Solubilization of Brain Phospholipase D by Taurodeoxycholate: Activational Effect of Some Matal Ions

최석우,최명언,Choi, Seok Woo,Choi, Myung Un Korean Chemical Society 1997 대한화학회지 Vol.41 No.12

쥐의 미크로좀 포스포리파제 D(PLD)를 센 이온세기 상태에서 0.2% taurodeoxycholate를 사용하여 용해시켰다. 포스포리파제 D의 활성은 기질로 방사성 동위원소로 표지 된 dipalmitoylphophatidylcholine을 사용하여 생성된 phosphatidic acid(PA)를 측정하여 결정하였다. 용해된 PLD의 초적 pH와 온도는 각각 6.5와 30$^{\circ}C$로서 용해되기 전 미크로좀 상태의 PLD와 비슷하였다. 올레산의 활성화 효과는 4 mM 농도에서 관찰되었다. PLD 활성도에 미치는 금속이온 영향을 조사한 결과 $Mg^{2+},\; Ca^{2+},\; Sr^{2+}, \;Ba^{2+}$와 같은 알칼리 토금속은 모두 PA 생성을 촉진시킨 반면, $Cu^{2+},\; Cd^{2+},\; Al^{3+},\; Ni^{2+},\; V^{5+}$는 억제하였다. Microsomal phospholipase D (PLD) in rat brain was solubilized employing 0.2 % taurodeoxycholate in high ionic strength. Phopholipase D activity was determined by measuring product phophatidic acid (PA) using isotope-labelled dipalmitoylphophatidylcholine as a substrate. The solubilized PLD showed an optimal pH of 6.5 and the highest activity at 30$^{\circ}C.$ These properties were similar to those of microsomal PLD before solubilization. The stimulatory effect of oleic acid was observed at the concentration of 4 mM. When effects of metal ions on PLD activity were examined, alkaline earth metals such as $Mg^{2+},\; Ca^{2+},\; Sr^{2+}, \;Ba^{2+}$ promoted the PA production but $Cu^{2+},\; Cd^{2+},\; Al^{3+},\; Ni^{2+},\; V^{5+}$ showed inhibitory effects.

봉약침과 홍화약침으로 치료한 레이노 현상을 동반한 수근관증후군 환자 1례

최석우,박평범,오성종,Choi, Seok-Woo,Park, Pyeong-Beom,Oh, Sung-Jong 대한약침학회 2009 Journal of pharmacopuncture Vol.12 No.1

Objectives : This study is to report the effect of Pharmacopuncture therapy on a patient suffering from the pain and cold intolerance of hand caused by Carpal tunnel syndrome with Raynaud's phenomenon. Methods : We had treated the patient diagnosed as Carpal tunnel syndrome with Raynaud's phenomenon by Sweet BV and CF pharmacopuncture. We injected Sweet BV and CF into acupuncture points on both hands - Sweet BV into Baxie (EX-UE9), CF into Naegwan ($PC_6$) and Daereung ($PC_7$). And then we evaluated her symptoms by VAS (Visual Analog Scale). Results : Clinical symptoms about Carpal tunnel syndrome with Raynaud's phenomenon were remarkably improved by Sweet BV and CF Pharmacopuncture. Conclusion : Therefore, we concluded that pharmacopuncture therapy - Sweet BV, CF etc. - may be useful to treat Carpal tunnel syndrome with Raynaud's phenomenon.

오공약침으로 치료한 슬와부 통증 환자 2례

최석우,배진승,김영주,Choi, Seok-Woo,Bae, Jin-Seung,Kim, Young-Joo 대한약침학회 2007 Journal of pharmacopuncture Vol.10 No.1

Objectives : This study is to report the effect of Scolopendrid Pharmacopuncture on two patients suffering from the pain of popliteal part. Methods : We treated the patients with the pain of popliteal part by acupuncture according to meridian(Taeyang and Soyang-Meridian), and we injected 0.1${\sim}$0.2cc of Scolopendrid Pharmacopuncture to his pain site. And then we evaluated their pain by VAS(Visual Analog Scale). Results : 1. A combined treatment-Scolopendrid Pharmacopuncture and Acupucture- had a very excellent effect on relieving pain of popliteal part originated from trigger point and referred pain of Popliteus or Biceps Femoris. 2. It appears that Scolopendrid Pharmacopuncture is very effective against trigger point of muscle and pain site fixed.

