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클로람페니콜 내성 플라스미드 pKH7의 Rep 단백질과 CAT 단백질의 염기서열 분석
윤성준(Sung Joon Yoon),이대운(Dae Woon Lee),김우구(Woo Koo Kim),신철교(Chul Kyo Shin),임성환(Sung Hwan Im),문경호(Kyung Ho Moon) 대한약학회 1995 약학회지 Vol.39 No.6
The nucleotide sequence of XbaI-MboI fragment of pKH7, a chloramphenicol-resistant(Cmr) plasmid isolated from multidrug-resistant S. aureus SA2, has been determined. XbaI-MboI fragment of pKH7 was found to contain two ORFs. One ORF encoded Rep and the other encoded CAT protein. The deduced amino acid sequences of Rep and CAT of pKH7 were compared to those of pUB112 and pC221. Comparisons revealed that there was one amino acid dfference in CAT between pKH7 and pUB112. CAT of pKH7 exhibited 98.6% amino acid identity to that of pC221. In case of Rep proteins, a slightly lower homology of 96.4% and 86.7% in amino acid sequences was observed betweeen pKH7 and pUB112 and between pKH7 and pC221, respectively.