포장방법에 따른 한우 극상근(Supraspinatus Muscle)의 냉장 저장 중 품질 변화

정구용,정의룡,이현정,Chung, Ku-Young,Chung, Eui-Ryung,Lee, Hyun-Jung 한국축산식품학회 2007 한국축산식품학회지 Vol.27 No.4

본 연구에서는 한우 앞다리살 부위육인 극상근(Supraspinatus m.)의 포장방법(랩포장, 진공포장 및 가스포장)에 따른 냉장 저장 중 품질변화를 살펴보았다. 포장방법에 따른 pH 변화는 차이가 없었으며, VBN가는 가스포장구의 경우 저장 25일에도 17.65 mg%로 다른 포장구에 비해 낮은 수준이었다. 포장감량은 진공포장구, 랩포장구, 가스포장구의 순으로 높았으며, 육색은 가스포장구가 저장기간 동안 안정적 육색 값을 나타내었다. 경도는 포장구 중 가스포장구의 경도가 저장 15일에 1.74 kg으로 랩포장구의 2.57 kg 보다 낮았다. 총균수는 랩포장구에서 저장 20일에 8.50 log CFU/g로 가식권에 벗어났으나, 가스포장구의 경우 저장 25일에도 7.41 log CFU/g로서 다른 포장구에 비해 다소 저장성이 지연됨을 알 수 있다. 또한 진공포장구와 가스포장구는 랩포장구에 비하여 낮은 대장균군수를 나타내었다. 따라서 한우 극상근을 냉장 저장할 경우 가스포장 하는 것이 극상근의 품질 변화 없이 연도를 증진시키는 방법 이라 사료된다. The aim of this study was to investigate the quality changes in Supraspinatus muscle of Hanwoo with different packaging methods, such as wrapped packaging (C), vacuum packaging (V) and modified atmosphere packaging (MA). These samples were stored for 25 days under $5^{\circ}C$. Each sample was evaluated for pH, volatile basic mitrogen (VBN) value, purge loss, hardness, meat color and microbial counts. From the results obtained, no significant differences were observed among treatments at the pH. The VBN values of MA were significantly lower than those of C. Also, MA was enhanced stability of meat color compared with other packaging methods during storage days. The purge loss was the most in the V samples, followed by the C and the MA was the lowest. Total bacteria counts of MA sample were significantly lower than those from C. Therefore, it has been concluded that MA could be used as an effective packaging since it extends the shelf life and improve the quality of Hanwoo supraspinatus m.

친환경 유기농 채소가 첨가된 저지방 햄버거 패티의 제조

정구용,정의룡,이주연,Chung, Ku-Young,Chung, Eui-Ryung,Lee, Joo-Yeon 한국축산식품학회 2008 한국축산식품학회지 Vol.28 No.2

In this study, the physicochemical, microbial, and sensory properties of law-fat hamburger patties during storage for 25 days at $7{\pm}1^{\circ}C$ were investigated. The law-fat hamburger patties were manufactured by three different packing methods (control: added with 10% lard regular-fat patty, T1: added with 10% lard and organic vegetable of regular-fat patty, T2: added with 3% olive oil of vegetability low-fat patty, T3: added with 3% lard of low-fat patty). The pH of all treated samples increased as the storage time increased, and then decreased after 15 days of storage. The low-fat hamburger patty added with organic olive oil (T2) showed significantly higher pH (p<0.05) compared with other treatments (T1 and T3). The TBARS values of the all treated samples tended to increase after 5 days of storage, and then significant quality loss was observed after 15 days of storage period for the control (T1). However, the samples of the vegitability low-fat patty added organic olive oil had longer shelf-life than the control. The total bacterial counts were 7 log CFU/g after 15 days and 20 days of storage for the control and treatments, respectively. The results of this study showed that the storage period of the treatments was slightly extended compared with the control. Low-fat hamburger patties showed no differences for overall acceptability between control and other patties.

