질산염과 인산염 농도 변화에 따른 Cymnodinium sanguineum과 Skeletonema costatum의 성장과 생화학적 구성성분의 비교

이택견,박명환,신경순,장만 한국환경생물학회 2000 환경생물 : 환경생물학회지 Vol.18 No.4

Growth and biochemical composition were analyzed in Gymnodinium sanguineum and Skeletonema costatum grown in media containing various nitrate and phosphate concentrations. Concentrations of nitrate and phosphate in the growth media were 0, 0.3, 0.6, 0.9, 1.2mM and 0, 15, 30, 45, 60 μM, respectively. Growth of G. sanguineum was suppressed in the low concentration of nitrate and phosphate(below N=0.3mM and P=15μM), whereas growth of S. costatum did not changed. At the low concentrations of nitrate and phosphate, amount of intracellular protein and carbohydrate in G. sanguineum cells were largely decreased, whereas content of carbohydrate in S. costatum cells was increased little. Amount of neutral lipid and phospholipid in G. sanguineum didn't chagned, but concentration of glycolipid was largely decreased in the medium containing low concentrations of nitrate and phosphate. However the levels of TAG, glycolipid, and phospholipid did not changed in S. costatum cells. These results show that S. costatum is more adaptable than G. sanguineum in the low concentration of nitrate and phosphate.

Acetate와 Glucose가 혼합되어 있는 배지에서 성장하는 당근 현탁배양 세포의 diauxic growth

李宅堅,李宇成 성균관대학교 기초과학연구소 1995 論文集 Vol.46 No.2

우리는 이제까지 bacteria와 yeast 등에서 관찰되어 온 diauxic growth 현상이 식물에서도 관찰되는지에 대해 연구해왔다. 우리는 이미 단자엽(벼) 현탁배양을 acetate와 glucose 가 혼합되어 있는 배지에서 성장시켰을 때 diauxic growth 현상이 있음을 보고하였다 (1). 우리는 쌍자엽식물에서도 diauxic growth가 관찰되는지 알아보기 위하여 당근의 현탁배양을 이용하여 연구하였다. 당근의 현탁배양세포를 glucose와 acetate가 혼합되어 들어있는 배지에서 성장시켰을 때 diauxic growth가 관찰되었다. Diauxic growth의 첫 번째 성장기에 acetate를 사용하고 두 번째 성장기에 glucose를 사용한다. 첫 번째 성장기에 있는 세포는 [^14C]acetate를 흡수하지만 [^14C]glucose 는 흡수하지 못했다. [^14C]glucose 흡수는 두 번째 성장기 세포에서 관찰할 수 있었다. 벼의 현탁배양 세포와 마찬가지로 당근의 현탁배양세포도 acetate를 일차적인 탄소원으로 사용하는 diauxic growth가 관찰되었다. We have been investigating acetate-glucose diauxic growth in plant. It was reported that rice (monocotyledon) suspension cells were exhibited diauxic growth on acetate (10 mM) and glucose (10 mM) medium (1). To investigate diauxic growth in dicotyledoneous plant, carrot (Daucus carota L.) suspension cells were grown on acetate and glucose medium Suspension culture of carrot exhibited diauxic growth when grown in a medium containing both acetate and glucose as carbon sources. Preferential utilization of acetate was observed in the initial growth phase of diauxic growth, whereas the second growth phase occured at the expense of glucose. Cells harvested during the first growth phase exhibited a capacity uptake of [^14C]acetate but not of [^14C]glucose. A capacity for [^14C]glucose uptake was expressed during the second growth phase. Like in rice suspension cells, in carrot suspension cells acetate was used as the primary carbon source during the acetate-glucose diauxic growth.

