문화적 재생을 위한 장소만들기 연구

이학모 세명대학교 인문사회과학연구소 2022 人文 社會科學硏究 Vol.30 No.2

· Research topics: A study on cultural regeneration limits and solutions · Research background: Cultural regeneration is also biased toward the physical environment, revealing problems, and a strategic approach to solving the problem is required. · Difference from prior research: Until now, cultural regeneration only emphasizes the necessity of resource utilization and space creation, and overlooks the limitation of surface regeneration. · Research method: We will try to find a strategic approach to cultural regeneration by analyzing theoretical approaches to place making and cultural regeneration and specific field cases. · Research result: The cultural approach can also be a means to sustain the regeneration performance, and the usefulness of the place-making approach is suggested as a method for this. · Contribution points and expected effects: While revealing the usefulness of the approach to cultural regeneration, he suggested a place-making approach as a practical application method rather than the existing method that focused only on resources or space. Proposal of the possibility of an individualistic approach to spatial content planning and operating system for actual field application in the future · 연구주제: 문화적 재생 한계와 해결방안 연구· 연구배경: 문화적 재생도 물리적 환경에만 치우친 나머지 문제점이 드러나고 있으며, 문제해결에 대한 전략적 접근이 요구· 선행연구와의 차이점: 지금까지 문화적 재생은 자원 활용과 공간조성의 당위성만 강조할 뿐 표면적 재생에 그칠 한계를 간과하고 있으며, 장소만들기도 구성과 특성에만 집중해 현실적용 방안 미흡· 연구방법: 장소만들기와 문화적 재생에 대한 이론적 접근과 구체적인 현장 사례를 분석해 문화적 재생에 대한 전략적 접근을 모색· 연구결과: 문화적 접근도 재생성과를 지속 시킬 수단이 될 수 있으며, 이를 위한 방안으로 장소만들기 접근의 유용함을 제시· 공헌점 및 기대효과: 문화적 재생의 접근방식 유용함을 밝히면서 현실적용 방안으로 자원이나 공간에만 집중했던 기존 방식이 아닌 장소만들기 접근을 제시했다. 향후 실제 현장 적용을 위한 공간 콘텐츠 기획과 운영시스템에 대한 각론적 접근의 가능성 제시

기니픽, 마우스 그리고 랫드에서 EPO (Erythropoietin)의 항원성에 관한 고찰

이학모,남정석,제정환,이석만,양재만,강병철,박재학,송동호,유선희 한국독성학회 1997 Toxicological Research Vol.13 No.1

The antigenicity of EPO (erythropoietin) was investigated in guinea pig, mice and rats. Antigenicity tests-active systemic anaphylaxis (ASA), passive cutaneous anaphylaxis (PCA) of this materials were performed according to the established Regulation of Korean National Institute of Safety Research (1996, 4, 16). The results were followed: 1. After sensitizaion with EPO emulsified with complete Freund's adjuvant (CFA), guinea pigs didn't show any anaphylatic shock symptom in the ASA test 2. After sensitization with antisera of EPO sensitized mice, blue spots were observed on the hypodermis of back of rats in the PCA test, but diameter of each spot was smaller than 5 mm. From the results of this investigation, the antigenicity of EPO was negative under the present experimental condition.

Optimal developmental stage of Sertoli cells for maximum immune protection

이학모,오병철,권경진,이미정,임동표,이정렬 한국실험동물학회 2005 한국실험동물학회 학술발표대회 논문집 Vol.2005 No.-

Mechanism of Humoral and Cellular Immune Modulation Provided by Porcine Sertoli Cells

이학모,오병철,Dong Pyo Lim,이동섭,Hong Gook Lim,Chun Soo Park,이정렬 대한의학회 2008 Journal of Korean medical science Vol.23 No.3

