부산일부 보건소내원 당뇨환자의 지식 및 관리실태

천광훈 한국보건교육건강증진학회 2007 한국보건교육·건강증진학회 학술대회 발표논문집 Vol.- No.-

남해주민들의 운동행동변화단계에 따른 변화과정, 의사결정균형 및 자기효능감

천광훈,고광욱,김윤지,신용현 고신대학교 의과대학 2010 고신대학교 의과대학 학술지 Vol.25 No.2

Background: The purpose of this study was to examine the differences of the process of change, decisional balance. self-efficacy and perception of subjective health according to the stages of exercise behaviors suggested by transtheoretical medals among Namhae resident. For systemic continuos health promotion initiatives of public health center, basic information about exercise behaviors in need. Method: The stage of change, decision balance, self efficacy and other health-related information was surveyed from systematic sample of Namhae population. Result: The result of process of change was precontemplation (32.4%), contemplation (18.8%), preparation (18.8%), action (11.0%), and Maintenance (23.6%). Conclusion: There are corroborative evidence about the need for interpersonal organizational intervention to exercise behaviors of Namhae population and social alternative for the exercise behavior.

부산일부 보건소내원 당뇨환자의 지식 및 관리실태

천광훈(Cheon Gwang Hoon) 한국보건교육건강증진학회 2007 한국보건교육·건강증진학회 학술대회 발표논문집 Vol.- No.-

중,노년 한국인의 당화혈색소와 신체활동의 관련성

천광훈 ( Kwang Hoon Cheon ),고광욱 ( Kwang Wook Koh ) 대한보건협회 2011 대한보건연구 Vol.37 No.1

Objectives: This study set out to examine association between physical activity and HbAlc among Korean older adults and provide basic data to promote their physical activities for health. Methods: Of the second(2008) year data from the Fourth Korea National Health and Nutrition Examination Survey, the cases missing fasting blood glucose were excluded. Total 3,295 cases who were aged 45 or older were included to the study. Results: The diabetes group engaged in more moderate physical activities than vigorous ones. The borderline diabetes group and the no diabetes group had more total physical activities than the diabetes group. The female diabetes patients did more walking than their male counterparts. According to the multiple regression analysis results, the categories of physical activity had similar effects in predicting HbAlc in Korean Older Adults. Conclusions: More physical activities of various categories is need for diabetics Korean Older Adults. Especially moderate activity is recommended.

부산일부 보건소내원 당뇨환자의 지식 및 관리실태

천광훈(Cheon Gwang Hoon) 한국보건교육건강증진학회 2007 한국보건교육·건강증진학회 학술대회 발표논문집 Vol.- No.-

Cordyceps militaris Mushroom and Cordycepin Inhibit RANKL-Induced Osteoclast Differentiation

김진희,이혜진,강기성,천광훈,황귀서 한국식품영양과학회 2015 Journal of medicinal food Vol.18 No.4

Cordyceps militaris is a medicinal mushroom and its bioactive compound, cordycepin, is reported to have many pharmacological activities. The goal of this study was to investigate the effects of C. militaris extract (CME) and cordycepin on osteoclast differentiation in vitro and on an inflammatory bone loss in vivo. In RAW 264.7 cells, CME and cordycepin showed dose-dependent inhibition of receptor activator of the nuclear factor kappa B (NF-κB) ligand (RANKL)-induced osteoclast differentiation by TRAP (tartrate-resistant acid phosphatase) staining. Moreover, the mRNA expression of osteoclastogenesis-related genes (TRAP, cathepsin K, MMP-9, and NFATc1) was also inhibited by CME and cordycepin. Also, cordycepin significantly inhibited RANKL-induced phosphorylation of p38 and NF-κB, but not that of other members of mitogen-activated protein kinase families. To examine the effect of CME on bone loss in vivo, we used a mouse model of lipopolysaccharide (LPS)-mediated bone loss. Micro-CT analysis of the femurs showed that LPS treatment caused bone loss. However, bone loss was significantly attenuated in mice treated with CME. These results suggest that cordycepin or/and CME have inhibitory effects on osteoclast differentiation in vitro and that they suppress inflammatory bone loss in vivo.

Protective effect of Korean Red Ginseng against glucocorticoid-induced osteoporosis in vitro and in vivo

Jinhee Kim,Hyejin Lee,강기성,천광훈,황귀서 고려인삼학회 2015 Journal of Ginseng Research Vol.39 No.1

Background: Glucocorticoids (GCs) are commonly used in many chemotherapeutic protocols and play animportant role in the normal regulation of bone remodeling. However, the prolonged use of GCs resultsin osteoporosis, which is partially due to apoptosis of osteoblasts and osteocytes. In this study, effects ofKorean Red Ginseng (KRG) on GC-treated murine osteoblastic MC3T3-E1 cells and a GC-induced osteoporosismouse model were investigated. Methods: MC3T3-E1 cells were exposed to dexamethasone (Dex) with or without KRG and cell viabilitywas measured by the 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay. Realtimepolymerase chain reaction was performed to evaluate the apoptotic gene expression; osteogenicgene expression and alkaline phosphatase (ALP) activity were also measured. Western blotting wasperformed to evaluate the mitogen-activated protein kinase (MAPK) proteins. A GC-induced osteoporosisanimal model was used for in vivo study. Results and conclusion: The MTT assay revealed that Korean Red Ginseng (KRG) prevents loss of cellviability caused by Dex-induced apoptosis in MC3T3E1 cells. Real-time polymerase chain reaction datashowed that groups treated with both Dex and KRG exhibited lower mRNA levels of caspase-3 and -9,whereas the mRNA levels of Bcl2, IAPs, and XIAP increased. Moreover, groups treated with both Dex andKRG demonstrated increased mRNA levels of ALP, RUNX2, and bone morphogenic proteins as well asincreased ALP activity in MC3T3-E1 cells, compared to cells treated with Dex only. In addition, KRGincreased protein kinase B (AKT) phosphorylation and decreased c-Jun N-terminal kinase (JNK) phosphorylation. Moreover, microcomputed tomography analysis of the femurs showed that GC implantationcaused trabecular bone loss. However, a significant reduction of bone loss was observed in the KRGtreatedgroup. These results suggest that the molecular mechanism of KRG in the GC-induced apoptosismay lead to the development of therapeutic strategies to prevent and/or delay osteoporosis.

