http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
식용 빅벨리 해마(Hippocampus abdominalis) 유래 단백질 가수분해물 의 항산화와 항고혈압 효능
제준건,김현수,이효근,오재영,Lei Wang,노섬,전유진 한국수산과학회 2019 한국수산과학회지 Vol.52 No.2
Seahorses have long been used as ornamental and medicinal products. The sea horse Hippocampus abdominalis has a beautiful color and unique shape and is also used for ornamental purposes and as a traditional medicine in China. This study examined the value of H. abdominalis as a health functional food or food additive. H. abdominalis was hydrolyzed using seven proteases: flavourzyme, neutrase, alcalase, trypsin, kojizyme, pepsin and protamex. The yields of all of the enzyme hydrolysates were higher than that of the aqueous extract. Of the enzymatic hydrolysates, seahorse Protamex hydrolysate (SHP) gave the highest yield and had excellent antioxidant and angiotensin-I converting enzyme inhibitory activities. It protected Vero cells against oxidative by 2,2-azobis-(2-amidinopropane) dihydrochloride (AAPH) and antihypertension in Spontaneously Hypertensive Rats. This study attempted to demonstrate H. abdominalis as a health functional food or food additive in the future.
연육 제조과정에서 발생하는 어피 부산물의 활용을 위한 Cosmeceutical 활성 평가
오재영,이효근,제준건,왕뢰,김현수,전유진 한국수산과학회 2020 한국수산과학회지 Vol.53 No.3
This study investigated the cosmetic effects of enzymatic hydrolytes of an aquatic by-product, fish skin. The skins ofolive flounder Paralichthys olivaceus (PO) and Alaska pollock Gadus chalcogrammus (AP) were hydrolyzed usingpepsin, Alcalase, and Protemax. Three enzymatic hydrolytes were obtained and the inhibitory effects of these hydrolyteson the aging-related enzymes tyrosinase, elastase, and collagenase were determined. The results indicated thatthe pepsin hydrolytes of PO and PA had stronger activities than the other hydrolytes. PO and PA also significantlyreduced the intracellular reactive oxygen species levels in and improved the viability of H2O2-treated Vero cells;decreased nitric oxide production by and increased the cell viability of lipopolysaccharide-treated RAW 264.7 cells;and reduced intracellular reactive oxygen species levels and improved the viability of ultraviolet B irradiated HaCaTcells and human dermal fibroblasts. Furthermore, PO and PA remarkably reduced the intra- and extracellular melanincontents of alpha-melanocyte-stimulating hormone-stimulated B16F10 cells. These results demonstrate that PO andPA have potential for use in the cosmetic industry.
식용 해조류 톳(Hizikia fusiformis) 유래 항산화 활성분획물의 산화적 손상이 유도된 인간피부섬유아세포 보호 효과
Yong Ri Cui,김현수,제준건,Lei Wang,오재영,Liu Jia,전유진 한국수산과학회 2019 한국수산과학회지 Vol.52 No.1
We investigated the protective effects of antioxidant fractions from a 70% ethanolic extract of Hizikia fusiformis in human dermal fibroblasts (HDFs). Powdered H. fusiformis was extracted with 70% ethanol and then partitioned into three fractions according to polarity using n-hexane (HFH), chloroform (HFC), and ethyl acetate (HFEA). Antioxidant activity was observed in HFEA at 0.66 mg/mL based on the half maximal inhibitory concentration (IC50) of 1,1-diphenyl-2-picrylhydrazyl (DPPH), and at 0.24 mg/mL based on alkyl radical scavenging. The protective effects of the HFEA antioxidant fraction against 2,2-azobis-(2-amidinopropane) dihydrochloride (AAPH)-damaged HDFs and the expression of Type I procollagen in HDFs were examined. HFEA caused the proliferation of HDFs with and without AAPH treatment and protected against AAPH damage to HDFs in a dose-dependent manner (50-200 μg/mL). This implies that the antioxidant properties of the fractions depended on their proliferative and protective effects. The HFEA antioxidant fraction had significant effects and caused the dose-dependent expression of Type I procollagen, an important anti-wrinkle protein, in HDFs. In conclusion, antioxidant substances in H. fusiformis were found in the ethyl acetate fraction, and the resulting HFEA may have cosmetic applications.
