Comparison of maturation and secondary spawning of fleshy shrimp Penaeus chinensis fed different kinds of diets

진영국,전유현,Sukyoung Kim,정은하,Su-Kyoung Kim 한국수산과학회 2022 Fisheries and Aquatic Sciences Vol.25 No.12

The study carried out a 15-day feeding trial of the fleshy shrimp broodstock that have just finished their first spawn using five different maturation feed types (three types of manufactured feed, fresh feed, and commercial feed) to induce multiple spawning in the shrimp and then analyzed and compared hemolymph levels of total protein, total lipid, hemocyanin, glucose, and cholesterol as well as ovary histology between the treatments to identify the potential of the experimental diets to be utilized as maturation feed. The study found that the manufactured diets of polychaetes + clam and polychaetes + clam + squid resulted in significantly higher levels of lipid and protein in shrimp hemolymph suggesting an indicator of multiple spawning in the fleshy shrimp. The other manufactured diet of polychaets + squid led to the highest 2nd spawning with significantly lower levels of lipid, protein, and cholesterol in shrimp hemolymph. The fresh feed treatment showed similar hematological as well as histological responses to the manufactured diet of polychaetes + squid, but with a significantly lower spawning volume compared to the other treatments. The study demonstrated that the manufactured diets could replace fresh feed as maturation feed and attain a higher shrimp maturation than the commercial feed.

이사천에서 채집된 피라미, Zacco platypus의 생식과 기관계 구조 이상

진영국,신순호,이제봉,이재우,안철민,이정식 환경독성보건학회 2006 환경독성보건학회지 Vol.21 No.1

실내 수조에서 북방전복과 해삼의 복합사육에 따른 생존과 성장

진영국,오봉세,박민우,조재권,정춘구,김태익 한국패류학회 2011 The Korean Journal of Malacology Vol.30 No.4

The present study investigated survival and growth of the abalone, Haliotis discus hannai and sea cucumber, Stichopus japonicus co-cultured and supplied with abalone formula feed (AFF) and dry sea tangle (DST) in indoor tank. During the experimental period from April 20 to July 15 in 2011, we used abalone with shell length of 28.54 ± 1.23 mm and total weight of 2.92 ± 0.26 g. The sea cucumber used in the experiment weighed 3.0 ± 0.2 g in wet weight. For the experiment, 3 types of feeds including AFF, DST and AFF-DST mixed (1:1) group were supplied to abalone and sea cucumber. During the experiment, survival of abalone reached over 97.0% and 87.0% for sea cucmber in three experimental tanks. Survival of the abalone was found to be higher in DST group, while it was lower among sea cucumber, although the survival was not significantly different among three experimental group. Growth of abalone fed with AFF was significantly higher, while the growth rae of sea cucumber was highest in the tank fed with AFF+DST mixed diet (P < 0.05).

