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효모 유래 셀레늄 펩타이드의 인간 섬유아세포에 대한 UVB 보호효과
이향복(Hyang-Bok Lee),이정옥(Jung-Ok Lee),호앙구엔(Dung H. Nguyen),윤선아(SunA Yoon),엄지민(Jimin Um),이유리(Yu Ri Lee),문형인(Hyung-In Moon),정진호(Jin Ho Chung),김은기(Eun-Ki Kim) 한국생물공학회 2009 KSBB Journal Vol.24 No.5
셀레늄 함유 펩타이드 (셀레늄 펩타이드)는 무기 셀레늄이 포함된 배지에서 효모를 배양하여 효모의 자가분해에 의해 만들었다. 효모 배양에 의해 만들어진 셀레늄 펩타이드는 GPx 유사 활성을 보였으며, UVB 조사가 된 인간 섬유아세포에 대하여 세포 보호효과를 나타냈다. 셀레늄 나이트레이트는 10<SUP>-9</SUP> 몰 농도에서 낮은 세포독성을 보인반면 셀레늄 펩타이드는 최소의 독성만을 보였다. 또한 셀레늄 펩타이드는 인간 섬유아세포의 성장과 procollagen type I을 증가시킨 반면 MMP-1의 감소를 가져왔다. 연구결과 셀레늄 펩타이드가 무독성의 항산화제로서의 가능성을 보여주었다. Selenium-containing peptide (Selenium peptide) was produced by autolysis of Saccharomyces cerevisiae which was cultured in inorganic selenium-supplemented medium. Selenium peptide showed antioxidant activity and protective effects on UVB irradiated human fibroblast. Minimal toxicity of selenium peptide was observed whereas selenium nitrate exhibited cell toxicity as low as 10<SUP>-9</SUP> M. Selenium peptide also increased human fibroblast growth, procollagen type I and also decreased MMP-1 (matrix metalloprotease-1). This result showed the potential of selenium peptide as a nontoxic antioxidant.
이향복 ( Hyang-bok Lee ),이행병 ( Haeng Byoung Lee ),이처영 ( Cheo Young Lee ),김은기 ( Eun-ki Kim ) 대한화장품학회 2007 대한화장품학회지 Vol.33 No.4
멜라닌은 UV로부터 피부를 보호하는 기능을 하며, 인종과 피부색을 결정하는 요인이다. 또한 미백 기능성 화장품개발의 주요 목표물이기도 하다. 최근 미와 관련된 화장품 시장이 급성장 하면서 미백효능 증대를 위한 연구들이 활발히 진행되고 있고, 보다 안전하고 효능을 극대화하기 위한 미백 기성화장품의 개발은 관심업계의 주력 분야이다. 또한 경쟁력을 확보하기 위한 수단으로 지속적인 특허 출원이 따르고 있다. 특허는 관련업계의 연구경향을 파악할 수 있는 중요한 문헌이기도 하다. 본 논문에서는 미백 기능성화장품과 관련된 특허 분석을 통하여 관련 업계의 미백연구기술의 개발 동향을 소개하고 미백 기능성 화장품의 개발 및 활성성분의 원료개발에 대한 이해를 돕고자 한다. Melanin plays an important role in protecting human skin from UV radiation and determines the race and skin color. Melanin is also major target for developing skin-whitening cosmeceuticals. Recently, as the market size of skin-whitening cosmeceuticals has rapidly expanded, related researches and developments are also focused on maximizing the safety and efficacy. Also, patents of skin-whitening materials have been increasing steadily for ensuring the competitive power. Patent also shows the research trend of industry and institutes. In this review, we analyze the trend of research and development based on the patent application of skin-whitening cosmeceuticals.
고등식물로부터 Chitin Synthase Ⅱ 활성 저해물질의 탐색
황의일(Eui Il Hwang),이향복(Hyang Bok Lee),김성욱(Sung Uk Kim) 대한약학회 1999 약학회지 Vol.43 No.4
Chitin is an important structural component of fungal cell wall and is synthesized by chitin synthase Ⅰ, Ⅱ, and Ⅲ. The chitin synthase Ⅱ is an essential enzyme for the formation of primary septum in Saccharomyces cereviside. Therefore, specific inhibitors of this enzyme might block the formation of fungal cell wall and could be used as effective antifungal agents. To search chitin synthase Ⅱ inhibitors from natural products, 67 plants were extracted with methanol and examined for the inhibitory activities against chitin synthase Ⅱ of S. cerevisiae by our cell free assay system. As a result, the extracts from 16 plants showed more than 70% inhibition at the concentration of 280mcg/ml. Of note, Laurus nobilis (81.4%), Lonicera maackii (81.5%), Berchemia berchemiaefolia (82.9%), Koelreuteria paniculata (87.9%), Chamaecyparis pisifera (86%) and Taxus cuspidata (83.9%) inhibited strongly the chitin synthase Ⅱ activity.
