Tacotron2 기반 한국어 음성 합성 모델 개발과 한국어에 맞는 Hyper-parameter 탐색

임주원(Joo-Won Lim),배우근(Woo-Geun Bae),김휘수(Hwi-Soo Kim),김영수(Young-Soo Kim),이명우(Myong-Woo Lee),황영태(Yeong-Tae Hwang) 한국정보과학회 2018 한국정보과학회 학술발표논문집 Vol.2018 No.12

Studies on the Peroxidase Activity of Rabbit Hemoglobin

주한승,임주원,김승수,Joo, Han-Seung,Lim, Joo-Won,Kim, Soung-Soo 생화학분자생물학회 1991 한국생화학회지 Vol.24 No.6

토끼 erythrocyte lysate으로부터 hemoglobin을 $(NH_4)_2SO_4$ 분별침전, CM-Sephadex 크로마토그래피, hydroxyapatite 크로마토그래피, Sephadex G-l00 겔 크로마토그래피 방법을 이용하여 순수 정제하였다. 정제한 토끼 hemoglobin은 peroxidase 활성을 나타냈으며 guaiacol과 o-dianisidine을 기질로 사용하였을 경우 식물 peroxidase보다 10배 가량 낮은$K_m$값을 나타냈다. 여러 phenol 화합물들을 기질로 사용하였을 때 토끼 hemoglobin은 caffeic acid, chlorogenic acid, indole-3-acetic acid를 빠르게 산화하였으며, disubstituted phenol 화합물과 methoxylated phenol 화합물들이 rabbit hemoglobin에 대하여 친화력이 큰 것으로 나타났다. Chemical modification 실험결과 토끼 hemoglobin은 iodoacetamide와 N-ethylmaleimide에 의하여 활성이 억제되지 않지만 p-chloromercuribenzoate에 의해서는 활성이 현저히 억제되었다. 또한 식물 peroxidase와는 달리 hemoglobin의 peroxidase 활성은 diethylpyrocarbonate에 의하여 급격히 불활성화 되었다. Rabbit hemoglobin was purified by using ammonium sulfate fractionation, CM-Sephadex chromatography, hydroxyapatite chromatography and Sephadex G-100 gel filtration. The purified hemoglobin oxidized various synthetic and naturally occurring phenolic compounds in the presence of $H_2O_2$. The substrate specificity studies indicate that rabbit hemoglobin has about 10 times lower $K_m$ values for guaiacol (0.39 mM) and o-dianisidine (0.09 mM) when compared to those of plant peroxidases. Among natural substrates tested, rabbit hemoglobin catalyzes the oxidation of caffeic acid, chlorogenic acid and indole-3-acetic acid quite rapidly compare to other naturally occurring phenolic compounds such as scopoletin, esculetin, ferulic acid and p-coumaric acid. In general, phenolic compounds having methoxy group appears to have high affinity to rabbit hemoglobin as revealed by their very low $K_m$ values. Chemical modification studies indicate that p-chloromercuribenzoate inactivates the peroxidase activity of rabbit hemoglobin, whereas N-ethylmaleimide or iodoacetamide does not. Furthermore, the peroxidase activity of rabbit hemoglobin is rapidly inactivated by diethylpyrocarbonate, a histidyl selective reagent, unlike plant peroxidases.

von Hippel-Lindau병: 1예 보고

고경란,임주원,이범하,이동호,고영태,Ko, Kyung-Ran,Lim, Joo-Won,Lee, Bum-Ha,Lee, Dong-Ho,Ko, Young-Tai 대한영상의학회 2002 대한영상의학회지 Vol.46 No.1

von Hippel-Lindau병은 상염색체 우성으로 유전하는 질환으로 망막 혈관모세포종, 중추신경계의 혈관모세포종, 췌장 낭종과 종양, 신세포암, 크롬친화세포종, 부고환 낭선종 등을 초래한다. 저자들은 가족성을 보이는 von Hippel-Lindau병 1예를 경험하였기에 문헌 고찰과 함께 방사선학적 소견을 보고하고자 한다. Von Hippel-Lindau disease is an autosomal dominant disorder characterized by the presence of multiple benign and malignant tumors including hemangioblastomas of the retina and central nervous system, pancreatic cysts and tumors, renal cell carcinomas, pheochromocytomas and epididymal cystadenomas. We report the radiologic findings of a case of von Hippel-Lindau disease, describing the family history.

