Gametophyte Propagation and Sporophyte Formation of Asian Chain Fern [Woodwardia japonica (L. f.) Sm.] Under Various Medium Conditions In Vitro and Ex Vitro

조주성,장보국,박경태,이하민,이철희 한국자원식물학회 2019 한국자원식물학회지 Vol.32 No.6

This study investigated a suitable method that could be applied for Asian chain fern [Woodwardia japonica (L. f.) Sm.] to propagate gametophytes and promote sporophyte formation. The gametophytes used in all experiments were obtained from germinated spores in vitro and were subcultured at 8-week intervals. The most appropriate media for gametophyte propagation was identified by culturing 300 ㎎ of gametophyte in Murashige and Skoog (MS) basal medium (1/8, 1/4, 1/2, 1, 2), and Knop medium for 8 weeks. As a result, fresh weight of the gametophyte was increased by 56.7-fold on MS medium. Moreover, antheridium formation as well as gametophyte growth was improved on MS medium, especially. To improve the sporophyte formation ex vitro, 1.0 g of gametophyte was ground with distilled water and spread on eight combinations onto four different culture mediums, such as bed soil, peat moss, perlite and decomposed granite. Then generation and growth of sporophytes were investigated after cultivation for 10 weeks. As a result of this experiment, peat moss had a promotive effect of sporophyte formation at single-use and mixed culture soils. In particular, a mixture of bed soil, peat moss and perlite in a 1:1:1 ratio (v/v/v) led to the accelerated formation (782.5 ea/pot) and the frond growth of sporophytes. This included increases in length and width of fronds. However, promotive effect of gametophyte growth and sporophyte formation was not found at single-use and treatment with high ratio of bed soil.

바늘꽃과 2종의 환경조건 및 화학적 처리에 따른 종자 발아 특성

조주성 충북대학교 농업과학기술연구소 2019 農業科學硏究 Vol.35 No.2

본문참고

Effect of germination and water absorption on scarification and stratification of kousa dogwood seed

조주성,이철희 한국원예학회 2018 Horticulture, Environment, and Biotechnology Vol.59 No.3

Cornus kousa (kousa dogwood), used as a landscape tree and a source of food and medicine, is difficult to propagate from seeds owing to dormancy. The present study was conducted to develop an effective method of seed propagation and identify the seed dormancy type. Kousa dogwood seeds were classified as presenting physical dormancy (PY) and physiological dormancy (PD) from an immature embryo. Seeds were contained within an impermeable endocarp that conferred PY and had an undifferentiated embryo that grew and differentiated after scarification and cold moist stratification, leading to improved germination. To break PY + PD, four replicates of 50 seeds (3200 in total) for each treatment were prepared by scarification and cold moist stratification. These methods combined improved moisture absorption and germination more than either treatment alone. In particular, the addition of cold moist stratification treatment to scarified seeds could effectively induce embryo development. By contrast, untreated seeds showed low germination rate of less than 1% at 30 °C with continuous light. Sulfuric acid (H2SO4) at different concentrations and durations was used to uniformly wound the endocarp of seeds. Treatment with 80% H2SO4 for 10 min resulted in the highest germination rate (68.6%) and moisture content (65.7%), which were higher than those following polishing with a brushing machine for 5–40 min. Longer treatment with high concentrations of H2SO4 (80 and 100%) caused the acid to infiltrate the seed and inhibit germination by damaging internal tissues, while low concentrations (40 and 60%) did not affect germination rates even after treatment for more than 10 min. To obtain maximum germination, concentration and treatment duration of sulfuric acid should be carefully controlled.

부적응 병사를 위한 인지행동치료 및 수용전념치료 집단상담 프로그램 개발 및 효과 검증

조주성,최재광,강요한,유한별,송원영,Cho, Ju Sung,Choi, Jaegwang,Kang, Yohan,Yu, Han Byeol,Song, Wonyoung 중소기업융합학회 2020 융합정보논문지 Vol.10 No.11