황련해독탕 약침액으로 희석한 BU의 신체반응에 관한 연구

최석우,노정두,설현,소웅룡,육태한,Choi, Seok-woo,Rho, Jung-doo,Seol, Hyun,So, Woong-ryong,Yook, Tae-han 대한침구의학회 2003 대한침구의학회지 Vol.20 No.5

Objective : In the pain control, BU herbal acupuncture is highly effective but causes many phys ical reactions. This study was done to compare BU with BU mixed Hwangryunhaedoktang(HHT, 黃連解毒湯) herbal acupucture in decreasing physical reactions of BU. Methods : We injected BU Group(N=12) and BU mixed HHT Group(N=12) at four points of Fenmen(B12), Feishu(B13), Fufen(B41) and Pohu(B42). Then we observe the local thermal changes and the physical reactions at beginning, 1hour and 24 hours after administering herbal acupuncture through the D.I.T.I. and survey. Results : The following results were obtained; 1. The significant dermatothermal difference had been checked 1hour and 24hours in BU group, but 1hour in Bu mixed HHT. 2. In BU group, the difference of temperature had been continued highly until 24hours. But in BU mixed group, the difference of temperature had been continued highly until 1 hour but decreased nearly equal to beginning stage until 24hours. 3. The symptoms which appear following the herbal acupuncture administration were less appeared in BU mixed HHT than BU group.

마황천오약침의 비만치료응용에 대한 연구

최석우,배진승,김영주,Choi, Seok-Woo,Bae, Jin-Seung,Kim, Young-Joo 대한약침학회 2006 Journal of pharmacopuncture Vol.9 No.3

Objectives : This study is to research if any acupucture points are more effective, when Mahuang-chuanwu herbal acupucture is applied to the theatment of obesity. Methods : We searched oriental medical literatures and papers. Results : 1. Mahuang-chuanwu Herbal Acupuncture consists of distilled water of Ma-hunag and Chuan-wu, and it has the following effects : channel-warming, cold-dispering and eliminating damp. therefore it is applied to the treatment of obesity. 2. The acupuncture points, which have effects of channel-warming, cold-dispering and eliminating damp, are six : Kwanwon(Cv4), Kihae(CV6), Changmun(Liv13), Chok-samni(St36), Chung-wan(Cv12), Chonchu(St25). And Kwanwon(Cv4), Kihae(CV6), Changmun(Liv13), Chung-wan(Cv12), Chonchu(St25) locate on the abdomen. 3. Morbidly obese people, especially those in which their bellies are very large, are so because often the binding properties of the Taemaek(G26) have weakend. therefore Taemaek(G26) will be more effecive than Changmun(Liv13) that locate on right below the free end of the 11th rib, at the lateral side of the chest. 4. Mahuang-chuanwu Herbal Acupuncture will be most effective on the treatment of obesity with cold and damp as additional treatment, when it is injected into Kwanwon(Cv4), Kihae(CV6), Chonchu(St25), Taemaek(G26).

Sweet Bee Venom 시술후 발생되는 소양감에 대한 임상보고

최석우,최세운,오성종,Choi, Seok-Woo,Choi, Se-Un,Oh, Sung-Jong 대한약침학회 2010 Journal of pharmacopuncture Vol.13 No.3

Objectives : This study is to report the percentage of localized itching which occurred, when we injected to patients with Sweet Bee Venom(Sweet BV). Methods : We investigated 374 patients who had injected with Sweet BV in our clinic from February 15. 2009 to April 30, 2010. We checked the number and percentage of patients who occured localized itching on injection area. Then we analyzed those according to times in treatment, the body parts of injection and treatment dosage. Results and Conclusion : Localized itching was lower by 1.60% in the first treatment with Sweet BV. However localized itching was 12.83% in the whole course of treatment, which showed a similar incidence of 13% in Bee Venom. Therefore it can be interpreted that Sweet BV may help suppress the immune responses such as itching in the initial treatment, but the occurrence of local immune responses of Sweet BV may be similar to that of Bee Venom in continued treatment. We suppose that we should be careful of the occurrence of local immune responses as Bee Venom at least until the fourth treatment in clinical application with Sweet BV, although localized itching did not occur in the first treatment. Also we should be careful of treatment with Sweet BV in body parts, such as wrist, hand, chest and abdominal, because the percentage of localized itching was relatively high in those parts.

