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나호명 ( Ho Myung Na ),배성열 ( Seong Yeol Bae ),고바라다 ( Ba Ra Da Koh ),박재성 ( Jae Sung Park ),서윤정 ( Yun Jeong Seo ),정하진 ( Ha Jin Jeong ),박자윤 ( Ja Yoon Park ),박성도 ( Seong Do Park ),김은선 ( Eun Sun Kim ),김용환 ( Y 한국가축위생학회 2016 韓國家畜衛生學會誌 Vol.39 No.2
The antibody titers against Coxiella burnetii (Q fever) among cattle raised in Gwangju area were analyzed from February to October in 2015. The prevalence of antibodies in collected bulk-tank milk from 7 dairy cattle farms was 57.1% and the seroprevalence of 210 dairy cows randomly selected from those farms was 7.1%. By age, the seroprevalence was 3.3% in less than 4 years of age, 9.0% between 4 and 7 years of age, and 28.6% in more than 8 years of age. On the other hand, the seroprevalence of the Hanwoo cattle was 0.4%. The result suggested that the antibodies against Coxiella burnetii increase as the age of the dairy cows increases and therefore, it is necessary to keep monitoring the prevalence of Q fever in Gwangju area.
나호명 ( Ho Myung Na ),배성열 ( Seong Yeol Bae ),이연이 ( Yeun Ey Lee ),박재성 ( Jae Sung Park ),박성도 ( Seong Do Park ),김은선 ( Eun Sun Kim ),김용환 ( Yong Hwan Kim ) 한국동물위생학회 2013 韓國家畜衛生學會誌 Vol.36 No.3
For the monitoring of six viral disease (CIV: canine influenzavirus, CPIV: canine parainfluenzavirus, CHV: canine herpesvirus, CPV2: canine parvovirus type 2, CCoV: canine coronavirus, CNV: canine norovirus) inspections, a total of 300 samples were collected nasal or feces from the companion dogs of animal hospital (n=98) and the abandoned dogs of animal shelters (n=202) in Gwangju, Korea. Using PCR and RT-PCR, CPV2, CPIV and CHV were detected in 55 (18.3%), 11 (3.7%), 1 (0.3%), respectively. CPV2 was highly detected in May, October and November. and CPIV was highly detected in November. But those agents were not detected the virus in March and July. Based on the results of the investigation continuous monitoring for companion and abandoned dogs will be required.
광주지역 소 생고기 선호도 및 유통단계별 세균학적 분석
나호명 ( Ho Myung Na ),배성열 ( Seong Yeol Bae ),고바라다 ( Ba Ra Da Koh ),장미선 ( Mi Sun Jang ),성창민 ( Chang Min Sung ),김지연 ( Ji Yeon Kim ),박헌규 ( Heon Gyu Park ),문용운 ( Yong Un Mun ),김용환 ( Yong Hwan Kim ) 한국동물위생학회 2012 韓國家畜衛生學會誌 Vol.35 No.4
Consumer`s preference and microbial inspections on fresh raw beef were carried out to understand the actual market status in Gwanju, Korea. Over 15 questions on questionnaire by 1,111 randomly selected respondents between April and May in 2011, results showed 65.5% positive on eating fresh raw beef, 63.8% negative on good hygiene condition of fresh raw beef, and 72.5% positive on the secure of the hygiene-safety for priority program, respectively. For microbial inspections, a total of 302 samples were collected from fresh raw beef purchased from slaughterhouse (n=122), transport (n=69) and consumer (n=81) stage, from lettuce (n=30) at consumer stage. The aerobic plate count (APC), E. coli count and food borne bacteria such as Salmonella spp., Listeria monocytogenes, Staphylococ-cus( S.) aureus and E. coli O157:H7 were tested in the samples. As results, the level of count on APC of fresh raw beef ranged 6×10(1)∼1.8×10(5) CFU/g from slaughterhouse, 2×10(2)∼8.3×10(5) CFU/g from transport stage and 1×10(2)∼4×10(5) CFU/g from consumer stage. The level of count on E. coli of fresh raw beef ranged 1∼9×10(1) CFU/g from slaughterhouse, 1∼7×10 CFU/g from transport stage and 1∼5.5×10 CFU/g from consumer stage. In total, 26 S. aureus were isolated, 10 (14.5%) from fresh raw beef at transport stage, 12 (14.8%) from fresh raw beef and 4 (13.3%) from lettuce at consumer stage. Enterotoxin of S. aureus was not detected among 26 isolates. All S. aureus isolates were typed using a DiversiLab(TM) rep-PCR system for genetic similarity test, showing over 95% of genetic relationship amon isolates.