본 연구는 국군 장병들의 부적응 문제 해결을 위한 집단상담 프로그램을 개발하고 프로그램의 효과를 검증하기 위해 진행하였다. 정서 및 행동문제를 경험하는 군 장병을 위한 12회기 CBT 및 ACT 프로그램을 개발하고 충남권 소재 육군 부대 장병을 대상으로 CBT, ACT 각 2집단씩 주 1회 프로그램을 실시해 효과성을 검증하였다. 결과분석을 위해 사전-사후 성인용 행동평가 척도(ASR)을 시행하였고, 기술통계분석, 교차분석, 대응t검증, 독립t검증을 실시하여 분석하였다. 나타난 결과는 다음과 같다. 첫째, 집단 간 사전 동질검사 결과 두 집단은 인구통계학적특성과 주요변인의 특성이 동질한 것으로 나타났다. 둘째, 사전-사후 비교검증 결과 CBT집단과 ACT집단 모두 문제행동총점 및 내재화, 외현화의 변화가 유의하였다. 셋째, CBT집단과 ACT집단의 사전-사후 검사의 차이는 나타나지 않았다. 본 연구는 군부대 내 부적응 문제에 개입한 점, 두 이론간의 효과성을 비교했다는 점에서 의미가 있으며, 향후 연구를 위한 방법론 및 연구 대상 확대를 제안하였다. This study was conducted to develop a group counseling program to solve the problem of maladjustment of military personnel and to verify the effectiveness of the program. The 12 session CBT and ACT program for military soldiers experiencing emotional and behavioral problems was developed and the effectiveness was verified by conducting a program once a week for soldiers in the army units based in South Chungcheong Province. Adult Self Report scale(ASR) was implemented for pre-post test and analysis, and descriptive statistical analysis, cross-analysis, paired sample t-test, and independent sample t-test were performed to analyze the results. The results indicated are as follows: First, the preliminary homogeneity test between groups showed that the two groups had homogeneous demographic characteristics and the characteristics of the variables. Second, as a result of the pre-post test verification, both CBT and ACT groups showed significant changes in problem behavior totals, internalizations, and externalizations. Third, there was no difference between the pre-post-examination of the CBT group and the ACT group. This study is meaningful in that it was involved in the maladjustment problem within the military unit, and compared the effectiveness between the two theories, and proposed the study of control groups and further studies on more diverse and diverse subjects that were not implemented under the circumstances of the unit.

자생 끈끈이주걱(Drosera rotundifolia L.) 종자의 휴면과 발아특성

조주성,이철희 한국자원식물학회 2016 한국자원식물학회지 Vol.29 No.5

This research was performed to develop mass propagation method of round-leaved sundew (Drosera rotundifolia L.) for improving horticultural usefulness. Seeds were collected around Hwaseong-si, Gyeonggi-do in October of 2011 and experimented upon while being dry-stored at 4 ± 1.0℃ (darkness). Seed length of a major and minor axis were 1.58 ± 0.060 and 0.21 ± 0.016 ㎜, respecrively, and weight of 1,000 seeds was 6.24 ± 0.172 ㎎. Seeds were thus classified as ‘dwarf seeds’. Regarding the dormancy type, since round-leaved sundew seeds were dormancy broken and germinated at 20~30℃ under the light condition after wet-chilling treatment for 12 weeks, seeds were estimated to have physiological dormancy. Germination conditions of dormancy broken seeds were found to be 20℃ and light condition (54.7%), but germination decreased at higher temperature. Percent germination (PG), germination energy (GE), mean germination time (MGT) and T50 were effectively improved by chemical treatment such as GA3 200 ㎎/L + kinetin 20 ㎎/L and wet-chilling treatment for 14 weeks. In conclusion these optimal conditions were thought to be a useful method for raising seedling uniformly.