남아프리카공화국 갈조류 Ecklonia maxima 줄기의 항산화 활성평가
최예지(Ye-Ji Choi),김현수(Hyun-Soo Kim),제준건(Jun-Geon Je),Lei Wang,전유진(You-Jin Jeon) 한국해양바이오학회 2019 한국해양바이오학회지 Vol.11 No.1
In the present study, the alcoholic and enzymatic extracts of Ecklonia maxima were prepared and their antioxidant activities were evaluated via the free radical scavenging activities. The lyophilized seaweed powder was extracted by 70% ethanol and the resultant extract was obtained, named as the alcoholic extract of E. maxima stem (EMSE) obtained. The residue after EMSE preparation was continuously by Celluclast-assistant extraction and then the obtained enzymatic extract was identified as polysaccharides-rich extract of E. maxima stem (EMSP). The results indicated that the extraction yields of EMSE and EMSP were 2.18% and 5.43%, respectively. In addition, the free radical scavenging activities of EMSE and EMSP were determined using an electron spin resonance (ESR) spectrometer. EMSE containing 5.36% of polyphenol and 1.56% of polysaccharide scavenged DPPH and alkyl radicals at the IC<SUB>50</SUB> values of 0.48 and 0.63 mg/mL, respectively. EMSP containing 0.33% of polyphenol and 41.58% of polysaccharide showed at the IC<SUB>50</SUB> values of 0.33 and 0.55 mg/mL, respectively. These results demonstrate that EMSP containing a high amount of polysaccharides possesses a strong free radical scavenging activity which might be a potential ingredient in pharmacological and cosmeceutical industries.
김영상(Young-Sang Kim),Thilina U. Jayawardena,Lu Yu An,제준건(Jun-Geon Je),D. P. Nagahawatta,N. M. Liyanage,전유진(You-Jin Jeon) 한국해양바이오학회 2020 한국해양바이오학회지 Vol.12 No.2
A variety of shellfish species sold for human consumption are available for purchase in the domestic fish market. The microalgae families inhabit the ocean, where planktons supply the main nutritional resource for the growth of shellfish. Some phytoplanktons produce toxic compounds that are accumulated in shellfish and ultimately cause toxicity in humans. This article reports the cytotoxicity of commercially available shellfish species. Accordingly, hot water extract (HWE) and an aqueous fraction of 50% methanol extract (MEE-AF) showed no significant cytotoxicity on the two cell lines (i.e., HL-60 and Vero cell lines), but 50% methanol extract (MEE) in 3, 6 samples showed 50% cytotoxic effects on HL-60 cells, and 1, 4 samples showed 40%, 20% cytotoxic effects on Vero cells, respectively. In addition, their consequential dichloromethane fractions (MEE-DF) exhibited significant toxicities at the highest concentration (1,000 μg/ml) on HL-60 and Vero cells. Since the shellfish samples showed cytotoxicity in the dichloromethane fraction, it is possible that the dichloromethane fraction contains marine toxins. Further research will be needed to identify the toxic components from each sample.
라카디브 해역 몰디브 자생 해조류 Padina boryana 추출물의 항산화 효능 평가
김현수 ( Hyun-soo Kim ),( Lei Wang ),( Thilina U. Jayawardena ),이정민 ( Jeong Min Lee ),임미진 ( Mi-jin Yim ),고석천 ( Seok-chun Ko ),이효근 ( Hyo-geun Lee ),제준건 ( Jun-geon Je ),전유진 ( You-jin Jeon ),이대성 ( Dae-sung 한국수산과학회(구 한국수산학회) 2021 한국수산과학회지 Vol.54 No.2
Global warming has affected the distribution of organisms for decades and has displayed rapid ascent recently. Research into the effects on tropical organisms are vital. Padina boryana is a resourceful marine microalgae in the Maldives Sea in the Laccadive region. A 70% ethanol extraction (PBE) of this seaweed was used to investigate its antioxidant potential. Both in vitro and in vivo models were implemented. PBE exhibited protective potential against H2O2 induced apoptosis. ROS levels were suppressed due to PBE. PBE expressed a cytoprotective nature. In vivo experiments involving the zebra fish model conformed its validity. The antioxidant efficacy of PBE was dose dependent. Study outcomes suggest PBE has potential as a novel and valuable marine resource to aid the functional food and cosmeceutical industries.