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Suppression Subtractive Hybridization을 통한 Venturia Nashicola 접종후 ‘93-3-98’에서 유도된 저항성 후보 유전자 선발
천재안(Jae-An Chun),남가영(Ga-Young Nam),김세희(Se-Hee Kim),조강희(Kang-Hee Cho),김대현(Dae-Hyun Kim),최인명(In-Myeong Choi),신일섭(Il-Sheob Shin) 한국육종학회 2015 한국육종학회지 Vol.47 No.1
Suppression subtractive hybridization was carried out to identify resistant genes against to pear scab caused by Venturia nashicola using leaves harvested at 24 and 48 hours after inoculation into ’93-3-98’ (highly resistant) and ‘Sweat Skin’ (highly susceptible). As a result of the ESTs analysis, nine and 14 unique genes were expressed on 24H (tester, mRNA at 24hr after inoculation of ’93-3-98’; driver, one of ‘Sweet Skin’) and 48H (tester, mRNA at 48hr after inoculation of ’93-3-98’; driver, one of ‘Sweet Skin’), respectively and genes related to defense or stress response were accounted for 40% (24H) and 42% (48H). Differential expressed genes classifying into PR protein family were pathogenesis-related protein 1a, major allergen Pyr c1 and allergen mal d 1 at 24H and major allergen Mal d 1.03B at 48H, respectively. Major allergen Pyr c1, F-Box/kelch-repeat protein, Flavoprotein wrbA, and hypothetical protein POPTRDRAFT_783792 are expected to closely connecting to scab resistance of pear following strongly expressed in highly resistant cv. ‘Bartlett’ and ‘93-3-98’ compared with moderately susceptible cv. ‘Gamcheonbae’, susceptible cv. ‘Wonhwang’, and highly susceptible cv. ‘Niitaka’ and ‘Sweat skin’.
소모 전력이 적은 12비트 1MSps 축차 비교형 아날로그-디지털 변환기 설계
천재일(Jae-Il Chun),최예지(Ye-Ji Choi),류지열(Jee-Youl Ryu) 제어로봇시스템학회 2021 제어·로봇·시스템학회 논문지 Vol.27 No.1
In this paper, a low-power 12-bit 1MSps Successive-Approximation-Register (SAR) Analog-to-Digital Converter (ADC) is proposed to increase the integration degree. The proposed circuit is designed using the 1Poly-6Metal 0.13 ㎛ CMOS process, and operates at a supply voltage of 1.2 V. A pre-amplifier is incorporated to amplify both comparator and buffer, and offer flexible signal processing and good performance. The signal processing stage is compared to conventional circuits, so that fine signals can be captured for optimization. The proposed ADC showed very low power consumption (0.654 ㎽), good Figure of Merit (FOM) (0.98 fJ/conversion) and smaller die area (0.106 ㎟) compared with conventional results. This circuit also showed excellent Effective Number of Bits (ENOB) and high Signal-to-Noise Distortion Ratio (SNDR), 72.19 ㏈ and 11.69 bits respectively.
Design of Programmable Finite Impulse Response Filter
Jae-Il Chun(천재일),Ye-Ji Choi(최예지),Keun-Pil Kil(길근필),Myeong-U Sung(성명우),Shin-Gon Kim(김신곤),Murod Kurbanov(무로드 쿠르바노프),Delwar Tahesin Samira(델워 타헤신 사미라),Abrar Siddique(시디크 아브라르),Prangyadarsini Behera(파랑야다시 한국정보통신학회 2019 한국정보통신학회 종합학술대회 논문집 Vol.23 No.2
직류 오프셋 제거 회로를 가진 저 전력 프로그램 가능한 이득 증폭기 설계
최예지(Ye-Ji Choi),천재일(Jae-Il Chun),류지열(Jee-Youl Ryu) 제어로봇시스템학회 2020 제어·로봇·시스템학회 논문지 Vol.26 No.10
In this paper, a low-power gain control amplifier with a direct current offset cancellation circuit is proposed in order to amplify analog signals. The proposed PGA (Programmable Gain Amplifier) is less sensitive to external influences due to the inclusion of active load on differential amplifiers. The PGA connects a direct current offset cancellation circuit, based on the Miller effect, to a differential amplifier stage to reduce area and compensate for distortion or lack of linearity. This circuit is also designed to be adjustable in eight steps, from 4 dB to 60 dB, using gain controls. The proposed PGA is designed using the TSMC 0.13 μm CMOS process, and was verified by simulation in ADS (Advanced Design System) tool. Compared to conventional research results, the proposed PGA achieved a gain error of less than 0.1 dB and a lower consumption of 0.16 mW.
