한국산 고등 균류의 성분 연구 9 제48보 ) 노란다발버섯의 독 성분 네마톡신

김병각,심미자 ( Byong Kak Kim,Mi Ja Shim ) 한국균학회 1984 韓國菌學會誌 Vol.12 No.3

한국산 맥각의 알칼로이드 성분 연구 ( 1 )

김병각,황성현,최응칠,권창호 ( Byong Kak Kim,Sung Hyun Whang,Eung Chil Choi,Chang Ho Kwon ) 생화학분자생물학회 1974 BMB Reports Vol.7 No.1

Korean ergot sclerotia which were collected from Agropyron semicostatum Nees, Miscanthus sinensis Anders. var. purpurascens Rend., M. sacchariflorus Benth. et Hook., M. sinensis Anders., Eulalia speciosa O. Kuntz, Themeda triandra Forsk. var. japonica Makino, and Phalaris arundinacea L. were investigated for their alkaloid constituents. Quantitative determinations of alkaloids were carried out using a modified procedure of van Urk and Michelon-Kelleher methods. The results showed that the two ergots from Agropyron semicostatum and Phalaris arundinacea contained total alkaloids up to 0. 137 per cent. The alkaloids of these two ergots are mostly water-insoluble alkaloids. The ergot parasitizing Reed canary grass was found to contain both water-soluble and water-insoluble alkaloids. The TLC and PPC tests for the ergot alkaloids from Agropyron semicostatum and Phalaris arundinacea revealed that they contain at least two alkaloids respectively and that the ergot from Themeda triandra Forsk var. japonica has one. The lipid contents of the seven ergots ranged from 13 to 29 per cent.

맥각균의 배양에 관한 연구 ( 3 ) 발효조에서의 Lysergic Acid 알칼로이드의 생성

김병각,옥선,최응칠 ( Byong Kak Kim,Sun Auck,Eung Chil Choi ) 생화학분자생물학회 1973 BMB Reports Vol.6 No.3

Ergot fungus was culitvated in stirred fermentors in various modified conditions to investigate their influence on the production of lysergic acid alkaloids. Claviceps paspali Stevens and Hall Isolate No. 31 was cultured in mannitol-succinate medium of Schwarting, et al., and such modifications as additions of Tween 80, oxygen gas, dimethylsulfoxide, arsenate and the utilization of sorbitol-fumaric acid medium and propeller-type impellers were respectively examined. Quantitative determination of the total alkaloids was carried out using Michelon-Kelleher method. The results showed that additions of oxygen gas, dimethylsulfoxide, arsenate, and the utilization of sorbitol-fumaric acid medium increased the production of the alkaloids. However, the addition of Tween 80 caused extensive foaming and resulted in decrease of the alkaloid accumulation.

한국산 맥각의 알칼로이드 성분 연구(I)

김병각,황성현,최응칠,권창호,Kim, Byong-Kak,Whang, Sung-Hyun,Choi, Eung-Chil,Kwon, Chang-Ho 생화학분자생물학회 1974 한국생화학회지 Vol.7 No.1

