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PC12와 BV2 세포에서 동충하초 추출물의 인지능력 개선 효과
최순희,승오탁,이명선,Choi, Soon-Hee,Seung, O-Tak,Lee, Myung-Sun 한국응용과학기술학회 2019 한국응용과학기술학회지 Vol.36 No.2
본 연구는 동충하초 추출물을 PC12 및 BV2 세포에서 인지능력 개선에 대한 효능을 평가하고자 하였다. 동충하초 추출물을 증류수로 추출하여 PC12 및 BV2 세포로 MTT 분석을 통해 세포 생존율을 확인하고 L-glutamate로 유도한 PC12 세포를 통해 세포 보호 효능과 아세틸콜린 함량 및 아세틸콜린에스테아제 활성을 평가하였다. 또한, LPS로 유도한 BV2 세포를 통해 nitric oxide (NO) 및 prostaglandin E2 (PGE2) 생성량 등의 항염증 효능을 측정하고 western blot 분석을 통해 $NK-{\kappa}B$, p38, JNK, caspase-3 등의 단백질 발현량을 확인하였다. 동충하초 추출물은 $200{\mu}g/m{\ell}$ 농도를 제외하고 1, 10, $100({\mu}g/m{\ell})$ 농도에서 세포 독성이 나타나지 않았다. L-glutamate로 유도한 PC12 세포에서 유의성있게 세포 보호 효능과 아세틸콜린 함량의 증가, 아세틸콜린에스테아제 활성 감소가 나타났다. 또한, 동충하초 추출물은 NO 및 PGE2 생성량과 $NK-{\kappa}B$, p38, JNK, caspase-3 등의 단백질 발현을 억제하였다. 이와 같은 결과는 동충하초 추출물이 인지능력에 대한 예방 및 개선 효능이 있음을 나타낸다. 따라서 동충하초 추출물은 인지능력 개선을 위한 새로운 천연 소재로 활용될 수 있다. The aim of this study is to evaluate the efficacy of cordyceps militaris extracts for the improvement of cognitive dysfunction in PC12 and BV2 cells. Cordyceps militaris extracts was prepared by extracting with distilled water. Cell viability was assessed by MTT assay using PC12 cells and BV2 cells. Confirmed effects of L-glutamate induced cytotoxicity test, Acetylcoline (ACh) concentration, and Acetylcolinestase (AChE) activity in PC12 cells. Anti-inflammatory activities of cordyceps militaris extracts was measured through changes in the levels of nitric oxide (NO), and prostaglandin E2 ($PGE_2$) on lipopolysaccharide(LPS)-induced BV2 cell. In addition, we measured the expression of $NF-{\kappa}B$, p38, JNK, and caspase-3 in western blot analysis. Cordyceps militaris extracts showed no cytotoxicity at the concentrations of 1, 10, and $100{\mu}g/m{\ell}$ except for the concentration of $200{\mu}g/m{\ell}$. Cordyceps militaris extracts protected the cell and exhibited significant increases in the ACh concentration and a significant decrease in the AChE activity in L-glutamate induced PC12 cells. Moreover, cordyceps militaris extracts inhibited the productions NO, and PGE2 level and the protein expression of $NF-{\kappa}B$, p38, JNK, caspase-3 in LPS-induced BV2 cells. These results indicate that cordyceps militaris extracts possible prevented and improved cognitive dysfuction symptoms. Thus, cordyceps militaris extracts may be a novel natural material option for the improvement of cognitive dysfunction.
최순희,진구복,Choi, Soon-Hee,Chin, Koo-Bok Korean Society for Food Science of Animal Resource 2009 한국축산식품학회지 Vol.29 No.1
Product quality and shelf-life effect of sodium lactate (SL) in combined with chitosans with various molecular weights (MW) in low-fat sausages (LFSs) stored at $10^{\circ}C$ were evaluated. LFSs with SL and chitosans had 75-76% moisture, 1-2% fat, and 15.8-17.1% protein with a pH range of 6.3-6.6. Water holding capacity was decreased, but most textural properties were increased with the addition of chitosan with MW of 30-40 kDa. Hunter a (redness) values were also increased with the addition of sodium lactate and chitosans in combination with laccaic acid at the level of 0.05%, resulting in similar Hunter a value of 150 ppm of sodium nitrite. The combination of SL and chitosans slightly extended the shelf-life of LFSs approximately 3-6 days at $10^{\circ}C$, resulting in inhibition the growth of L. monocytogenes and E. coli O157:H7, as compared to the control. However, the inhibition of microbial growth at $10^{\circ}C$ was not as strong as that at $4^{\circ}C$. Thus, the storage temperature should be as low ($<4^{\circ}C$) as possible to have a maximum antimicrobial activity in LFS containing SL and various chitosans.
