Electromyographic changes in masseter and sternocleidomastoid muscles can be applied to diagnose of temporomandibular disorders: An observational study

최광호,O Sang Kwon,Lak-Hyung Kim,So Min Lee,정의민,정지연 한국한의학연구원 2021 Integrative Medicine Research Vol.10 No.4

Background The diagnosis of temporomandibular disorders (TMDs) is an important part of the functional cerebrospinal technique (FCST). In addition, surface electromyography (sEMG) is an important candidate for diagnosing TMD. In FCST, despite the importance of the cranio-cervical-mandibular system, few sEMG parameters consider TMDs. Thus, this study evaluated the possibility of TMD diagnosis by sEMG. Methods The study was conducted as an assessor-blinded cross-sectional study. Each of 35 participants were recruited for patient group and normal group separately based on the Diagnostic Criteria for TMD Symptoms Questionnaire (DC/TMD SQ). The sEMG was measured by attaching electrodes to sternocleidomastoid muscles (SCMM) and masseter muscles (MM) before and after wearing the temporomandibular joint balance appliance (TBA). Results The percentage overlapping coefficient (POC) value of the healthy control group was increased compared with the TMD group. Receiver operating characteristic (ROC) analysis revealed that the area under the curve (AUC) value of the SCMM was greater than that of the MM. POC values before and after the SCMM also revealed significant changes compared to the MM. Conclusion This study showed that the sEMG measurement of the SCMM is useful for TMD diagnosis in traditional Korean medicine.

누에 견사선에서 분리한 RNA binding protein-1 유전자 프로모터 분석

최광호 ( Kwang Ho Choi ),김성렬 ( Seong Ryul Kim ),김성완 ( Sung Wan Kim ),구태원 ( Tae Won Goo ),강석우 ( Seok Woo Kang ),박승원 ( Seoung Won Park ) 한국잠사학회 2014 한국잠사곤충학회지 Vol.52 No.1

효율적인 형질전환 누에 시스템 구축을 위해서는 새로운 전이인자의 개발과 함께 선발을 위한 마커 유전자 및 transposase 발현을 효과적으로 조절할 수 있는 다양한 유전자 프로모터 개발이 필수적이다. 이와 관련하여 선행연구를 통해 누에 후부실샘으로부터 고발현하는 RNA binding protein-1 homologue(RBP-1) 유전자를 선발한 바 있다. 본 연구에서는 RBP-1유전자의 누에 발육시기별 및 유충 조직별 발현양상을 Northen blot hybridization 방법으로 분석한 결과, RBP-1 유전자는 유충기로부터 번데기 후기까지의 전기간에 걸쳐 발현하였으며, 두부, 표피, 중장, 지방체 및 견사선 등 실험한 모든 유충 조직에서 고발현하는 것으로 관찰되었다. 또한, 누에 게놈 유전자은행을 제작한 후 RBP-1 cDNA 유전자를 탐침으로 5``-UTR 영역을 클로닝하고 luciferase assay 방법으로 RBP-1 유전자 프로모터의 활성을 분석하였다. 실험 결과, RBP-1 cDNA를 탐침으로 RBP-1 유전자 ORF와 5’-UTR이 포함된 약 1,660 bp 영역의 게놈 유전자를 클로닝하였다. RBP-1 유전자 프로모터 활성검정을 위해 전사 개시점(+ 30)으로부터 상류의 -740 bp 영역을 PCR로 분리한 후 pGL3 basic vector에 도입하여 luciferase 활성 측정을 위한 전이벡터, pGL-RBP1를 제작하였다. 제작된 pGL-RBP1는 곤충 세포주(Sf9)에 transfection 한 후 luciferase 발현량을 측정한 결과, 기존의 BmA3 유전자 프로모터 대비 10% 가량 높은 발현 효율을 확인할 수 있었다. We isolated highly-expressed genes in the posterior silk glands of silkworm on a previously study, which one of these was identified as RNA binding protein-1 homologue (RBP-1) gene. In this study, we investigated gene expressional characteristics of the RBP-1 depending on silkworm development stages and several tissues of the larvae, respectively. Northern blot hybridization analysis showed that the RBP-1 gene was expressed high in larval and pupal periods, and highly expressed than endogenous internal control gene (BmA3) on all tested larval tissues. In addition, we isolated and analyzed a phage DNA having 1,660 bp-long promoter region of the RBP-1 gene from a genomic DNA library. To study the RBP-1 gene promoter activity, RBP-1 (?740/+ 30) was amplified by PCR and subcloned into a pGL3 basic vector to generate pGL-RBP1. A luciferase report vector carrying RBP-1 gene promoter (770 bp) was tested by luciferase assay in Sf9 cells. In the result, the RBP-1 gene promoter was more efficient than constitutive promoter (BmA3) by approximately ten percent.

