산마늘 추출물의 항산화활성

장준복 ( Jun Pok Chang ),도은수 ( Eun Soo Doh ),길기정 ( Ki Jung Kil ),양재경 ( Jae Kyung Yang ),윤충원 ( Chung Weon Yun ),이건희 ( Gun Hee Lee ),정윤혜 ( Yun Hae Jung ),지윤선 ( Yoon Sun Ji ),김보람 ( Bo Ram Kim ),최명석 ( Myung 한국임학회 2011 한국산림과학회지 Vol.100 No.3

울릉도 자연산 및 강릉산 재배 산마늘(Allium victorialis var. platyphyllum)의 물 추출물과 70% 에탄올 추출물의 항산화 활성을 조사하였다. 총 폴리페놀 함량은 강릉산보다 울릉도산에서, 인경보다 잎에서 높게 나타났으며, 강릉산 잎의 70% 에탄올 추출물에서 52.57 mg/g으로 가장 높게 나타났다. 플라보노이드 함량은 잎의 에탄올 추출물에서 유의성 있게 높게 나타났으며, 인경에서는 산지별, 추출용제별 차이가 없었다. 울릉도산 잎의 70% 에탄올 추출물에서 73.30 mg/g으로 가장 높게 나타났다. 전자공여능은 울릉도산은 70% 에탄올 추출물에서, 강릉산은 물 추출물에서 높았고, 인경보다 잎에서 높게 나타났다. 울릉도산 잎의 알코올 추출물에서 55.13%로 가장 높게 나타났다. SOD 유사활성은 강릉산보다 울릉도산에서 높게 나타났고, 울릉도산 잎의 에탄올 추출물이 92.00%로 가장 높게 나타났다. Hydroxy radical 소거활성은 잎의 에탄올 추출물에서, 인경보다는 잎에서, 울릉도산 보다는 강릉산 잎에서 높게 나타났다. 강릉산 잎의 70% 에탄올 추출물에서 58.85%로 가장 높았다. 지질과산화 억제활성은 울릉도산과 강릉산 모두 잎과 에탄올 추출물에서 높게 나타났고, 울릉도산 잎의 70% 에탄올 추출물에서 73.33%로 가장 높았다. 본 연구 결과로 산마늘 추출물은 항산화 관련 건강기능성 식품 등으로 널리 응용될 수 있을 것이다. Leaf and bulb of wild garlic (Allium victorials var. platyphyllum) from Ulleung Island and Gangneung region were extracted with water and 70% ethanol and investigated on its antioxidative activity. Total polyphenol content of Ulleung island wild garlic was higher than that of Gangneung region. Total polyphenol content in bulb was high compared to content of the leaves, and 70% ethanol extract of Ulleung Island leaf was high in 72.50 mg/g. Flavonoid content in leaf was higher than that of bulb, 70% ethanol extract of Ulleung Island leaf was high in 73.30 mg/g. Electron donating activity of 70% ethanol extract from Ulleung island and water extracts (55.13%) from Gangneung were higher than those of other extracts. Bulb extracts on electron donating activity were higher than those of the leaf extracts. SOD like activity of extracts was high in 70% ethanol extract of wild gallic leaf cultivated at Gangneung. Hydroxy radical scavenging activity of wild gallic was high in leaf extracts compared to activity of bulb extracts. Hydroxy radical scavenging activity (58.85%) of Ulleung island wild gallic leaf extracts was higher than that of the wild gallic leaves of Gangneung. Lipid peroxidation inhibitory activity was both high in water and 70% ethanol leaf extracts of Ulleung island and Gangneung region, especially, 70% ethanol extract of leaves from Ulleung island was the highest 73.33%. These results suggest that extracts from wild garlic could be used as an antioxidative functional food source.

