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중합효소연쇄반응을 이용한 돼지 증식성 장염 진단기법 확립
임숙경,이희수,우승룡,윤순식,문운경,이유영,고홍범,Lym, Suk-kyung,Lee, Hee-soo,Woo, Sung-ryong,Yoon, Soon-seek,Moon, Oun-kyong,Lee, Yoo-young,Koh, Hong-bum 대한수의학회 1999 大韓獸醫學會誌 Vol.39 No.1
Porcine Proliferative Enteropathy(PPE) is an infectious enteric disease and a major cause of economic loss in swine industry due to weight loss, poor growth and sudden death in growing and finishing pigs at 6 to 20 weeks of age. PPE has been diagnosed by clinical signs, syndrom and lesions in the intestine in Korea. However, the diagnostic method had several problems in the detection of infected or carrier pigs. Therefore, in this study, we established the polymerase chain reaction(PCR) which was a fast, specific and sensitive method for identification of Lawsonia intracellularis (L intracellularis). We designed and synthesized primer on the 16S rDNA and p78 gene encoding L intracellularis. Specificity of the method was confirmed by comparison of the PCR results using other enteric bacteria and the study has shown that PCR method was sensitive to detect 1ng of genomic DNA as a template. Identity of the PCR products was confirmed by comparison of pattern of restriction endonuclease analysis with restriction enzyme Hae III and Pst I. Also, the PCR method was applicable to the naturally affected pigs with PPE. Based on the results from this study, the PCR method could be used as a fast and specific diagnostic tool for PPE.
젖소 유방염 유래 Staphylococcus aureus의 Coagulase Gene 유전형 분석에 의한 감염경로 규명
문진산,이애리,임숙경,주이석,강현미,김종만,김말남,Moon, Jin-San,Lee, Ae-Ri,Lym, Suk-Kyung,Joo, Yi-Seok,Kang, Hyun-Mi,Kim, Jong-Man,Kim, Mal-Nam 대한수의학회 2003 大韓獸醫學會誌 Vol.43 No.1
Because Staphylococcus aureus (S. aureus) has variable number of short sequence repeat region in coagulase gene, it has been used to investigate the relatedness of S. aureus isolates. In this study, we isolated S. aureus strains from 20 dairy farms with bovine mastitis from September 2000 to August 2001. PCR-RFLP analysis of coagulase gene revealed 10 different patterns. Most of the S. aureus isolates showed only one coagulase gene RFLP pattern per farm. However, there were several S. aureus clones spreading between dairy farms. All the farms showed poor management conditions of milking machine and milker, indicating that managements for mastitis control program include use of proper milking matching, premilking sanitation, and segregation in the S. aureus infection herd. Our data suggest that PCR-RFLP analysis of coagulase gene might be applicable for the epidemiological investigations of S. aureus isolated from bovine mastitis cows.