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박용환,장권열,유창현,유영부 한국균학회 1987 韓國菌學會誌 Vol.15 No.1
This experiment was undertaken to investigate proper conditions for protoplast formation from Lyophbllum ulmarium. Combination of Novozym 234, β-Glucuronidase and β-D-Glucanase with 0.6 M Sucrose was the most effective for isolation of protoplasts. The optimal reaction time of mycelium with the lytic mixture was 3 hrs in shaking condition at 120 strokes min^(-1). When the mycelium of L. ulmarium was cultured for 6 days on yeast glucose agar medium at 25℃, the formation of protoplasts was effective. The yeast glucose agar medium stabilized with 0.6 M sucrose was the most effective for reversion of protoplasts.