구안와사 호전요인에 관한 연구

최석우,육태한,송범룡,Choi, Seok-woo,Yook, Tae-han,Song, Beom-yong 대한침구의학회 2004 대한침구의학회지 Vol.21 No.3

Objective : In Modern society, patients with facial nerve paralysis are increasing because of many factors - irregular life, cold, overwork and stress etc. We have troubles in presuming the prognosis, though how to diagnosis and examine facial nerve paralysis are many. Methods : A clinical study was done on 89 patients who were diagnosed and treated as facial nerve paralysis(Bell's palsy) from January 2001 to May 2003 at the Dep. of Acupuncture and Moxibustion, college of Oriental Medicine, Woo-suk University. We classified 89 patients as the Sasang(四象) constitution, contributing factor, season, age and existence of diabetes items and analyzed, as we would research differences of changing point-Period from on set of Bell's palsy to the day which the change begins to be seen at the face- and improvement -Period which Bell's palsy is improved from on set to H-B grade II. Results : 1. Among the Sasang constitution, Soeumin($10.67{\pm}3.77days$) were faster than other constitutions, but Soyangin($16.25{\pm}6.75days$) were slower than other constitutions in changing point. Taeumin($4.12{\pm}1.49weeks$) were shorter than other constitutions, but Soyangin($4.88{\pm}2.11weeks$) were longer than other constitutions in improvement. 2. Among contributing factors, overwork and stress group were slower than other contributing factors in changing point($13.95{\pm}6.52days$), and longer than others in improvement($4.67{\pm}1.87weeks$). 3. Changing point and improvement of season, age, and existence of diabetes had the difference of the average according to an each item, but they were not statistically significant. 4. In 89 patients with Bell's palsy, average changing-point was $13.11{\pm}5.99$(days) and average improvement was $4.47{\pm}1.82$(weeks). Their correlation was 0.687 and statistically significance(P<0.01), therefore we could decide that their relation is highly correlation.

오공약침으로 치료한 고관절 골절수술 후 발생된 내전근통증 환자 1례

최석우,임성택,신진철,육태한,Choi, Seok-Woo,Lim, Sung-Taek,Shin, Jin-Cheol,Yuk, Tae-Han 대한약침학회 2006 Journal of pharmacopuncture Vol.9 No.1

Objective : This study is to report the effect of Scolopendrid Herbal Acupuncture on a patient suffering from the pain of Hip Adductors after operation for hip fracture. Methods : First we had treated the patient with the pain of Hip Adductors by acupuncture and herb medication(活絡湯), but we did't get remarkable improvement in condition of patient. So we added to treat his pain site with Scolopendrid Herbal Acupuncture. And then we evaluated his pain by VAS(Visual Analog Scale). Results : 1. Scolopendrid Herbal Acupuncture had a very excellent effect on relieving pain and inflammation of Hip Adductors after operation for hip fracture. 2. It appears that Scolopendrid Herbal Acupuncture is very effective against Trigger Point of muscle and pain site fixed.

Ethynyl 말단기를 갖는 Polyisoimide 올리고머의 합성 및 이들의 경화거동에 관한 연구

최석우(Seok Woo Choi),김보옥(Bo Ock Kim),김지홍(Ji Heung Kim),남성우(Sung Woo Nam),전붕수(Boong Soo Jeon),김영준(Young Jun Kim) 한국고분자학회 2014 폴리머 Vol.38 No.6