신장 허혈 재관류 손상에 미치는 Ethyl Pyruvate의 효과

정구용(Ku-Yong Chung),정규영(Gyu Young Jeong),안형준(Hyung Joon Ahn),주만기(Man Ki Ju),장혜경(Hye Kyung Chang),이우정(Woo Jung Lee),한평림(Pyung-Lim Han),김유선(Yu Seun Kim) 대한외과학회 2007 Annals of Surgical Treatment and Research(ASRT) Vol.72 No.5

Purpose: Reactive oxygen species (ROS) significantly contribute to ischemia-reperfusion injury, and are also associated with the gradual loss of renal function and renal failure following renal transplantation. Pyruvate is an endogenous antioxidant, but its use as a therapeutic agent for treating conditions mediated by oxidative stress is limited due to its poor stability in solution. However, ethyl pyruvate (EP), a soluble pyruvate derivative, has far greater stability than pyruvate; thus, may serve as a practical pyruvate precursor. Therefore, the ability of EP in the prevention of renal ischemia- reperfusion injury was assessed. Methods: Sprague-Dawley rats (n=54) were subjected to 40 minutes of renal warm ischemia. The animals were divided into three groups: the sham group without warm ischemia (n=18), the EP group (n=18, EP given before ischemia), and the ischemic control (n=18). The serum levels of creatinine and TNF-α were measured 1, 3 and 5 days after induction of ischemia. The expression of high mobility group box-1 (HMGB-1), a delayed inflammatory mediator, was also assessed by Western blot of renal specimens. Results: In the EP group, late improvements in the serum levels of creatinine and TNF-α were observed in comparison with the ischemic control. Based on this delayed effect, the expression of HMGB-1 was assessed in renal tissue. The HMGB-1 expression increased over time during the ischemia process, but EP suppressed this expression 3 and 5 days after renal ischemia-reperfusion injury. Conclusion: These results have demonstrated, for the first time, that EP ameliorates renal ischemia-reperfusion injury. EP attenuates the renal ischemia-reperfusion injury, at least in part, by suppressing the expression of HMGB-1, a late mediator of delayed inflammation.

개의 동종 부분 간이식 후 허혈

정구용(Ku-Yong Chung),성순희(Soon Hee Sung),정규영(Gyu Young Jeong),오수연(Soo Youn Oh),김유선(Yu Seun Kim),조백환(Baik-Hwan Cho),최금자(Kum Ja Choi),최용만(Yong Man Choi) 대한외과학회 2002 Annals of Surgical Treatment and Research(ASRT) Vol.63 No.5

단보 : 살라미 외피로부터 분리한 곰팡이의 동정

유영현 ( Young Hyun You ),김대호 ( Dae Ho Kim ),정구용 ( Ku Yong Chung ),홍승범 ( Seung Beom Hong ) 한국균학회 2014 韓國菌學會誌 Vol.42 No.1

The fungi play an important role in smell and taste during the fermentation of salami. In this study, the fungi from the salami casing during the fermentation process in Korea were isolated. They were examined by morphological and molecular methods and were identified as Aspergillus cibarius, Penicillium echinulatum, Cladosporium sphaerospermum. Of these, A. cibarius formed white or light green conidial heads on salami casing and it was widely distributed through almost whole casing. P.echinulatum occurred sparsely on the casing of the salami and formed white masses. C. sphaerospermum occurred rarely and formed black spots. It is considered that additional studies on their roles on salami are required.

웅성 특이적 SRY 및 ZFY 유전자를 이용한 쇠고기 성(性) 판별

신성철,정구용,정의룡,Shin, Sung-Chul,Chung, Ku-Young,Chung, Eui-Ryong 한국축산식품학회 2007 한국축산식품학회지 Vol.27 No.3

