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Immunological analyses for recombinant GroEL of Brucella canis
이향근,김종완,이기찬,허문,김지연,강성일,정숙찬 한국예방수의학회 2013 예방수의학회지 Vol.37 No.1
Brucella (B.) canis is transmitted primarily by direct or indirect contact with aborted fetuses and placenta. It is also known to infect humans, which likely results in infectious risk to veterinarians and companion animal owners. For development of an effective subunit vaccine against canine brucellosis, immunogenicity for chaperonin GroEL(heat shock protein of 60kDa)of B. canis was investigated by recombinant protein vaccine, which is known to induce humoral and cellular immune responses in mouse models. Mice received subcutaneous injection in the lower back midline with 30 ug of purified GroEL on days 0, 14. Sera were obtained at 0, three and six weeks after the first immunization for detection of humoral and cellular immunity. Significantly higher IgG1 and IgG2a were detected in the sera of mice immunized with recombinant GroEL,compared to those inoculated with negative control.
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임숙경,변정열,이희수,문동찬,장금찬,정숙찬 한국예방수의학회 2014 예방수의학회지 Vol.38 No.3
The aim of this study was to investigate the prevalence of antimicrobial resistance in Escherichia coli isolated from pigs and their farmhouse environment in Korea. A total of 585 E. coli were isolated in this study during 2006∼2007: 426 isolates from 492 pigs and 159 isolates from 312 farmhouse environment samples from 16 different pig farms. The most frequently observed resistance in pig fecal samples was to older antimicrobials such as tetracycline, streptomycin, and ampicillin. However, resistance to cephalosproins, β-lactam/ β-lactam inhibitor combination, and colistin was low. We found an inverse relationship between prevalence of resistant E. coli and animal age. Resistance rate and multi-drug resistance (MDR) was greater in young pigs (piglet and nursery) than for those from adult pigs (grower-finish and sow). Resistance of E. coli from farm environment such as floor, Iron partition, and ventilation was similar with those from pig fecal samples. Farm environment contaminated with MDR bacteria might be a possible source of infections to pigs. Thus, to control and reduce the antimicrobial resistance in pigs, we must also pay attention on the environment.
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남향미,임숙경,장금찬,정대영,김휘영,이청산,정숙찬 한국예방수의학회 2013 예방수의학회지 Vol.37 No.3
During January-November 2012, a total of 2,041 quarter milk samples were collected from dairy cattle of 82 dairy farms nationwide. About 42%(870/2,041) of the samples that had somatic cell counts(SCC) of ≥200,000 cells/ml were subjected to microbiological examination. No bacteria was isolated from 95 of 870(10.9%) samples. Among 1,237 bacteria isolated from the rest 775 samples, 1,085 were identified with VITEK: more than half(52.1%, 645/1,237) of the isolates were gram negative bacillus. Gram positive cocci including Staphylococcus accounted for 35% of the isolates and almost none of gram positive bacilli isolated. Excluding Coagulase-negative Staphylococcus(CNS), the most frequently isolated bacterial species was Escherichia coli(11.2%,138/1,237), followed by Klebsiella pneumoniae(8.1%,100/1,237), Staphylococcus aureus(7.1%,88/1,237), Enterobacter cloacae(6.0%,74/1,237), Serratia marcescens(3.5%,43/1,237). The most common resistance of S. aureus was to penicillin(77.4%) and ampicillin(73.0%), while no resistance was observed against gentamicin and cephalothin. Although CNS presented resistance to all antimicrobials tested but the most prevalent resistance was to penicillin(35.6%) and ampicillin(37.0%). The pattern of antimicrobial resistance observed in CNS was similar to that of S. aureus, but the rates were much lower than those of S. aureus. E. coli also showed resistance to all the antimicrobials tested, although the rates were not very high. The highest resistance of E.coli was to cephalothin(39.4%) and ampicillin(36.2%), while most of the strains(98.0%) showed sensitivity to amikacin. The results of this study provide information on current situation of bovine mastitis in Korea.
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Rapid, sensitive and specific detection of Brucella canis by real-time PCR assay using HybProbe
강성일,김지연,이기찬,김종완,이향근,성소라,조효림,정숙찬 한국예방수의학회 2013 예방수의학회지 Vol.37 No.1
We designed and evaluated a specific HybProbe set for identification of Brucella canis, which is based on a specific single nucleotide polymorphism (SNP) of the omp25 gene. Real-time PCR assay using this probe specifically identified B. canis reference and field strains, however, the other Brucella species and general bacteria strains were not amplified. This real-time PCR assay can re-confirm specific amplification in melting curve analysis. The average melting temperature values for generation of B. canis strains was 67℃, however, that of the other Brucella species was 62℃, due to a single base-pair mismatch at the binding site of the probe. The detection limit appeared to be 30 fg of DNA and 100 CFU by serial dilution of whole blood inoculated with B. canis. In addition, use of the buffy coat resulted in approximately 50 times higher sensitivity than whole blood directly. This technique is widely applicable for use in diagnostic laboratories and is readily discriminative with regard to small amounts of biomass.
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Detection of Bartonella species from cattle ticks in South Korea
김지연,채명주,강성일,허문,구정희,이향근,이기찬,강승원,정숙찬,최서은 한국예방수의학회 2013 예방수의학회지 Vol.37 No.1
During 2010~2011, 877 ticks (Hemaphysalis longicornis and Ixodes spp.) were collected from cattle in South Korea. According to 16S RNA PCR assay for Bartonella species, 9 of them showed specific 356-bp band products. A phylogenetic analysis based on the 16S RNA showed two clusters of Bartonella strains. One cluster included four isolates that were quite similar to those previously registered on Genbank from various sources, whereas the other was highly related to the reference strain from human. It suggested that cattle ticks harbouring various Bartonella spp. might circulate among human, animals,and farm surroundings. Therefore, as ticks are main vectors that transfer pathogens affecting human beings and domestic cattle, further studies are required for bartonellosis in cattle.
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