아연의 체내축적이 대복의 생존, 운동성, 성장 및 기관계 구조에 미치는 영향

진영국,주선미,이재우,유준,이정식 환경독성보건학회 2006 환경독성보건학회지 Vol.21 No.2

나프탈렌에 노출된 바지락, Ruditapes philippinarum의 생존 및 생식지표 변화

진영국,김태익,명정인,황형규,박민우 한국패류학회 2012 The Korean Journal of Malacology Vol.28 No.4

Manila clam, Ruditapes philippinarum (SL; 32.74 ± 2.18 mm, TW; 8.29 ± 1.41 g, N; 450) were exposed to various concentrations of naphthalene for 20 weeks. Exposure concentrations of naphthalene established control, solvent control (ethanol), 30, 60, 90 and 120 μg Nap L-1 following results of 96h acute exposure. After exposure during 20 weeks, survival rate of the manila clam was lowed in 60, 90 and 120 μg Nap L-1 exposure group compared control group. Also, sex ratio of male was higher in 60 μg Nap L-1 exposure group (χ2 = 5.492, P < 0.05) but lowed 90 μg Nap L-1 exposure group (χ2 = 4.214, P < 0.05) and 120 μg Nap L-1 exposure group (χ2 = 30.118, P < 0.05). Gonad development was delayed in female (> 60 μg Nap L-1) and male (> 30 μg Nap L-1). Intersex was 16.28% in female, 1.68% in male. In this result, naphthalene caused survival decrease, imbalance of sex ratio, delay of gonad development of the manila clam. Also, chronic exposure to naphthalene suggested abnormal effects in reproduction of the manila clam. 나프탈렌이 바지락 (각장 32.74 ± 2.18 mm, 전중 8.29 ±1.41 g) 의 생존, 성비 및 생식소발달에 미치는 영향에 대해연구하였다. 노출농도는 대조구, solvent 대조구 (에탄올), 30,60, 90, 120 μg Nap L-1 였으며, 노출기간은 20주였다. 노출 종료 후 바지락의 생존율은 60 μg Nap L-1 이상의 농도에서 감소하였다. 성비는 대조구와 비교해 solvent 대조구 (에탄올)와 30 μg Nap L-1 실험구는 별다른 차이를 보이지 않았지만, 60 μg Nap L-1 실험구의 경우 수컷의 비율이 높았다. 그 후 농도가 높아질수록 수컷의 비율이 감소하였다. 생식소 발달단계는 암컷의 경우 60 μg Nap L-1 이상의 농도에서,수컷의 경우 30 μg Nap L-1 이상의 농도에서 발달이 지연되는 결과를 보였다. 생식소에서 관찰된 intersex는 암컷에서16.28%, 수컷에서 1.68%를 나타냈다. 이러한 결과들을 볼 때나프탈렌은 바지락의 생존 뿐 만 아니라 성비와 생식에 영향을주는 것으로 보인다

실내 수조에서 북방전복과 해삼의 복합사육에 따른 생존과 성장

진영국,오봉세,박민우,조재권,정춘구,김태익,Jin, Young-Guk,Oh, Bong-Se,Park, Min-Woo,Cho, Jae-Kwon,Jung, Choon-Koo,Kim, Tae-Ik 한국패류학회 2011 The Korean Journal of Malacology Vol.27 No.4

2011년 4월 20일부터 7월 15일까지 전복배합사료와 건다시마를 먹이원으로 한 북방전복과 해삼의 실내 복합사육에서 생존율과 성장에 어떠한 영향을 받는지 관찰하였다. 실험에 사용된 북방전복의 크기는 각장 $28.54{\pm}1.23mm$, 전중량 $2.92{\pm}0.26g$ 이었고, 해삼은 습중량 $3.0{\pm}0.2g$ 이었다. 먹이는 전복배합사료 실험구, 건다시마 실험구 및 전복배합사료와 건다시마 혼합 실험구로 설정하였다. 북방전복과 해삼의 생존율은 모든 실험구에서 각각 97.0%, 87.0% 이상을 보였다. 북방전복의 생존율은 건다시마 실험구에서 가장 높은 반면 해삼은 가장 낮았지만 유의적인 차이는 보이지 않았다. 성장은 북방전복의 경우 전복배합사료 실험구에서, 해삼의 경우 전복배합사료와 건다시마를 동시에 공급한 실험구에서 가장 빨랐다. The present study investigated survival and growth of the abalone, Haliotis discus hannai and sea cucumber, Stichopus japonicus co-cultured and supplied with abalone formula feed (AFF) and dry sea tangle (DST) in indoor tank. During the experimental period from April 20 to July 15 in 2011, we used abalone with shell length of $28.54{\pm}1.23mm$ and total weight of $2.92{\pm}0.26g$. The sea cucumber used in the experiment weighed $3.0{\pm}0.2g$ in wet weight. For the experiment, 3 types of feeds including AFF, DST and AFF-DST mixed (1:1) group were supplied to abalone and sea cucumber. During the experiment, survival of abalone reached over 97.0% and 87.0% for sea cucumber in three experimental tanks. Survival of the abalone was found to be higher in DST group, while it was lower among sea cucumber, although the survival was not significantly different among three experimental group. Growth of abalone fed with AFF was significantly higher, while the growth rate of sea cucumber was highest in the tank fed with AFF+DST mixed diet (P < 0.05).

