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장우종,장예은,조우현,이세현,심현아,박지영,Jiang Xu,Xiaofeng Shen,Baosheng Liao,조익현,김영창,양태진 한국육종학회 2022 Plant Breeding and Biotechnology Vol.10 No.3
Cultivation of the medicinal herb Panax ginseng Meyer began by domesticating wild mountain ginsengs severalhundred years ago in Korea. Elucidating the diversity of the maternally inherited plastid genome (plastome) in diverse ginsengcollections including wild ginsengs would provide valuable information on ginseng breeding and cultivation history. We sequencedand compared the plastomes of 44 ginseng accessions collected from various Northeast Asian countries. The plastomes revealed 18polymorphic sites, including 11 SNPs and 7 InDels, which portrayed less diversity than in the most closely related species, P. quinquefolius. We developed 10 kompetitive allele-specific PCR (KASP) markers and utilized them along with four previouslydeveloped InDel markers to characterize the genotypes of 203 ginseng accessions. Digital genotyping based on the developed KASPmarkers classified the accessions into 10 main and 2 branching haplotypes. Four InDel markers derived from different copy numbers oftandem repeats showed dynamic subgrouping within the haplotypes due to the occurrence of multi-alleles and reversible mutations. Thedigital haplotype genotyping (haplotyping) revealed that haplotype A, representing 60.1% of the accessions, might be the originalplastome form without any SNP occurrence. Accumulation patterns of the variations suggest that nine main haplotypes (B-J) divergedindependently by new SNP occurrences from the original plastome, and branching haplotypes may have derived from the first mutantlineage by additional SNP deposition. The digital haplotyping system based on plastome diversity deepens understanding of ginsengevolution and serves as a useful molecular breeding tool.
장우종,장예은,김남훈,Nomar Espinosa Waminal,Young-Chang Kim,Jung Woo Lee,Tae Jin Yang 고려인삼학회 2020 Journal of Ginseng Research Vol.44 No.4
Background: Ginseng (Panax ginseng Meyer) is one of the world’s most valuable medicinal plants withnumerous pharmacological effects. Ginseng has been cultivated from wild mountain ginseng collectionsfor a few hundred years. However, the genetic diversity of cultivated and wild ginseng populations is notfully understood. Methods: We developed 92 polymorphic microsatellite markers based on whole-genome sequence data. We selected five markers that represent clear allele diversity for each of their corresponding loci toelucidate genetic diversity. These markers were applied to 147 individual plants, including cultivars,breeding lines, and wild populations in Korea and neighboring countries. Results: Most of the 92 markers displayed multiple-band patterns, resulting from genome duplication,which causes confusion in interpretation of their target locus. The five high-resolution markers revealed3 to 8 alleles from each single locus. The proportion of heterozygosity (He) ranged from 0.027 to 0.190,with an average of 0.132, which is notably lower than that of previous studies. Polymorphism informationcontent of the markers ranged from 0.199 to 0.701, with an average of 0.454. There was nostatistically significant difference in genetic diversity between cultivated and wild ginseng groups, andthey showed intermingled positioning in the phylogenetic relationship. Conclusion: Ginseng has a relatively high level of genetic diversity, and cultivated and wild groups havesimilar levels of genetic diversity. Collectively, our data demonstrate that current breeding populationshave abundant genetic diversity for breeding of elite ginseng cultivars.
張宇種,朴鎭庸,한경,李珉湖,車京玉 明知大學校 産業技術硏究所 1997 産業技術硏究所論文集 Vol.16 No.-
This paper describes a theoretical and exprimental investigation of the pressure-time histories of some basic internal combustion engine exhaust systems. The program package is written in Fortran 77 and utilizes the Method of Characteristics to solve the general equations of one-dimensional unsteady gas flow. Using a rotary valve exhaust simulator, experimental pressure-time histories were obtained. The predictions are compared with measured results and show a high degree of correlation.
線形系와 2乘特性系의 結合 모델에 대한 스펙트럼 解析에 관한 硏究
李焌瑞,張宇種,金鳳珏,車京玉 明知大學校 産業技術硏究所 1998 産業技術硏究所論文集 Vol.17 No.-
This Paper describes a number of theoretical formulas to analyze the frequency domain properties of Gaussian input data passing through non-linear square-law systems. From measurement of input data and output data only, results are obtained to identify the separate frequency response functions for two models of linear systems in parallel with non-linear square-law systems. This formulas indicates also how to analyze other types of non-linear models by employing similar techniques.
이준서,장우종,차경옥 明知大學校 産業技術硏究所 1999 産業技術硏究所論文集 Vol.18 No.-
The pulsating pressure waves are composed of fundamental frequency and higher order harmonics in exhaust pipe of engines. The nonlinearity in exhaust pipe is caused by their interactions. The error which is between prediction and measurement is induced by the nonlinearity. We can not explain this phenomenon using linear acoustic theory which is existing theory. So power spectrum which was used in linear theory is not useful. Bispectrum and bicoherence functions which are a higher order spectrum are applicable to explain this phenomenon. This paper proposes a nonlinear effect of pulsating pressure waves. The phenomenon proposed here is identified by using of higher order spectrum density functions.