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        납 , 수은 및 카드뮴이 백서 좌골신경에 미치는 영향에 관한 형태학적 연구

        정종훈,문연자,문철성,유태전,최민규,정연태 ( Chong Hoon Chung,Yeun Ja Mun,Chul Sung Moon,Tae Jeon Yoo,Min Kyu Choi,Yeun Tai Chung ) 한국환경생물학회 1991 환경생물 : 환경생물학회지 Vol.9 No.2

        In order to evaluate the effect of some heavy metals on the sciatic nerve of the rat, light and electron microscopic studies were performed to examine the fine structural changes of sciatic nerve of the rat after administration of lead nitrate, mercuric chloride and cadmium chloride, respectively. Rats were received 200㎎/ℓ of each heavy metal(Pb, Hg and Cd) solution ad libitum for 3 months and then, they were sacrificed. The results were summarized as follows; 1. Lead nitrate-treated group showed the decreased diameter of nerve fibers and axons, and mercuric chloride-treated group showed the decrement of number of nerve fibers per unit area, remarkably. 2. g ratio (d/D) measured in the heavy metal-treated groups were 0.75 in Pb, 0.82 in Hg and 0.85 in Cd respectively, compared to the 0.78 in control group. 3. Electron microscopy of lead nitrate-treated group showed the degeneration of myelin sheath, and the destructive change of membrane of Schwann cell. 4. The severe degeneration of myelin sheath and vacuoles in axon were observed in mercuric chloride-treated group. 5. In cadmium chloride-treated group, the degeneration of myelin sheath and axon, and Schwann cell were observed. 6. Degenerative changes of axon were highest in cadmium chloride-treated group(7.4%) and also those of myelin sheath were highest in mercuric chloride-treated group(23.7%) among the experimental groups. These findings indicate that lead, mercury and cadmium produce the morphological changes in the diameters of nerve fiber and axon, and thickness of myelin sheath of sciatic nerve.

      • Nicotine 이 생쥐 초기배아의 발생에 미치는 세포 독성에 관한 연구

        김길중,정종훈,박승택,최민규,정연태 圓光大學校 醫科學硏究所 1989 圓光醫科學 Vol.5 No.1-2

        This study was performed to examine the cytotoxic effect of nicotine on the various developmental stages of mouse early embryo. Two-cell embryos were cultured for 72 hours in the media containing various concentrations of nicotine. The results obtained were as follows ; 1. Two-cell embryos were completely inhibited the differentiation to blastocysts at 5 × 10 exp (-3) M nicotine concentration when two-cell embryos were cultured for 72 hours in the media containing various concentrations of nicotine. 2. The longer the embryos exposed to nicotine, the more damages appeared in mouse early embryos treated with nicotine. 3. Morphological changes such as the fusion and fragmentation of blastomeres, cytolysis and developmental retardation in embryonal cells damaged to nicotine were more increased than those of the control group dependent on time and dose. From these results, it suggests that nicotine represents cytotoxic effect on the various developmental stages of the mouse early embryos.

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