영구염색 시술 후 두피 및 모발 클렌징의 효과에 관한 연구
최순희 ( Soon Hee Choi ),장아람 ( Ah Ram Jang ),이근광 ( Keun Kwang Lee ) 대한미용문화예술학회 2012 대한미용문화예술학회지 Vol.1 No.1
The result of cleansing hairs using the product after permanent hair dye is given as follows. We can see that contaminations are removed by using white scalp materials and hair dye, rather than by using usual detergents. The results of measuring a color degree using a color meter step in terms of scalp and hairs before and after dye using the product are given as follows. The color degree, L* value, is 43.797, and red and yellow are included very much. However, after dyeing, the color degree of the scalp, L* value, is 29.925; the color of the scalp becomes dark; red is reduced. When we measured the color degree using the scalp cleansing product, the L* value is 37.198; the same color pattern is shown. The scalp has its bright color and is clean; especially the red portion is increased to recover it to its original form. However, there are little different color, comparing with the color before the dye (L* 17.693); the scalp cleansing product increase the brightness (L* 18.144); especially the portion of red is increased. Therefore, the collected results show that after the permanent dye, coloring hair chemicals are not removed after washing, but the specialized scalp cleansing product removes effectively the coloring hair chemicals and remaining.
기본 심폐소생술 교육이 초·중등 교사의 심폐소생술에 대한 지식, 태도 및 자기효능감에 미치는 효과
최순희(Choi, Soon-Hee) 한국보건간호학회 2015 韓國保健看護學會誌 Vol.29 No.1
Purpose: This study was conducted to evaluate the effect of CPR education on the knowledge, attitude and self-efficacy of elementary and middle school teachers. Methods: After obtaining informed consent from participants, data were collected from 75 teachers receiving training in emergency care using questionnaires before and after education. Data were analyzed using percentage, paired t-test, and correlation. Results: The knowledge and self-efficacy scores were the middle level and the scores for attitude were the upper middle level. The knowledge, attitude, and self-efficacy scores at posttest were significantly higher than those at pretest. Conclusion: Teachers should receive CPR education regularly, so that they can acquire the knowledge, attitude, and practical ability of CPR.
음주예방 프로그램이 여고생의 음주관련 지식과 태도, 음주거절 자기효능 및 음주행위에 미치는 효과
최순희(Choi Soon Hee),박민정(Park Min Jung) 기본간호학회 2008 기본간호학회지 Vol.15 No.2
Purpose: This study was done to determine the effect of drinking prevention program on the levels of drinking related knowledge, attitude, drinking refusal self-efficacy and behavior in high school girls. Method: This was nonequivalent control group pre-post test design. The subjects were 133 high school girls who were conveniently assigned to experimental and control group. The data were collected from May to September, 2005 and analyzed using Chi-square test, t-test and ANCOVA. Results: 1. The 1st hypothesis, "The drinking-related knowledge score of experimental group will be significantly higher than that of control group in posttest" was supported (F=256.30, p=.001). 2. The 2nd hypothesis, "The drinking-related attitude score of experimental group will be significantly higher than that of control group in posttest" was supported (F=43.35, p=.001). The 3rd hypothesis, "The drinking refusal self-efficacy of experimental group will be significantly higher than that of control group in posttest" was supported (F=43.86, p=.001). 4. The 4th hypothesis, "The number of drinking of experimental group will be significantly lower than that of control group in posttest" was supported (F=6.12, p=.015). 5. The 5th hypothesis, "The drinking amount of experimental group will be significantly lower than that of control group in posttest" was supported (F=8.52, p=.004). 5. Conclusion: This drinking prevention program was effective in increasing the drinking-related knowledge and attitude, and the drinking refusal self-efficacy, and in reducing the drinking behavior of high school girls.