조릿대의 부위별 추출물의 항산화 활성

강준우 ( Jun-woo Kang ),장준복 ( Jun-pok Chang ),유지현 ( Ji-hyun Yoo ),도은수 ( Eun-soo Doh ),길기정 ( Ki-jung Kil ) 대한본초학회 2016 大韓本草學會誌 Vol.31 No.6

Objectives : This study was performed to investigate the antioxidant activity of water and ethanol extracts from Sasa borealis leaves, stems and roots. Methods : Sasa borealis leaves, stems and roots extract were prepared using water and ethanol. The total polyphenol and total flavonoid contents were analyzed. 2,2-diphenyl-1-picrylhydrazyl(DPPH) radical scavenging activity, SOD like activity, 2,2`-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid)(ABTS), hydroxyl radical scavenging activity, and Nitrite scavenging activity assays were carried out to determine the antioxidant activities. Results : The antioxidant activities of the Sasa borealis appeared higher in ethanol extract than water extracts. Total polyphenol and total flavonoids contents in ethanol extracts of leaves were 24.6 ㎎/㎖ and 14.3 ㎎/㎖, respectively, which were much higher than those of any other parts. SOD like activity was 70% ethanol extract of the leaves was highest with 15.68%. Electron donating ability was 70% ethanol extract of the leaves had the highest 59.07%. It exhibited high electron donating ability than BHT(45.68%). Nitrite scavenging activity of 70% ethanol extract was higher than the water extract at pH 2.5 and pH 4.2. Nitrite scavenging activity was 70% ethanol extract of the leaves was the highest 75.2%. Hydroxyl radical scavenging activity was 70% ethanol extract of the leaves was highest with 16.16%, showed very low activity than BHT(61.56%). Conclusions : These results suggest that 70% ethanol extracts from Sasa borealis leaves, exhibited higher antioxidant activities than those of root and stem, and can be potentially used as proper natural antioxidants.

쉽싸리 부위별 물추출물의 항산화 활성

송윤진 ( Youn-jin Song ),장준복 ( Jun-pok Chang ),유지현 ( Ji-hyun Yoo ) 대한본초학회 2016 大韓本草學會誌 Vol.31 No.6

Objectives : This study was performed to investigate the antioxidant activity of water extracts from Lycopus lucidus Turcz. ex Benth. leaves, stems and roots at the 100 ㎍/㎖ concentration. Methods : The different part of Lycopus lucidus Turcz. ex Benth. extract was prepared using water. The antioxidant activities of polyphenol contents, total flavonoid contents, DPPH(1,1-diphenyl-2-picrylhydrazyl) free-radical scavenging activity, SOD like activity, hydroxyl radical, ABTS(2,2`-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid), Fe2+ chelating, and nitrite scavenging activity. Results : The total polyphenol and total flavonoid content of leaves were the highest at 221.85 ㎍/㎎ and 794.13 ㎍/㎎, respectively. Electron donating ability was the 79.68% in the water extract from leaves. The ABTS radical scavenging activity of the hot extracts, leaves ≫ roots > stems was higher in the order. It was shown the highest at 94.53% in the water extract from leaves, which showed a value equal to 94.7% of ascorbic acid. The hydroxyl radical scavenging activity was the highest at 8.07% in the water extract from leaves. SOD like activity and Fe2+chelating activity were leaves of 12.3% and 27%, respectively, which were much higher than those of any other parts. The nitrite scavenging ability of extracts was increased at pH 2.5, and those was the highest in leaves of 83.03%. Its were more than twice the 41.61% of BHT. Conclusion : The results suggest that Lycopus lucidus Turcz. ex Benth. can be used as nutraceutical foods and natural antioxidant.

추출용매에 따른 다릅나무 수피 추출물의 항산화 활성

김경선 ( Gyeong-sun Kim ),장준복 ( Jun-pok Chang ),도은수 ( Eun-soo Doh ),길기정 ( Ki-jung Kil ),유지현 ( Ji-hyun Yoo ) 대한본초학회 2016 大韓本草學會誌 Vol.31 No.3