4-ethynylaniline(4-EA) 혹은 4-phenylethynyl phthalic anhydride(4-PEPA)을 end-capper로 사용하여 분자량이2500 g/mol 혹은 5000 g/mol로 조절되고, 반응성 ethynyl 기를 갖는 4,4``-diamino diphenyl ether(4,4``-ODA)/3,3``,4,4``-benzophenone tetracarboxylic acid dianhydride(BTDA), 4,4``-ODA/4,4``-oxydiphthalic anhydride(ODPA)계 폴리아이소이미드 올리고머를 합성하여 이들의 중합 및 경화거동을 연구하였다. FTIR 분광분석을 통하여 이들 폴리아이소이미드 올리고머 말단에 반응성 ethynyl 기가 포함되어 있음을 확인할 수 있었다. 이미드화 반응은 폴리아이소이미드올리고머의 화학구조에도 영향을 받을 뿐 아니라 올리고머의 분자량에도 영향을 받아 분자량 2500 g/mol 올리고머가 5000 g/mol 올리고머보다 대략 10 oC 정도 낮은 온도에서 이미드화 반응이 일어났다. Ethynyl기의 가교반응은 이미드화 반응보다 높은 온도에서 일어났으며 4-EA로 end-cap된 폴리아이소이미드 올리고머에 대해서는 이 두 반응이 DSC 그래프에서 겹쳐서 나타났다. 4-PEPA로 end-cap된 4,4``-ODA/ODPA 폴리아이소이미드 올리고머에 대해 등속도 가열 DSC 실험을 통해 이미드화 반응 및 가교반응에 대한 활성화 에너지와 지수앞인자를 구하였다. 이미드화반응에 대한 활성화 에너지는 141 kJ/mol, 지수앞인자 값은 1.45×1013 min-1, 가교반응에 대한 활성화 에너지는177 kJ/mol, 지수앞인자 값은 2.90×1013 min-1이 얻어졌다. Acetylenic or phenylethynyl end-capped polyisoimide oligomers (Mw 2500 g/mol, 5000 g/mol) based upon4,4``-diamino diphenyl ether (4,4``-ODA)/4,4``-oxydiphthalic anhydride (ODPA) and 4,4``-ODA/3,3``,4,4``-benzophenone tetracarboxylicacid dianhydride (BTDA) were synthesized by using 4-ethynylaniline (4-EA) or 4-phenylethynyl phthalicanhydride (4-PEPA) as an end capper. The incorporation of ethynyl groups were confirmed by FTIR spectroscopy. Theisomerization temperature was influenced by molecular weight as well as the backbone structure of polyisoimides oligomers. Thus, polyisoimide oligomers with molecular weight of 2500 g/mol was found to be imidized at temperature10 oC lower than that for the oligomers with molecular weight of 5000 g/mol. The cross-linking reaction of ethynylgroups occurred at a higher temperature than that for the isoimide/imide isomerization reaction. These two reactions weretotally or partially overlapped on the DSC thermograms for the polyisoimide oligomer end-capped with 4-EA. Kineticsof thermal imidization and cross-linking reactions for the 4,4``-ODA/ODPA polyisoimide oligomers end-capped with 4-PEPA were investigated by performing dynamic DSC experiments at heating rate of 10 oC/min. The activation energyand pre-exponential factors were 141 kJ/mol and 1.45×1013 min-1 for the imidization reaction and 177 kJ/mol and2.90×1013 min-1 for the cross-linking reaction, respectively.

국내 화재조사체계의 개선방안에 관한 연구

최석우(Choi Seok woo),최지훈(Choi Ji Hun),최돈묵(Choi Don mook) 한국방재학회 2017 한국방재학회논문집 Vol.17 No.5

제조물책임법 및 실화 책임에 관한 법률 개정, 화재감식평가기사 자격제도의 시행 등 화재분야의 변화는 현재 화재감식체계의 개선을 필요로 한다. 따라서 본 연구에서는 국내외 화재조사체계를 비교·분석하여 문제점 및 개선방안을 제시하려고 한다. 화재조사교육훈련의 실무교육 확대와, 전담인력의 보강, 전문인력 확충하여 화재조사 전문인력을 보강한다. 전문보직관리제시행과 화재조사 자격증소지자에 대한 인사상 우대 및 인센티브를 부여하여 자격증제도와 업무와의 연계를 도모하고, 화재감식 평가기사 상위 등급 자격제 도입으로 자격별 역할을 분할한다. 화재조사에 관한 법률을 개정하여 소방의 화재조사 권한 강화 및 소방·경찰간 협조체계를 구축한다. Improvement of fire identification system is necessary due to the Product Liability Law and Accidental Fire Liability Act. Therefore, the present study scrutinizes South Korean fire identification system, compares with foreign fire identification system to find out problems and present ways to improve the domestic fire identification system. expand practical training in the fire investigation education and training programs. reinforce staff dedicated to fire identification in the dimension of labor division in the firefighting sector. Expand the pool of experts. Implement special position management system and grant advantage in personal management. Introduce a higher level certification scheme of fire identification and estimation engineer and allocate work. amend laws to strengthen the fire investigation authority of the firefighting unit and manage specific matters in the cooperative structure between firefighting and police.