한국에서 자라는 화본과(Graminae) 식물인 Agropyron semicostatum Nees, Miscanthus sinensis Anders. var. purpurascens Rend., M. sacchariflorus Benth. et Hook., M. sinensis Anders., Eulalia speciosa O. Kuntz, Themeda triandra Forsk. var. japonica Makino, 및 Phalaris arundinacea L.의 7종에 기생하는 맥각을 채집하여 그 알칼로이드 성분을 분석하였다. 알칼로이드의 정량으로써 van Urk 법과 Michelon-Kelleher 법의 장점을 취하여 개량한 방법을 이용하여 시행하였던 바 Agropyron semicostatum과 Phalaris arundinacea의 각 맥각이 0.137%까지의 알칼로이드 총량을 함유하였다. 이 두가지 맥각의 알칼로이드의 종류는 주로 비수용성 알칼로이드였다. 후자의 식물에 기생한 맥각은 수용성 및 비수용성 알칼로이드를 모두 함유하고 있었다. 이 두가지 액각의 알칼로이드를 TLC 및 PPC 법으로 분석하여 본바 각각 적어도 2종의 알칼로이드를 함유하고 Themeda triandra Forsk. var. japonica에 기생한 맥각은 1종을 함유하고 있음을 알았다. 7종의 맥각의 지질 함량은 13~29% 였다. Korean ergot sclerotia which were collected from Agropyron semicostatum Nees, Miscanthus sinensis Anders. var. purpurascens Rend. M. sacchariflorus Benth. et Hook., M sinensis Anders., Eulalia speciosa O. Kuntz, Themeda triandra Forsk. var. japonica Makino, and Phalaris arundinacea L. were investigated for their alkaloid constituents. Quantitative determinations of alkaloids were carried out using a modified procedure of van Urk and Michelon-Kelleher methods. The results showed that the two ergots from Agropyron semicostatum and Phalaris arundinacea contained total alkaloids up to 0.137 per cent. The alkaloids of these two ergots are mostly water-insoluble alkaloids. The ergot parasitizing Reed canary grass was found to contain both water-soluble and water-insoluble alkaloids. The TLC and PPC tests for the ergot alkaloids from Agropyron semicostatum and Phalaris arundinacea revealed that they contain at least two alkaloids respectively and that the ergot from Themeda triandra Forsk var. japonica has one. The lipid contents of the seven ergots ranged from 13 to 29 per cent.

맥각균의 배양에 관한 연구(III) - 발효조에서의 Lysergic Acid 알칼로이드의 생성

김병각,옥선,최응칠,Kim, Byong-Kak,Auck, Sun,Choi, Eung-Chil 생화학분자생물학회 1973 한국생화학회지 Vol.6 No.3

맥각균을 발효조에서 배양할때 라이서직산 알칼로이드의 생성에 미치는 배양조건 및 첨가물질의 영향을 검토하기 위하여 Claviceps paspali Stevens and Hall 31호 균주를 Schwarting 배양액에서 배양하였으며 Tween 80, 분자상의 산소, 디메칠설폭사이도, 비산 나트륨을 각각 첨가했을 경우 및 솔비톨-푸마르산 배양액과 프로펠라형 교반기를 사용했을 경우의 영향을 실험하였다. 알칼로이드 총량의 정량은 Michelon-Kelleher 법을 이용하였다. 실험결과에 의하면 산소, 디메칠성폭사이드, 비산염의 각각 첨가 및 솔비톨-프마르산 배양액의 사용이 알칼로이드 생성을 증가하였다. 그러나 Tween 80의 첨가는 과잉의 포말을 발생시켜 배양액의 손실을 초래하였으며 결국 알칼로이드의 축적이 감소되었다. Ergot fungus was culitvated in stirred fermentors in various modified conditions to investigate their influence on the production of lysergic acid alkaloids. Claviceps paspali Stevens and Hall Isolate No.31 was cultured in mannitol-succinate medium of Schwarting, et al., and such modifications as additions of Tween 80, oxygen gas, dimethylsulfoxide, arsenate and the utilization of sorbitol-fumaric acid medium and propeller-type impellers were respectively examined. Quantitative determination of the total alkaloids was carried out using Michelon-Kelleher method. The results showed that additions of oxygen gas, dimethylsulfoxide, arsenate, and the utilization of sorbitol-fumaric acid medium increased the production of the alkaloids. However, the addition of Tween 80 caused extensive foaming and resulted in decrease of the alkaloid accumulation.

