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닭의 인공수정에 있어서 적정 주입 정자수 및 주입간격에 관한 연구
이재근,안해범,정선부 ( J . K . Lee,H . B . Song,S . B . Chung ) 한국축산학회 1978 한국축산학회지 Vol.20 No.1
This experiment was carried out to determine the optimum amount of semen (sperm number) to use per insemination and intervals between insemination in artificial insemination of hens. The experimental birds used were 240 hens and cocks of White Plymouth Rock inbred line at 7 months of age. The four treatment compared were: 20 million sperms per 0.005 ㎖ (T1), 40 million sperms per 0.01 ㎖ (T2), 80 million sperms per 0.02 ㎖ (T3) and 120 million sperms per 0.03 ㎖ (T4). Thirty birds were allotted per treatment within a replication, and there were two replications in the experiment. Ringer`s Solution was used for diluent of semen (1 : 3), and semen was inseminated within 30 minutes after collection. The interval between inseminations was 5 days. The results obtained are summarized as follows: 1. The fertility for 5 days after second insemination was 58.2% for T1, 73.1% for T2, 75.1% for T3 and 77.8% for T4. The differences among 72, T3 and T4 were statistically insignificant. 2. In order to obtain a maximum level of fertility in a flock with low egg production and low fertility such as the flock used in this study, the optimum internal between inseminations appears to be 2 days for T2 and 3 days for T3 and T4.
이재근,송해범,정선부,홍기창 ( J . K . Lee,H . B . Song,S . B . Jung,K . C . Hong ) 한국축산학회 1980 한국축산학회지 Vol.22 No.2
This experiment was carried out to determine the optimum amount of semen (sperm number) to use per injection in artificial insemination of hens. the experimental birds used were 240 hens of White Plymouth Rock inbred line and 20 cocks of Australorp pure line at 7 months of age. The four treatments compared were: 0.005 ㎖ or 16 to 18 million sperm per insemination (T1), 0.01 ㎖ or 32 to 36 million sperm (T2), 0.02㎖ or 64 to 72 million sperm (T3), and 0.03 ㎖ or 96 to 108 million sperm (T4). thirty birds were allotted per treatment within a replication, and there were two replications in the experiment. Ringer`s Solution were used for diluent of semen(1:3), and semen was inseminated within 30 minutes after collection. The hens were inseminated 4 times with the intervals of 4 days. The results obtained are summarized as follows. 1. The fertility for four days after the second insemination was 46.4% for the T1, 72.9% for T2, 73.3% for the T3 and 86.3% for the T4. The fertility of the T1 was significantly lower than the others, but the differences among T2, T3 and T4 were not significant. These results agree with the previous report (1978). 2. The fertility for 4 days after the 3rd insemination was 71.0% for the T1, 73.4% for the T2, 73.3% for the T3 and 77.1% for the T4, and t he fertility after the 4th insemination was 70.4% for the T1, 65.7% for the T2, 69.2% for the T3 and 79.3% for the T4. None of the differences among the treatments were statistically significant in each ease. It was concluded that the insemination of 0,005㎖ (16 to 18 million sperm per insemination) can attain the same level of fertility as the insemination of 0.01 ㎖ to 0.03㎖ after the 3rd or 4th insemination. 3. In view of the results obtained in this study, the least optimum dose of semen in the case of 4 days interval was estimated to be within the range from 0,005㎖ to 0.023㎖
입자 영상 처리 시스템을 이용한 콜로이드 입자의 제타포텐셜 측정 및 나노유체 분산 특성 연구
이재근 ( J. K. Lee ),김성찬 ( S. C. Kim ),김희중 ( H. J. Kim ),이창건 ( C. G Lee ),주찬홍 ( C. H. Ju ),이래철 ( L. C. Lee ) 한국분무공학회 2003 한국액체미립화학회지 Vol.8 No.1
N/A Zeta potential measurements of colloid particles suspended in a liquid are performed by a Zeta Meter developed. There are many applications of colloid stability in spray technology, paints, wastewater treatment, and pharmaceuticalsc. Zeta potentials of charged particles are obtained by measuring the electrophoretic velocities of the particles using video enhanced microscopy and image analysis program. The values of zeta potential of polystyrene latex(PSL), silica(SiO_2), polyvinylidence difluoride(PVDF), silicon nitride, and alumina particles in deionized (DI) water were measured to be -40.5, -31.9, -25.2, -15.1 and -10.1 mV, respectively. The particles having high zeta potential less than -20 mV are stable in DI water, because the double layers of them have strong repulsive forces mutually, and the particles having low zeta potential over -20mV are unstable due to Van Der Waals forces. Silica(>20 nm), PSL, aluminum and PVDF particles were found to be stable that would remain separate and well disperse, while silicon nitride and alumina particles were found to be unstable that would gradually agglomerate in DI water.
