볏짚을 이용한 사철느타리버섯의 배지 제조 및 종균재식에 관한 연구

차동열,박용환,고승주 한국균학회 1981 韓國菌學會誌 Vol.9 No.2

This study was performed to examine the cultivation of Pleurotus florida that forms pinhead between 5 and 25℃, on rice straw substrates in Korea. The highest yield of P. Florida sporophores was obtained from the fermented rice straw substrates added 5% rice bran to the rice straw by dry weight basis at the starting of fermentation. The pile turning of materials for uniform fermenting was made three times when the temperature of the pile reached 60℃ during out door composting. The 60℃ row steam was treated 6 hrs for pasteurization of the substrates in the cultivating house. The quantity of spawn affected the mycelial growth and the yield of P. florida. The optimum amount of spawn was 10% to the rice straw by dry weight basis mixed with the substrates. P. Florida produced normal sphorophores from September to middle of July of the following year.

양송이 마이코곤병의 방제약제에 관한 연구

차동열,김광포,전창성 한국균학회 1991 韓國菌學會誌 Vol.19 No.1

Prochloraz of fungicide was applied on Mycogone perniciosa causing wet bubble in cultivated mushroom, Agaricus bisporus. In vitro, Prochloraz was an excellent fungicide on two strains of Mycogone, tolerant and non-tolerant to Benomyl, respectively. At the low dosage, Prochloraz more inhibited mycelial growth of mushrooms than Benomyl. At the higher dosage, Benomyl more inhibited the mycelial growth than prochloraz. The higher yield of sporophore of the mushroom with low inferction rate was abtained from several trial of Prochloraz. Prochloroz was concluded to be effective fungicide on Mycogone perniciosa on Agaricus cultivation.

양송이 균사 생장과 자실체 수량에 미치는 몇가지 환경요인의 영향

차동열,신관철,박종성 한국균학회 1981 韓國菌學會誌 Vol.9 No.1

Attempts were made to investigate the effects of the temperature and carbon dioxide concentration in the growing room and soil moisture after casing on the mycelial growth, sporophore formation and mushroom yield of Agaricus bisporus (Lange) Sing. The growing room temperature influenced the mycelial growth in the casing layer after casing and the sporophore formation of Agaricus bisporus. The mycelial growth was the rapidest at 30℃ and gradually decreased with the temperature going down, while the sporophore formation and mushroom yield were the best at 25℃. The other factor which affected the mycelial growth and sporophore formation was the moisture content of casing soil. The mycelial growth was the best at 70 percent moisture, and the sporophore formation and mushroom yields were the highest at 60 percent moisture. The concentration of carbon dioxide in the growing room after casing had an important effect upon the mycelial growth in the casing layer and the sporophore formation. When the concentration of carbon dioxide was 0.16 percent, the mycelial growth and the sporophore formation were not inhibited. At 0.5 to 2.0 percent CO₂ the myceilal growth and the sporophore formation were severely decreased. The sporophore size of the mushroom was the maximum when the room temperature during the vegetative mycelium growth was 20℃ and the moisture content of casing soil was 70 percent.

느타리버섯과 사철느타리버섯의 균주간 및 종간 교배

차동열,박용환,고승주 한국균학회 1981 韓國菌學會誌 Vol.9 No.1

This study was performed to investigate the high yielding potential of Pleurotus ostreatus (Fr.) Quel derived from intermating and intramating of three strains: Local strain, Nonggi 201, and Pleurotus florida Eger. All the strains tested were tetrapola mating systems, and hybridable. Intermating system resulted in more fresh sporophores than those from intramating. The highest yield of fresh sporophores of P. ostreatus was obtained from Nonggi 201 crossed with P. florida, when cultivated on the rice straw substrates.

농산부산물(農産副産物)을 이용(利用)한 식용(食用)버섯 재배(栽培)

차동열,박정식,Cha, Dong-Yeul,Park, Jeong-Sik 한국응용생명화학회 1984 Applied Biological Chemistry (Appl Biol Chem) Vol.27 No.suppl.