난쟁이바위솔의 종자발아를 촉진하는 온도, 광 및 화학적 처리

조주성,정정학,김수영,이철희 한국화훼산업육성협회 2014 화훼연구 Vol.22 No.2

본 연구는 식물구계학적 특정식물종 II급에 해당하는 난쟁이바위솔의 종자 번식법 개발을 위해 수행되었다. 종자의 형태적 특성은 크기가 1.10x0.21mm, 1,000립 중이 496.0±0.21mg인 갈색의 미립종자였다. 종자의 함수율은 24시간 만에 8.8%에서 39.8%로 급속히 증가하여최대함수율을 보였다. 온도 및 광조건에 따른 발아율은20οC, 명조건에서 가장 높았으며, GA3와 KNO3용액에 24시간 처리함으로써 발아율과 발아세가 매우 향상되었다.특히 GA3 처리구는 10~100mg•L-1의 농도에서 발아율이99.0% 이상으로 매우 우수였으며, KNO3는 5mM 처리구에서 발아율이 81.3%로 가장 높았다. This study was carried out to establish seedpropagation methods of Meterostachys sikokiana (Makino)Nakai, one of the specific plant species for the floral region.Observation of seed morphology characters showed wrinkledsurface and elliptical shape, indicating brown, and fineseeds. Seed size ranged 1.10x0.21mm, weight of thousandseeds was 496.0±0.21mg. Moisture contents of seeds,increased rapidly by water-soaking treatment, recordingmaximum moisture contents after 24 hours. Percent ger-mination of seeds was higher under the light than darkcondition. Seed germination was the best at 20οC underthe light condition among temperature and light conditionstreated. Percent germination and germination energy wasgreatly improved by soaking in GA3 and KNO3 solutions for 24hours. Especially, 10~100mg•L-1 GA3 treatment resulted inthe highest germination rate as 99.0% and above. In caseof KNO3 treatment, the highest germination rate was 81.3%in 5mM solution.

설앵초의 종자 휴면과 발아특성

조주성,이철희 한국자원식물학회 2017 한국자원식물학회지 Vol.30 No.4

This research was performed to develop mass propagation method of Alpine modest primrose (Primula modesta var. hannasanensis T.Yamaz.) for improving the conservation and utilization of the species. Seeds were collected on August 2011 in Jeju-city and well-selected seeds were dry-stored at 4 ± 1.0℃. Seed size ranged 0.44 ± 0.07 × 0.61 ± 0.04 ㎜, and weight of 1,000 seeds was 51.78 ± 0.021 ㎎. Thus the seed was classified as ‘dwarf seeds’. As the result of dormancy characteristics, moisture content of freshly matured seeds increased rapidly by water-soaking treatment and seeds did not germinate at 20℃ for 4 weeks under the light condition. Therefore, seeds were estimated to have physiological dormancy. Germination conditions of dry-stored seeds were found to be 20℃ and light condition, and seeds did not germinated under dark condition regardless of all temperature regimes. Therefore, Alpine modest primrose seed was considered as photoblastic type. Percent germination (PG) and germination energy (GE) were greatly improved by soaking the seeds in GA3 and kinetin for 24 hours. Especially, 200~500 ㎎/L GA3 treatment resulted in the highest PG (95.5%) and GE (98.3%). So, chemical treatment such as GA3 was thought to be a useful method for raising seedling uniformly. 본 연구는 설앵초의 종 보존과 활용성 증대를 위해 종자를이용한 대량 번식법을 개발하고자 수행되었다. 종자는 2011년제주도 일대에서 채종하여 정선한 다음 4 ± 1.0℃에서 건조 상태로 저장하면서 연구에 사용하였다. 종자의 크기는 0.44 ± 0.07 × 0.61 ± 0.04 ㎜, 1,000립중은 51.78 ± 0.021 ㎎이었으며, 미세종자로 분류되었다. 설앵초 종자의 휴면특성은 생리적휴면(physiological dormancy)성을 갖는 것으로 판단되었다. 한편 종자는 광조건에서 발아가 되는 광발아성 종자로, 적정온도는 20°C로 나타났다. GA3는 조자의 휴면타파 및 발아력 향상에 크게 효과가 있었는데, 농도가 높아줄수록 그 효과가 크게 나타났다. 특히 GA3 200~500 ㎎/L 처리구에서 발아율 및 발아세가 90% 이상으로 매우 높게 조사되었다.

The Effects of Environmental Conditions and Chemical Treatments on Seed Germination in Astilboides tabularis (Hemsl.) Engl.

