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정기화,이광원,곽대오,강대진,박충생 ( K . H . Chung,K . W . Lee,D . O . Kwack,D . J . Kang,C . S . Park ) 한국축산학회 1991 한국축산학회지 Vol.33 No.9
To investigate the effect of PGF₂α treatment on prevention of retained placenta and on subsequent reproductive performances of induced-and spontaneous-parturient cows, 151 heads of Holstein cows raised at National Animal Breeding Institute were used. The parturition was induced by injecting 20 ㎎ of dexamethasone into cows at 5 days perior to expected calving date, and to prevent retained placenta a single dose of 10 ㎎ PGF₂α was injected i.m. within 1 hour after induced-or spontaneous-calving. The cows which did not expelled their placenta until 12 hours postparturient were termed as the cow of placenta retention. The calving of cows was induced at 45.5±2.0 hours after injection of dexamethasone. PGF₂α injection after calving to the induced-calving cows resulted in a greater reduction(P$lt;0.01) of incidence of placenta retention (27.3%)than physiologycal saline injected cows (9.09%) In days to the Ist service after calving, there was no significant difference(P$lt;0.05) between spontaneous and PGF₂α treatment after induced-calving, but physiological saline injection after induced-calving cows resulted in significantly(P$lt;0.05) longer days(106.5±27.5 days) than spontaneous calving(66.5±4.1 days). A single dose of 10 ㎎ PGF₂α to 35 spontaneous-calving cows did not significantly(P$lt;0.05) preventive effect on placenta retention (25.7%) compared with physiological saline treatment.
후산정체 (後産停滯) 가 번식효율과 산유량에 미치는 영향
정기화,조윤연,곽대오,박충생 ( K . H . Chung,Y . Y . Cho,D . O . Kwack,C . S . Park ) 한국축산학회 1991 한국축산학회지 Vol.33 No.7
To investigate the influence of retained placenta on the subsequent reproductive performance and milk yield, total 357 heads of Holstein dairy cows raised at N.A.B.I., Choong Nam province were used. The cows not expelled their placenta until 12 hours postparturient were termed as the cow of placenta retention. The days to first service. days open, services per conception, lactation days, peak yield per day. days to peak yield and 305 days ME milk yield were evaluated to determine the subsequent reproductive performances and milk yield, respectively. The mean incidence of retained placenta(RP) was 25.8% in a herd of 357 cows examined. The cows of placenta retention took significantly(P$lt;0.01) longer than normal cows in the days to first service and the days open, by 97.6±8.4 days and 192.6±15.0 days versus 79.3±2.3 days and 142.9+5.5 days respectively, but services per conception and days open more than 300 were not affected by retained placenta. In milk yield, lactation days, peak yield per day and days to peak yield were not affected by retained placenta, but milk yield per lactation was significantly(P$lt;0.05) decreased in RP cows with 5,684± 151.9㎏ versus 6,035±80.0㎏ for normal cows. From these results, there seemed to be negative relationship between incidence of retained placenta and subsequent reproductive performance and/or milk yied.
정기화(K . H . Chung),박상문(S . M . Park),곽대오(D . O . Kwack),박충생(C . S . Park) 한국축산학회 1991 한국축산학회지 Vol.33 No.6
A field experiment was performed to investigate the cause of retained placenta by season gestation length parity and sex of calf. and to determine the effect of placenta retention on the subsequent reproduction. days to first service days open and services per conception were evaluate in Holstein dairy cows. The cows which did not expell their placenta until 12 hours post-Parturient were termed as cow of Placenta retention. The time for placenta release was caleulated in the normal cows without retained placenta. The results obtained in these studies were summarized as follows; 1. The mean incidence of retained placenta was 24.4% in a total of 377 cows examined. Incidence of retained placenta was lowest in autumn calving cows(18.4%). in cows having longer than 286 days of gestation(20.0%), in 1st caking cows(14.7%) and in male calving but highest in summer calving(35.7%), in cows having shorter than 276 days of gestation(31.6%). in the 5th calving cows(28.2%) and in female calving although there were not significant differences between the respective groups. Birth weight of calves did not significantly affect the incidence of retained placenta Embryo transferred and artificial inseminated cows showed placenta retention of 33.3% and 23.9% respectively. with no significantly difference between both groups. 2. The times for placenta release in winter calving cows were significantly (P$lt; 0.05) longer than in spring and summer calving cows he 6.5±0.2 hours. but the gestation length, parity and sex of call were not found significant factors on incidence of placenta retention. 3. The period from calving to first service was significantly(p$lt; 0.01) longer in spring calving cows than autumn or winter calving ones. and also significantly(P$lt; 0.05) longer in the cows of 3rd parity than in tile cows of the 1st. 2nd, 6th and over parity. The results on days open and the services per conception showed significantly(p$lt; 0.111) lower reproductive performance in the cows of 4th parity but not significantly affected by gestation length or sex of calf. From these results. it seem to be no direct relationship between incidence of retained placenta and reproductive performance
남윤성,김남근,최명진,박상희,정기화,이숙환,윤태기,차광열,Nam, Y.S.,Kim, N.K.,Choi, M.J.,Park, S.H.,Chung, K.W.,Lee, S.H.,Yoon, T.K.,Cha, K.Y. 대한생식의학회 1998 Clinical and Experimental Reproductive Medicine Vol.25 No.3
Premature ovarian failure is a condition causing amenorrhea, hypoestrogenism, and elevated gonadotropins in women younger than 40 years. Many causes of premature ovarian failure were reported, including genetic abnormalities, enzymatic defects, defects in gonadotropin secretion or action, autoimmune disorders, physical and idiopathic causes. Recently, Finnish group reported a point mutation in the follicle stimulating hormone (FSH) receptor gene in premature ovarian failure patients. But it was reported that the group from United States could not find any mutation in FSH receptor gene. So we analysed C566T point mutation of FSH receptor gene using restriction fragment length polymorphism (RFLP) and compared the result between premature ovarian failure patient with idiopathic and known causes. But we did not find 556C${\rightarrow}$T mutation in the FSH receptor gene in both groups. These findings suggest that the missense mutation in the human FSH receptor gene reported in Finnish women with premature ovarian failure is uncommon in Korean women with premature ovarian failure.
