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발육중인 생쥐 하악 과두에서 연골 및 골의 특이 유전자 발현
지국섭,윤영주,박주철,김광원 대한치과교정학회 2004 대한치과교정학회지 Vol.34 No.2
하악과두 연골이 발생하고 분화하는 과정에서 나타내는 특성을 규명하기 위하여, 발생 16, 18일과 출생 1일, 5일, 10일, 20일 및 30일 후의 ICR생쥐의 하악과두를 형태학적으로 분석하고, 생쥐 Ⅰ형, Ⅱ형, X형 교원질, Indian hedgehog(IHH) 및 BMP-4 등의 mRNA 발현을 in-situ hybridization 방법으로 연구하였다. 1. 생쥐 Ⅰ형 및 Ⅱ형 교원질 mRNA는 하악과두의 발생 및 성장과정에서 모두 발현되었다. Ⅰ형 교원질 mRNA는 휴지층과 증식층의 상부에서 관찰된 반면 Ⅱ형 교원질은 휴지층과 증식층 그리고 비대연골층의 상부에서 관찰되었다. 2. 하악과두 연골은 성장에 따라 비대연골층이 계속 증가하는 소견을 보였으며, 비대 연골층의 세포들은 특징적으로 X형 교원질 mRNA의 발현을 보였다. 3. BMP-4 mRNA는 하악과두 연골 원기와 골화중인 하악골체에서 모두 발현되었다. 4. IHH mRNA는 하악과두의 발생과정에서 증식 연골층의 하부와 비대연골층의 상부에서 선택적으로 관찰되었다. It has not been elucidated whether the initiation of condylar development of the mandible is related with the periosteum of the mandible, or if it derives from a separate programmed blastema not related with the mandible. Also, although the mandibular condylar cartilage is known to promote growth, few studies have dealt with molecular-biologic mechanisms such as the expression of specific genes according to the differentiation of the mandibular condyle. To elucidate the unique cellular characteristics, development, and differentiation process of the mandibular condyle, an examination of expressions of genes characteristic of cartilage and bone were carried out using RT-PCR and mRNA in situ hybridization. 1. Type?collagen mRNA was detected with type Ⅱ collagen mRNA in the differentiation and growth process of the cartilage of the mandibular condyle. Type Ⅱ collagen mRNA was demonstrated in the whole resting and upper part of the proliferative zone, whereas type Ⅱ collgen mRNA was observed in the resting, proliferative and upper hypertrophic cartilage zone of the mandibular condyle. 2. The condylar cartilage rapidly increased in size due to the accumulation of hypertrophic chondrocytes as characterized by the expression of type Ⅱ collagen mRNA during postnatal development. 3. BMP-4 mRNA was present in the anlage of the future condylar process and also in the ossifying mandibular body. 4. IHH mRNA was limited exclusively to the lower part of the proliferative zone and the upper part of the hypertrophic cartilage zone during condylar development. These findings were different from those in the growth-plate cartilage of the long bone, indicating a characteristic feature of the differentiation of the chondrocytes in the condylar cartilage present in prenatal and postnatal development. Furthermore, it was also suggested that chondroblasts of condylar cartilage rapidly differentiate into hypertrophic chondrocytes with increased functional Load force such as muscle activity and mastication.
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지국섭,김광원 대한치과교정학회 1996 대한치과교정학회지 Vol.26 No.2
이 연구는 악안면 영역의 심미적 개선에 필요한 미소의 형태에 대한 기준을 설정하기 위하여 시행하였다. 성인 정상교합자 62명(남자:30명, 평군연령;22.7세, 여자;32명, 평균연령;21.8세)을 대상으로 안정위시와 미소시의 얼굴 정면 사진을 촬영하였고, 미소시 입술의 형태변화, 그리고 입술과 치아와의 관계를 계측, 분석하였다. 이 연구로부터 얻어진 결과는 다음과 같다. 1. 하순 상연의 만곡과 상악 절단연과의 평행관계인 smile line ratio는 0.09이었고, buccal corridor ratio는 0.63, smile symmetry ratio는 0.96이었다. 2. 미소시 양 구각주 간의 거리는 안정위시 구각부 간 거리의 1.31배였으며, 얼굴 폭의 0.48배였다. 3. 미소시 상순의 수직길이는 안정위시 길이의 0.69배였고, 하순의 수직길이는 안정위시 길이의 0.96배였다. 4. 상악 전치의 노출량은 9.96mm이었고, 상악 전치의 노출은 미소시 상순의 수직길이의 변화율이 었고, 미소시 구각부 길이의 비, buccal corridor ratio등과 관계 깊었다. This study was performed to mark the criteria of the eathetic smile that was necessary to improve the esthetic problem in oral maxillo-facial region. The facial straight photographs of 62 adults(30 males, 32 females : 19-24 years old) in resting position and during smile were taken. The measurements and proportion of lip-teeth relationship during smile were statistically analized with photogrammetry. The following results were obtained: 1. In the evaluation of the change of lips, smile line ratio was 0.93, buccal corridor ratio was 0.63, and smile symmetry ratio was 0.96. 2. The width of mouth during smile was 1.31 times of the width in the resting position and 0.48 times of face width. 3. The upper lip height during smile was 0.69 times of the height in the resting position and the lower lip height during smile was 0.93 times of the height in the resting position. 4. The mean exposed lenght of upper central incisor was 9,.96mm. Maxillary incisor exposure was significantly correlated with the upper lip change ratio, mouth width changer ratio, and buccal corridor ratio.
