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CNTs 합성을 통해 향상된 비표면적을 갖는 Ti 다공체의 제조
최혜림,변종민,석명진,오승탁,김영도,Choi, Hye Rim,Byun, Jong Min,Suk, Myung-Jin,Oh, Sung-Tag,Kim, Young Do 한국분말야금학회 2016 한국분말재료학회지 (KPMI) Vol.23 No.3
This study is performed to fabricate a Ti porous body by freeze drying process using titanium hydride ($TiH_2$) powder and camphene. Then, the Ti porous body is employed to synthesize carbon nanotubes (CNTs) using thermal catalytic chemical vapor deposition (CCVD) with Fe catalyst and methane ($CH_4$) gas to increase the specific surface area. The synthesized Ti porous body has $100{\mu}M$-sized macropores and $10-30{\mu}m$-sized micropores. The synthesized CNTs have random directions and are entangled with adjacent CNTs. The CNTs have a bamboo-like structure, and their average diameter is about 50 nm. The Fe nano-particles observed at the tip of the CNTs indicate that the tip growth model is applicable. The specific surface area of the CNT-coated Ti porous body is about 20 times larger than that of the raw Ti porous body. These CNT-coated Ti porous bodies are expected to be used as filters or catalyst supports.
Recombinant Human Interleukin-2: II. Biological and Therapeutic Activities
최혜림,윤혜영,함경수,Choi, Hye-Lim,Yun, Hye-Young,Talmadge, James E.,Hahm, Kyung-Soo 생화학분자생물학회 1988 한국생화학회지 Vol.21 No.2
Recombinant human interleukin-2 (rH IL-2, $Ser^{125}$-rH IL-2) purified from E. coli was characterized its biological and therapeutic activities for the establishment of preclinical screening system and therapeutic applications. In vitro augmentation of natural killer (NK) cell activity and mixed lymphocyte reaction (MLR) assay were shown to be comparable with other rH IL-2 from different manufacturers and laboratories. An antiserum against rH IL-2 was obtained from chick, and characterized the reacticvity with rH IL-2 reconstituted from lyophilized state in relation to the stability and conformational changes of the recombinant protein. In addition, long term storage of rH IL-2 in lyophilized or solution was not shown to incur remarkable loss of MLR activities, and any appearance of either degradation or aggregation products when analyzed by SDS-PAGE, indicating the stability and suitability for therapeutic applications of rH IL-2. 대장균으로 부터 분리정제된 재조합 인체 인터루킨-2 (KAIST rH IL-2, $Ser^{125}$-rH IL-2)를 전임상시험 및 암치료에 응용하기 위한 목적으로 생물학적 및 면역요법적 활성도를 측정하였다. In vitro에서 자연살해세포 활성의 증진능력 및 혼합 임파구반응 실험에서 활성을 나타냈으며 다른 회사의 재조합 인터루킨-2 제품들과 유사한 결과를 얻었다. 재조합 인체 인터루킨-2의 입체구조적 및 생물학적 활성도의 안정성을 조사하기 위하여 정제된 재조합 인체 인터루킨-2에 대한 항혈청을 닭에서 얻었으며 동결건조 저장하였던 재조합 인체 인터루킨-2와 이 항체와의 반응을 조사하였으며, 또한 6개월간 저장하였던 인터루킨-2의 안정성을 혼합 임파구반응 및 전기영동 방법으로 그 활성 및 단백질 성분을 조사한바 혼합임파구 반응에서 여전히 활성을 보여주였으며, 전기영동상 분해되었거나 응집된 산물이 생기지 않았음을 확인하였다. 이상의 결과는 KAIST 재조합 인체 인터루킨-2가 전임상시험 및 임상시험 목적에 사용하기에 적합함을 시사한다.