본 연구는 포유류의 Y-염색체상에 존재하는 SRY 및 ZFY의 웅성 특이적 성 결정 유전자를 이용하는 PCR 기법으로 쇠고기 성판별 기술을 개발하고자 수행하였다. 성 결정 유전자 영역의 특정 염기서열을 포함하는 primer를 각각 설계 합성하고 이들 primer를 이용하여 PCR증폭을 실시한 다음, 각각의 증폭산물을 1.5% agarose gel에 전기영동하여 웅성 특이적 DNA band의 증폭여부를 확인하였다. SRY 유전자에서 웅성개체 쇠고기는 1,348 bp 크기의 단편을 가진 DNA band가 검출되었으나, 자성개체의 경우 DNA band가 전혀 검출되지 않은 것을 확인 할 수 있었다. 또한, ZFY 유전자에서 웅성개체의 쇠고기는 979 bp 크기의 단편을 가진 DNA band가 모두 검출되었으나, 자성개체의 쇠고기에서는 역시 DNA band가 전혀 검출되지 않았다. 즉, SRY 및 ZFY 유전자는 모두 숫소에서 유래한 쇠고기에서 웅성 특이적인 DNA band가 정확히 검출된 반면 암소에서 유래한 쇠고기에서는 웅성 특이적 DNA band가 전혀 검출되지 않았다. 또한 쇠고기 성 감별법을 도축 후 가공 유통판매단계에 실제 활용 가능성을 검증하고자 시중에 유통되고 있는 등심 및 갈비 포장육 350시료를 무작위 추출하여 성 판별을 실시한 결과 숫소가 252시료(72%) 그리고 암소가 98두(28%)로 조사되었다. 따라서 본 연구에서 개발한 SRY 또는 ZFY의 웅성 특이적 성 결정 유전자를 이용하는 쇠고기 성 감별기술은 생산단계는 물론 도축 후 가공 유통 판매되고 있는 모든 쇠고기의 암수 성감별을 위한 유용한 DNA 표지인자로 활용할 수 있을 것으로 기대된다. The objective of this study was to develop a rapid and reliable method for the sex determination of beef using the PCR(polymerase chain reaction) technique. We have used two bovine sex determining genes, SRY and ZFY, on the Y-chromosome to identify the sex of Hanwoo and Holstein beet. We attempted to amplify 1,348 bp and 979 bp fragments from male and female genomic DNA corresponding to the SRY and ZFY genes, respectively, using male specific primers. The amplified PCR products were separated by electrophoresis in a 1.5% agarose gel to detect a male specific DNA band. When DNA from male beef was amplified with primers specific for the SRY gene, a DNA band of 1,348 bp was present in all of the male samples, but absent from all of the female samples. Also, when DNA from male beef was amplified with primers specific for the ZFY gene, a DNA band of 979 bp was observed in all of the male samples, but absent from all female samples. In conclusion, the bovine SRY and ZFY genes are typically found only in male beef. For the practical application of this method for the sexing of commercial beef at the processing and marketing stages after slaughter. a total of 350 beef samples collected randomly from local markets were analyzed for sex determination. The proportions of male and female samples were 252 (72%) and 98 (28%), respectively. Therefore. the SRY and ZFY genes. which are specific for the Y-chromosome, may be useful sex-diagnostic DNA markers to distinguish male meat from female meat.

모색 발현 유전자의 DNA Marker를 이용한 쇠고기 품종 판별

정의룡,정구용,Chung Eui-Ryong,Chung Ku-Young 한국축산식품학회 2004 한국축산식품학회지 Vol.24 No.4

본 연구는 축우의 모색발현에 관여하는 MC1R, MGF 및 TYRP1 3종류의 모색 유전자의 PCR-RFLP marker를 이용하여 쇠고기 품종 판별기술을 개발하고자 수행하였다. MC1R 유전자의 104번째 아미노산을 지정하는 codon에 GGT 염기를 갖고 있는 Holstein 젖소와 Angus 육우는 제한효소 인지부위가 존재하여 537 bp증폭산물이 절단되어 329와 208bp 두개의 band가 검출되었으나 한우에서는 GTG로 G 염기가 T염기로 치환됨으로써 제한효소 인식부위가 소실되어 537 bp의 단일 bind 만이 검출되었다. 따라서, 이처럼 MC1R 모색유전자의 품종 간 특정 염기서열의 차이가 곧 특정 제한효소의 염기 서열상의 인지 부위 차이를 가져와 한우와 Holstein 젖소 및 Angus 육우 품종간의 RFLP 유전자형 출현에 확실한 차이가 인정되어 한우 품종에 특이적인 MC1R 유전자의 RFLP marker를 이용한 한우육 판별이 가능하였다. 또한, MGF 유전자의 RFLP 유전자형 출현빈도에서 한우는 r/r형이 75%로 출현율이 매우 높은 유전자형으로 분석된 반면 Hereford종은 R/R 형이 80%로 출현율이 매우 높았고 Holstein종과 Angus종은 R/r형이 100% 출현함으로써 한우와 Holstein 및 수입육우 품종간의 MGF 유전자형 출현빈도에 뚜렷한 차이가 인정되었다. 한편, TYRP1 유전자의 RFLP유전자형을 분석한 결과 모든 품종에서 동일한 RFLP type이 검출되어 TYRP1 모색 유전자를 이용한 쇠고기 품종 구별은 불가능한 것으로 나타났다. 따라서, 소 모색 관련 MC1R과 MGF 두 유전자의 품종 특이적 PCR-RFLP 유전자형은 한우육과 국내산 Holstein젖소고기 및 Angus 수입육간의 품종을 식별하는데 매우 유용한 DNA marker로 이용될 수 있음이 확인되었다. In Korean beef market, one of the major problems is mislabeling or fraudulent distribution of Holstein dairy meat or imported beef as domestic Hanwoo meat. Therefore, there has been a great need for a development of technology to identify beef breeds in meat and meat products. This study was carried out to develop the accurate and reliable method for the identification of beef breed using PCR-RFLP marker of MC1R, MGF and TYRPl genes affecting coat colors in cattle. A single base substitution (G\longrightarrowT transition) at the codon for amino acid position 104 of MC1R gene was identified between Hanwoo and Holstein and Angus breeds. The change at this position creates Msp I restriction site in Holstein and Angus, but not in Hanwoo. When the DNA amplified products (537 bp) was digested with Msp I, Hanwoo meat showed a single band of 537bp, while two fragments of 329bp and 208 bp were observed in Holstein meat and Angus breed, respectively. Thus, breed-specific RFLP marker in the MC1R gene can be used to distinguish between Hanwoo meat and Holstein and Angus meats. In the RFLP genotype of MGF gene, the frequency of r/r type was 75% in Manwoo, whereas the frequency of R/R was 80% in Hereford breed. Holstein and Angus breeds showed 100% for R/r type. Therefore, Hanwoo meat showed significant difference in the MGF genotype frequencies compared with those of Holstein meat and imported beef cattle breeds. However, TYRP1 gene showed the same genotype in all breeds examined. Thus, this TYRP1 gene can not be used as a molecular marker for breed identification. As a consequence, we suggest that RFLP markers of the MC1R and MGF coat color genes could be used as DNA marker for identification of Hanwoo meat from Holstein and imported meats.

