae Seon Kang),김강민(Kang Min Kim) 한국식품영양과학회 2020 한국식품영양과학회지 Vol.49 No.3

버섯 내의 ergocalciferol, 즉 비타민 D의 함량을 증가시키기 위하여 많은 연구가 진행되고 있다. 본 연구의 목표는 자외선 조사를 통해 버섯을 선정하고 선정된 버섯의 ergocalciferol 함량이 증가한 버섯 제조 조건을 확립하여 제품개발 및 상업화를 위함이다. 본 연구에 사용된 새송이(Pleurotus eryngii), 느타리(Pleurotus ostreatus), 팽이(Flammulina velutipes), 양송이(Agaricus bisporus), 표고(Lentinula edodes) 5종의 버섯을 대상으로 미세분말화한 후 자외선 조사에 따른 ergocalciferol 함량의 증진 효과를 조사하였다. 미세분말화한 버섯은 30분 동안 자외선 조사를 통해 ergocalciferol 함량이 크게 증가하였으며, 그중 표고버섯이 ergocalciferol 함량(76.12±2.53 μg/g)과 전환율(2.98%) 증진에 효과적이었다. 시간별 자외선 조사 시 표고버섯의 ergocalciferol 함량과 전환율이 높은 시간은 35.54 ±1.11 μg/g 및 5.56%로 15분이었다. 지역별 표고버섯의 ergocalciferol 함량이 가장 높은 지역은 함량 99.92±3.71 μg/g 및 전환율 13.45%인 경상도 지역으로 나타났고, ergocalciferol 생성에 관여하는 ergosterol의 함량이 높은 버섯에서 대체로 ergocalciferol 생성이 높았다. Many studies have been conducted to increase the content of ergocalciferol (vitamin D) in mushrooms. This study established the optimal conditions for ergocalciferol-fortified Lentinula edodes. This study investigated the effects of UV-B irradiation with micronized mushrooms for increasing ergocalciferol in five kinds of mushrooms (Pleurotus eryngii, Pleurotus ostreatus, Flammulina velutipes, Agaricus bisporus, and L. edodes). The content of ergocalciferol increased in the mushrooms irradiated to UV-B for 30 minutes. The highest ergocalciferol content (76.12±2.53 μg/g; the conversion of ergosterol to ergocalciferol: 2.98%) was observed in L. edodes irradiated with UV-B. Irradiation of micronized L. edodes for 15 minutes was found to be the optimum period of irradiation in ergocalciferol content (35.54±1.11 μg/g) and conversion (5.56%). L. edodes in Gyeongsangdo, which had been irradiated to UV-B for 15 minutes, also showed the high content (99.92±3.71 μg/g) and conversion (13.45%) to ergocalciferol.

UV-B 조사에 따른 버섯 추출물의 항염증 및 항알레르기 활성

황미선(Mi Sun Hwang),표재성(Jaesung Pyo),김현진(Hyun Jin Kim),도선길(Sun Gil Do),송일대(Il Dae Song),김강민(Kang Min Kim) 한국생명과학회 2022 생명과학회지 Vol.32 No.5

본 연구에서는 표고버섯(Lentinula edodes)을 자외선 조사를 통한 ergocaciferol (비타민 D₂) 함량을 증진시킨 추출물을 이용하여 염증 및 알레르기 반응에 미치는 효과를 확인하였다. 표고버섯 추출물의 항염증 및 항알레르기 효능은 LPS로 활성화된 대식세포(RAW 264.7)와 PMA와 A23187에 의해 활성화된 비만세포(RBL-2H3)로부터 분비 또는 발현되는 TNF-α, IL-6, IL-1β, IL-4와 같은 cytokine과 histamine 분비량을 측정하여 관찰하였다. LPS에 의해 활성화된 대식세포에서 자외선 조사 표고버섯 추출물 처리에 의해 pro-inflammatory cytokine인 TNF-α와 IL-6의 분비량을 ELISA 방법으로 측정하였을 때 현저히 감소함을 확인하였고 mRNA 발현 또한 감소됨을 확인하였다. 비만세포에 자외선 조사 표고버섯 추출물과 PMA, A23187을 함께 처리한 경우 비만세포의 탈과립에 의해 분비되는 histamine의 양이 유의적으로 감소함을 확인하였고 IL-4의 발현양 또한 감소함을 확인할 수 있었다. 이상의 결과는 자외선 조사에 의해 비타민 D₂ 함량을 증진시킨 표고버섯 추출물이 염증과 알레르기 반응의 cytokine의 발현을 저해하는 것으로 보아 염증 및 알레르기 질환의 예방과 치료에 효과적으로 이용될 수 있을 것으로 사료된다. In this study, the effects of UV irradiation-enhanced ergocalciferol (vitamin D₂) content containing Lentinula edodes extract on inflammation and allergic responses were investigated in vitro. The anti-inflammatory and anti-allergic effects of the mushroom extract were tested by estimating the cytokine secretions, such as TNF-α, IL-6, and IL-1β in LPS-activated macrophages (RAW 264.7), or histamine release in PMA and A23187-activated mast cells (RBL-2H3). Under the condition of macrophage activation with LPS, mushroom extract significantly reduced the secretions of pro-inflammatory cytokines, TNF-α and IL-6, and their mRNA expression also matched the observation. The current mushroom extract also significantly reduced the amount of mast cell degranulation-induced histamine secretion from PMA- and A23187-treated mast cells as well as the reduced secretion of IL-4. These results suggest that mushroom extract, which has increased ergocalciferol content by UV irradiation, inhibits the expression of cytokines in inflammation and allergic reactions; therefore, it can be used effectively for the prevention and treatment of inflammatory and allergic diseases.