나호명 ( Ho Myung Na ),최종욱 ( Jong Woog Choi ),박재성 ( Jae Sung Park ),이연이 ( Yeun Ey Lee ),배성열 ( Seong Yeol Bae ),박성도 ( Seong Do Park ),김은선 ( Eun Sun Kim ),김용환 ( Yong Hwan Kim ) 한국동물위생학회 2014 韓國家畜衛生學會誌 Vol.37 No.4
The purpose of this study was to determine the zoonotic diseases of stray dog and cat in Gwangju, Korea. We chose randomly 300 samples which is 265, dogs and 35, cats in the public animal shelter from March to August of 2013. The animals’ blood biochemistry values measured, and the out of normal values were 49.7% GOT, 36.3% GPT, and 78.0% GGT. The tested items were Dirofilaria immitis, Toxoplasma gondii, Brucella canis, Rabies virus. The positive results were 10% Dirofilaria immitis, 6.3% Toxoplasma gondii (antibody detected), 10% Rabies (antibody detected) but 0.0% in B. canis. The stray animals’ antibodyㆍantigen positivity take effect high from Mar.-May. Therefore, we propose that those diseases should be monitering and vaccinating in Korea.
도계장 도계의 Campylobacter 균 오염에 관한 연구
나호명 ( Ho Myung Na ),고바라다 ( Ba Ra Da Koh ),박성도 ( Seong Do Park ),김용환 ( Yong Hwan Kim ) 한국가축위생학회 2007 韓國家畜衛生學會誌 Vol.30 No.1
The present study was carried out to investigate the incidence of Campylobacter spp. from the chicken carcasses in slaughterhouse. A total of 9 strains were primarily isolated from enrichment culture and selective culture of the sample with candle and microaerophilic chamber method. Nine of Gram-negative, catalase-positive and oxidase-positive strains were further isolated by the determination of biochemical characteristics and finally identified as Campylobacter jejuni with HIP 400F and HIP 1134R primers. Therefore, this PCR method proved to be useful as a routine diagnostic test for the Campylobacter detection and confirmation of C. jejuni and C. coli in naturally contaminated poultry samples.
소, 돼지, 가금육류의 신속한 동정을 위한 TaqMan<sup>ⓡ</sup> probe를 이용한 real-time PCR 개발
고바라다,김지연,나호명,박성도,김용환,Koh, Ba-Ra-Da,Kim, Ji-Yeon,Na, Ho-Myung,Park, Seong-Do,Kim, Yong-Hwan 한국동물위생학회 2012 韓國家畜衛生學會誌 Vol.35 No.3
Species-specific $TaqMan^{(R)}$ probe-based real-time PCR assays were developed for detection of beef, pork, chicken, duck, goose and turkey. The primer and probe sets used in this study were designed to be complementary to fibroblast growth factor (FGF) for cattle and pig, mitochondrial NADH dehydrogenase (ND) subunit 3 and ND2 for chicken and duck, 12S rRNA for goose and turkey, respectively. As internal positive control we used conserved region in the ribosomal 18S RNA gene to ensure the accuracy of the detection of target DNA by real-time PCR. We confirmed that real-time PCR assays with the primer and probe sets were positive for cattle, pig and chicken intended target animal species with no cross-reactivity with other non-target animal species. Only >50 ng DNA of beef show cross-reactivity in the determination of duck. Using species-specific primer and probe sets, it was possible to detect amounts of 0.1 ng DNA of cattle and pig, 1.0 pg DNA of chicken, duck and turkey, and 0.1 pg DNA of goose for raw samples, respectively. The detection limits were 0.1 ng DNA of cattle, 1.0 ng DNA of pig and 1.0 pg DNA of chicken for DNA mixtures (beef, pork and chicken) extracted from heat-treated ($121^{\circ}C$/5 min) meat samples. In conclusion, it can be suggested that the $TaqMan^{(R)}$ probe-based assay developed in this study might be a rapid and specific method for the identification of meat species in raw or cooked meat products.
돼지고기 제품 내 닭고기 검출을 위한 TaqMan<sup>®</sup> real-time PCR의 적용
고바라다,김지연,나호명,박성도,김용환,Koh, Ba-Ra-Da,Kim, Ji-Yeon,Na, Ho-Myung,Park, Seong-Do,Kim, Yong-Hwan 한국동물위생학회 2013 韓國家畜衛生學會誌 Vol.36 No.3
Many consumers are increasingly concerned about the meat they eat, and accurate labelling is important due to public health, economic and legal concerns. Meat species adulteration is a common problem in the retail markets. In this study, a TaqMan$^{(R)}$ quantitative real-time polymerase chain reaction (PCR) assay was applied for its ability to quantify chicken meat, which was not indicated on the label, in 79 commercial pork products (ham, sausages, bacon and ground meat) producted by 10 different manufacturers. The amplification efficiency was 82.05% and the square regression coefficient ($R^2$) was 0.995. PCR results showed that 38.6% of ham samples, 50.0% of sausages samples, and 50.0% of ground meat samples were contaminated with chicken residuals, while the bacon samples were not contaminated with chicken residuals. Only twelve pork products of one of the manufacturers were in accordance with indicated in their labels. The PCR assay reported in this work could be particularly useful in inspection programs to verify the food labelling of commercial processed meats and to gain consumers' trust.
