흰쥐에서 당뇨 유도 후 하악골 골밀도 변화에 관한 연구

정석영(Seok-Young Jeong),신상훈(Sang-Hun Shin),김욱규(Uk-Kyu Kim),박봉수(Bong-Soo Park),정인교(In-Kyo Chung) 대한구강악안면외과학회 2002 대한구강악안면외과학회지 Vol.28 No.2

DM is a systemic disease with many complications. One of them, diabetic osteopenia is important sequelae and many authors reported reduced bone mass in diabetic rats. However, in mandible, study has been rare because of its anatomical limits. So the objective of this study was to investigate bony change in mandible of diabetic rats. Thirty-two adult rats were used in this study. Half of them were male and female respectively. In sixteen rats, streptozotocin was injected intraperitoneally to induce DM and the serum glucose concentration was checked to ensure the induction of DM prior to the time of sacrifice. At 1, 2, 3, 4, 6, 8, 12, 16weeks, control group and diabetic group rats were sacrificed respectively. And then bone mineral density of mandibles and femurs of the rats was measured using dual energy X-ray absorptiometry(DEXA). In addition serum osteocalcin and urine deoxypyridinoline were measured as markers of bone formation and resoption respectively. Mandibular and femoral bone density in streptozotocin induced rats was decreased with significance statistically after 4 weeks from injection. In mandible, comparing with femur, bone density was moderately decreased. The alveolar bone in mandible was more decreased bone density than the whole body in the mandible From these results, bone mineral density decreased in uncontrolled diabetic group with time, and especially alveolar bone was more destructive in the mandible. So authors think that consideration of reduced bone mineral density is necessary in dental procedure.

골다공증 유도 흰쥐에서 칼슘과 비타민 D 섭취가 티타늄 임플랜트 주위의 골 형성에 미치는 영향

이재열(Jae-Yeol Lee),정석영(Seok-Young Jeong),신상훈(Sang-Hun Shin),김규천(Gyoo-Cheon Kim),김용덕(Yong-Deok Kim),정인교(In-Kyo Chung),김욱규(Uk-Kyu Kim) 대한구강악안면외과학회 2008 대한구강악안면외과학회지 Vol.34 No.3

Purpose: The purpose of this study was to observe the effect of calcium and vitamin D to the titanium implant osseointegration in the osteoporosisinduced animal model. Material and method: Thirty-two rats, 10 weeks of age, were divided into two groups: experimental group was ingested additional calcium and vitamin D , and a control group was not. Titanium screw implant(diameter, 2.0 mm; length, 3.5 mm; pitch-height 0.4 mm) were placed into tibia of 32 rats, 16 in the control group and 16 in the experimental group. The rats were sacrificed at 1, 2, 4 and 8 weeks after implantation for histopathologic examination, histomorphometric analysis and immunohistochemistry with fibronectin and collagen type I antibody. Result: In histopathological findings, newly formed bone was seen at 2 weeks and became lamellar bone at 4 weeks, and mature trabecullar bone was seen at 8 weeks in experimental group. In control group, thickness of regenerated bone increased till 4 weeks gradually and trabecullar bone was seen at 8 weeks. In histomorphometric analysis, marrow bone density increased significantly in experimental group compared to control group. Fibronectin immunoreactivity was strong at 2 weeks in experimental group and reduced after 4 weeks gradually. But it was maintained continuously from 2 to 8 weeks in control group. Collagen type I immunoreactivity was very strong from 2 to 4 week in experimental group. And the amount of Collagen type I expression was more abundant in experimental group. Conclusion: The results of this study suggest that calcium and vitamin D supplementation promote bone healing around titanium implants in osteoporosis induced animals.