개조개, Saxidomus purpuratus의 중간육성 및 씨뿌림된 치패의 성장과 생존

진영국,오봉세,정춘구,김태익,박민우 한국패류학회 2011 The Korean Journal of Malacology Vol.27 No.3

We studied possibility of the sowing culture at the bottom after intermediate culture as a method for raising the survival of the Purplish Washington clam, Saxidomus purpuratus. The pearl net (35 cm (W) × 35 cm (B) × 20 cm (H)) for S. prupuratus spat during the period of intermediate culture (hanging culture) from April to October in 2010. The pearl net (100 inds./net) was installed at two stations (Namhae and Pohang) being about 3-4 m in water depth. After hanging culture, S. prupuratus spat sowed in the bottom of the Gangjin Bay. The survivals (%) of intermediate culture of this species at Namhae station and Pohang station werw 73% and 74%, respectively. Daily growth rate of mean shell length and mean weight in Namhae station werw higher than those in Pohang station. After sowing at the bottom of the Gangjin Bay, its surviva (%) showed 73.98% in January 2011. These results suggested the possibility of the intermediate culture as hanging culture for raising survival rate of S. prupuratus.

인위적 수온 상승에 노출된 조피볼락 (Sebastes schlegelii) 치어의 생존율 및 스트레스 반응

진영국,길현우,김대중,황형규,김효원 한국어류학회 2023 韓國魚類學會誌 Vol.35 No.4

본 연구는 조피볼락 치어를 대상으로 인위적인 수온 상승에 따른 생존율, 혈중 cortisol, glucose, GOT, GPT 농도 및 아가미와 간의 조직학적 변화를 조사하였다. 실험 수온은 26℃, 28℃, 30℃ 및 31℃로 설정하였고, 수온은 23℃ (initial)~28℃까지는 1일에 1℃씩, 28~31℃까지는 3일에 1℃씩 조절하였다. 실험은 각 실험 수온에서 7일 동안 수행하였고, 실험기간 동안 먹이는 공급하지 않았다. 그 결과, 26℃와 28℃는 폐사가 발생하지 않았으나, 30℃는 노출 4일째부터 폐사가 발생하여 실험종료시 1.5%의 생존율을 보였고, 31℃는 노출 1일째부터 폐사가 발생하여 노출 2일째 전량 폐사하였다. 혈중 스트레스 관련 지표를 분석한 결과, 혈중 cortisol과 glucose 농도는 수온이 높을수록 증가하였고, 31℃에서 initial보다 유의하게 감소하였다 (p<0.05). 혈중 GPT 농도는 모든 실험구 사이에 유의한 차이를 보이지 않았으나 (p<0.05), 혈중 GOT 농도는 31℃에서 유의하게 증가하였다 (p<0.05). 간과 아가미의 조직학적 관찰 결과, 간과 아가미는 모두 23℃ (initial) 및 26℃에서 정상적인 조직상을 보였으나, 28℃는 간세포의 비대화 및 아가미 새판 상피세포의 증식과 새판 상피의 탈락, 30℃는 간세포들이 응축 및 아가미 새판의 융합, 31℃는 간 조직 내 충혈 및 일부 간세포의 괴사 및 아가미 새엽과 새판의 괴사가 관찰되었다. This study aimed to investigate the survival rates, hematologic responses, and histological responses of juvenile Korean rockfish (Sebastes schlegelii) exposed to artificial increase of water temperature. The water temperature was incrementally raised from the initial 23°C to 26°C, 28°C, 30°C, and 31°C, with a 1°C increase every 24 hours. The fish were exposed to each water temperature setting for a period of seven days. No mortality was observed at 26°C and 28°C. However, at 30°C, mortality began on the 4th day of exposure, with an overall survival rate of 1.5% at the end of the seventh day. At 31°C, mortality occurred as early as the first day of exposure, and all fish had perished by the second day. Plasma cortisol and glucose concentrations increased as water temperature rose, with a significant decrease observed at 31°C. No significant difference in plasma GPT concentration was observed across the various experimental temperatures. In contrast, plasma GOT concentration significantly increased at 31°C. Histological examination revealed that both the liver and gills exhibited normal histology at the initial temperature of 23°C and at 26°C. However, at 28°C hepatocellular hypertrophy and gill lamellar epithelial hyperplasia and epithelial cell lifting were observed. At 30°C, hepatocellular condensation and gill lamellar fusion were noted. Finally, at 31°C, severe histological changes were observed, including hepatocellular necrosis, liver congestion, and gill filament necrosis.

