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증례 : 감염 ; 뇌사를 초래한 Salmonella Enterica Serovar Enteritidis 균혈증 1예
오대명 ( Dae Myung Oh ),권기태 ( Ki Tae Kwon ),배수현 ( Soo Hyun Bae ),이미정 ( Mi Jung Lee ),설수환 ( Soo Hwan Seol ),도원석 ( Won Seok Do ),전병권 ( Byoung Kwon Jun ) 대한내과학회 2012 대한내과학회지 Vol.82 No.5
We present a case report of brain death caused by Salmonella enterica serovar Enteritidis bacteremia. A 53-year-old diabetic male was admitted to our hospital with stupor mentality and fever. No evidence of meningitis was found upon cerebrospinal fluid analysis, but diffuse brain edema was shown by magnetic resonance imaging. S. Enteritidis phage type 7 was isolated from blood cultures. We diagnosed the patient with encephalopathy associated with S. Enteritidis bacteremia and treated him by intravenous administration of ceftriaxone and dexamethasone. Fifteen days after admission, the patient was diagnosed with brain death and became a cadaveric donor. Although encephalopathy associated with S. Enteritidis bacteremia is rare, it should be considered as a serious and potentially lethal complication. (Korean J Med 2012;82:637-641)
전병권,박성대,임시규,박완 한국산업미생물학회 1993 한국미생물·생명공학회지 Vol.21 No.5
대장균의 온도 감수성 형태형성 변이주 TMM 23(mrdAts)와 TMM 24(mrdBts)는 형태 변이가 일어나는 고온조건하에서 특이적으로 sarkosyl, SDS, Na-DOC와 같은 음이온성 계면활성제에 의해 생육이 저해되었으며 그 작용 약식은 살균적이었다. 각 균주의 변이형에 대한 sarkosyl의 최소생육저지종도 및 최소사멸농도는 동일하였으며 TMM 23에 대해서는 0.1%, TMM 24의 경우는 0.3%이었다. 생육저해작용이 없는 중성 계면활성제 Triton X-100에 의해서는 형태변화에 관계없이 생세포로부터 단백질이 거의추출되지 않았으나, sarkosyl에 의해서는 TMM 23(mrdAts), TMM 24(mrdBts) 모두, 변이형인 구균형의 생세포는 야생형인 간균형에 비해 맣은 양의 단백질이 추출되었다. 또, SDS-polyacryamide gel 전기영동에 의한 막단백질의 분석 결과, 간균형 세포의 외막에 다량 존재하는 주요 외막 단백질들이 구균형의 세포에서는 소량 밖에 보이지 않았으며, 내막의 여러 단백질의 조성의 변화도 관찰되었다. 이는 형태형성 변이주의 막구조에 물리 화학적인 성질의 차이가 있음을 나타내는 것으로 대장균 세포의 형태형성 기구는 peptidoglycan 뿐만이 아닌 내, 외막을 포함하는 세포표층의 전 구조체가 관여하고 있을 가능성을 시사하고 있다. Escherichia coli FmrdAts and mrdBts mutants forming spherical cells at 42℃, were employed to investigate the possible role of both inner and outer membrane structures in the determination of cell shape of gram-negative cells. Spherical cells, but not rod-shaped wild types, were specifically killed by anionic detergents, such as sarkosyl, sodium dodecylsulfate and sodium deoxycholate. From the spherical intact cells grown overnight at 42℃, much more proteins were released by sarkosyl. However, nonionic detergent like Triton X-100 which showed no inhibition of growth of spherical cells extracted little amounts of proteins from spherical intact cells as well as from rod-shaped cells. Analyses of membrane fractions of spherical cells by SDS-polyacrylamide gel electrophoresis showed that there were apparently reduced amounts of major outer membrane proteins and also some changes in the pattern of inner membrane proteins. Based on these results showing the differences in physicochemical properties of the membrane structures of spherical and rod-shaped cells, we suggest that the determination of cell shape in E. coli cells might be associated with both outer and inner membrane structures along with peptidoglycan.
이진숙,박완,전병권,임시규,김정옥 경북대학교 유전공학연구소 1992 遺傳工學硏究所報 Vol.7 No.1
Cell envelopes prepared from the spherical mutant strains mrdA and mrd B of Escherichia coli were characterized on the basis of major outer membrane proteins content. As compared with those of rod shaped cells of wild type, the amount of major outer membrane proteins of the spherical mutants were decreased on the SDS-PAGE analysis. And the spherical cells were sensitive to anionic detergents whereas the rod shaped cells were resistant. These results suggest that outer membrane structure, in addition to peptidoglycan layer, may also correlated with determination of cell shape in Escherichia coli.
박성대,박완,전병권,임시규 경북대학교 유전공학연구소 1995 遺傳工學硏究所報 Vol.10 No.1
Escherichia coli mrdAts and mrdBts mutants forming spherical cells at 42℃, were employed to investigate the possible role of both inner and outer membrane structures in the determination of cell shape of gram-negative cells. Spherical cells, but not rod-shaped wild types, were specifically killed by anionic detergents, such as sarkosyl, sodium dodecylsulfate and sodium deoxycholate. From the spherical intact cells grown overnight at 42℃ , much more proteins were released by sarkosyl. However, nonionic detergent like Triton X-100 which showed no inhibition of growth of spherical cells extracted little amounts of proteins from spherical intact cells as well as from rod-shaped cells. Analyses of membrane fractions of spherical cells by SDS-polyacrylamide gel electrophoresis showed that there were apparently reduced amounts of major outer membrane proteins and also some changes in the pattern of inner membrane proteins. Based on these results showing the differences in physicochemical properties of the membrane structures of spherical and rod-shaped cells, we suggest that the determination of cell shape in E. coli cells might be associated with both outer and inner membrane structures along with peptidoglycan.