악하선에서의 Na⁺, k⁺- activated adenosine triphosphatase의 특성에 관한 실험적 연구

In kyo Jeong(鄭仁敎),Eui Wung Lee(李義雄) 대한구강악안면외과학회 1983 대한구강악안면외과학회지 Vol.9 No.1

The Na-K-ATPase was isolated from cat submaxillary gland by a deoxyclolate-treated procedure. In order to determine the characteristics of Na-K-ATPase of submaxillary gland, effects of incubation time, medium composition, temperature and various drugs were investigated. The results were summarized as fellows: 1. The specific activities of Na-K-ATPase and Mg-ATPase were 0.58±0.04 and 0.42±0.03μmol Pi/㎎ protein/min, respectively, in twice deoxycholate-treated microsome. 2. The initial velocity of the Na-K-ATPase reaction was directly proportional to the incubation time and to the enzyme concentration in the medium. 3. The rate of the reaction was dependent on the concentrations of ATP, Mg, Na, K and H ions in the medium, showing maximal activity at 2mM ATP, 3mM Mg, 100mM Na and 10mM K. 4. The maximal activity of Na-K-ATPase was observed in pH 7.5 but Mg-ATPase activity increased by increasing of medium pH. 5. The Na-K-ATPase activity was inhibited by ouabain and ethacrynic acid, but not affected by NaN₃, acetylcholine and mitomycin-C. 6. The Arrhenius plot showed biphasic pattern and the higher activation energy at lower range(10-25℃) than higher temperature range(25-37℃). These results indicate that the Na-K-ATPase of submaxillary gland of cat is similar to that of the other tissue in its physiological and biochemical properties.

금앵자 에탄올 추출물에 의한 3T3-L1 지방세포의 분화억제 효과와 그 메커니즘 규명

정현영 ( Hyun Young Jeong ),정인교 ( In Kyo Jeong ),남소연 ( So Yeon Nam ),윤희정 ( Hee Jung Yun ),김병우 ( Byung Woo Kim ),권현주 ( Hyun Ju Kwon ) 한국미생물생명공학회(구 한국산업미생물학회) 2016 한국미생물·생명공학회지 Vol.44 No.1

비만은 체내 지방이 과도하게 축적되어 일어나는 현상으로, 당뇨, 고혈압, 심혈관 질환 및 암과 같은 질병의 원인이 된다. 본 연구는 RLE에 의해 지방전구세포에서 지방세포로 분화 시, 세포 내 축적되는 Triglyceride 저해 및 발현되는 전사인자들의 발현양상에 미치는 영향에 대하여 조사하였다. 그 결과, RLE는 Oil Red O 염색에서 세포 내 triglyceride의 축적을 농도 의존적으로 억제하였다. 또한 CCAAT/enhancer binding protein(C/EBP) α, β와 peroxisome proliferator activated receptor γ(PPARγ)과 같은 지방세포 분화 관련 전사인자들의 발현을 억제하였다. RLE는 clonal expansion 단계의 지방세포를 G1기에서 세포 주기를 정지시켜 세포의 증식을 억제하였으며, RLE 처리에 의해 p21의 증가, Cyclin E, Cdk2, Phospho-Rb의 발현 저해 등 G1 arrest 관련 단백질의 발현 변화가 유도되었다. 따라서, RLE는 분화 관련 전사인자들의 발현을 조절하고 지방세포 분화 초기에 G1기의 세포 주기 정지를 억제함으로써 지방전구세포에서 지방세포로의 분화를 억제한다고 사료된다. Obesity is caused by excess accumulation of body fat and contributes to various pathological disorders such as diabetes, hypertension, cardiovascular disease, and cancer. In this study, we investigated the effect of a 30% ethanol extract of Fructus Rosae laevigata (RLE) on adipogenesis in 3T3-L1 adipocytes, measured by triglyceride accumulation and expression of adipogenesis-related transcription factors during differentiation of pre-adipocytes into adipocytes. RLE decreased the intracellular triglyceride contents (assessed by Oil Red-O staining) in a dose-dependent manner. It also downregulated the expression of adipogenic transcription factors and inhibited cell proliferation during the mitotic clonal expansion phase of adipocyte differentiation by inducing G1 phase arrest. We investigated the alterations in the levels of G1 phase arrest-related proteins. The expression of p21 protein significantly increased, while the levels of Cyclin E, Cdk2, and phospho-Rb decreased in a dose-dependent manner in 3T3-L1 cells treated with RLE. These results suggest that RLE inhibits the differentiation of 3T3-L1 adipocytes by suppressing the expression of adipogenic transcription factors and inducing G1 phase arrest in the early stages of adipocyte differentiation.