The understanding of main mechanisms that determine the ability of immune privilege related to Sertoli cells (SCs) will provide clues for promoting a local tolerogenic environment. In this study, we evaluated the property of humoral and cellular immune response modulation provided by porcine SCs. Porcine SCs were resistant to human antibody and complement-mediated formation of the membrane attack complex (38.41±2.77% vs. 55.02±5.44%, p=0.027) and cell lysis (42.95±1.75% vs. 87.99 ±2.25%, p<0.001) compared to immortalized aortic endothelial cells, suggesting that porcine SCs are able to escape cellular lysis associated with complement activation by producing one or more immunoprotective factors that may be capable of inhibiting membrane attack complex formation. On the other hand, porcine SCs and their culture supernatant suppressed the up-regulation of CD40 expression (p<0.05) on DCs in the presence of LPS stimulation. These novel findings, as we know, suggest that immune modulatory effects of porcine SCs in the presence of other antigen can be obtained from the first step of antigen presentation. These might open optimistic perspectives for the use of porcine SCs in tolerance induction eliminating the need for chronic immunosuppressive drugs.

Role of Complement Regulatory Proteins in the Survival of Murine Allo-transplanted Sertoli Cells

이학모,오병철,Dong Pyo Lim,이동섭,조재진,이진,이정렬 대한의학회 2007 Journal of Korean medical science Vol.22 No.2

Sertoli cells (SC) are known to contain immunoprotective properties, which allow them to survive as allografts without the use of immunosuppressive drugs. Experiments were designed to determine which factors are related to prolonged survival of allogeneic SC. Balb/c derived Sertoli (TM4) and colon cancer (CT-26) cell lines were implanted beneath the kidney capsule of non-immunosuppressed C57BL/6 mice and compared their survival as allografts. Compared to TM4 graft, which survived more than 7 days after transplantation, CT-26 showed massive infiltration of polymorphonuclear cells, necrosis and enlargement of draining lymph nodes. Cultured cell lines showed no differences in their expression patterns of FasL, TGF 1, clusterin and two complement regulatory proteins (CRP, i.e., membrane cofactor protein, MCP; decay accelerating factor, DAF), but protectin (CD59), another member of CRP was expressed only on TM4. These results suggest that CD59 and unknown factors may contribute to the prolonged survival of SC in non-immunoprivileged sites.

Neonatal Hypothyroidism : Useful Approach in Sertoli Cell Primary Isolation

이학모,임동표,오병철,권경진,이미정,이정렬 한국실험동물학회 2005 한국실험동물학회 학술발표대회 논문집 Vol.2005 No.-

Effect of Hyperkalemia and Hemolysis Caused by Hyperacute Rejection on Cardiac Function in Pig to Human Ex Vivo Xenogeneic Cardiac Perfusion Model

김준석,이학모,오병철,임홍국,이정렬,김웅한 대한심장학회 2011 Korean Circulation Journal Vol.41 No.3

Background and Objectives: Hyperacute rejection (HAR) is a major obstacle to successful xenotransplantation of vascularized organs. This study was conducted to observe the effect of hemolysis of perfused human whole blood on pig heart function, and determine the major risk factors for preservation of xenoperfused cardiac function using ex-vivo pig to human xenogeneic cardiac perfusion model. Materials and Methods: Harvested pig hearts were perfused with normal human whole blood (group 1), two different types of pre-treated human whole blood (group 2: immunoglobulins were depleted by plasmapheresis, group 3: pre-treated with plasmapheresis, GAS914, cobra venom factor (CVF) and steroid), and normal porcine wh-ole blood as control (group 4) for 3 hours. Results: Duration of heart beat was significantly prolonged in group 2 and group 3. Histological examination showed widespread HAR features but was gradually delayed in groups 2 and 3 compared to group 1. The absolute levels of serum creatine kinase-MB and Troponin I increased gradually, and was lower in group 3. Serum hemo-globin levels were rapidly increased in groups 3 and 4, compared to group 1. Extracellular potassium level increased sharply from the beginning of blood perfusion in groups 1, 2 and 3, compared to group 4. Conclusion: Pretreatment of human whole blood, including immunoglobulin depletion, CVF and steroid reduced and delayed the destruction of pig myocardium by HAR. However, the increased extracellular potassium levels in groups 1, 2 and 3 reflected that these treatments could not prohibit myocardial injury by HAR. Transplantation, heterologous; Hyperkalemia; Hemolysis; Extracorporeal circulation.

