Rifampicin과 Ofloxacin에 내성인 Bifidobacterium bifidum 균주의 개발

정영자,전명인,강창율,김병각,최응칠,Chung, Young-Ja,Jeon, Myoung-In,Kang, Chang-Youl,Kim, Byoung-Kak,Choi, Eung-Chil 대한약학회 1994 약학회지 Vol.38 No.6

Bifidobacterium bifidum, one strain of medical preparation being on the market for human intestinal disorders, was sensitive to rifampicin and fluoroquinolones. If this preparation is taken with rifampicin and fluoroquinolones, its therapeutic effect can't be expected. Serial passage of B. bifidum RFR61, which was obtained by MNNG mutation method, on agar with 2-fold minimal inhibitory concentration of ofloxacin produced B. bifidum OFR9 with minimal inhibitory contentrations of fluoroquinolones up to $4{\sim}256-fold$ higher than that for the original strain. B. bifidum OFR9 produced almost the same amount of organic acid as parental strain. This strain showed growth inhibitory activity against E. coli NM522, Shigella dysenteriae ATCC9752 and E. coli 078. No inactivations of rifampicin and ofloxacin by this resistant mutant strain were found.

Bifidobacterium bifidum에서 리팜피신에 대한 내성기전

정영자(Young Ja Chung),박성수(Seong Soo Park),백문창(Moon Chang Baek),김병각(Byong Kak Kim),최응칠(Eung Chil Choi) 대한약학회 1998 약학회지 Vol.42 No.2

Bifidobacterium bifidum OFR9 that exhibits acquired resistance to rifampicin and fluoroquinolones was selected by MNNG and multi-step mutation method. To investigate the resistance mechanism to rifampicin in the strain, RNA polymerase from B. bifidum parent strain and rifampicin-resistance OFR9 was partially purified and its sensitivity to rifampicin was assayed. The profile of RNA polymerase preparation of B. bifidum parent and B. bifidum OFR9 is similar to that of E. coli RNA polymerase that includes the basic subunits of beta`, beta, sigma, alpha but which are a little different in size when they are compared with E. coli RNA polymerase subunits. RNA polymerase isolated from the parent strain was inhibited by 1mcg/ml rifampicin but that from B. bifidum OFR9 was not affected by 100mcg/ml concentration of rifampicin. RNA polymerase activity of B. bifidum OFR9 was maintained over 90% through that rifampicin concentration. This result is consistent with MIC values of in vitro test. It can be concluded that the mechanism of rifampicin resistance in B. bifidum OFR9 is due to an alteration of RNA polymerase.

마크로라이드-린코사마이드-스트렙토그라민 B(MLS)계 항생물질에 대한 유도 내성

권애란(Ae Ran Kwon),최성숙(Sung Sook Choi),김숙경(Sook Kyung Kim),정영자(Young Ja Chung),최응칠(Eung Chil Choi),김병각(Byoung Kak Kim) 대한약학회 1994 약학회지 Vol.38 No.3

Forty nine clinical isolates of S. aureus showing resistance to erythromycin(EM) were selected from 83 strains isolated recently in Korea. Fourteen strains of S. aureus showing inducible resistance to MLS antibiotics were selected by disc agar diffusion method. Colony hydridization was executed using two MLS inducible resistance genes, ermA and ermC, identified previously from S. aureus as probes. S. aureus 375 and S. aureus 507 whose genes were not homologous to those probes were finally selected. It was confirmed that the resistance genes of S. aureus 375 and S. aureus 507 had no homology with those probes in southern hybridization test using ermA, ermC and ermAM as probes. It was determined that S. aureus 375 had a plasmid whose size was about 35 kb. To know if the plasmid may have the genes related to inducible resistance to MLS antibiotics, it was attempted to transform Bacillus subtillis BR151 and S. aureus RN4220 with the plasmid isolated from S. aureus 375. It was shown that the gene related to inducible resistance to MLS antibiotics did not exist in this plasmid. These results indicate that two clinical isolates of S. aureus showing inducible resistance to MLS antibiotics have novel genes that have no homology with MLS resistance genes identified so far. It is assumed that these genes may exist in chromosomal DNA.

