내염성 담배 캘러스의 광합성능과 peroxidase의 활성

차현철 ( Hyeon Cheol Cha ) 단국대학교 신소재기술연구소 2004 신소재 연구논집 Vol.12 No.2

내염성 담배 캘러스에 대한 NaCl 의 효과

차현철(Hyeon Cheol Cha),손종현(Jong Hyeon Sohn),김갑식(Kap Sik Kim),최광태(Kwang Tae Choi),이광웅(Kwang Woong Lee) 한국식물학회 1993 Journal of Plant Biology Vol.36 No.1

Effects of various NaCl concentrations on salt-tolerant callus of tobacco were investigated. Selection of NaCl-tolerant (S) callus was conducted by subculturing Nicotiana tabacum cv. BY 4 callus in 200 mM NaCl-containing MS medium for more than 18 months. In spite of the long subculture period, characteristics of salt tolerance were maintained very stably. Significant differences were found in ion contents of each callus which was subcultured with treatment of various NaCl concentrations: Na^+ and Cl^- became higher but Mg^2+, Ca^2+ and K^+ becmae lower with the increasing external salt contents. Therefore, the ratios of Na^+/Ca^2+ and Na^+/K^+ also increased resulting close to those of halophytic property. The contents of chlorophylls and carotenoids in S callus were estimated to 3.1 and 2.9 times more, respectively, that those of non-selected (NS) callus (control). The higher content of external NaCl tended to increase the amount of water soluble proteins and to decrease the amounts of the total sugars, reducing sugars and free amino acids. The activity of peroxidase was increased with higher contents of external NaCl in S callus, but it was maintained at a higher level than S callus at lower NaCl, followed by a subsequent decrease above 80 mM NaCl in NS callus. These results suggest that S callus may have a biological system converting energy source to efficient growth leading to reduction of the growth inhibition under stress environment.

콩 원형질체내로의 (原形質體內) 담배 엽록체 이입

차현철(Hyeon Cheol Cha),조성호(Sung Ho Cho),이광웅(Kwang Woong Lee) 한국식물학회 1982 Journal of Plant Biology Vol.25 No.4

Chloroplasts isolated from tobacco (Nicotiana tabacum L. cv. Virginia 115) leaves have been transferred into protoplasts of soybean (Glycine max Merr. cv. Jangyeop) suspension-cultured cells with the help of polyethlene glycol (PEG). The increased yield in protoplasts of chloroplast uptake was depended upon the concentration of both PEG 4,000 and PEG 6,000. The highest yield (36%) occurred at 50% of both PEG, and the yield was decreased above this concentration. The rate of uptake with the incubation time was highest at one hour, then decreased. The process of the chloroplast uptake into the protoplasts was similar with that of a protoplast fusion, except forming invagination during uptake.

며느리배꼽 잎 유래 캘러스의 부정근 형성에 미치는 지베렐린의 작용

김현(Hyeon Kim),차현철(Hyeon-Cheol Cha) 한국생명과학회 2015 생명과학회지 Vol.25 No.4

본 연구는 항산화 기능이 뛰어나다고 알려진 며느리배꼽(Persicaria perfoliata)의 캘러스를 사용하여, 어떤 식물호르몬이 부정근 형성에 영향을 미치는지 알아보기 위한 실험이다. 며느리배꼽의 잎으로부터 캘러스를 유도하고 이로부터 부정근 형성을 관찰하였다. 캘러스 유도의 최적 조건을 구한 결과 1% sucrose, 4.5 μM 2.4-D, 1/2 MS였다. 어떤 식물호르몬이 이 식물의 캘러스에서 부정근 형성 효과를 가지고 있는지 알아보기 위하여 GA3, IAA, 2iP, 2,4-D를 캘러스 조직에 각각 첨가한 결과, GA3와 IAA를 첨가한 배지에서만 부정근이 나타났음을 확인 할 수 있었다. 이를 자세히 조사하기 위해서 GA3와 IAA의 농도별(0.1, 1, 10 mg/l) 부정근 형성, 길이 및 직경을 알아본 결과, 높은 수준의 GA3 또는 낮은 수준의 IAA처리에서 더 많은 부정근 형성을 보였다. 이 두 호르몬 중 어떤 것이 더욱 더 중요한 역할을 하는지 알아보기 위하여 옥신 유입 저해제인 NPA와 지베렐린 생합성 저해제인 PBZ를 처리한 실험을 한 결과, GA3가 IAA 보다 부정근 형성에 더욱 더 중요한 역할이라는 것을 알았다. 하여 지베렐린의 수준을 증가 또는 감소시킨다고 알려진 식물호르몬(IAA, 2iP, kinetin, ABA)들을 GA3와 혼용 처리한 결과, 이 역시 지베렐린이 부정근을 형성하는데 중요한 역할을 한다는 것을 재확인 하였다. 본 연구는 지베렐린이 며느리배꼽의 캘러스에서 부정근 형성을 증진시킨다는 것을 처음으로 밝힌다. This study was carried out to investigate the action of phytohormones which influence the adventitious root formation of calli originating from the leaves of Persicaria perfoliata. The optimal medium condition for callus formation was ½-strength MS, 1% sucrose, and 4.5 μM 2,4-D. In order to determine which phytohormones had an effect on the adventitious root formation, the calluses were cultured in various media with different kinds of phytohormones. As a result, the medium with GA3 or IAA was shown to induce root formation. To deeply investigate the effects of GA3 and IAA, calli were cultured in 0.1, 1, and 10 mg/l levels of phytohormones. Numbers of roots formed per callus were 10.9, 14.2, 22.6 in GA3, 5.8, 3.9, 1.1 in IAA, respectively. Therefore, the higher GA3 or the lower IAA concentration, the more roots formed. To confirm this role of GA3 we tested with inhibitors PBZ and NPA. GA3 with PBZ resulted in reduction by 52.4~69.4% compared to GA3 alone. In contrast, GA3 with NPA resulted in an increase by -8~45.6% compared to GA3 alone in root formation. Also, results were determined on the effect of GA3 with other phytohormones on root formation. Kinetin, 2iP and ABA with GA3 had a negative effect, but IAA with GA3 showed a similar result to GA3 alone. From these results we infer GA plays a key role and auxin has subsidiary activity on adventitious root formation. This is the first report that indicates GA3 promotes adventitious root formation from calli in P. perfoliata.