누에 중장유래 생체방어 관련 유전자 개발 연구

최광호 ( Kwang Ho Choi ),구태원 ( Tae Won Goo ),김성렬 ( Seong Ryul Kim ),박승원 ( Seung Won Park ),김성완 ( Sung Wan Kim ),강석우 ( Seok Woo Kang ) 한국잠사학회 2012 한국잠사곤충학회지 Vol.50 No.2

This study was aimed for identification of a useful genetic resources from the entomopathogenic bacteria infected-midgut of the silkworm, Bombyx mori L. We analyzed the appropriately midgut-immunizing condition of 4th instar larvae by a feeding infection using several entomopathogenic bacteria. Xenorhabdus nematophila was selected as a suitable bacteria for midgut immunization of Jam 123, B. mori. We constructed a subtraction cDNA library from the mRNA of the immunized midgut, respectively. A total of 1,000 clones were randomly selected from the subtracted cDNA library, and then performed a differential display hybridization analysis with forward and reverse probes. In conclusion, nine clones were identified as differential expressed genes, which presumed that these genes were involved in gut immunity of silkworm. The total number of clones analyzed in this work is not enough to have a brief overview of a understanding on the midgut immunity factors of silkworm. Therefore, further defined studies on these molecules biological roles will give us well-fined information about the innate immune mechanism of silkworm.

누에나방 수명관련 특이발현 유전자 탐색

최광호 ( Kwang Ho Choi ),구태원 ( Tae Won Goo ),김성렬 ( Seong Ryul Kim ),김성완 ( Sung Wan Kim ),강석우 ( Seok Woo Kang ),강필돈 ( Pil Don Kang ) 한국잠사학회 2013 한국잠사곤충학회지 Vol.51 No.2

In general, the mean silkmoth lifespan is around 8 days for female and 5 days for male. But, the duration of J037 strain`s lifespan is remarkably long in both sexes. On the contrary, the Daizo (sdi) strain has a remarkably short lifetime. The differences in adult lifetime among various silkworm strains has been suggested that the adult lifetime may be genetically controlled. In this experiment, using J037 and Daizo strains we investigated genetic factors related to the adult lifetime of silkworm. We constructed the full-length cDNA library from the adult male of the J037 strain. A total of 2,688 clones were randomly selected, and we performed a differential display hybridization with cDNA probes generated from J037 and Daizo adult males. In conclusion, 193 clones were identified as differential expressed genes, and 154 unique genes were generated after the assembly of 193 clones. Of the 154 unique genes, the most abundant genes were cytochrome oxidase subunit-1 gene (9 times) and unknown (clone ID; 1-50) gene (5 times). The functional groups of these unique genes with matches in the AmiGo database were constructed according to their putative molecular functions. Among thirteen functional categories, the largest group was unclassified protein (24%). In addition, we analyzed the nucleotide and deduced amino acid sequences of the most highly occurred gene (1-50, EF434397), which consisted of 240 amino acids. However, it is confirmed yet that these genes really have an affected on the silkworms longevity. Further studies on these molecules biological roles will give us well-fined information about mechanisms of insect aging and/or scenesence.

Kanamycin 과 Talampicillin 병용요법의 단순 남성임질 치료효과

최광호(Kwang Ho Choi),양인섭(In Sub Yang),김재홍(Jae Hong Kim),김중환(Joong Hwan Kim) 대한피부과학회 1985 大韓皮膚科學會誌 Vol.23 No.4

The treatment of gonorrhea caused by beta-strains of N. gonorrhoeae is quite different from that of non-beta-strains, but it is not always possible in general practice to take cultures of gonococci to detect p-lactamase producing organisms. Therefore treatment regimens commonly used for gonorrhoea should be effective against both PPNG non-PPNG strains. Eighty-seven male uncomplicated gonorrheal patients were treated with intramuscular kanarnycin 2gm in combilnation with talampicillin 2. 5 gm plus probenecid 1grn orally. Sixty-seven patients were followed up. All patients recovered. Ten patients among sixty-seven patients had PPNG. A regimen of intramuscular kanamycin 2 gm, talampicillin 2. 5 gm plus probenecid 1 0 gm orally seems to be one of the most cost effective regimens in Korea.

인두임질에 관한 임상적 연구

최광호(Kwang Ho Choi),김재홍(Jae Hong Kim),김중환(Joong Hwan Kim) 대한피부과학회 1985 大韓皮膚科學會誌 Vol.23 No.3

Five hundred seventeen male patients with gonococcal urethritis at the VD clinic of Chocng-ku Public Health Center between Feb. 27 and Oct. 27, 1984 the were source cf this study. Forty-five of seventynine patients who had practiced cunnilingus were actual subjects of this study. Aeiss.ria gonorrhoeae were cultured from the pharynx of five patients: one was found to be PPNG. All 5 pharyngeal gonorrhea patients were asymptornatic and their throat appeared to be normal, except injection of the pharynx in one patient. Two patients infected by non-PPNG were administered an oral dose of 1. 0 gm probenecid plus 2 5 gm talampicillin and 2. Ogm Kanamycin sulfate, IM and one patient infected by non-PPNG was administred an oral dose of l. Ogm probenecid and, 3i) minutes later, 6. 0 m.u. fortified procaine penicillin G IM. One gatient infected by PPNG was administered an oral dose of 1, 0 gm probenecid and 30 minutes later, 1 vial of sulbactam sodium/ampicillin sodium, IM. All four pharyngeal gonorrgeal patients were cured, One patient was lost from further evaluation, We consider it important to have pharyngeal cultures done on all gonorrheal patients, at least on those who admit having had orogenital contact in recent episode, because pharyngeal gonococci may be the source of disseminated gonococcal disease and in rare circumstances, the source of mfection for sexual partners, and single lose spectinomycin and orally administered penciillin regimens that are effective against anogenital gonorrhea, had been known to have high rates of failure when used in the pharyngeal gonrorhea.