알코올의존증 치료약물 복용 여부 모니터링을 위한 소변 중 크레아티닌 농도로 보정된 날트렉손 및 6β-날트렉솔 측정값 평가

심영은(Yeong Eun Sim),김선영(Seon Yeong Kim),정재철(Jae Chul Cheong),이현수(Hyun Soo Lee),표재성(Jaesung Pyo),김진영(Jin Young Kim) 대한약학회 2022 약학회지 Vol.66 No.2

This study aimed to detect naltrexone (NTX) and its metabolite 6β-naltrexol (6βNTX) in the urine samples of parolees or probationers who were legally bound to participate in an alcohol dependence treatment program to determine whether to follow the medical treatment ordered by the court. The developed liquid chromatography-tandem mass spectrometry method was suitable for the simultaneous detection of NTX as well as its active metabolite 6βNTX in urine samples. The ratio of creatinine to the analyte ([analyte, ng/mL]/[creatinine, mg/dL]) was used to exclude inaccuracies caused by urine dilution, and this value was further corrected by the average urine creatinine concentration in Korean adults (male 132.6 mg/dL, female 93.3 mg/dL). The cut-off values for NTX and 6βNTX were set at 10 ng/mL. If both the parent drug and its metabolite were found in urine and the corrected creatinine concentrations of NTX and 6βNTX were more than 10 ng/mL, it was considered that the participants were following their treatment protocol accordingly. The results showed that 81 (94.2%, male 69 and female 12) of 86 participants were taking NTX, whereas five (four males, one female) were not adhering to the protocol accordingly. It was found that the four male participants (4.9%) of the 81 participants who were taking the drug initially had urinary NTX and 6βNTX concentrations below the cut-off level without correcting creatinine level, but the values met the acceptance criteria (i.e., >10 ng/mL) of the medical treatment program after correcting the creatinine level. The proposed method can be applied to other drugs in the future.

Determination of Siderophore from Bacillus Mojavensis Using Liquid Chromatography quadrupole Time-of-flight Tandem Mass Spectrometry

Hae In Cheon(천해인),Mi Seon Yeo(여미선),Kang Min Kim(김강민),Jae Seon Kang(강재선),Jaesung Pyo(표재성) 한국생명과학회 2019 생명과학회지 Vol.29 No.2

Bacillus mojavensis를 이용한 항진균성 및 식물 생장 촉진 활성이 최근 보고되었다. 이런 활성은 사이드로포어의 일반적 특성과 일치하여, 본 연구에서는 Bacillus mojavensis가 생산하는 사이드로포어를 고체상추출 카트리지와 액체크로마토그래피-사중극 비행시간형 탠덤 질량분석기를 이용하여 규명하였다. Bacillus mojavensis를 페놀 배지에서 16시간 동안 배양하고 동결 건조 시킨 후, 물에 용해시켜 고상추출 카트리지에 로딩하였다. 카트리지는 5% 메탄올로 세척하고 2% 포름산을 이용해 용출 시켰다. 용출액은 메탄올에 재용해 후 분석을 하였다. Bacillus mojavensis의 사이드로포어는 질량 스펙트럼의 결과를 바탕으로 882.2556의 분자량을 갖는 카테콜타입의 사이드로포어 중 하나인 bacillibactin으로 확인되었다. 이 사이드로포어 분석은 Bacillus 연구 및 기능성 식품 그리고 Bacillus mojavensis의 약학 응용 분야에 큰 기여를 할 것으로 예상된다. Recently, it has been reported that Bacillus mojavensis possesses antifungal properties and plant growthpromoting activities, which are similar to the characteristics of siderophore. In this study, the siderophore produced by B. mojavensis was assessed using a solid phase extraction (SPE) cartridge and liquid chromatography quadrupole time-of-flight tandem mass spectrometry (Q-TOF MS/MS). After B. mojavensis was incubated in phenol medium for 16 hr and lyophilized, the sample was dissolved in water and loaded to an SPE cartridge to remove interferences. The cartridge was washed with 5% methanol in water and eluted with 2% formic acid in methanol sequentially. The eluted solution was evaporated under a stream of nitrogen gas and reconstituted in methanol. The reconstituted sample was filtered, and 1 μl of the sample was assessed using Q-TOF MS/MS. The mass spectrometer was operated using the positive electrospray ionization mode. Based on the mass spectrum and tandem mass spectrum, the siderophore produced by B. mojavensis was bacillibactin, one of the catechol types of siderophore with a molecular weight of 882.2556. This siderophore analysis could provide a justification for the study of B. mojavensis as a functional food and for pharmaceutical applications.