Protective Effect of Processed Panax ginseng, Sun Ginseng on UVBirradiated Human Skin Keratinocyte and Human Dermal Fibroblast

이혜진,이주엽,송규춘,김진희,박정일,천광훈,황귀서 고려인삼학회 2012 Journal of Ginseng Research Vol.36 No.1

In this study, we investigated the protective effects of processed Panax ginseng, sun ginseng (SG) against the UVB-irradiation on epidermal keratinocytes and dermal fi broblasts. Pretreatment of SG in HaCaT keratinocytes and human dermal fi broblasts reduced UVB-induced cell damage as seen by reduced lactate dehydrogenase release. We also found that SG restored the UVB-induced decrease in anti-apoptotic gene expression (bcl-2 and bcl-xL) in these cells, indicating that SG has an anti-apoptotic effect and thus can protect cells from cell death caused by strong UVB radiation. In addition, SG inhibited the excessive expression of c-jun and c-fos gene by the UVB in HeCaT cells and human dermal fi broblasts. We also demonstrated that SG may exert an anti-infl ammatory activity by reducing the nitric oxide production and inducible nitric oxide synthase mRNA synthesis in HaCaT keratinocytes and human dermal fi broblasts. This was further supported by its inhibitory effects on the elevated cyclooxygenase-2 and tumor necrosis factor-α transcription which was induced by UVB-irradiation in HaCaT cells. In addition, SG may have anti-aging property in terms of induction of procollagen gene expression and inhibition of the matrix metalloprotease-1 gene expression caused by UVBexposure. These fi ndings suggest that SG can be a potential agent that may protect against the dermal cell damage caused by UVB. In this study, we investigated the protective effects of processed Panax ginseng, sun ginseng (SG) against the UVB-irradiation on epidermal keratinocytes and dermal fi broblasts. Pretreatment of SG in HaCaT keratinocytes and human dermal fi broblasts reduced UVB-induced cell damage as seen by reduced lactate dehydrogenase release. We also found that SG restored the UVB-induced decrease in anti-apoptotic gene expression (bcl-2 and bcl-xL) in these cells, indicating that SG has an anti-apoptotic effect and thus can protect cells from cell death caused by strong UVB radiation. In addition, SG inhibited the excessive expression of c-jun and c-fos gene by the UVB in HeCaT cells and human dermal fi broblasts. We also demonstrated that SG may exert an anti-infl ammatory activity by reducing the nitric oxide production and inducible nitric oxide synthase mRNA synthesis in HaCaT keratinocytes and human dermal fi broblasts. This was further supported by its inhibitory effects on the elevated cyclooxygenase-2 and tumor necrosis factor-α transcription which was induced by UVB-irradiation in HaCaT cells. In addition, SG may have anti-aging property in terms of induction of procollagen gene expression and inhibition of the matrix metalloprotease-1 gene expression caused by UVBexposure. These fi ndings suggest that SG can be a potential agent that may protect against the dermal cell damage caused by UVB.

Designing Tyrosinase siRNAs by Multiple Prediction Algorithms and Evaluation of Their Anti-Melanogenic Effects

권옥선,권수정,김진상,이건봉,맹한주,이정미,황귀서,차혁진,천광훈 한국응용약물학회 2018 Biomolecules & Therapeutics(구 응용약물학회지) Vol.26 No.3

Melanin is a pigment produced from tyrosine in melanocytes. Although melanin has a protective role against UVB radiationinduced damage, it is also associated with the development of melanoma and darker skin tone. Tyrosinase is a key enzyme in melanin synthesis, which regulates the rate-limiting step during conversion of tyrosine into DOPA and dopaquinone. To develop effective RNA interference therapeutics, we designed a melanin siRNA pool by applying multiple prediction programs to reduce human tyrosinase levels. First, 272 siRNAs passed the target accessibility evaluation using the RNAxs program. Then we selected 34 siRNA sequences with ΔG ≥-34.6 kcal/mol, i-Score value ≥65, and siRNA scales score ≤30. siRNAs were designed as 19-bp RNA duplexes with an asymmetric 3’ overhang at the 3’ end of the antisense strand. We tested if these siRNAs effectively reduced tyrosinase gene expression using qRT-PCR and found that 17 siRNA sequences were more effective than commercially available siRNA. Three siRNAs further tested showed an effective visual color change in MNT-1 human cells without cytotoxic effects, indicating these sequences are anti-melanogenic. Our study revealed that human tyrosinase siRNAs could be efficiently designed using multiple prediction algorithms.