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정동근,Changrok Choi,Sung Il Kim,,인경민,박헌용 한국물리학회 2008 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.53 No.3
Cells attached on surfaces can be used for many biological applications, such as cell chips. For some purposes, surfaces with excellent cell attachment and subsequent growth are needed. In this work, plasma-polymerized ethylenediamine (PPEDA) thin films were deposited on glass slides by using a plasma-enhanced chemical-vapor deposition system with an ethylenediamine precursor. In previous reports, PPEDA thin films were shown to have a high density of amine functional groups on their surfaces. Bovine aortic endothelial cells (BAECs) tested in this study were grown on a PPEDA film in Dulbecco's modified Eagle's medium containing 20 % Fetal Bovine Serum at 37 ℃ and 5 % CO2. The BAECs were revealed to have adhered to the surface of the PPEDA film and to have grown as on commercial culture dishes. By estimating the cell growth rates, we found the PPEDA film to be better than commercial culture dishes. The PPEDA film can be applied for a variety of biological assay techniques.
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Chunghyul-dan acts as an anti-inflammatory agent in endothelial cells by regulating gene expression
정우상,박헌용,조진구,조기호,박정미,문상관,김경욱,박성욱,피재호,박상규,정윤화,인경민,고창남,김종민 한국통합생물학회 2010 Animal cells and systems Vol.14 No.4
Chunghyul-dan (CHD) is a combinatorial drug known to exert anti-inflammatory effects in endothelial cells. In this study, we employed global transcriptional profiling using cDNA microarrays to identify molecular mechanisms responsible for the anti-inflammatory activity of CHD in endothelial cells. An analysis of the microarray data revealed that transcript levels of monocyte chemotactic protein-1 (MCP-1), vascular cell-adhesion molecule-1(VCAM-1) and activated leukocyte cell-adhesion molecule were dramatically altered in CHD-treated endothelial cells. These changes in gene expression were confirmed by RT-PCR, Western blotting and ELISA. Chronic CHD treatment also appeared to decrease MCP-1 secretion, probably as a result of decreased MCP-1 expression. In addition, we determined that chronic CHD treatment inhibited lipopolysaccharide-stimulated adhesion of THP-1leukocytes to endothelial cells. The inhibitory effect of CHD on LPS-stimulated adhesion resulted from downregulation of VCAM-1 expression. Transmigration of THP-1 leukocytes through endothelial cells was also inhibited by chronic CHD treatment. In conclusion, CHD controls a variety of inflammatory activities by regulating MCP-1and VCAM-1 gene expression.
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최승민(Seungmin Choi),박종봉(Jongbong Park),김종민(Jongmin Kim),인경민(Kyungmin In),박헌용(Heonyong Park) 한국생명과학회 2008 생명과학회지 Vol.18 No.5
생약 재료로 사용되어 왔던 가시오가피 추출액의 혈관 생리 활성을 평가하기 위하여 혈관 내피세포에서 다양한 실험을 수행하였다. 가시오가피 추출액은 혈관 내피세포 성장을 촉진하면서 세포사멸에는 영향을 미치지 않았다. 또한 혈관 내피세포의 세포 이동이 가시오가피 추출액에 의하여 촉진됨을 알 수 있었다. 이와 같은 연구 결과를 보면, 가시오가피 추출액이 혈관 형성을 유발시키는 작용을 하는 것으로 예측된다. 또한 가시오가피 추출액이 산화질소(NO)의 생산을 증가시키는 기능과 THP-1의 내피세포 부착을 억제하는 기능을 동시에 갖고 있음을 확인하였다. 이러한 결과는 가시오가피가 동맥경화 발생을 억제시키는 약물임을 의미한다. 또한 가시오가피 추출액은 THP-1 세포의 동종세포 응집을 촉발하는 기능을 함으로서 상처 난 부위에서 일어나는 출혈을 억제할 것으로 예측된다. 결론적으로 가시오가피 추출액은 다양한 혈관 생리 활성을 갖는 생약 재료로 평가된다. Crude extracts of Acanthopanax senticosus (A. senticosus) have been used as galenicals. In this study we carried out a series of experiments to examine cardiovascular activities of crude extracts from A. senticosus. Whereas the extracts had no effect on the apoptosis of bovine aortic endothelial cells (BAECs), the extracts appeared to induce endothelial cell proliferation. In addition, endothelial cell migration was shown to be enhanced by the crude extracts, indicating that A. senticosus extracts likely act as an angiogenic factor. We also examined effects of A. senticosus extracts on nitric oxide (NO) production, leukocytic adhesion to endothelium, and homotypic aggregation of leukocytes. Both the NO production and the leukocytic adhesion to endothelial cells were revealed to be increased by treatment with A. senticosus extracts, but the homotypic aggregation of leukocytes was inhibited. Together, multiple roles of A. senticosus extracts in the cardiovascular system provide an insight on their broad applications as galenicals to treat cardiovascular diseases.
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