WSF(Water Soluble Fraction)가 참전복, Haliotis discus hannai의 유생 및 치패발생에 미치는 영향

진영국,전제천,신윤경,오봉세,정춘구 한국패류학회 2010 The Korean Journal of Malacology Vol.26 No.2

This study was carried out to investigate the toxic effect of WSF (Water Soluble Fraction) on development time, development rate, attachment rate, survival rate and growth of the larvae during the early life stage of the abalone, Haliotis discus hannai. Also, observed effect of water temperature on the survival rate and histological change of gill in the early young shell. In the abalone, it takes around 12 hours in all experimental groups. Development and attachment rate of the abalone significantly lower more than 0.4 mg/L WSF compared to control group (P < 0.05). Survival rate of abalone larva and spot was significantly lower more than 0.4 mg/L and 2.4 mg/L WSF compared to control group, respectively (P < 0.05). Shell growth of the abalone were significantly lower more than 2.4 mg/L WSF compared control group (P < 0.05). Survival rate lower more than 25℃ exposure group compared water temperature 17℃ exposure group in the early young shell. The gill of abalone exposed water temperature 17℃ and 25℃ was showed atrophy of nucleus and breakdown of the filament, vacuolation of filament epithelial cell. 원유의 WSF가 참전복의 유생 및 치패 발생에 미치는 영향을 알아보기 위하여 발생소요시간, 발생률 및 부착률, 생존율,유생 및 부착치패의 각성장 그리고 수온에 따른 치패의 생존율및 아가미의 조직학적 변화를 조사하였다. 발생소요시간은 대조구에 비해 노출구에서 느리게 나타났다. 발생률 및 부착률은0.4 mg/L 이상의 농도에서 대조구에 비해 유의적인 감소를보였다 (P < 0.05). 참전복 유생의 생존율은 0.4 mg/L 이상에서, 부착치패는 2.4 mg/L 이상에서 유의적인 감소가 나타났다 (P < 0.05). 각성장은 2.4 mg/L 이상의 농도에서 대조구에 비해 감소하였다 (P < 0.05). 수온에 따른 치패의 생존율은 17℃와 25℃ 모두 노출농도에서 감소하였으며, 특히 고수온에서 전체적인 생존율이 낮게 나타났다. 또한 아가미에서나타난 조직학적 변화는 주로 새엽 상피세포 핵의 위축 및 상피세포의 공포화 그리고 상피층의 괴사를 동반한 붕괴 등이 관찰되었으며, 이는 17℃에 노출된 개체들 보다는 고수온인 25℃에 노출된 개체들에서 심하게 나타났다.

잿방어, Seriola dumerili 피부계의 전자현미경적 구조

진영국 ( Young Guk Jin ),이재우 ( Jae Woo Lee ),이정식 ( Jung Sick Lee ) 한국어류학회 2005 韓國魚類學會誌 Vol.17 No.4