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Short Communication : 도축검사 후 지방성 병변과 축산물 품질 연관성 조사
성창민 ( Chang Min Sung ),지태경 ( Tae Kyung Ji ),최종욱 ( Jong Wook Choi ),박다해 ( Da Hae Park ),박대희 ( Dae Hee Park ),김현중 ( Hyun Joong Kim ),김용환 ( Yong Hwan Kim ),김은선 ( Eun Sun Kim ) 한국가축위생학회 2013 韓國家畜衛生學會誌 Vol.36 No.2
The survey was conducted to investigate the relation between slaughter inspection results and the quality of cattle carcasses by inquiring animal products grading service results of Korea Institute for Animal Products Quality Evaluation (KAPE) about partial-disposed cattle carcasses after dismantling inspection. Of the 13,586 slaughtered cattle, 1,343 (9.9%) cattle heads were partially disposed from January to August, 2012 at slaughterhouses in Gwangju area, Korea. Of the 1,343 partial disposed cattle, 613 (45.6%) cattle carcasses were graded into above "Grade 1". Among partially disposed 1,171 Hanwoo heads, 613 (52.3%) cattle carcasses were graded into above "Grade 1". However, none of dairy and beef cattle carcasses were graded into above "Grade 1" out of 163 and 9 partial disposals, respectively. Of the 221 partial disposals by enteric fat necrosis, 139 carcasses were graded into above "Grade 1", and the ratio; 62.9% (P=0.001), was higher than that (50.3%) of total cattle carcass grade which is above "Grade 1". From these results, it is suggested that quality of cattle carcasses which is mainly determined by marbling might be related to the fatty lesions such as fatty liver and enteric fat necrosis.
Mycobacterium bovis 와 M. tuberculosis 감별을 위한 등온증폭법
고바라다 ( Ba Ra Da Koh ),김재명 ( Jae Myung Kim ),성창민 ( Chang Min Sung ),지태경 ( Tae Kyung Ji ),나호명 ( Ho Myung Na ),박성도 ( Seong Do Park ),김용환 ( Yong Hwan Kim ),김은선 ( Eun Sun Kim ) 한국가축위생학회 2013 韓國家畜衛生學會誌 Vol.36 No.2
Mycobacterium (M.) bovis, a member of the M. tuberculosis complex (MTC), is a re-emerging, zoonotic agent of bovine tuberculosis whose prevalence probably depends on variations in direct exposure to cattle and ingestion of raw milk. Accurate species differentiation of M. bovis and M. tuberculosis is needed to distinguish between human and zoonotic tuberculosis. This study successfully developed a loop-mediated isothermal amplification (LAMP) assay for rapid detection and differentiation of M. bovis and M. tuberculosis, however showed negative reactions in eight non-tuberculous mycobacteria (NTM) samples and ten other bacterial species. Sensitivity of this assay for detection of genomic M. bovis DNA was 10 fg/μl. And this assay successfully detected M. bovis in bovine clinical specimens. In conclusion, the LAMP assay is a simple and powerful tool for rapid detection of M. bovis in both pure bacterial culture and in clinical samples.