High light에 의한 식물플랑크톤의 reactive oxygen species (ROS) 생성과 적응기작

이택견(T.K. Lee),김선주(S.J. Kim),신경순(K.S. Shin),장만(M. Chang) 한국해양환경·에너지학회 2000 한국해양환경공학회 학술대회논문집 Vol.2000 No.-

치과용 부식제가 백서 법랑질 표면에 미치는 영향에 대한 주사전자현미경적 관찰

權宅見 大韓齒科保存學會 1980 Restorative Dentistry & Endodontics Vol.6 No.1

ABSTRACTIn this experiment, etching was performed on the incisor enamel surfaces of IS; Albino rats, weighted about ISOgm, with both 30% and 50% concentrations of the phosphoric, citric acids, and EDTA solution for 60 seconds.Immediately after extraction of the animal teeth, laboratory procedures for scanning electron microscopic observations were made and the author derived the following conclusions: 1. On the enamel surface treated with 30% and 50% phosphoric acids, all of the periphery, center of the enamel rods, and the enamel matrices were severely etched. 2. In the etched with 30% citric acid group, only the small part of the enamel rods were etched out. In case of 50% citric acid the periphery, center of enamel rods were- partially or entirely etched, and the intermatrix spaces were widened. 3. In the 30% and 50% of the EDTA etched groups, the periphery of the enamel rods, explicitely were etched, and the spaces between matrices were widened.

천연 해조류로부터 적조생물 살생제 개발

이택견(T.K Lee),신경순(K.S. Shin),장만(M. Chang) 한국해양환경·에너지학회 1999 한국해양환경공학회 학술대회논문집 Vol.1999 No.-

Development of biomarkers for risk assessment of marine organisms

Taek-Kyun Lee(이택견),Seonock Woo(우선옥),Seungshic Yum(염승식),Sojung Kim(김소정),Eun Seok Choi(최은석),Rora Oh(오로라) 한국생물공학회 2004 한국생물공학회 학술대회 Vol.2004 No.10

人參과 獨活의 原形質體 融合에 影響을 미치는 諸 要因

이재두,이택견 성균관대학교 1991 論文集 Vol.42 No.1

두 원형질체 사이의 융합에 PEG, PEG 용액내의 Ca^2+ 이온 농도, DMSO의 농도, 처리 시간, eluting 용액 내에서의 pH, Ca^2+ 이온 농도 등이 융합빈도에 영향을 미치는데 가장 높은 융합빈도는 25% PEG 6000, 10mM의 CaCl_2·2H_2O, 2% DMSO, 그리고 eluting 용액내에 pH 10.5, 55mM CaCl_2·2H_2O를 사용했을 때 얻을 수 있었다. 그러나 높은 분자량(PEG 8000)과 고농도(30% 이상)의 PEG, 2.5% 이상의 DMSO 및 25분 이상의 처리시간은 원형질체의 활성도에 악영향을 미친다. Factors affecting fusion frequency of protoplasts Panax ginseng calli and Aralis continentalis mesophyll cells were studied. Using the enzyme solution of 0.5% macerozyme 'Onozuka' R-10 and 15% cellulase 'Onozuka' R-10, Panax ginseng protoplasts were isolated. And, Using the enzyme solution of 1.0% macerozyme 'Onozuka' R-10 and 2.0% cellulase 'Onozuka' R-10, Aralis continentalis protoplasts were isolated. The fusion frequency was depended upon (1) polyethylene glycol(PEG), (2) Ca^2+ concentration in the PEG solution, (3) concentration of DMSO, (4) incubation time, (5) pH in the eluting solution, and (6) Ca^2+ concentration in the eluting solution. The highest fusion frequency between two kinds of protoplasts was obtained when protoplasts were treated with 25% PEG(MW 6,000), 2% DMSO, and 10mM CaCl_2·2H_2O for 15-20 minutes and eluted by eluting solution supplemented with 55mM CaCl_2·2H_2O at pH 10.5. But, when protoplasts were incubated in solution with high MW(PEG 8,000) and high concentration(more 30%) of PEG and more than 2.5% DMSO for more 25 minutes, protoplast viability was decreased.