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김경님,지국섭,김중한,이승미,김미애,박형수,임한호,윤영주,김광원,박관수 朝鮮大學校 口腔生物學硏究所 2000 口腔生物學硏究 Vol.24 No.2
Even though congential missing of mandibular incisors is lower incidence than that of maxillary lateral incisors of premolars, it makes orthodontic treatment complicated, and then clinical orthodontists should be familiar with treatment alternatives. The treatment techniques used in resolving that cases depend on a myriad of diagnostic criteria such as dental, skeletal and aesthetic considerations. Be based on this consideration, the orthodontists must decide whether to retain or open space for prosthodontic treatment or to closure space with potential excessive overbite of the anterior teeth or to extract maxillary teeth for tooth material balance. The following case reports document three patients with one or two congenitally missing mandibular teeth who were treated by opportunely selected treatment modality.
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조명훈,김동훈,김중한,지국섭,박성만,윤영주,김광원 朝鮮大學校 口腔生物學硏究所 2000 口腔生物學硏究 Vol.24 No.1
The aim of the investigation was to evaluate the validity of the predictions of a computerized video imaging prediction program(QuickCeph Image Pro^(R)) regarding dentoskeletal and soft tissue profile changes after various orthognathic surgery. With the advance of refined surgical procedures, such as the LeFort I osteotomy and the bilateral sagittal split osteotomy, a combined orthodontic/orthognathic surgical approach for the treatment of dentofacial deformities has become routine. And video imaging is a suitable tool for use in patient presentation. education. and subsequent treatment planning. Video imaging techniques have improved dramatically and are becoming increasingly important to clinicians and patients for communication and visualization during treatment planning sessions. With a little effort, patience, and accurate record-taking. the clinician can use video imaging to enhance the surgical-orthodontic treatment planing and patient communication processes. In this study, we compared the real postsurgical profile and predicted profile b use of machintosh-based program((QuickCeph Image Pro^(R)), and evaluated accuracy of the QuickCeph Image Pro^(R). The result of this study, along with conflicting evidence found in the current literature, lead us to believe that on acceptable soft-to-hard-tissue ration has yet to be developed to accurately predict the postsurgical posture of the profile. The need for various soft-to-hard-tissue algorythmes and accurate soft-to-hard-tissue ratios is imperative to improve the accuracy of video imaging in predicting soft tissue profiles following orthognathic surgery. Additionally, it is recommended that a greater understanding of the nature of the facial soft tissue and its response to changes of the underlying hard tissue bo attained.
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황미선,기윤경,차승라,오창근,지국섭,김동훈,박미숙,김정호,윤영주,김광원 朝鮮大學校 口腔生物學硏究所 1999 口腔生物學硏究 Vol.23 No.2
In most individuals, the permanent teeth will be erupt uneventfully and replace their primary successors. However, sometimes teeth fail to erupt. Most of these unerupted teeth are diverted or angulated aberrantly and eventually lose their potential to erupt. It is referred to as impacted teeth. These teeth are not hopeless; it is possible to use the remaining teeth as anchorage, surgically uncovered the impacted tooth, and orthodontically move the tooth into the arch. The specific surgical procedure and the orthodontic mechanics, however, will vary depending upon the type of tooth and its position relative to the remaining erupted teeth. This study identifies the most commonly impacted teeth and discusses the causes of impaction and describer the various surgical procedures to uncover these teeth, and illustrates the orthodontic mechanics used to erupted the teeth into proper alignment and occlusion.
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모신엽,기윤경,차승라,오창근,지국섭,김동훈,박미숙,김정호,윤영주,김광원 朝鮮大學校 口腔生物學硏究所 1999 Oral Biology Research (Oral Biol Res) Vol.23 No.2
Dental care providers are routinely exposed to multitude of bacteria, viral, fungal and other microbial pathogens during patient treatment. Adequate attention to prevention of cross-contamination in the dental operatory has largely been ignored by orthodontists. This has occurred because orthodontic procedures are usually nontissue invasive. Unfortunately, orthodontists have the second highest incidence of hepatitis B among dental professionals. The purpose of this study is to empathize th infection control through the visualization of the bacteria in contaminants. We certified that there are high possibility of contact with the infection source during treatment. Our goals should be to reduce the number of pathogenic organisms to a level at which our own body resistance may prevent infection and to break the circle of infection by elimination cross-contamination. We are not attempting to maintain the sterility of the instruments. We are sterilizing to break the pathway of contamination between patient to patient, patient to doctor.
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