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잔나비걸상버섯 원형질체에 의한 만가닥버섯 염색체의 삽입
장권열,유영복,유창현 한국균학회 1988 韓國菌學會誌 Vol.16 No.4
The uptake of isolated Lyophyllum ulmarium chromosomes by Ganoderma applanatum protoplasts was induced with polyethylen glycol+CaCl₂+glycine. Uptake products of chromosomes by protoplasts showed micro-and macrotransgenome type. The former was slowly growing and unstable, the latter was outgrowing and stable mycelial colonies which made thick hyphae of width and segregation of mycelial colony on GCM containing benomyl. A comparison of macrotransgenome type was using isozyme analysis of esterase. The enzyme pattern of two transformants was distinct in position and quantity compared with parents.
장권열,유창현,고승주,변명옥,유형복 한국균학회 1987 韓國菌學會誌 Vol.15 No.1
This experiment was undertaken to investigate proper conditions for protoplast formation from Pleurotua apodoleucus. Novozym 234 was the moat effective enzyme and a high yield of protoplasta was obtained. Combination of enzymes did not improve this result. Sucrose gave the best result to support the release and maintain the stability of protoplasts. The optimal reaction time of mycelium with the lytic mixture was 3 hrs. in shaking condition at 120 strokes min^(-1). When the mycelium of P. spodoleucus was cultured for 3 days on mushroom complete agar medium at 27℃, the formation of protoplasta was effective. The mushroom complete agar medium stabilized with 0.6 M sucrose was the most effective for reversion of protoplasta.
박용환,장권열,유영복,유창현,이연희,존폐버디 한국균학회 1987 韓國菌學會誌 Vol.15 No.3
Interspecific heterokaryons were obtained by polyethylene glycol induced fusion of protoplaste from auxotrophic mutants of P. ostreatus+P. cornucopiae, P. ostreatus+P. florida, P. ostreatus+P. sajor-caju, P. florida+P. cornucopiae, P. florida+P. sajor-caju and P. sajor-caju+P. cornucopias protoplasts. The Fusion products of protoplasts were induced by MCM+benomyl, but segregation of sectors were not identified. Interspecific fusion products of protoplasts between incompatible strains did not form true clamp connections and did not produce fruit bodies. For induction of fertility, interspecific heterokaryons crossed with their parents by hyphal anastomosis.
박용환,장권열,유창현,유영부 한국균학회 1987 韓國菌學會誌 Vol.15 No.1
This experiment was undertaken to investigate proper conditions for protoplast formation from Lyophbllum ulmarium. Combination of Novozym 234, β-Glucuronidase and β-D-Glucanase with 0.6 M Sucrose was the most effective for isolation of protoplasts. The optimal reaction time of mycelium with the lytic mixture was 3 hrs in shaking condition at 120 strokes min^(-1). When the mycelium of L. ulmarium was cultured for 6 days on yeast glucose agar medium at 25℃, the formation of protoplasts was effective. The yeast glucose agar medium stabilized with 0.6 M sucrose was the most effective for reversion of protoplasts.
박용환,신평균,장권열,유영복,유창협 한국균학회 1987 韓國菌學會誌 Vol.15 No.4
The transfer of the isolated nuclei from P. Florida into protoplasts of P. ostreatus was induced with polyethylene glycol and CaCl₂. Three types of transfer products of nuclei were obtained when transferred to MMM. Type 1 colonies were more vigroualy growing mycelium and stable on MCM. One of the type 1 colonies, appeared segregation on MCM plus benomyl. The mycelium did not form clamp connection. These results suggest that type 1 colonies were nuclear hybrids or allodiploids. Type 2 was main products of nuclear transfer. The mycelium formed clamp connection and fertile on sawdust media. Type 3 was very slow growing or non-viable colonies after debris of nuclei or chromosomes transfer into recipient protoplasts. Isozyme pattern of esterase in type 1 produced a new band. Type 2 and type 3 could be characterized by parental bands.