팔각회향 dichloromethane 분획물에 의한 지방세포 분화 억제 효과

정현영(Hyun Young Jeong),정인교(In Kyo Jeong),김남주(Nam Ju Kim),윤희정(Hee Jung Yun),박정하(Jung Ha Park),김병우(Byung Woo Kim),권현주(Hyun Ju Kwon) 한국생명과학회 2019 생명과학회지 Vol.29 No.4

비만은 에너지의 불균형으로 인하여 체내 지방조직에 지방이 축적되는 대사성질환으로 심혈관계 질환, 고혈압, 2형 당뇨, 고지혈증 및 각종 암의 발생 빈도를 증가시키는 요인이다. 지방의 축적은 지방전구세포가 지방세포로 분화하는 과정을 의미하는 adipogenesis라는 과정을 거쳐서 일어난다. 지방세포로의 분화는 다양한 호르몬과 전사인자들의 상호작용에 의해서 일어난다. 본 연구에서는 팔각회향이 항비만 소재로 활용 가능한지 확인하기 위해, 팔각회향 물 추출물을 분획하여 지방축적 억제 활성이 좋은 dichloromethane층을 선정하였다. 3T3-L1 지방전구세포가 성숙한 지방세포로 분화할 때 팔각회향 dichloromethane 층이 어떠한 기전으로 분화를 조절하는지 확인한 결과, 지방세포 분화에서 중요한 전사인자인 C/EBP family, PPARγ의 발현이 억제되었고, 지방세포 최종 분화마커로 알려져 있는 FAS 및 LPL의 발현 또한 감소되었다. 또한 G1기에서의 세포주기 정지를 통해 지방세포 분화단계에서 필수적인 mitotic clonal expansion 단계를 억제한다는 결과를 얻었다. 이러한 연구 결과는 팔각회향이 항비만 효과를 가지는 천연물 소재로의 활용가능성을 보여주는 기초 자료가 될 것으로 사료된다. Fat accumulation in adipocytes occurs through the process of adipogenesis in which preadipocytes differentiate into adipocytes. Obesity is a metabolic disorder caused by excessive accumulation of fat in the body, which increases the incidence of cardiovascular diseases, hypertension, type 2 diabetes, hyperlipidemia, and various cancers. Recently, inhibition of adipocyte differentiation was shown to be a potential antiobesity strategy. In this study, the inhibitory effect of dichloromethane fractions from Illicium verum Hooker fil. water extract on the differentiation of 3T3-L1 preadipocytes to adipocytes was investigated. Dichloromethane fractions from I. verum Hooker fil. significantly inhibited adipocyte differentiation when applied during the adipocyte differentiation process, as assessed by measuring fat accumulation using Oil-red O staining. In addition, dichloromethane fractions from I. verum Hooker fil. reduced important adipogenic transcription factors, such as CCAAT/enhancer binding protein α (C/EBPα), C/EBPβ, and peroxisome proliferator activated receptor γ (PPARγ). The expression of FAS and LPL, which are terminal differentiation markers of mature adipocytes, was also reduced in the 3T3-L1 adipocytes treated with dichloromethane fractions from I. verum Hooker fil. In addition, the treatment significantly inhibited mitotic clonal expansion, which is essential for adipocyte differentiation, by arresting the G1 phase of the cell cycle. Taken together, these results suggest that dichloromethane fractions from I. verum Hooker fil. may be a natural material with antiobesity effects.

Hyper-Rectangles를 이용한 단일 분류기 설계

정인교(In Kyo Jeong),최진영(Jin Young Choi) 대한산업공학회 2015 대한산업공학회지 Vol.41 No.5

선박용 LED 등기구의 알루미늄 합금 방열판의 방열성능 향상을 위한 플라즈마 전해 산화의 공정변수 선정에 관한 연구

이정형,정인교,한민수,Lee, Jung-Hyung,Jeong, In-Kyo,Han, Min-Su 한국표면공학회 2018 한국표면공학회지 Vol.51 No.6

The possibility of an improvement in heat dissipation performance of aluminum alloy heat sink for shipboard LED luminaries through plasma electrolytic oxidation (PEO) was investigated. Four different PEO coatings were produced on aluminum alloy 5052 in silicate based alkaline solution by varying current density ($50{\sim}200mA/cm^2$). On voltage-time response curves, three stages were clearly distinguished at all current densities, namely an initial linear increase, slowdown of increase rate, and steady state(constant voltage). It was found that the increase in current density caused the breakdown voltage to increase. Two different surface morphologies - coralline porous structure and pancake structure - were confirmed by SEM examination. The coralline porous structure was predominant in the coatings produced at lower current densities (50 and $100mA/cm^2$) while under high current densities(150 and $200mA/cm^2$) the pancake structure became dominant. The coating thickness was measured and found to be in a range between about $13{\mu}m$ and $44{\mu}m$, showing increasing thickness with increasing current density. As a result, $100mA/cm^2$ was proposed as an effective process parameter to improve the heat dissipation performance of aluminum alloy heat sink, which could lower the LED operating temperature by about 30%.