누에 수정란 초기발현유전자 데이터베이스 구축

최광호 ( Kwang Ho Choi ),구태원 ( Tae Won Goo ),김성렬 ( Seong Ryul Kim ),김성완 ( Sung Wan Kim ),전재범 ( Jae Buhm Chun ),박승원 ( Seoung Won Park ),강석우 ( Seok Woo Kang ) 한국잠사학회 2013 한국잠사곤충학회지 Vol.51 No.2

This study was aimed for development of a useful genes that has a transcript expressional specificity in the early embryonic stage of the silkworm, Bombyx mori. We constructed and analyzed a full-length cDNA library from silkworm`s eggs which after a lapse of 2 ~ 6 hours post oviposit. A total 960 clones were randomly selected, and the 5` ends of the inserts were sequenced to generate 652 expressed sequence tags (EST). 334 unique ESTs were generated after the assembly of 652 ESTs. The annotation of 334 unique ESTs by BLAST search revealed that 156 (47%) of the sequences represented known genes, whereas 178 (53%) of the sequences has no matches in the database. Of the 156 known genes, the most abundant genes were heat shock protein hsp20.8 gene (12 times) and ubiqutin-like protein gene (11 times). The functional groups of these ESTs with matches in the database were constructed according to their putative molecular functions. Among thirteen functional categories, the largest groups were protein synthesis (9.6%) and cellular organization (8.1%). Further defined studies on molecular functions and biological roles of their promoters will give us well-fined information and its application.

누에 피브로인 유전자 다형성 마커 개발

최광호 ( Kwang Ho Choi ),김성렬 ( Seong Ryul Kim ),강석우 ( Seok Woo Kang ),박옥란 ( Yulan Piao ),김성완 ( Sung Wan Kim ),김기영 ( Kee Young Kim ) 한국잠사학회 2015 한국잠사곤충학회지 Vol.53 No.2

본 실험에서는 누에 피브로인 L-chain 유전자의 인트론 영역을 사용하여 우리나라에서 보존하고 있는 누에 계통중 원산지별 누에 계통 8개를 대상으로 계통 간 유전자 다형성을 비교하였다. 실험 결과, 실험에 사용한 누에 계통에 따라서 동일한 계통 내 모든 개체에서 FibL1과 FibL2의 유전자다형성 조합이 일정하게 유지되어 있었으며, 계통 간에는 FibL1과 FibL2의 유전자 다형성 패턴 조합이 다소 상이한 것으로 확인되었다. 따라서, 프라이머 FibL1 와 FibL2에 의한 피브로인 L-chain 유전자 다형성 염기서열 정보를 활용한다면 제한된 영역에서 누에 계통 구분을 위한 지표로 활용할 수 있을 것으로 사료된다. We have previously characterized the complete fibroin light chain gene from one of the silkworm race Baegokjam (Bombyx mori) and found two variable regions (FibL1, intron 2 ~ 3; FibL2, intron 6) with the primer sets designed to cover these variable regions. In this study, we tested the utility of these variable regions as genetic markers for classifying silkworm races. For the purpose, Europian races (Q, PK), Chinese races (C26, C31), Japanese races (N15, N9), and tropical races (SA2, SA5) were used in this experiment. The polymorphism of the FibL1 and FibL2 were divided into two and three types, respectively. The combination of the FibL1 and FibL2 polymorphisms were constant within the same races. The result suggest that the primer sets designed from two variable regions of fibroin light chain gene may be useful as the genetic markers for silkworm races.

정(井)자형 더블보-기둥 접합부의 전단강도에 대한 4변 구속의 영향

김량운(Kim. Lyang Woon),정창용(Chung, Chang-Yong),이수권(Lee, Soo Kueon),김상식(Kim, Sang Sik),최광호(Choi, Kwang Ho),이정윤(Lee, Jung Yoon) 한국콘크리트학회 2008 한국콘크리트학회 학술대회 논문집 Vol.2008 No.5

프리캐스트 슬래브를 이용한 평면확장공법에 대한 연구

김진평(Kim, jin-Pyeng),김상식(Kim, Sang-Sik),최광호(Choi, kwang-Ho),전병갑(Jeon, Byung-Gab),이정윤(Lee Jung-Yoon) 한국콘크리트학회 2006 한국콘크리트학회 학술대회 논문집 Vol.2006 No.5