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Poly(Lactic-co-Glycolic acid)(PLGA) 지지체 표면에 플라즈마 처리가 연골세포의 부착 및 활성에 미치는 영향
정지락,심형곤,박재구,주영민,·한동근,이종원,안상태 대한성형외과학회 2003 Archives of Plastic Surgery Vol.30 No.4
The advantages of wielding synthetic polymer are that the pore size, physical strength and chemical composition can be easily controlled. However, the synthetic polymer has hydrophobic character and low affinity to cells and other bio molecules. This study was performed to investigate the effect of plasma glow's discharge on the surface modification of poly(lactic-co-glycolic acid)(PLGA) sponge on the adhesion and bio-activity of chondrocytes in vitro culture.PLGA sponges(lactic acid: glycolic acid = 85 : 15, pore size=200-300μm, dimension=15×2mm) were prepared. The experimental group(n=8) was treated with a radiofrequency plasma glow-discharge(acrylic acid and oxygen: 50 W, 0.2 torr for 30 seconds) but the control group (n=8) was treated with nothing. 1×106ml/20 microliter of P3 chondrocytes from rabbit ears were used for seeding. Eight hours after cell seeding, the total DNA amount of chondrocytes attached to the PLGA and the changes of actin were evaluated under a confocal microscope. Type I and II collagen expression were detected by RT-PCR three weeks after seeding for an evaluation of phenotypic maintenance. The total DNA amount of attached chondrocytes was remarkably increased in the experimental group(p<0.05). After scrutinizing with a confocal microscope, the actin of cells was more spread out and finely arranged in the experimental group than in the control group. Both types of collagen expression were significantly increased in their assigned groups.Plasma treatment of the PLGA sponge could increase the adhesion property of chondrocytes, and provide suitable environment for maintaining the phenotype of chondrocytes.
정지열,허지웅,정예지,구경녀,최권락,소병재,윤순식,Jung, Ji-Youl,Her, Ji-Woong,Jeong, Yea-Ji,Ku, Kyung-Nyer,Choi, Kwon-Rac,So, Byung-Jae,Yoon, Soon-Seek 대한수의학회 2015 大韓獸醫學會誌 Vol.55 No.2
An abnormally enlarged right ovary and a mass in fat surrounding the right kidney were discovered in a dairy cow during routine postmortem examination at slaughter. The ovary was dark reddish and multinodular in shape. Numerous cystic structures were identified in the mass. Histopathologically, the ovary was completely replaced with large, uniform, polyhedral neoplastic cells containing vesicular nuclei and prominent nucleoli. The mitotic index was high. In the lymphatic vessels, tumor emboli were observed. Another mass in the fat surranding the right kidney had the same histological features as the ovarian mass. This animal was diagnosed with malignant dysgerminoma and metastasis to other peritoneal organs.
류문영,정지열,허지웅,이명헌,구경녀,최권락,윤순식,Rhyoo, Moon-Young,Jung, Ji-Youl,Her, Ji-Woong,Lee, Myoung-Heon,Ku, Kyung-Nyer,Choi, Kwon-Rac,Yoon, Soon-Seek 대한수의학회 2014 大韓獸醫學會誌 Vol.54 No.4
In this study, we describe a case of epitheliogenesis imperfecta (EI) observed in the fetus of Korean native cattle. The fetus had multifocal areas of skin defect, especially on the distal portions of the four limbs, and the affected areas were bright-red and glistening. Histopathologically, these areas were characterized by complete absence of squamous epithelium, infiltration of inflammatory cells into the dermis, atrophy of hair follicles, sebaceous and sweat glands. To the best of our knowledge, this is the first report of epitheliogenesis imperfecta in Korean native cattle.