세포융합시 전계하에서 식물세포가 받는 영향에 관한연구

이상훈,이연민,차현철,Lee, Sang-Hoon,Lee, Yon-Min,Cha, Hyeon-Cheol 대한의용생체공학회 1996 의공학회지 Vol.17 No.2

The objective of this paper is to investigate the effect of AC field on the protoplast of plant cells. The results of investigation will be the basis for the development of etectric cell fusion device. For the experiment, we made the electrode and AC and DC pulse generator and observed the behavior of the protoplasts through the inverted microscope which is connected to the monitor and video recorder by the CCD camera. As a result, the numbers of rotating, moving and destructed protoplasts and viability of the protoplasts have close relation to the amplitude of AC field, while the rotation rate is closely related to the frequency of AC pulse.

외래식물에 의한 대학 교정 생태계의 교란

김세은,이효혜미,차현철,Kim, Seeun,Lee, Hyohyemi,Cha, Hyeon-Cheol 응용생태공학회 2015 Ecology and resilient infrastructure Vol.2 No.1

귀화식물은 외국으로부터 들어와 야생에서 자라는 외래식물로서 대부분 번식력이 뛰어나 우리 생태계에서 서식지를 확대하며 토종식물들을 위협하여 문제가 되고 있다. 본 연구는 유동인구가 많고, 비교적 큰 규모의 생물서식공간을 제공하는 대학교 캠퍼스에 자연 생태계로 유입되어 귀화된 외래종이 얼마나 많이 서식하고, 어떠한 물리적 환경 요인이 귀화식물의 분포에 가장 많은 영향을 미치는지를 밝히기 위하여 연구되었다. 캠퍼스는 천안시 소재의 단국대학교, 백석대학교, 상명대학교, 호서대학교를 대상으로 각 교정에 출현하는 귀화된 외래종 (원예종 제외) 및 고유종을 조사하고, 교지와 녹지 면적, 각 학교 사이의 거리 및 고속도로와 거리를 조사하였다. 발견된 총 식물종과 외래종 수는 각각 189종과 43종이었고, 단국대 136종과 35종, 호서대 108종과 25종, 상명대 103종과 31종, 백석대 97종과 26종이 출현하였다. 각 대학 교정에서 외래종의 풍부도는 고속도로와 가까울수록 높았다. 또한 대학 간의 도보거리가 가까울수록 출현 외래종 식물상의 유사도가 높아지는 경향을 보였다. 이상의 결과로 보아 대학 교정에서 외래종에 의한 생태계 교란이 심화되고 있으며, 특히 인간 활동이 외래종의 분포와 밀접한 연관성을 가짐을 알 수 있었다. Some of alien plants, which were introduced from foreign countries, have caused problems in Korea. Invasion of these alien plants in the ecosystem threatens the habitat of endemic species, reducing biodiversity, and causing a disturbance in the ecological system. In urbanized areas of campus universities, a diverse range of organisms were found and a comparison between the sites, near roads or housing sites were made because the campuses provided a large biotope. Although the campus had been exposed to interferences like gardening, it was also a place for most organisms to live in an active floating population due to free access. This research investigated the flora of alien plants that appeared in Beakseok University, Sangmyung University, Hoseo University and Dankook University, and relationship between the distribution of alien plants and the campus and green areas and distance from the highway. The total number of plant species and naturalized species found in the four universities was 189 and 43 species. Those of Dankook University were 136 and 35 species, Hoseo 108 and 25 species, Sangmyung 103 and 31 species, and Baekseok 97 and 26 species, respectively. The abundance of natural plants for each respective university tended to be higher as it became closer to the highway. Also, the closer the walking distance to the university, the greater the tendency for the degree of similarity to be higher. As a result, we may conclude that the distribution of alien plants and anthropogenic activities may be closely related.