토끼 Hemoglobin 의 Peroxidase 활성에 관한 연구

주한승,임주원,김승수 ( Han Seung Joo,Joo Won Lim,Soung Soo Kim ) 생화학분자생물학회 1991 BMB Reports Vol.24 No.6

Rabbit hemoglobin was purified by using ammonium sulfate fractionation, CM-Sephadex chromatography, hydroxyapatite chromatography and Sephadex G-100 gel filtration. The purified hemoglobin oxidized various synthetic and naturally occurring phenolic compounds in the presence of H₂O₂. The substrate specificity studies indicate that rabbit hemoglobin has about 10 times lower K_m values for guaiacol (0.39 mM) and o-dianisidine (0.09 mM) when compared to those of plant peroxidases. Among natural substrates tested, rabbit hemoglobin catalyzes the oxidation of caffeic acid, chlorogenic acid and indole-3-acetic acid quite rapidly compare to other naturally occurring phenolic compounds such as scopoletin, esculetin, ferulic acid and p-coumaric acid. In general, phenolic compounds having methoxy group appears to have high affinity to rabbit hemoglobin as revealed by their very low K_m values. Chemical modification studies indicate that p-chloromercuribenzoate inactivates the peroxidase activity of rabbit hemoglobin, whereas N-ethylmaleimide or iodoacetamide does not. Furthermore, the peroxidase activity of rabbit hemoglobin is rapidly inactivated by diethylpyrocarbonate, a histidyl selective reagent, unlike plant peroxidases.

전산화 단층촬영을 이용한 한국인 간경화증 환자의 미상엽-우엽비

양익,임주원,이동호,고영태,임재훈,Yang, Ik,Lim, Joo-Won,Lee, Dong-Ho,Ko, Young-Tae,Lim, Jae-Hoon 대한영상의학회 1994 대한영상의학회지 Vol.30 No.6

췌장의 고형 유두상 상피종의 방사선학적 소견 : 병리 소견과 비교

양익,임주원,고영태,임재훈,양달모,김은경,곽정호,Yang, Ik,Lim, Joo-Won,Ko, Young-Tae,Lim, Jae-Hoon,Yang, Dal-Mo,Kim, Eun-Kyung,Kwak, Jeong-Ho 대한영상의학회 1994 대한영상의학회지 Vol.30 No.1