리팜피신과 오플로삭신에 내성인 Enterococcus faecalis 균주의 개발

이수화(Soo Hwa Lee),김숙경(Sook Kyung Kim),정영자(Young Ja Chung),심미자(Mi Ja Shim),김병각(Byong Kak Kim),최응칠(Eung Chil Choi) 대한약학회 1996 약학회지 Vol.40 No.3

The preparation of Enterococcus faecalis RSI is used as a therapeutics for human intestinal disorders. However, the microbe in this preparation is usually very sensitive to rifampicin and fluoroquinolones. If this preparation is taken with rifampicin or fluoroquinolones, its therapeutic effect can not be expected. E. faecalis RFR11, containing resistance to rifampicin was obtained by MNNG mutation method. Serial passage of E. faecalis RFR11 produced E. feacalis OFR16 on agar with 2-fold minimal inhibitory concentration of ofloxacin produced. E. feacalis OFR16 was resistant to fluoroquinolones up to 8-256 fold higher than that for the original strain. E. faecalis OFR16 also exhibited identical characteristics with the parent strain when they were tested for lactic acid formation and growth inhibition of E. coli MB4-5737 and Shigella sonnei MB4-10411. From in vitro test, it was identified that rifampicin and ofloxacin is not inactivated by certain factors of E. faecalis OFR16. Conclusively. E. faecalis OFR16, rifampicin and fluoroquinolones resistant mutant, is an efficient strain that has insensitivity against rifampicin and fluoroquinolones and original biochemical characteristics of the parent strain.

圓佛敎가 한국의 女性開化에 미친 影響

정영자 圓光大學校 圓佛敎學硏究會 1983 圓佛敎學硏究 Vol.13 No.-

Intracellular Ca^(2+) Mediates Lipoxygenase-induced Proliferation of U-373 MG Human Astrocytoma Cells

Kim, Jung-Ae,Chung, Young-Ja,Lee, Yong Soo 영남대학교 약품개발연구소 1999 영남대학교 약품개발연구소 연구업적집 Vol.9 No.-

The role of intracellular Ca^(2+) in the regulation of tumor cell proliferation by products of arachidonic acid (AA) metabolism was investigated using U-373 MG human astrocytoma cells. Treatment with nordihydroguaiaretic acid (NDGA), a lipoxygenase (LOX) inhibitor, or caffeic acid (CA), a specific 5-LOX inhibitor, suppressed proliferation of the tumor cells in a dose-dependent manner. However, indomethacin (Indo), a cyclooxygenase (COX) inhibitor, did not significantly alter proliferation of the tumor cells. At anti-proliferative concentrations, NDGA and CA significantly inhibited intracellular Ca^(2+) release induced by carbachol, a known intracellular Ca^(2+) agonist in the tumor cells. Exogenous administration of leukotriene B­(4) (LTB­(4)), an AA metabolite of LOX pathway, enhanced proliferation of the tumor cells in a concentration-dependent fashion. In addition, LTB­(4) induced intracellular Ca^(2+) release. Intracellular Ca^(2+) inhibitors, such as an intracellular Ca^(2+) chelator (BAPTA) and intracellular Ca^(2+) -release inhibitors (dantrolene and TMB-8), significantly blocked the LTB­(4)-induced enhancement of cell proliferation and intracellular Ca^(2+) release. These results suggest that LOX activity may be critical for cell proliferation of the human astrocytoma cells and that intracellular Ca^(2+) may play a major role in the mechanism of action of LOX.

The Analysis of Volatile Organic Compounds in Water by Using the Purge-and-Trap and the Gas Chromatography/Mass Selective Detector with Modified Indirect Coupling

Park, Seong-Soo,Chung, Young-Ja,Min, Kyung-Chan,Park, Taek-Kyu 한국식품영양학회 1999 韓國食品營養學會誌 Vol.12 No.2

40종류의 휘발성 유기화합물들을 물시료로부터 분리하기 위하여 purge & trap과 GC/MSD를 사용하였다. Purge & trap에 사용되는 파라미터들을 조절하여 퍼지 효율성을 증가시킬 수 있었다. Purge & trap의 최적조건은 다음과 같다 : 퍼지 시간 11분, 건조퍼지 시간 5분, 시료온도 60℃, 일정한 퍼지유속(40ml/min), 일정한 탈착유속(20ml/min), 탈착온도(225℃) 그리고 탈착시간(1분). 이러한 최적조건에서 각각의 화합물의 퍼지 효율성은 70% 이상이었다. A Purge & Trap Concentrator was used to analyze various volatile organic compounds(VOCs) in water. The object of this study was to observe the purge efficiency of 40 VOCs in water according to the change of parameters (purge time, drypurge time, sample temperature) and to determine the optimum condition for VOCs using the Purge & Trap concentrator interfaced with a narrow capillary connected to a gas chromatography/mass spectrometry. The optimum condition of purge and trap is as follows : purge time at 11min, drypurge time at 5min, sample temperature at 60℃ at constant purge flow (40ml/min), constant desorption flow(20ml/min(, desorpton temperature(225℃) and desorption time (1min). At this analytical condition, the detection limits of VOCs was in the range of 0.1∼0.5㎍/ml and the purge efficiency of each compound was over 70%.