한국산 고등 균류의 성분 연구(VI) Amanita spissacea 및 Amanita vaginata의 아미노산

김병각,이영수,최응칠,심미자,이영남,Kim, Byong-Kak,Lee, Young-Soo,Choi, Eung-Chil,Shim, Mi-Ja,Lee, Young-Nam 생화학분자생물학회 1977 한국생화학회지 Vol.10 No.1

저자들은 Amanitaceae에 속하는 버섯의 일종인 Amanita spissacea와 Amanita vaginata의 형태학적 특징 및 이들 버섯중에 함유되어 있는 아미노산 분석을 시행하였다. gas chromatography를 이용하여 14종의 유리 아미노산을 확인하였고 8종의 유리 필수 아미노산을 정량하였다. 그리고 이미노산 자동분석기를 이용하여 이들 버섯의 산 가수분해물에 대해 총 아미노산 분석을 시행하였다. 그 결과 Amanita spissacea와 Amanita vaginata에서 14종의 유리 아미노산을 확인하였고 또한 8종의 유리 필수 아미노산중 둘 다 threonine이 가장 많아서 각각 7.3mg/g, 3.2mg/g이었고 총 아미노산 분석 결과 16종의 아마노산을 확인하였고 총 함량은 각각 138mg/g 및 166mg/g이었다. Amanita spissacea which is indiginous to Korea and Japan and Amanita vaginata which is an edible mushroom, grow wildly in Korea. However, their constituents have never been studied. These higher fungi were collected in the Gyeong Gi Province, Korea, and analyzed for their free and total amino acids, using gas chromatography and amino acid auto-analyzer. Fourteen free amino acids (alanine, glycine, valine, leucine, isoleucine, threonine, aspartic acid, methionine, phenylalanine, histidine, tyrosine, lysine, arginine, and tryptophan) were identified in both species. The contents of eight free essential amino acids in A. spissacea and a. vaginata were : threonine 7.26 and 3.15 mg/g, lysine 2.85 and 2.5 mg /g, methionine 2.48 and 2.70 mg/g, phenylalanine 1.35 and 0.98 mg/g, isoleucine and leucine combined 1.65 and 1.05 mg/g, valine 1.24 and 0.68 mg/g, and tryptophan 0.90 and 0.93 mg/g, respectively. The total contents of the respective amino acids in A. spissacea and A. vaginata were: lysine 5.18 and 12.28 rngj'g, histidine 3.00 and 5.12 mg/g, arginine 7.76 and 9.80mg/g, threonine 8.88 and 8.00 mg/g, glutamic acid 20.10 and 34.94 mg/g, alanine 10.22 and 11.62 mg/g, valine 9.64 and 8.94 mg /g, glycine 8.32 and 8.30 mg/g, methionine 1.90 and 1.63 mg/g, isoleucine 6.66 and 7.88 mg/g, leucine 12.74 and 14.00 mg/g, phenylalanine 7.00 and 7.82 mg/g, cysteine 0.82 and 1.26 mg/g, aspartic acid 16.94 and 15.28 mg/g, serine 9.84 and 10.34 mg/g and threonine 3.62 and 3.90 mg/g, respectively.

Claviceps paspali 의 액내배양에서의 라이써직산 알카로이드 생성에 대한 비산의 촉진작용

김병각,William J . Kelleher Arthur E . Schwarting ( Byong Kak Kim ) 생화학분자생물학회 1972 BMB Reports Vol.5 No.1

Analysis of the culture filtrates of lysergic acid alkaloid-producing fermentations at intervals during growth of the cultures showed that phosphate is most rapidly depleted from the medium in fermentations that produce the highest levels of alkaloid. In view of the known interference with phosphate metabolism by arsenate, sodium arsenate was added to the culture medium with the intent of creating, in effect, a phosphate deficiency. The addition of arsenate at levels between one-fiftieth and one-twentieth the molar concentration of phosphate resulted in increases in alkaloid production up to 100 per cent. Experiments with ten-times the usual concentration of phosphate showed that the ratio of arsenate to phosphate rather than the absolute concentration of arsenate was most significant in obtaining this effect. Increasing the concentration of phosphate alone (up to twenty times the usual concentration) resulted in substantial increases in alkaloid production. The effect of arsenate on both the rate and the extent of phosphate uptake was determined. Among several inhibitors of electron transport and/or oxidative phosphorylation, only the uncoupler of oxidative phosphorylation (dinitrophenol) was effective in increasing alkaloid production.