DNA검사기법을 이용한 PSE 돈육 생산 돼지 진단

정의룡,정구용,Chung Eui-Ryong,Chung Ku-Young 한국축산식품학회 2004 한국축산식품학회지 Vol.24 No.4

돼지 골격근 근소포체의 $Ca^{2+}$ 방출통로(calcium - release channel)를 지정하는 ryanodine receptor (RYR1) 유전자의 이상은 악성고열증(malignant hyperthermia, MH)을 유발하고, RYR1 유전자의 점 돌연변이는 돼지 스트레스 증후군(porcine stress syndrome, PSS)과 밀접하게 관련되어 있다. PSS 유전인자 보유 돼지의 90% 이상은 PSE 돈육을 생산하는 것으로 알려져 있어 물퇘지 발생과 생산성 하락으로 경제적 손실을 초래하는 유전적 원인의 PSS 유전자를 검사하여 제거하는 것은 고품질 돼지고기 생산 및 국내 양돈산업의 경쟁력 향상에 매우 중요한 과제라고 할 수 있다. 따라서, 본 연구는 PCR-RFLP 및 PCR-SSCP 기법을 이용하여 PSE 돈육을 생산 하는 PSS 돼지 유전자 진단기술을 개발하고 이를 이용한 국내 종돈 및 교잡 비육돈의 PSS 유전자형 출현빈도를 파악하고자 수행하였다. 돼지 PSS의 원인이 되는 RYR 유전자의 단일염기 돌연변이 (RYR1 C1843T)를 포함하는 DNA 영역을 PCR로 증폭한 후 RFLP 및 SSCP 기법을 이용하여 분석한 결과 동형접합체의 정상 개체(N/N), 이형접합체의 잠재성 개체(N/n)그리고 열성의 돌연변이 유전자를 동형접합체 상태로 갖는 PSS 감수성 개체(n/n)에 각각 특이적인 RFLP 및 SSCP 유전자형이 검출되어 PSS 저항성, 잠재성 및 감수성 개체의 정확한 판별이 가능하였다. 돼지 주요 품종 집단내 PSS유전자형 출현빈도를 조사한 결과 Landrace는 PSS저항성 개체가 57.1%, 잠재성 개체가 35.7%그리고 PSS 감수성 개체의 출현 비율은 7.1%로 분석되었고 L. Yorkshire는 82.5, 15.8 및 1.7%, Duroc은 95.2, 4.8 및 0.0%로 각각 조사되었다. 비육용 교잡돈은 정상 개체가 72.0%, 잠재성 개체가 22.7% 그리고 PSS 감수성 개체는 5.3%였다. 특히, PCR-SSCP 기법을 이용한 RYR1 유전자 돌연변이 검출 방법은 보다 신속 간편하면서도 상대적으로 분석비용이 저렴한 정확성이 높은 PSS 돼지 진단기술로서 대규모 돼지집단 검색이나 RFLP 방법으로 판정이 불확실한 시료의 재검에 효율적으로 이용할 수 있을 것으로 판단된다. Stress-susceptible pigs have been known as the porcine stress syndrome (PSS), swine PSS, also known as malignant hyperthermia (MH), is characterized as sudden death and production of poor meat quality such as PSE (pale, soft and exudative) meat after slaughtering. PSS and PSE meat cause major economic losses in the pig industry. A point mutation in the gene coding for the ryanodine receptor (RYR1) in porcine skeletal muscle, also known calcium (Ca$^{2+}$) release channel, has been associated with swine PSS and halothane sensitivity. We used the PCR-RFLP(restriction fragment length polymorphism) and PCR-SSCP (single strand conformation polymorphism) methods to detect the PSS gene mutation (C1843T) in the RYR1 gene and to estimate genotype frequencies of PSS gene in Korean pig breed populations. In PCR-RFLP and SSCP analyses, three genotypes of homozygous normal (N/M), heterozygous carrier (N/n) and homozygous recessive mutant (n/n) were detected using agarose or polyacrylamide gel electrophoresis, respectively. The proportions of normal, carrier and PSS pigs were 57.1, 35.7 and 7.1% for Landrace, 82.5, 15.8 and 1.7% far L. Yorkshire, 95.2, 4.8 and 0.0% for Duroc and 72.0, 22.7 and 5.3% for Crossbreed. Consequently, DNA-based diagnosis for the identification of stress-susceptible pigs of PSS and pigs producing PSE meat is a powerful technique. Especially, PCR-SSCP method may be useful as a rapid, sensitive and inexpensive test for the large-scale screening of PSS genotypes and pigs with PSE meat in the pork industry.y.