동물원 사자의 Clostridium perfringens에 의한 장독혈증 감염증례
김용환 ( Yong Hwan Kim ),나호명 ( Ho Myung Na ),박성도 ( Sung Do Park ),고바라다 ( Ba Ra Da Koh ),김태순 ( Tae Sun Kim ),윤병철 ( Byeong Chel Yoon ),최종욱 ( Jong Woog Choi ),이삼수 ( Sam Soo Lee ) 한국가축위생학회 2005 韓國家畜衛生學會誌 Vol.28 No.3
A 3-year-old male lion at Gwangju Uchi Zoo presented for acute onset of haemorrhagic diarrhea and died. The lion showed reddening of the anus as the cause of haemorrhagic enteritis. Necropsy revealed a severe haemorrhagic colitis. Grossly, lesions included icterus, excess pericardial fluid, dark kidneys, and an enlarged, friable liver. The intestines were flaccid, thin-walled, dilated, and gas-filled. The spleen was enlarged and pulpy because of congestion. Most of organs were rapidly postmortem autolysis. Histopathologically, the intestines were edema and transient leukocyte infiltration of the lamina propria, followed by necrosis. Especially of the intestinal submucosa was edematous, haemorrhagic, or filled with leukocytes. The crypts remained intact or dilated. C perfringens was isolated from a lion at bloody feces, and identified C perfringens type A, confirming the presence of C perfringens α-toxin by PCR. These results were suggested that the case were diagnosed as enterotoxicosis in the lion. More studies are needed on lion enterotoxemia, especially of its etiopathogenesis, in order to develop more efficient prevention for this disease.
Mycobacterium bovis 와 M. tuberculosis 감별을 위한 등온증폭법
고바라다 ( Ba Ra Da Koh ),김재명 ( Jae Myung Kim ),성창민 ( Chang Min Sung ),지태경 ( Tae Kyung Ji ),나호명 ( Ho Myung Na ),박성도 ( Seong Do Park ),김용환 ( Yong Hwan Kim ),김은선 ( Eun Sun Kim ) 한국가축위생학회 2013 韓國家畜衛生學會誌 Vol.36 No.2
Mycobacterium (M.) bovis, a member of the M. tuberculosis complex (MTC), is a re-emerging, zoonotic agent of bovine tuberculosis whose prevalence probably depends on variations in direct exposure to cattle and ingestion of raw milk. Accurate species differentiation of M. bovis and M. tuberculosis is needed to distinguish between human and zoonotic tuberculosis. This study successfully developed a loop-mediated isothermal amplification (LAMP) assay for rapid detection and differentiation of M. bovis and M. tuberculosis, however showed negative reactions in eight non-tuberculous mycobacteria (NTM) samples and ten other bacterial species. Sensitivity of this assay for detection of genomic M. bovis DNA was 10 fg/μl. And this assay successfully detected M. bovis in bovine clinical specimens. In conclusion, the LAMP assay is a simple and powerful tool for rapid detection of M. bovis in both pure bacterial culture and in clinical samples.
광주지역 동물보호소내 유기견의 개심장사상충과 개 브루셀라병 감염 실태조사
고바라다 ( Ba Ra Da Koh ),나호명 ( Ho Myung Na ),장미선 ( Mi Sun Jang ),김지연 ( Ji Yeon Kim ),박성도 ( Seong Do Park ) 한국가축위생학회 2007 韓國家畜衛生學會誌 Vol.30 No.1
This study was conducted to investigate the prevalence of canine heartworm infections, canine brucellosis and hematologic values from 153 free roaming dogs in the area of Gwangju city from March to November 2006. Nineteen (12.4%) of 153 samples tested with modified Knott`s technique showed positive reaction for microfilariae. Polymerase chain reaction using specific primers for D immitis amplified the expected product from all samples of 19 microfilaremic canine blood samples as determined by the modified Knott`s test for microfilariae. The seasonal infection rates of microfilariae were higher in the spring season (10/19, 52.6%) than in the other seasons. The major hematological findings in microfilaremic dogs were mild leukocytosis and mild monocytosis. A total of 100 dogs randomly selected from 153 free roaming dogs were negative for canine brucellosis by serological test using immunochromatographic antibody test kit.