흑한우와 한우 및 수입우를 판별하기 위한 multiplex PCR 기술

김찬수(Chan-Su Kim),고정문(Jung-Moon Ko),차현철(Hyeon-Cheol Cha),박중국(Joong Kook Park),정준(Joon Jeong) 한국생명과학회 2014 생명과학회지 Vol.24 No.8

본 연구는 현행 한우확인시험법에 이용되는 Microsatellite (MS)와 소의 유전형질 중 품종 판별에 주로 이용되는 Melanocortin Receptor 1 (MC1R) 유전자상의 Single Nucleotide Polymorphism (SNP)를 분석하여 설계한 Primer를 Multiplex PCR을 활용하여 소의 품종을 판별하였다. MC1R 유전자형은 E<SUP>D</SUP>, E<SUP>+</SUP>, e형의 3Type으로 나뉘며 E<SUP>D</SUP>/E<SUP>D</SUP>, E<SUP>D</SUP>/E<SUP>+</SUP>, E<SUP>D</SUP>/e, E<SUP>+</SUP>/E<SUP>+</SUP>, E<SUP>+</SUP>/e, e/e의 6가지 유전자형을 가진다. E<SUP>D</SUP>유전자형은 외래종이 지닌 유전자형으로 E<SUP>D</SUP>/E<SUP>D</SUP>, E<SUP>D</SUP>/E<SUP>+</SUP>, E<SUP>D</SUP>/e이 이에 속하며, e유전자형은 한우가 지닌 유전자형으로 e/e의 유전자형을 가진다. 흑한우의 경우 E<SUP>D</SUP>, E<SUP>+</SUP>, e의 모든 유전자형을 가지고 있으나 이는 교잡에 의해 나타난 것으로 보이며 E<SUP>+</SUP>유전자형이 흑한우 고유의 유전자형으로 추정되어 본 연구에서는 이에 따라 E<SUP>+</SUP>/E<SUP>+</SUP>, E<SUP>+</SUP>/e의 유전자형을 흑한우로 분류하였다. 그러나 E<SUP>D</SUP>, E<SUP>+</SUP>의 경우 단순 PCR기법만으로는 그 판별에 어려움이 있어, MS Maker를 활용한 다형성 분석을 통해 흑한우와 수입우를 판별할 수 있는 새로운 Primer를 설계하였으며, 이를 통해 소의 품종을 판별하였다. For the identification of the Jeju black cattle, Hanwoo and imported beef, we performed a multiplex polymerase chain reaction (PCR) associated with microsatellite (MS) and melanocortin 1 receptor (MC1R) gene. The MC1R gene plays an important role in regulation of the melanin synthesis within mammalian melanocytes. MC1R encoded by extension (E) locus was almost fixed with recessive red e allele in the Hanwoo. We estimated that the specific genotypes (E<SUP>+</SUP>/E<SUP>+</SUP>, E<SUP>+</SUP>/e) of MC1R gene were characteristic genotypes of Jeju black cattle. But the PCR products resulted from using the MC1R gene derived primers only are not sufficient to identify Jeju black cattle from other relatives. We performed two times of successive multiplex PCR to provide a more accurate result for the identification of Jeju black cattle. The results suggest that two types of successive multiplex PCR methods using MC1R gene and Microsatellite derived primer set will be more useful to identification of Jeju black cattle, Hanwoo and imported beef.

Mass Production of Herbal Plant, Peucedanum japonicum, Via Somatic Embryogenesis

Jung-moon Ko(고정문),Hye-Jeong Park(박혜정),Byeong-Mee Min(민병미),Hyeon-Cheol Cha(차현철) 한국원예학회 2005 한국원예학회 학술발표요지 Vol.2005 No.5

완두와 보리의 엽육세포 원형질체 분리 및 융합

이광웅(Kwang Woong Lee),조성호(Sung Ho Cho),차현철(Hyeon Cheol Cha) 한국식물학회 1980 Journal of Plant Biology Vol.23 No.2

The optimal conditions for the protoplast isolation from the leaves of pea (Pisum sativum L. cv. Sparkle) and barley (Hordeum vulgare L. cv. Baecdong) were determined in order to achieve a somatic hybridization between teo species. It was revealed that the use of 0.5M sorbitol as an osmoticum was appropriate for pea. The yield of intact protoplasts was the highest (40%) when pea leaves were incubated in the enzyme solution for 4 hours. In case of barley, the optimal concentrations of cellulase, pectinase and mannitol as the enzyme solution were 2%, 1% and 0.35M, respectively. And the yield of barley protoplasts was the highest (87%) when leaves were incubated in this enzyme solution for 3.5 hours. A fusion of protoplasts from pea and barley was induced by PEG treatment enriched with calcium salts within 60 minutes.

Flavonol Compositions of Grapevines by Cultivars and Environmental Factors

Hye-Jeong Park(박혜정),Jin-Hee Lee(이진희),Jung-Moon Ko(고정문),Tae-Hyun Kim(김태현),Hyeon-Cheol Cha(차현철) 한국원예학회 2005 한국원예학회 학술발표요지 Vol.2005 No.5