한국산 고등 균류의 성분 연구 ( Ⅵ ) Amanita Spissaceea 및 Amanita Vaginata 의 아미노산

김병각,이영수,최응칠,심미자,이영남 ( Byong Kak Kim,Young Soo Lee,Eung Chil Choi,Mi Ja Shim,Young Nam Lee ) 생화학분자생물학회 1977 BMB Reports Vol.10 No.1

Amanita spissacea which is indiginous to Korea and Japan and Amanita vaginata which is an edible mushroom, grow wildly in Korea. However, their constituents have never been studied. These higher fungi were collected in the Gyeong Gi Province, Korea, and analyzed for their free and total amino acids, using gas chromatography and amino acid auto-analyzer. Fourteen free amino acids (alanine, glycine, valine, leucine, isoleucine, threonine, aspartic acid, methionine, phenylalanine, histidine, tyrosine, lysine, arginine, and tryptophan) were identified in both species. The contents of eight free essential amino acids in A. spissacea and a. vaginata were : threonine 7.26 and 3.15 ㎎/g, lysine 2. 85 and 2.5 ㎎/g, methionine 2.48 and 2.70 ㎎/g, phenylalanine 1.35 and 0.98 ㎎/g, isoleucine and leucine combined 1.65 and 1.05 ㎎/g, valine 1.24 and 0.68 ㎎/g, and tryptophan 0.90 and 0.93 ㎎/g, respectively. The total contents of the respective amino acids in A. spissacea and A, vaginata were: lysine 5.18 and 12.28 ㎎/g, histidine 3.00 and 5.12 ㎎/g, arginine 7.76 and 9.80㎎/g, threonine 8.88 and 8.00 ㎎/g, glutamic acid 20.10 and 34.94 ㎎/g, alanine 10.22 and 11.62 ㎎/g, valine 9.64 and 8.94 ㎎/g, glycine 8.32 and 8.30 ㎎/g, methionine 1.90 and 1. 63 ㎎/g, isoleucine 6.66 and 7.88 ㎎/g, leucine 12.74 and 14.00 ㎎/g, phenylalanine 7.00 and 7.82 ㎎/g, cysteine 0.82 and 1.26 ㎎/g, aspantic acid 16.94 and 15.28 ㎎/g, serine 9.84 and 10.34 ㎎/g and threonine 3.62 and 3.90 ㎎/g, respectively.

한국의 고등균류의 성분 연구

김병각(Byong Kak Kim),임정한(Jeong Han Lim),윤인희(In Hee Yoon),박옥진(Ock Jin Park),김혜숙(Hae Sook Kim) 한국생약학회 1971 생약학회지 Vol.2 No.2

In order to detect poisonous or physiologically active constituents in the Higher Fungi growing in Korea, fortythree species including two varieties were collected in Kyung Ki and Chung Cheong Buk Do Provinces and subjected to systematic extraction with two kinds of organic solvents. The extracts were examined for alkaloid by adding alkaloid reagents. Of these fungi, three species, Geaster fimbriatus, Lampteromyces japonicus and Pseudocolus schellenbergiae, gave positive confirmatory tests.

한약제제의 면역학적 연구 (제1보)

김병각(Byong-Kak Kim),정경수(Kyeong-Soo Chung),최응칠(Eung-Chil Choi),김혜령(Hye-Ryung Kim),박희주(Hee-Ju Park),조필형(Pil-Hyoung Cho) 한국생약학회 1983 생약학회지 Vol.14 No.3

To examine effects of oriental pharmaceutical preparations on immunity, four of such preparations which are currently being used were examined. After their fluid extracts were injected intraperitoneally to ICR mice, the counts of total cell number, PMN and macrophage in the peritoneal cavity were made by using non-specific esterase staining method, and plaque-forming cells (PFC) were measured by using the modified method of Cunningham and Jerne. The results showed that Bo-Jung-Ik-Ki-Tang, Ih-Jung-Tang, Ohn-Kyeong-Tang and Ke-Ji-Bok-Ryeong-Hwan-Ka-Dae-Hwang increased both the macrophages and the total cells of the peritoneal cavity and that these preparations increased the number of PFC in the spleen. Therefore the data indicate that they potentiate both of the cell-mediated and humoral immunities in the mice.