악골에 발생한 다발성 낭종의 치험례

김종렬(Jong Ryoul Kim),양동규(Dong Kyu Yang),정인교(In Kyo Jeong),김욱규(Uk Kyu Kim),조윤일(Yun Il Cho),최창수(Tchang Soo Tchoi) 대한악안면성형재건외과학회 1988 Maxillofacial Plastic Reconstructive Surgery Vol.10 No.1

This is case presentation in three patients who have shown multiple cysts in jaws. We obtained the following results through the postoperative periodic check. 1. The multiple cysts were mainly appeared on the unerupted teeth, especially the 3rd molars. 2. Among the case reports, one case was multiple dentigerous cysts associated with maxillary left and right 3rd molar areas and mandibular left and right 3rd molar areas, the other case presented the primordial cyst on the right maxillary and left mandibular 3rd molar areas, the another case was multiple dentigerous cysts associated with impacted teeth on mandibular body areas. 3. The familiar tendency, peculiar feature of multiple cyst, was obtained only from one case report. 4. Though primary closure on the large-sized cysts, total case reports showed good healings.

Cone Beam Computed Tomography를 이용한 상악 임플란트 식립 전후의 골밀도 변화에 관한 연구

최정훈,이주민,김용덕,신상훈,정인교,Choi, Jeong-Hun,Lee, Ju-Min,Kim, Yong-Deok,Shin, Sang-Hun,Chung, In-Kyo 대한악안면성형재건외과학회 2010 Maxillofacial Plastic Reconstructive Surgery Vol.32 No.3

Purpose: This study examined the significance of increased bone density according to time after implantation on maxilla using demographic data with CBCT and compared the bone density between before vs. after implantation using the Hounsfield index. Materials and Methods: Twenty-five implant site on maxilla were selected. Cone-beam computerized tomography (CBCT) scans were used for the analysis. The implant sites were evaluated digitally using the Hounsfield scale with EzImplant TM and the results were compared over time. Statistical data over time was carried out to determine the correlation between the recorded Hounsfield unit (HU) over time and gender difference using repeated ANOVA. Results: The bone density of implantation site over time showed an increase in the HU mean values. Immediately after implantation, bone density was significantly increased than bone density before implantation. Until 6 month follow-up, bone density showed stable increasement. There is no significant difference on gender. Conclusions: Using CBCT, bone density increased over time after implantation on maxilla. Bone density measurements using CBCT might provide an objective assessment of the bone quality as well as the correlation between bone density and stability of implant.

동아시아 FTA Hub 전략의 경제적 효과와 타당성 검토

정재화,정인교 韓國東北亞經濟學會 2009 동북아경제연구 Vol.21 No.1

East Asian countries have continued to pursue an active FTA policy since the 1997 Asian financial crisis. In the region, ASEAN+1 type of FTAs are already in effect or have been concluded between the ASEAN and the three Northeast Asian countries (Korea, Japan, China) along with the deeper integration of the AFTA which combines the ten ASEAN member countries. At the same time, active discussions have been conducted on a possible FTA that covers the entire East Asian region, an East Asia FTA (EAFTA). If this EAFTA is concluded, it is expected to surpass the size of NAFTA and to have even greater ripple effects. According to the CGE analysis on the economic effects of an East Asia FTA (EAFTA), the FTA is expected to be beneficial to the countries in the region. A regional FTA among countries in Northeast Asia or East Asia will have greater rewards than bilateral FTAs between two countries. In particular, Korea is deemed to be the greatest beneficiary of an EAFTA. And if China and Japan do not conclude an FTA and Korea concludes FTAs with ASEAN China and Japan and becomes an East Asian FTA hub, the economic effects are expected to be even greater. Notwithstanding the economic benefits that come from being an FTA hub, the merits of an East Asia FTA covering the entire region will be even greater. First of all, it will be possible to lower the rising transaction costs that ensue from the intertwined regional FTA network Also, higher economic efficiency is expected in the mid-term and long-term from the harmonization of the different systems. Therefore, the straightforward strategy for Korea would be to pursue an East Asia FTA and strategically promote FTAs with Japan and China to get a strategic advantage in the future East Asia FTA negotiation process. In fact, under the current situation in which an FTA among the three major countries in Northeast Asia, Korea, China and Japan, is deterred by various obstacles, an East Asia FTA could be the favored alternative. This is because an FTA combining the entire region could alleviate the conflicting interests of individual industry sectors that may arise from a bilateral FTA. In addition, it would be possible to establish a more flexible level of trade liberalization in goods under an East Asia FTA.