영양염 변화에 따른 식물플랑크톤의 성장과 세포 구성성분

신경순,이택견,박명환,장만 한국해양환경·에너지학회 2000 한국해양환경공학회 학술대회논문집 Vol.2000 No.-

벼의 엽신 및 캘러스에서 Cytokinin 유도성 A-type 및 C-type Cyclin 유전자의 발현 분석

이홍근,이택견,이석찬,최승호,오정균,황현식,박정안,박종범 한국식물생명공학회 2005 JOURNAL OF PLANT BIOTECHNOLOGY Vol.32 No.1

The expression patterns of cyclin genes, which play a crucial role on cell cycle control, were analyzed with rice calli and leaf blades from seedlings. When callus was transferred from media containing the combinations of 2,4-D and kinetin under the dark conditions to medium supplemented with cytokinin-only on 7 days after the cultures, the expression levels of A-, B- and C-type cyclins from callus were increased significantly. Despite the fact that cyclin genes were well expressed on leaf blades rather than other organs in rice seedlings, rice leaf blades grown on the medium containing various combinations and concentrations of cytokinin for 24 hours had no major effect on the expression patterns of cyclins except zeatin. The relation between cytokinin regulation and the expression of cyclins of rice is discussed. 세포 주기의 조절에 있어 중요한 역할을 담당하고 있는 cyclin 유전자들을의 발현 양상을 벼에서 유도한 캘러스와 엽신을 이용하여 관찰하였다. 특히 캘러스를 이용한 분석 결과 2,4-D와 kinetin이 조합된 배지 위에 7일 동안 암 배양한 후 이를 cytokinin만이 첨가된 배지로 옮겼을 때 A-, B-, C-type cyclin 유전자들의 발현이 더 증가되었다. 또한 벼 실생의 각 기관에서 cyclin 유전자의 발현양을 조사한 결과 다른 기관들에 비해 잎에서 A-, B, C-type cyclin 유전자들이 더 많이 발현하였으나 잎에 cytokinin의 종류와 농도를 다르게 처리하여 24시간동안 배양한 결과에서는 zeatin을 제외하고는 cytokinin의 종류와 양이 cyclin 유전자들의 발현에 큰 영향을 미치지 못함을 알 수 있었다. 본 실험에서는 벼 cyclin 유전자의 발현과 식물생장조절제 처리와의 상관 관계에 대해서 조사하였다.

적조원인종인 Prorocentrum minimum의 광도에 따른 생화학적 조성의 변화

조진하,이택견,신경순,이우성,장만 한국환경생물학회 1998 환경생물 : 환경생물학회지 Vol.16 No.4

적조원인종인 Prorocentrum minimum의 생화학적 조성의 변화에 대한 광도의 영향을 조사하였다. 성장조건은 14:10h(L:D)로 설정하였으며, 15, 50, 120μ㏖·m^-2·s^-1의 광도범위에서 실험을 수행하였고, 지수함수기에 세포를 모아 생화학적 구성성분을 분석하였다. 50μ㏖·m^-2·s^-1 이상에서는 성장유형이 거의 유사하였고, 세포내 단백질, 탄수화물 및 지질의 양은 각각 120, 50, 15μ㏖·m^-2·s^-1에서 가장 많이 존재하는 것으로 나타났다. 단백질/탄수화물의 비율과 MGDG/DGDG의 비율은 50μ㏖·m^-2·s^-1에서 가장 높았으며 photosystem Ⅱ의 활성도는 120μ㏖·m^-2·s^-1 이상의 광도에서 성장한 세포에서 가장 낮았다. 따라서 50μ㏖·m^-2·s^-1의 광도에서 성장한 세포가 세포활성도 및 광합성능이 가장 높은 것으로 관찰되었다. Effects of light intensity on the biochemical composition of Prorocentrum minimum, a dinoflagellate causing red tide, were examined. Cultures were grown under a 14:10h light: dark(L:D) regime at three light intensity ranging from 15 to 120μ㏖·m^-2·s^-1 and harvested at late-logarithmic phase for analysis of biochemical composition. Growth pattern was similar to over the 50μ㏖·m^-2·s^-1. The highest levels of carbohydrate, protein and lipid were present in the cells from cultures grown at 120, 50 and 15μ㏖·m^-2·s^-1, respectively. The protein/carbohydrate ratio and MGDG/DGDG ratio was highest in cells from culture grown at 50μ㏖·m^-2·s^-1. The activity of photosystem Ⅱ of the culture grown 120μ㏖·m^-2·s^-1 was lower than others. Therefore the cells grown 50μ㏖·m^-2·s^-1 represented highest cellular activity and photosynthetic activity.