Nitric Oxide Donor가 신장 허혈 재관류 손상 시 내인성 Endothelin-1 분비에 미치는 영향

정규영(Gyu-Young Jeong),성순희(Sun Hee Sung),김상이(Sang Yee Kim),손정은(Jeong Eun Sohn),이우정(Woo Jung Lee),김유선(Yu Seun Kim),정구용(Ku-Yong Chung) 대한외과학회 2004 Annals of Surgical Treatment and Research(ASRT) Vol.66 No.3

한우 아포지단백질 E (APOE) 유전자의 SNP Marker가 육량 및 육질형질에 미치는 영향

신기현,신성철,정구용,정의룡,Shin, Ki-Hyun,Shin, Sung-Chul,Chung, Ku-Young,Chung, Eui-Ryong 한국축산식품학회 2009 한국축산식품학회지 Vol.29 No.1

Apolipoprotein E (APOE) is a plasma lipoprotein in mammals and plays an important role in the transport and metabolism of lipids such as phospholipids and triglycerides. Therefore, the APOE gene could be a candidate gene controlling lipid metabolism in beef cattle. This study was performed to identify single nucleotide polymorphisms (SNP) in the APOE gene and to investigate the effects of SNP genotype on the carcass traits such as meat quantity and quality in Korean cattle. For PCR amplification, pooled DNA made from unrelated 60 individuals was prepared and primer pairs were designed based on the cDNA sequence of exon 4 region of the bovine APOE gene. A SNP was identified at position 2034 (T/C substitution) of the exon 4 region in the APOE gene. PCR-RFLP procedure with restriction enzyme ACC I was developed for determining the SNP genotype for each of a total of 309 animals with pedigree information and performance records through the national progeny testing program. The frequencies of the genotypes TT, TC and CC were 10.9, 46.9 and 42.2%. Gene frequencies were 0.344 for T allele and 0.656 for C allele. The g.2034T>C SNP genotype showed a significant effect (p<0.05) on dressing percentage and meat color, respectively. Animals with the TT genotype showed higher dressing percentage than those with the CC genotype, and TT genotype had desirable meat color compared with CC genotype. These results suggest that the g.2034T>C SNP genotype of the APOE gene may be useful as a DNA marker for meat quantity index and dressing percentage in Korean cattle.