Objectives : The objective of this research was to investigate the antioxidant activities of stem bark of Maackia amurensis extract. Methods : Stem bark of Maackia amurensis extract were prepared using 70% methanol. Methanol extracts were fractionated to hexane, chloroform, ethyl acetate, butyl alcohol, water fractions and investigated. The antioxidant activities of fractions was evaluated by four different assays as total polyphenol contents, total flavonoid contents, DPPH(1,1-diphenyl-2-picrylhydrazyl) free-radical scavenging activity and ABTS(2,2``-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) scavenging ability. Results : The yield of methanolic extracts from stem bark of Maackia amurensis was 10.16%, whereas those of its solvent fractions (hexane, chloroform, ethyl acetate, butyl alcohol, and water) were 5.45, 11.39, 13.88, 26.07, and 40.80%, respectively. The total polyphenol contents and electron donating ability of 70% methanol extracts from stem bark of Maackia amurensis were 15.44 mg/g and 194.15 μg/mL of its IC50, respectively. The 70% methanol extracts showed the highest antioxidant activity. The total polyphenol content and total flavonoid content of chloroform fractions were higher in each of 201.98 mg/g and 13.55 mg/g. The chloroform fraction showed the lowest levels of DPPH(IC50, 183.95 μg/mL) and ABTS scavenging activity(IC50, 10.0 μg/mL). The antioxidant activity was detected in methanol extract, chloroform fractions. Conclusions : These results indicate that 70% methanol extract and its fractions of stem bark of Maackia amurensis, especially chloroform fraction, have the properties of anti-oxidant suggesting stem bark of Maackia amurensis may be a candidate for natural and functional materials.

S. cerevisiae를 이용한 발효 산마늘의 항당뇨활성

도은수(Doh Eun Soo),장준복(Chang Jun Pok),길기정(Kil Gi Jung ),정윤혜(Jeong Yun Hae),이건희(Lee Kun Hee),강기현(Kang Ki Hyun),지윤선(Ji Yoon Sun),김보람(Kim Bo Ram),김화랑(Kim Hwa Rang) 한국약용작물학회 2012 한국약용작물학회 학술대회논문집 Vol.2012 No.2

증숙 황정 물 추출물의 항산화 활성

강미원 ( Mi-won Kang ),장준복 ( Jun-pok Chang ),도은수 ( Eun-soo Doh ),길기정 ( Ki-jung Kil ),유지현 ( Ji-hyun Yoo ) 대한본초학회 2017 大韓本草學會誌 Vol.32 No.1

Objectives : The objective of this study is to evaluate antioxidant activity of water extracts from steamed Polygonati Rhizoma(PR). Methods : The alcohol steamed PR from Jinju-si, Gyeongsangnam-do and Samcheok-si Gangwon-do are treated for the research into the antioxidant activity of the water extract. The sample was divided into 4 groups that PR without steaming process, PR with once, second and third steaming process. The antioxidant activities of polyphenol, flavonoid, DPPH, SOD, hydroxyl radical and nitrite scavenging activity. Results : The total content of polyphenol was the grow rapidly when the number of steaming adds up. For the third time, both the steamed PR from Jinju-si and the one from Samcheok-si in the water extract indicate the highest content levels measured 50.59 ㎎/㎖ and 37.33 ㎎/㎖ respectively. The more the number of steaming increases, the more rising the content of flavonoid. The steamed PR from Jinju-si and Samcheok-si in the third time are measured 30.03 ㎎/㎖ and 54.9 ㎎/㎖ respectively. DPPH radical scavenging activity of the steamed PR rises high rather than the non-steamed one when the number of steaming increases. As increase steaming time, SOD like activity and hydroxyl radical scavenging ability were increasing too. The steamed PR from Jinju-si in the third time is measured as the highest level 22.4%, 71.90% respectively. As increase steaming time, nitrite scavenging ability was the number was decreased. Conclusions : These results suggest that water extracts from steamed PR , exhibited higher antioxidant activities can be potentially used as proper natural antioxidants.

택칠에서 분리한 Corilagin이 Collagen 유발 관절염에 미치는 영향 (Ⅰ) : - Corilagin을 투여한 류마티스 관절염 유발 생쥐의 형광유세포 분석 -

신삼기(Sam Kee Shin),장준복(Jun Pok Chang),도은수(Eun Soo Doh) 한국자원식물학회 2008 한국자원식물학회지 Vol.21 No.4

This study was intended to research into the possibility of corilagin (CRN) isolated from Euphorbia helioscopia as rheumatoid arthritis drug. For the study, CRN was medicated to the abdominal cavity of collagen induced arthritis (CIA) mice that was a animal model for rheumatoid arthritis and its effects on incidence and arthritis index (AI) were studied. The results were as folllows; Medicating corilagin that were isolated from Euphorbia heliscopia to CIA mice resulted in inhibiting an incidence of arthritis and reducing in arthritis index. It was found that inflammatory cells were remarkably decreased and joint cavity was well secured. Also it was displayed that the formation of pannus was not observed and a cartilage was preserved well by cell histological research. Performing fluorescence flow cytometry of CIA mice resulted in reducing in inflammatory cells infiltrated each tissue.