최혜림,하지선,김인식,양승주,Choi, Hye-Rim,Ha, Ji Sun,Kim, In Sik,Yang, Seung-Ju Korean Society for Clinical Laboratory Science 2020 대한임상검사과학회지(KJCLS) Vol.52 No.3
알츠하이머 병은 인지 기능 저하로 인한 치매 발생으로 설명할 수 있는 만성 및 진행성 신경 퇴행성 질환이다. 알츠하이머 병의 특징은 세포 외 및 세포 내 아밀로이드 플라크의 형성이다. 아밀로이드 베타는 알츠하이머 병의 특징이며 미세아교세포는 아밀로이드 베타의 존재하에 활성화될 수 있다. 활성화된 미세아교세포는 전 염증성 사이토카인을 분비한다. 게다가, S100A9는 염증의 중요한 선천성 전 염증 기여자이며 알츠하이머 병에 잠재적인 기여자로 알려져 있다. 이 연구는 아밀로이드 베타 및 S100A9이 처리된 BV-2 세포에서 염증반응 및 NLRP3 인플라마솜 활성화에 대한 메트포르민 및 알파리포산의 효과를 조사했다. 메트포르민과 알파-리포산은 종양 괴사 인자-알파 및 일터루킨-6와 같은 염증성 사이토카인을 약화시킨다. 또한 메트포르민과 알파-리포산은 JNK, ERK, p38의 인산화를 억제하고, NF-kB 경로 및 NLRP3 인플라마솜의 활성화를 억제했다. 또한 메트포르민과 알파-리포산은 M1 표현형인 ICAM1의 수준을 감소시킨 반면 M2 표현형인 ARG1은 증가시켰다. 이러한 발견은 메트포르민과 알파-리포산이 아밀로이드베타 및 S100A9에 의한 신경 염증 반응에 대한 치료제가 될 수 있음을 시사한다. Alzheimer's disease (AD) is a chronic and progressive neurodegenerative disease that can be described by the occurrence of dementia due to a decline in cognitive function. The disease is characterized by the formation of extracellular and intracellular amyloid plaques. Amyloid beta (Aβ) is a hallmark of AD, and microglia can be activated in the presence of Aβ. Activated microglia secrete pro-inflammatory cytokines. Furthermore, S100A9 is an important innate immunity pro-inflammatory contributor in inflammation and a potential contributor to AD. This study examined the effects of metformin and α-LA on the inflammatory response and NLRP3 inflammasome activation in Aβ- and S100A9-induced BV-2 microglial cells. Metformin and α-LA attenuated inflammatory cytokines, such as tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6). In addition, metformin and α-LA inhibited the phosphorylation of JNK, ERK, and p38. They activated the nuclear factor kappa B (NF-κB) pathway and the NOD-like receptor pyrin domain containing 3 (NLRP3) inflammasome. Moreover, metformin and α-LA reduced the marker levels of the M1 phenotype, ICAM1, whereas the M2 phenotype, ARG1, was increased. These findings suggest that metformin and α-LA are therapeutic agents against the Aβ- and S100A9-induced neuroinflammatory responses.
재조합 인체 인터루킨 - II . 생물학적 및 면역요법적 활성도
최혜림,윤혜영,James E . Talmadge,함경수 ( Hye Lim Choi,Hye Young Yun,James E . Talmadge,Kyung Soo Hahm ) 생화학분자생물학회 1988 BMB Reports Vol.21 No.2
Recombinant human interleukin-2 (rH IL-2, Ser^(125)-rH IL-2) purified from E. coli was characterized its biological and therapeutic activities for the establishment of preclinical screening system and therapeutic applications. In vitro augmentation of natural killer (NK) cell activity and mixed lymphocyte reaction (MLR) assay were shown to be comparable with other rH IL-2 from different manufacturers and laboratories. An antiserum against rH IL-2 was obtained from chick, and characterized the reacticvity with rH IL-2 reconstituted from lyophilized state in relation to the stability and conformational changes of the recombinant protein. In addition, long term storage of rH IL-2 in lyophilized or solution was not shown to incur remarkable loss of MLR activities, and any appearance of either degradation or aggregation products when analyzed by SDS-PAGE, indicating the stability and suitability for therapeutic applications of rH IL-2.
ac PDP에서 Addressing 특성개선을 위한 Negative Ramp Slope이 적용된 Reset Pulse에 관한 연구
최혜림,정선욱,강정원,Choi, Hye-Rim,Jung, Sun-Wook,Kang, Jung-Won 한국반도체디스플레이기술학회 2006 반도체디스플레이기술학회지 Vol.5 No.2
A new reset waveform with negative ramp pulse is proposed. Conventional reset waveform applied to the commercial PDP uses a positive ramp pulse. The reset waveforms, especially focused on ramp area, were examined with 2 dimensional fluid code. The proposed negative reset waveform showed much lower ignition voltage ($\sim$70V) as compared with the conventional reset waveform. When the negative ramp pulse was applied, all of the positive-charged ions are collected on the scan electrode. It is found that the ignition voltage of reset discharge due to the negative ramp pulse became lower than that of positive ramp discharge.