조주성,정정학,이철희 한국원예학회 2016 원예과학기술지 Vol.34 No.3

Tabulis [Astilboides tabularis (Hemsl.) Engl.] has the potential for becoming a useful horticultural crop. This study was conducted to classify the seed dormancy types and to identify the germination conditions that improve the horticultural usefulness of this plant. We found that A. tabularis (Hemsl.) Engl. produced undifferentiated embryos and had a low germination rate of under 50%. In addition, water submersion led to moisture absorption. Therefore, A. tabularis seeds were designated as morphologically dormant. The germination rate was highest (86.3%) at 30°C under light conditions. Higher temperatures generally led to an acceleration of the germination process, regardless of light condition. To improve the germination rate, seeds were submerged in various concentrations of growth regulators, such as GA3 and kinetin, as well as minerals such as KNO3 and KCl. An analysis of germination characteristics at 30°C under light conditions revealed that treatment with 200 and 500 mg·L-1 GA3 led to an excellent germination rate of 97.0%. Treatment with 100 mg·L-1 GA3 led to a better germination rate than the control as well. However, treatment with kinetin, KNO3, and KCl had no effect on germination, regardless of concentration.

멸종위기 야생식물 삼백초의 종자발아에 미치는 화학적 처리의 영향

조주성,이철희 한국자원식물학회 2016 한국자원식물학회지 Vol.29 No.4

This research was performed to develop seminal propagation method of Saururus chinensis (Lour.) Baill. by conducting a rigorous germination study. Well-selected seeds were dry-stored at 4 ± 1.0℃ during the experiment. To study dormancy type, non-stored seeds were analyzed by embryo observation, germination test and detecting for any difficulties in seed coat to absorb moisture. Then to improve germination, seeds were submerged for 24 hours in a solution of varying concentrations containing one of plant growth regulators and minerals. According to research, fleshly matured seeds had an undifferentiated embryo and had a low germination rate below 5%. In addition, water submersion led to moisture absorption, embryo in the seeds grew and germinated so it was deemed morphophysiological dormant seeds. Percent germination (PG) and germination energy (GE) was greatly improved by soaking in plant growth regulators and minerals for 24 hours. Especially, 500 ㎎/L GA3 treatment resulted in the highest GE as 46.1%. KNO3 meaningfully improved PG (54.3∼57.7%) at 10∼20 mM but effect of minerals on germination acceleration as GE were negatively impacted in all concentrations.

등수국의 종자발아에 미치는 몇 가지 요인

조주성,정정학,김수영,이주영,이철희 한국자원식물학회 2014 한국자원식물학회지 Vol.27 No.5

This study was carried out to establish seed propagation method of Hydrangea petiolaris Siebold & Zucc., one of the specific and protected plant species for the floral region. Seed size ranged 1.36 × 0.84 ㎜, weight of thousand seeds was 2.3 ± 0.02 ㎎. Observation of seed morphology characters showed wrinkled surface and elliptical shape, indicating amber color and fine seeds. Moisture contents of seeds, increased rapidly by water-soaking treatment, recording maximum moisture contents (31.9%) after 48 hours. Percent germination of seeds was higher under the light than dark condition. Seed germination was the best at 25℃ under the light condition (78.0%) among temperature and light conditions treated. Percent germination, germination energy and T 50 was greatly improved by soaking in GA 3 solutions for 48 hours. Especially, 100∼ 500 ㎎·L -1 GA 3 treatment resulted in the highest germination rate as 90.0% and above. 본 연구는 식물구계학적 특정종 III 등급이며, 국외반출 시 승인이 필요한 보호종인 등수국의 종자 번식법을 개발하기 위해수행되었다. 종자의 크기는 1.36×0.84 ㎜이며, 1,000립 중은2.3 ± 0.02 ㎎이었다. 종자는 타원형으로 표면에 주름이 있었으며, 황색의 미립종자였다. 침지처리에 의해 종자의 함수율은 48시간 만에 3.9%에서 31.9%로 급속히 증가하여 최대 함수율을보였다. 온도 및 광조건에 따른 발아율은 25°C, 명조건에서 가장 높았으며(78.0%), GA3 용액에 48시간 처리함으로써 발아율,발아세 및 T50이 매우 향상되었다. 특히 GA3 처리구는 100∼500㎎·L⁻¹의 농도에서 발아율이 90.0% 이상으로 매우 우수였으며발아세와 T50을 무처리에 비해 향상시킬 수 있었으므로, GA3는등수국 종자의 발아력을 효과적으로 향상시킬 수 있는 적절한화학적 처리방법으로 판단되었다