목 적: 췌장의 고형유두상상피종에 대한 CT 및 초음파의 전형적인 소견들이 여러 문헌에 보고 되었으나 저자들의 경험한 바에 따르면 이들 전형적인 소견 이외에도 비전형적인 소견들이 있어 육안적 조직소견과 함께 비교하여 보고한다. 대상 및 방법: 1980년부터 1992년까지 12년간 수술로 확진된 췌장의 고형유두상상피종 14예를 대상으로 하였는데 남녀비는 1:13이었고 연령별로는 15세부터 49세까지로 평균 연령은 27세이며 CT에 13예, 초음파는 12예에서 시행하였다. 방법으로는 CT와 초음파에서 종괴의 위치, 크기, 밀도, 석회화, 중격등을 후향적으로 분석하여 수술 소견 및 육안적 병리조직과의 상호 관련성을 비교하였다. 결 과: 종괴의 위치는 두부 6예(43%), 미부 5예(36%), 체부 1예(7%) 그 외 두-체부,체-미부 각각 1예(7%)로 비교적 \ulcorner\ulcorner은 나이의 (평균 연령 27세) 여자(93%)였다. 전형적인 소견은 CT 및 초음파에서 주위 조직과 경계가 분명한 종괴로 고형성 종괴는 비교적 피막으로 잘 싸여진 과립상, 유두상 조직으로 보였고 낭성 종괴는 초코렛 색의 진흑같은 출혈성 괴사 조직이었다. 비전형적인 소견으로 종괴내의 석회화는 6예(43%), 중격은 4예(31%)볼 수 있었다. 석회화의 양상은 종괴의 주변부, 내부등에서 다양한 소견으로 보였다. 결 론 : 췌장의 고형유두상상피종의 전형적인 방사선학적, 병리학적 소견 외 종괴의 석회화, 중격등을 육안 병리 소견으로 확인하였는데 이런 비전형적인 방사선학적 소견의 이해는 췌장의 고형유두상상피종의 정확한 수술전 진단을 하는데 도움이 될 것이다. Purpose : Computed tomographic(CT). ultrasonographic(US) findings of soild and papillary epithelial neoplasm of the pancreas were correlated with pathologic findings for the better understanding of this disease entity. Materials and Methods : A retrospective review of CT and US of 14 cases of solid and papillary epithelial neoplasm of the pancreas was carried out in terms of the margin, internal architecture, calcification and septation, and this was correlated with gross pathologic findings.Results : CT and US findings were well defined round masses consisting of both solid and cystic components. Five cases were cystic, four cases were solid and five cases were mixed. Cystic portion of the tumor represented variable degree of hemorrhagic necrosis. Six cases contained foci of calcification , which were linear, marginal and amorphous. Marginal calcification interfered US examination of the mass in three cases. Internal septum was demonstrated in four cases on CT, one case on US and three cases on gross specimen.Conclusions : Our results indicate that calcification and internal septum were considered as a part of radiologic findings in solid and papillary epithelial neoplasm of the pancreas.

가토 간내 선택적 담관결찰후 간부피의 변화에 대한 실험적 연구

이혜원,윤엽,고영태,최우석,임주원,오주형,임형택,김윤화,이석환,Lee, Hye Won,Yoon, Yup,Ko, Young Tae,Choi, Woo Suk,Lim, Joo Won,Oh, Joo Hyeong,Rim, Heyong Teck,Kim, Youn Wha,Lee, Seok Hwan 대한영상의학회 1998 대한영상의학회지 Vol.39 No.6

Purpose : To evaluate the role of bile duct obstuction in the development of atrophy of the liver, using ananimal model. Materials and Methods : Seven rabbits were divided into two groups : group 1(n=5), in which therewas selective bile duct ligation, and group 2(n=2), which underwent a sham operation. Each group was evaluatedusing CT for changes in hepatic volume after selective bile duct ligation or a sham operation. In group I, thediameter of dilated bile duct was measured 2, 4, 8, 12 and 16 weeks after bile duct ligation, while gross andhistologic change were evaluated in all cases. Results : In group 1, bile duct dilatation was seen on CT two weeksafter selective bile duct ligation, and did not change significantly during follow-up. In four of five cases, CTrevealed no evidence of significant atrophy of the involved segment. Pathologic specimens, however, revealeddilatation of the bile duct, periductal fibrosis, infiltration of chronic inflammatory cells, and periportalfibrosis. One of five cases showed segmental liver atrophy after selective bile duct ligation. In addion to theabove pathologic findings, there was obstruction of the portal vein by foreign body reaction. In group 2, noevidence of dilated bile duct or liver atrophy was revealed by CT or pathologic specimen after a sham operation.Conclusion : During long-term follow-up of 16 weeks, obstruction of the bile duct did not play a major role in thedevelopment of lobar atrophy in the rabbit.