양송이 재배(栽培)에 있에서 볏짚을 주재료(主材料)로 사용(使用)할때의 퇴비재료(堆肥材料)의 배합(配合), 야외퇴적(野外堆積) 및 후발효(後醱酵)에 관(關)한 시험(試驗)과 볏짚을 이용(利用)한 느타리버섯 재배(栽培)에 관(關)한 연구(硏究)를 수행(遂行)한 바 그 결과(結果)를 요약(要約)하변 다음과 같다. 가) 합성퇴비배지(合成堆肥培地)의 탄소원(炭素源)으로서 볏짚은 보리짚과 밀짚보다 발효(醱酵)가 신속(迅速)하고 퇴비(堆肥)의 전질소함량(全窒素含量)이 높으며, 배지(培地)의 질(質)이 양호(良好)하여 양송이 자실체수량(子實體收量)이 현저(顯著)히 높았다. 나) 보리짚퇴비(堆肥)는 볏짚퇴비(堆肥)보다 생산성(生産性)이 낮으나 보리짚과 볏짚을 50:50으로 혼합(混合)하면 볏짚과 대등(對等)한 수량(收量)을 얻을 수 있었다. 다) 퇴비배지(堆肥培地)의 전유기태질소(全有機態窒素)와 자실체수량간(子實體收量間)에는 정(正)의 상관(相關)이 있으나 암모니아태질소(態窒素)와는 균사생장(菌絲生長) 및 자실체수량(子實體收量)에 부(負)의 상관(相關)이 있었다. 라) 볏짚을 주재료(主材料)로 사용(使用)할때 무기태질소원(無機態窒素源)으로서 요소(尿素)가 가장 좋았고 유안(硫安)과 석회질소(石灰窒素)는 부적당(不適當)하였다. 요소(尿素)는 3회(回) 분시(分施)할때 손실(損失)이 감소(減少)되었고, 퇴비(堆肥)의 질소함량(窒素含量)이 증가(增加)하였다. 마) 유기태영양원중(有機態營養源中) 들깨묵, 참깨묵, 밀기울, 계분등(鷄糞等)의 첨가(添加)는 퇴비(堆肥)의 발효(醱酵)를 양호(良好)하게 하고 자실체수량(子實體收量)을 증가(增加)시켰다. 바) 양송이 볏짚퇴비(堆肥)를 제조(製造)할때 터널기계화(機械化)하므로 수량(收量)이 13 % 증수(增收)되었고,생산비(生産費)가 34 % 절감(節減)되었다. 사) 볏짚과 밀짚을 이용(利用)하여 느타리버섯 재배(栽培)가 가능(可能)하고 자실체수량(子實體收量)을 뱃짚구(區)에서 높았다. 아) 느타리버섯 볏짚배지(培地)를 $60^{\circ}C$로 6시간(時間) 열처리(熱處理)하므로서 균사생장(菌絲生長)이 양호(良好)하였고 자실체수량(子實體收量)이 높았다. The results of series studies on the ratio of supplements, out-door composting and out-door fermentation induced by using the rice straw as a main substrates at the cultivation of Agaricus bisporus, and the cultivation of Pleurotus ostreatus using the rice straw bundles on its compost are as follows; When rice straw for cultivation of A. bisporus was used as the main substrates in synthetic compost as a carbon source, yields were remarkably high. Fermentation was more rapid than that of barley straw or wheat straw and the total nitrogen content was high in rice straw compost. Use of barley straw compost for cultivation of A. bisporus was shown of low yield compared with rice straw, but when a 50% barley straw and 50% rice straw mixture was used, the yield was almost the same as that using only rice straw. The total organic nitrogen on the compost were shown the positive relation to the yield of A. bisporus, but the ammonium nitrogen negative relation to the mycelial growth and yield of A. bisporus. When rice straw was used as the main substrate for compost media, urea was the most suitable source of nitrogen. Poor results were obtained with calcium cyanamide and ammonium sulfate. When urea was applied three separate times, nitrogen loss during composting was decreased and the total nitrogen content of compost was increased. The supplementation of organic nutrient activated compost fermentation and increased yield of A. bisporus. The best sources of organic nutrients selected were as follows: perilla meal, sesame meal, wheat bran and poultry manure, etc. Soybean meal, tobacco powder and glutamic acid fermentation byproducts which were industrial wastes, could be substituted for perilla meal, sesame meal and wheat bran as organic nutrient sources for compost media. During out door composing of rice straw for cultivation of A. bisporus, using of tuner, composter and tunnel system increased up to 13% of its yield, and also cut down 34% of production Cost. The cultivation of P. ostreatus and utilizing of rice straw and wheat straw was established and its yield was high on the rice straw pots. When the substrates 'Rice straw' was heated by steam at $60^{\circ}C$ for 6 hr. mycelial growth of P. ostreatus was moderately rapid and its yield was high.