폐지를 이용한 생분해성 고분자의 제조 ( 1 ) - 폐지의 화학적 조성 분석 및 전처리 -

권기훈(Ki Hun Kwon),임부국(Bu Kug Lim),양재경(Jae Kyung Yang),장준복(Jun Pok Chang),이종윤(Jong Yoon Lee) 한국목재공학회 2000 목재공학 Vol.28 No.3

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지유 발효추출물의 항세균 및 항산화 활성

길기정 ( Ki-jung Kil ),도은수 ( Eun-soo Doh ),장준복 ( Jun-pok Chang ),이승현 ( Seung-hyun Lee ),유지현 ( Ji-hyun Yoo ) 대한본초학회 2015 大韓本草學會誌 Vol.30 No.4

Objectives: The objective of this research was to investigate the antibacterial and antioxidant activities of bio-fermented Sanguisorbae Radix extract. Methods: The Sanguisorbae Radix extract was fermented by Streptococcus thermophilus, and their products was tested for antibacterial activity against pathogenic microorganisms namely, Bacillus cereus, Bacillus subtilis, Staphylococcus aureus, Escherichia coli, Vibrio parahaemolyticus and Salmonella typhimurium by paper disc diffusion method and the antioxidant activities of extract was evaluated by five different assays as electron donating ability(EDA), superoxide dismutase(SOD)-like activity, polyphenol, flavonoid contents and nitrite scavenging ability. Results: The bio-fermented Sanguisorbae Radix extract was safe from heat. Antibacterial activity of fermented Sanguisorbae Radix extract appeared relatively highly against Bacillus cereus and Staphylococcus aureus and didn``t show any difference. EDA in comparison to Vitamin C showed over 90% activity at about the same time of Sanguisorbae Radix extract expressed highly. SOD activity showed 15% in fermentation before and after. The nitrite scavenging ability of Sanguisorbae Radix extract before and after fermentation showed higher numerical value over 70% in pH 2.5 than that of butylated hydroxytoluene(BHT). But SOD activity, EDA and nitrite scavenging ability were not different between the Sanguisorbae Radix extract before and after fermentation. Total polyphenol content expressed over about 20 mg/g, and that of the Sanguisorbae Radix extracts was increased than that of the fermented Sanguisorbae Radix extracts. Conclusions: The results suggest the usefulness of developing functional materials using antioxidant active Sanguisorbae Radix extract was fermented by Salmonella typhimurium with high polyphenol contents and nitrite scavenging ability.

산마늘 자생지의 임분구조와 식물종의 연관

김혜진 ( Hye Jin Kim ),윤총원 ( Chung Weon Yun ),도은수 ( Eun Soo Doh ),장준복 ( Jun Pok Chang ),최명석 ( Myung Suk Choi ),양재경 ( Jae Kyung Yang ),박희권 ( Hee Kwon Park ),신학섭 ( Hak Sub Shin ),이서희 ( Seo Hui Lee ),배관호 ( 한국산림과학회 2011 한국산림과학회지 Vol.100 No.4

This study was conducted to describe forest stand characteristics of the habitats of Allium victorialis var. platyphyllum population from the ecological analysis of classification of vegetation types, importance values, species diversity, interspecific association and community similarity in Ulleung Island and Mt. Hambaek, in which investigated by 48 plots (10 m×10 m). The vegetation studied was classified into 1 community group, 2communities, 2 groups and 3 subgroups for a species unit and divided by 5 units for a vegetation unit. In the Fagus engleriana group (vegetation unit 1, 2 and 3), importance value of A. victorialis var. platyphyllum in herb layer showed relatively higher value than other units. Importance value of A. victorialis var. platyphyllum in herb layer had higher value. In the analysis of interspecific association, most of the species associated positively to A. victorialis var. platyphyllum belonged to habitat features of valley area and sub-alpine zone. Therefore, it was considered that the growing environment of A. victorialis var. platyphyllum population was closely correlated to soil moisture and air humidity.