매독성 위염 : 증례보고

양익,이동호,고영태,임재훈,임주원,Yang, Ik,Lee, Dong-Ho,Ko, Young-Tae,Lim, Jae-Hoon,Lim, Joo-Won 대한영상의학회 1992 대한영상의학회지 Vol.28 No.4

Syphilitic involvement of the stomach often takes the appearance of neoplastic conditions. We describe the radiological findings of syphilitic gastritis. A 36-year-old man was diagnosed with gastric cancer by upper GI and CT, but syphilitic gastritis was confirmed by endoscopy and upper GI series performed after penicillin therapy. We report a case of syphilitic gastritis presented as advanced stomach cancer by clinical, endoscopic and radiological study.

도세포종의 방사선학적 진단 : 경피 경간 문정맥 , 췌정맥 삽관술 및 홀몬 분석

양익,윤엽,고영태,임주원,임재훈,Yang, Ik,Yoon, Yup,Ko, Young-Tae,Lim, Joo-Won,Lim, Jae-Hoon 대한영상의학회 1993 대한영상의학회지 Vol.29 No.4

Two patients with clinical and biochemical evidences of islet cell hyperfunction underwent percutaneous transhepatic portal and pancreatic vein catheterization and hormone assays. Local step-ups of insulin level in the protal venous system, found in two patients with insulinoma, corresponded to tumor sites at surgery. One patient had single insulinoma and the other had malignant insulinoma and liver metastasis. Percutaneous transhepatic protal and pancreatic vein catheterization with measurement of radioimmunoactive insulin concentration is a safe and reliable method, and may play an important role in the localization of adenoma in patients with hyperinsulinism.

초음파를 이용한 전립선의 경직장 자동총생검

양익,임재훈,고영태,이동호,임주원,Yang, Ik,Lim, Jae-Hoon,Ko, Young-Tae,Lee, Dong-Ho,Lim, Joo-Won 대한영상의학회 1994 대한영상의학회지 Vol.30 No.1

목 적: 경직장 초음파를 이용한 전립선의 자동총생검의 효용성과 임상적 유용성을 알아보기위해 저자들은 자동총생검후의 병리 조직학적 진단과 환자의 상태를 비교 분석하였다. 대상 및 방법: 전립선 질환을 갖고있는 24명의 입원 환자를 대상으로 전립선 생검은 시술전 마취없이 환자를 좌측와위로 눕히고 18게이지 생검 바늘을 장착한 자동총생검을 사용해 경직장 초음파 유도하에 시행하였는데, 초기 22예는 2-4군데서, 최근 2예는 여섯군데서 생검을 시행하였다. 결 과: 생검 조직은 21예에서 병리 조직학적 진단을 내리기에 충분하였으나 3예에서는 불충분하였다 모든환자는 경증의 통증을 호소하였으나 임상적으로 중요시될 합병증은 한 예도 없었다. 결 과: .전립선 질환의 진단에 있어서 경직장 초음파를 이용한 전립선의 자동총생검은 비교적 쉽고 시술하기가 간편한 매우 유용한 진단방법이 될 수 있을 것이다. Purpose : To assess the effectiveness and clinical usefulness of prostate biopsy by automated gun biopsy device under the transrectal ultrasonographic guidance, authors analysed the result of biopsy and the patients status after biopsy procedure.Materials and Methods : The subjects consisted of 24 patients with prostatic disease. Biopsy instrument was an automated gun biopsy device loaded with an 18 gauze biopsy needle. All the patients were admitted to the hospital. No analgesics was given. All the procedure was performed with the patient in left lateral decubitus. Biopsy was performed at 2-4 different points of the prostate in 22 cases, but recently, six different points were targeted in two patients.Results : Biopsy specimens were sufficient in 21 cases but insufficient in three cases. Histologic examination of biopsy specimens showed that 13 cases were nodular hyperplasia, eight cases were cancerous and three cases were inflammation. There was no clinically significant complication. There was mild to moderate degree of pain in all patients.Conclusion : Tansrectal biopsy of the prostate with an automated gun biopsy device under ultrasonographic guidance is considered relatively easy, handy and useful procedure in patients with prostatic disease. The procedure may be performed on the outpatient basis.