D-4. SEM 에 의한 담자포자 표면의 미세구조 관찰

Kim, Yang Sup,Cha, Dong Yeul,Chung, Hwan Chae,Park, Yeong Hwan 한국균학회 1985 韓國菌學會誌 Vol.13 No.4

양송이 괴균병균의 방제 및 생장요인에 관한 연구

정후섭,차동열,김광포 한국균학회 1981 韓國菌學會誌 Vol.9 No.1

Mycelial growth and fruit body formation of Diehlomyces microsporus were best on mushroom spawn extract medium and rice bran extract medium, respectively. L-asparagine, fructose and glucose were good nutrient sources for mycelial growth. Optimum temperature for mycelial growth ranged at 25∼28℃ Maximum mycelial growth occurred at pH 5.5 while optimum pH for ascospore germination was 6.0. Mycelial mats of D. microsporus did not survive at 60℃ for 60 minutes while ascospores at 80℃ for 120 minutes. Damages of fruit body of Agaricus bisporus caused by D. microsporus were maximum when the fruit bodies were infected at spawning and casing on the compost. The truffle disease could be controlled by basamid with 100∼150ppm treating on the compost after filling.

4. 여름느타리버섯 (Pleurotus sajor - caju) 과 느타리버섯 (Pleurotus ostreatus) 의 균사생장에 영향을 미치는 몇가지 요인에 관한시험

박용환,차동열,고승주,유창현 한국균학회 1983 韓國菌學會誌 Vol.11 No.4

The experiment was carried out to investigate some factors affecting mycelial growth of Pleurotus sajorcaju and P. ostreatus. The optimal temperature for mycelial growth of both the species were from 25 to 30℃ whereas the formation of primordia of the sporophores was 20 to 25℃ in P. sajor-caju and 15℃ in P. ostreatus respectively on potato sucrose yeast agar medium. The optimal pH of the medium for the mycelial growth of both the species were ranged 5 to 6. The effect of carbon and nitrogen sources on mycelial growth of the species were studied using modified Waksman's special medium as the basal medium with the test substance. Nine carbon and eight nitrogen sources were evaluated by replacing the carbohydrates or nitrogen in the basal medium. Generally, disaccharides and polysaccharides showed good effect for the mycelial growth of P. sajor-caju, but polysaccharides were superior to the other classes of carbon sources for the mycelial growth of P. ostreatus. Generally, organic nitrogen compounds showed good mycelial growth of both the species. The mycelial growth of the species were favored by ammonium more than by nitrate nitrogen source. Asparagine was superior to the other kinds of amino acid. Six percentages of glucose and 0.6% of ammonium tartrate was optimum for mycelial growth of P. sajor-caju whereas the optimum concentration for the P. ostreatus was 4% of glucose and 0.4% ammonium tartrate.

느타리버섯과 사철느타리버섯의 원형질체 재생 및 환원에 관한 연구

유영복,차동열,죤페버디 한국균학회 1985 韓國菌學會誌 Vol.13 No.2

The experiment of protoplast regeneration and reversion were undertaken to provide a basic techniques for protoplast manipulation. Protoplasts of Pleurotus florida and Pleurotus ostreatus were reverted to normal hyphal growth and the reversion frequency of both fungal protoplasts were 0.24∼3.19%. Reversion medium stabilized with 0.6 M potassium chloride and sucrose was better than the other stabilized one. The protoplast reversion frequency was increased when various amino acid and vitamin compounds were added to the hypertonic mushroom complete agar medium.

E-4. 잎새버섯의 배양적 특성과 자실체 형성에 관한 연구

김양섭,차동열,박용환,정환채 한국균학회 1985 韓國菌學會誌 Vol.13 No.4