조강희(Kang Hee Cho),조광식(Kwang-Sik Cho),한점화(Jeom Hwa Han),김현란(Hyun Ran Kim),신일섭(Il Sheob Shin),김세희(Se Hee Kim),천재안(Jae An Chun),황해성(Hae-Sung Hwang) 한국원예학회 2013 원예과학기술지 Vol.31 No.6
중요 작물의 신속・정확하고 비용 면에서 효율적인 품종 판별은 실용적인 육종과 육종가의 권리 보호를 위해 필수적이다. 감 품종을 구분하는 전통적인 방법은 형태적인 특성 평가를 근거로 하지만 유전적으로 밀접하게 연관되어 있는 품종들은 형태적 형질에 의해 품종을 구별하기는 어렵다. 본 연구는 국내와 일본 감 32 품종을 판별할 수 있는 신뢰성 있는 DNA 마커를 개발하고자 수행하였다. 40종의 임의 프라이머를 이용한 RAPD 분석을 통해 품종 간 다형성을 나타내는 밴드 309종을 획득하였다. 프라이머에 따라 얻은 다형성 밴드 수는 4(OPP-08)-14(UBC159)개로 평균 7.7개였다. SCAR 마커로 전환하기 위해 57종의 RAPD 단편들을 선발하여 염기서열을 분석하였고 그 중 15종이 SCAR 마커로 전환되었다. 개발된 15종의 SCAR마커는 프라이머 조합에 따라 RAPD 단편과 동일한 크기나 작은 크기의 단일 밴드가 증폭되었다. 이들 마커 중 8종(PS225_200, PSN05_420, PSF13_523, PSN11_540, PS372_567, PS485_569, PSP08_635, PS631_735)의 조합을 적용하여 증폭산물의 수와 크기에 따라 감 32품종의 판별이 가능하였다. 새로 개발된 마커들은 감 품종 판별을 위해 신뢰성 있는 수단으로서 효과적으로 이용될 수 있을 것으로 판단된다. Precise, fast, and cost-effective identification of crop cultivars is essential for plant breeder’s rights. Traditional methods for identification of persimmon cultivars are based on the evaluation of sets of morphological characteristics. However, it is difficult to distinguish closely related cultivars using only morphological traits. This study was conducted to develop DNA markers for identification of the 32 persimmon cultivars in Korea and Japan. A total of 309 randomly amplified polymorphic DNA (RAPD) markers were identified using 40 different random primers. Various number of polymorphic bands ranged from 4 (OPP-08) to 14 (UBC159) were detected with an average of 7.7. Resulting 57 RAPD fragments were selected, and their sequences were determined for developing sequence characterized amplified region (SCAR) markers. As a result, 15 of 57 RAPD fragments were successfully converted to SCAR markers. Single polymorphic bands of the same size as or smaller than the RAPD fragments were amplified depending on SCAR markers. Among these markers, a combination of eight SCAR markers (PS225_200, PSN05_420, PSF13_523, PSN11_540, PS372_567, PS485_569, PSP08_635, and PS631_735) provided sufficient polymorphisms to identify 32 persimmon cultivars. These newly developed markers will be a fast and reliable tool to identify persimmon cultivars.