아미노산의 첨가가 돼지 체외수정란의 후기배의 발달에 미치는 영향
김연수,송상현,조성근,곽대오,김철욱,방충생,정기화,Kim Y. S.,Song S. H.,Cho S. K.,Kwack D. O.,Kim C. W.,Park C. S.,Chung K. H. 한국동물번식학회 2005 Reproductive & developmental biology Vol.29 No.3
본 연구는 아미노산의 첨가가 돼지 수정란의 체외 발달율에 미치는 영향을 구명하고자 PEF가 함유된 NCSU-23을 기본배지로 체외성숙 및 체외배양액을 조성한 후 EA(Essential amino acid), NA(Non-essential amino acid) 및 EANA(EA+ NA)를 첨가하여 체외성숙, 체외수정 및 체외발달에 미치는 영향을 조사하였다. 체외성숙 배지에 아미노산을 첨가한 결과 MH 단계까지의 체외성숙율은 NA 첨가군이 $83.3\%$로 대조구 $70.0\%$에 비하여 유의적으로(p<0.05) 높았다. 그러나 체외 수정 이후의 배 발달율과 수정율에서는 아미노산 첨가군과 무첨가군 사이에 유의적인 차이는 없었다. 체외배양액에 아미노산을 첨가한 후 배반포의 내부세포괴(ICM) 세포와 영양배엽(TE) 세포의 발달에 미치는 영향을 조사한 결과, ICM에서는 유의차를 발견할 수 없었으나 TE 세포는 EANA 처리구가 $18.0{\pm}0.5$개로 대조구 $16.09{\pm}0.56$개에 비해 유의적(p<0.05)으로 많았다. 총세포수에서도 EANA 처리구가 $50.0{\pm}1.0$개로 대조구 $44.2{\pm}1.1$개보다 유의적(p<0.05)으로 많았다. 이상의 결과를 종합할 때, 돼지의 체외수정란 생상에 있어서 아미노산의 첨가는 배반포로의 발달율에는 영향을 미치지 못하였으나 체외성숙율을 높이고 배반포의 세포수 향상에 도움을 주는 것으로 판단된다. 특히, 영양배엽(W) 세포의 발달율이 높은 것으로 보아 아미노산의 첨가는 돼지수정란의 착상에 도움을 줄 것이라 기대된다. The objective of this study was to investigative the effects of amino acids supplementation on maturation, fertilization and embryo development of pig oocytes. Essential amino acids (EA), non-essential amino acids (NA) or both amino acids (EA + NA) were supple-mented to North Carolina State University (NCSU) 23 medium containing porcine follicular fluid (pFF). When the amino acids were supplemented to the maturation medium, the maturation rates were higher (p<0.05) in the NA group than control ($83.3{\pm}0.04\%\;versus\;70.0{\pm}0.05\%$, but the subsequent cleavage rates and development to morula and blstocyst stage between aminoacid supplement groups and control were not different. The developmental rates to morula and blastocysts stage were not significantly different regardless of amino acid supplementation to culture medium. In addition, supplementation of amino acids did not significantly affect the rate of fertilization and polyspermy. When the amino acids were supplement to culture medium, the number of trophectodermal (TE) cells was significantly (p<0.05) higher in amino acid supplement group than that of control ($18.6{\pm}0.5\;versus\;16.1{\pm}0.6$), whereas the numbers of inner cell mass (ICM) cells were not different among the treaonent groups and control ($29.0{\pm}0.9\~31.5{\pm}1.2$). Total cell number was also significantly (p<0.05) higher in EANA group ($50.0{\pm}1.0$) than that of control group ($44.2{\pm}1.1$). These results indicate that the amino acid supplementation to maturation and culture medium may not significantly stimulate early embryo development, but may improve the TE cell number of blastocyst stage in the pig.
안병석(B . S . Ahn),김내수(N . S . Kim),정기화(K . H . Chung),오대균(D . K . Oh),강만석(M . S . Kang) 한국축산학회 1990 한국축산학회지 Vol.32 No.5
This study was carried out to determine the effects of body measurements and weights of dams on calves body weights in Korean native cattle raised at National Animal Breeding Institute from 1981 to 1988. The data obtained from the records for 194 heads of cows and 185 heads of calves. For calf body weight at birth, the effect of dam parity on male calf was significant at 1% level. The effect for calf weight at 3 months was not significant, but the effect for calf weight at weaning was significant at 1% level on the female and the male. On the phenotypic correlations of body weights and measurements of dams on calves weights at birth the body weight of the female on 3rd parity were positively correlated with chest width, hip width and thurl width of dam. On the Ist parity, body weight of the male at 3 month were positively correlated with widthers height, chest girth, chest widths, hip width, thurl width, body weight of dam. But those of weaning weights of calves with body measurements and weights of dam did not showed uniform trend.