신규 개인제독키트, SDK시제품(가칭)의 면역독성평가에 관한 고찰

강병철,이학모,제정환,남정석,이석만,양재만,박재학,이영순 한국독성학회 1998 Toxicological Research Vol.14 No.2

To evaluate immunotoxicity of skin decontamination kit(SDK) newly-developed in Agency for Defense Development(ADD), delayed contact hypersensitivity (maximization) test and passive cutaneous anaphylaxis(PCA) test of SDK were performed and the results were compared with those of M 291. In maximization test, sensitization reaction was induced by id injection (2.5 mg / 0.1 $\textrm{m}{\ell}$/ guinea pig or 2.5 mg+CFA/0.1 $\textrm{m}{\ell}$/guinea pig) and topical application (2.5 mg/$\textrm{m}{\ell}$/guinea pig) with SDK or M291 at an interval of 1 week, and 2 weeks later, challenged by topical application with 25 mg/$\textrm{m}{\ell}$/guinea pig. SDK and M291 did not induce any reactions, showing 0 point of sensitization score and 0% of sensitization rate. In conclusion, it is suggested that SDK and M291 do not induce delayed contact hypersensitivity. In PCA test, rats were administered id with mouse anti-SDK serum and challenged iv with a mixture of antigen SDK and Evan's blue. SDK did not induce blue spots at the injection sites of both high (2.5 mg/mouse) and low (1.25 mg/mouse) dose-induced antisera. In contrast, BSA, positive control produced spots larger than 5 mm in diameter at the injection sites of BSA-induced antiserum up to $2^2$ ~ $2^4$dilution. In conclusion, it is suggested that SDK do not induce IgE production and is not a PCA-reaction inducer.

Kinetics of IFN-γ and IL-17 Production by CD4 and CD8 T Cells during Acute Graft-versus-Host Disease

주지민,이학모,오근희,이동섭,최은영 대한면역학회 2014 Immune Network Vol.14 No.2

Graft-versus-host disease (GVHD) is a fatal complication that occurs after allogeneic hematopoietic stem cell transplantation. To understand the dynamics of CD4 and CD8 T cell production of IFN-γ and IL-17 during GVHD progression, we established a GVHD model by transplanting T cell-depleted bone marrow (TCD-BM) and purified T cells from B6 mice into irradiated BALB.B, creating an MHC-matched but minor histocompatibility (H) antigen-mismatched transplantation (B6 → BALB.B GVHD). Transplantation-induced GVHD was confirmed by the presence of the appropriate compositional changes in the T cell compartments and innate immune cells in the blood and the systemic secretion of inflammatory cytokines. Using this B6 → BALB.B GVHD model, we showed that the production of IFN-γ and IL-17 by CD4 T cells preceded that by CD8 T cells in the spleen, mesenteric lymph node, liver, and lung in the BALB.B GVHD host, and Th1 differentiation predated Th17 differentiation in all organs during GVHD progression. Such changes in cytokine production were based on changes in cytokine gene expression by the T cells at different time points during GVHD development. These results demonstrate that both IFN-γ and IL-17 are produced by CD4 and CD8 T cells but with different kinetics during GVHD progression.

인터넷 정보망을 이용한 군 건강 및 생산관리수의학

김선진,이학모,손우찬 대한수의사회 2000 대한수의사회지 Vol.36 No.11

본 란에서는 지난 호의 군 건강 및 번식 관리 프로그램의 원리에 이어서, 이 프로그램을 효율적인 기록 관리에 대해서 기술하고자 한다. 기록이라 하면 단순한 사실의 나열을 넘어서 자료들을 통합하고 가공하며 유용한 형태로 보관하는 것을 의미한다. 또한 기록은 수의사나 농부의 능력을 가늠하는 척도로 여겨질 만큼 중요하다. 여기서는 무엇을 기록해야 하는가 하는 점에서부터 자료의 저장 방법 및 다양한 시스템들, 특히 컴퓨터 이용의 중요성에 대해서 설명하기로 한다.