Birendra Kumar Singh,비비크,이향복,김준섭,김은기 한국화학공학회 2017 Korean Journal of Chemical Engineering Vol.34 No.12
Prunus spp. and locally available plants (used as folkloric medicine) were screened to find a novel and natural anti-melanogenic agent. Based on p-protein promoter reporter assay (PPRA) the candidate plants were screened in the quest for p-protein inhibitor. Expression profiling of key proteins revealed the molecular mechanism of the melanin inhibition as well as TEM analysis revealed melanosome structure. The screened plant extract through PPRA showed significant down regulation of p-protein, which led to melanin inhibition. Another key melanosomal protein like tyrosinase and TRP-1 was also found to be down-regulated. However, TRP-2 was not affected. TEM analysis of treated cells also revealed that the stage IV melanosomes were lowered in number compared to control. The present study shows the plants used in this study possess good anti-melanogenic properties. However, the P. davidiana has the highest anti-melanogenic property among screened plant extracts.
Depigmenting effect of Sterculia lynchnophera on B16F10 melanoma and C57BL/6 melan-a cells
Uyen Do Phuong Lam,김은기,호앙구엔,이향복,Bora Kim,Joo-Dong Lee,Jeong-Hyun Shin 한국화학공학회 2011 Korean Journal of Chemical Engineering Vol.28 No.4
To develop a novel skin depigmenting agent from natural sources, the inhibition of melanogenesis by the Chinese herb, Sterculia lynchnophera (SL), was evaluated. Treatment of B16F10 melanoma cells and melan-a cells with SL exhibited a 32.9% and 68.2% inhibition of melanin synthesis without cytotoxicity at a concentration of 200μg/ml, respectively. This herb possessed a high free radical scavenging activity with IC_50=11.02 μM. The methanol extract of SL slightly inhibited in vitro mushroom tyrosinase activity (23.4% at a concentration of 200 μg/ml) and had a significant inhibitory effect on cellular tyrosinase activivity (48.65% and 88.56% inhibition at the concentration 200μg/ml in B16F10 cells and C57BL/6 melan-a cells, respectively). From the western blotting results, SL inhibited the expression of tyrosinase and tyrosinase related protein 1 (TRP-1). Taken together, we suggest that SL may be a safe and effective depigmentation agent.
한정선 ( Jung-sun Han ),곽은영 ( Eun-young Kwak ),이향복 ( Hyang-bok Lee ),신정현 ( Jung-hyun Shin ),백승학 ( Seung-hak Baek ),정봉현 ( Bong-hyun Chung ),김은기 ( Eun-ki Kim ) 대한화장품학회 2004 대한화장품학회지 Vol.30 No.4
MITF는 미백관련 유전자의 대표적인 조절 인자 단백질로서 미백관련 유전자의 E-box와의 결합정도를 단백질 칩을 이용하여 측정하였다. 융합 단백질 형태의 MITF를 유리 칩에 고정시켰고 E-box를 포함하는 DNA oligomer가 결합하는 것을 확인하였다. 형광법, SPR (surface plasmon resonance), SPRi (surface plasmon resonance imaging)방법 중 형광법이 가장 효과적이었으며, DNA 저해제를 사용시 결합이 감소하는 것을 확인하였다. 이 결과 MITF를 이용한 미백원료의 고속스크리닝(HTS)의 가능성을 보여주었다. An attempt was made to develop a proteinchip for screening of MITF (microphthalmia transcription factor) inhibitor. Binding of MITF to E-box causes transcription of several pigmenting genes including tyrosinase gene. We investigated binding of MITF and its DNA binding site (E-box) using a protein-DNA chip with various detection methods including flurorescence (Cyt3). SPR (surface plasmon resonance) and SPRi (surface plasmon resonance imaging). A fusion protein (MITF-Maltose Binding Protein) was attached on the glass plate by chemical modification. An inhibitory synthetic DNA oligomer, artificially designed based on the E-box sequence, inhibited the binding of MITF and E-box. These results showed the potentials of flurorescence-based MITF protein chip as a microarray for high throughput screening (HTS) system of depigmenting agents.
Enhancing the sporulation of Streptomyces kasugaensis by culture optimization
Won-Bok Chae,Young-Bum Kim,Sung-Won Choi,이향복,김은기 한국화학공학회 2009 Korean Journal of Chemical Engineering Vol.26 No.2
To be an effective microbial biocide, Streptomyces kasugaensis should be converted into spore during cultivation process for successful long-term storage. By statistical design methods, culture conditions including medium components and operating parameters were optimized and more than 100 times increase in spore yield was achieved. Addition of spent culture fluid (100 ppm), EDTA (30 ppm), mycophenolic acid (32 ppm) with combination of pH upshock (5.5 to 8.5) increased total viable cell and spore conversion rate, resulting in 1.6×107 (spore/mL) in 5 days of culture in a fermenter. This result provides a practical method for obtaining high spore number for commercial production of Streptomyces kasugaensis as a microbial pesticide.