닭의 인공주정시각이 수정에 미치는 영향 2 . 천질주정에 관한 연구 ( 제3보 ) - 오전 3시주정과 오후 3시주정과의 비교-
이재근 ( J K Lee ) 한국축산학회 1973 한국축산학회지 Vol.15 No.1
This experiment was carried out to study the fertility comparison between 3 p.m. insemination, what is known to be optimum time for the highest fertility, and 3 a.m. insemination. 257 hens of seven months old Shaver Starcross 288, twenty months old Welph Rock p.s. male birds were used in this trial and 1,380 eggs were tried. The method of insemination used was to deposit 0.04㎖ of undiluted mixed semen, once for each hen, about 2∼3㎝ deep into vagina and following results were obtained; 1. The fertility on the day following insemination was 91.1% for 3 a.m. lot, 81.4% for 7 a.m. lot, 0% for 11 a.m. lot, 0% for 3 p.m. lot and another 0% for 7 p.m. lot. The egg production of the flock was over 90% during the trial. 2. The fertile eggs laid on the day following insemination were obtained mostly from hens inseminated before oviposition, and partly from hens inseminated on the day with no oviposition. Even though the highest fertility can be expected on the day following insemination when insemination takes place 3 to 5 hours before oviposition or ovulation, fairly high fertility still can be obtained by inseminating only 2 to 3 or 1 to 2 hours before the oviposition in high producing and highly fertile flock. It was reported in my previous studies that 3 to 5 hours could be the enough time taken for the sperm to reach utero-vaginal junction from 2∼3㎝ spot in vagina. It is found by my previous and current studies that there is a very distinct difference in the relationship of interval from insemination to oviposition and fertility between high producing and low producing flocks. It is suggested a certain physiological mechanism to accelerate sperm movement in vagina of hen could exist along with the motion of sperms themselves. 3. The fertility during those six days after insemination was 88.9% for 3 a.m. lot, 80.4% for 7 a.m. lot, 72.7% far 11 a.m. lot, 93.6% for 3 p.m. lot, and 88.1% for 7 p.m. lot respectively. Though the highest fertility was observed in 3 p.m. there was no significance when compared with 3 a.m. lot. 4. To obtain higher fertility during those six days after insemination it is generaly recommended to inseminate about 4 hours before oviposition or ovulation, in case of inseminating before oviposition or on the day of no oviposition, however in the flock with high production such as over 90%, 4∼5 a.m. insemination with 2∼3 hours interval could also bring fairly high fertility. In case of inseminating after oviposition a minimum of 2 hours after oviposition was needed to obtain high fertility.
닭의인공주정시각이 수정에 미치는 영향 Ⅱ . 천질주정에 관한 연구 ( 제2보 )
이재근 ( J K Lee ) 한국축산학회 1970 한국축산학회지 Vol.12 No.1
It was suggested in my previous study published in 1968 that higher fertility should be obtained in oviposition before noon when hens were inseminated at early 3 to 5 o`clock. The present experiment was carried out to reexamine my previous study, and 350 hens and 1576 eggs produced by them were used for this experiment. The method of insemination used was to deposit 0.04 ㎖ of undiluted mixed semen, once for each hen, about 2-3 ㎝ deep into vagina. Hens were inseminated at seven different times of the day begining from 11 o`clock p.m., and the fertility of hens inseminated at different time was observed. The results obtained are summarized as follows; The fertility on the day following insemination was 58.3% for 11 p.m. lot, 78.8% for 1 a.m. lot, 81.3% for 3 a.m. lot, 54.8% for 5 a.m. lot, 41.9% for 7 a.m. lot, 24.2% for 9 a.m. lot, 0% for 11 a.m. lot. Higher fertility was obtained in 3 a.m. on the day following insemination proving that interval from insemination to oviposition or to ovulation should be larger than 3-5 hours. It appeared that oviposition after a pause took place at around 7 a.m. in most cases. In case of insemination after oviposition, no fertile egg was obtained on the day following insemination. The average fertility during a week following insemination was 53.3% for 11 p.m. lot, 61.6% for 1 a.m. lot, 71.7% for 3 a.m. lot, 64.4% for 5 a.m, lot, 58.6% for 7 a.m. lot, 56.2% for 9 a.m. lot, 50.7% for 11 a.m. lot, also showing the highest fertility in 3 a.m. lot. The cause of higher fertility in 3 a.m. lot was same as that in the fertility on the day following insemination.
고추 (Capsicum annuum L.) 생육에 미치는 금진광천수의 영향
유지혜 ( J. H. Yoo ),성은수 ( E. S. Seong ),김남준 ( N. J. Kim ),황인성 ( I. S. Hwang ),이재근 ( J. G. Lee ),권순성 ( S. S. Kwon ),김정득 ( J. D. Kim ),최선강 ( S. K. Choi ),심재만 ( J. M. Sim ),길현영 ( H. Y. Kil ),유창연 ( C. Y. Yu 강원대학교 농업생명과학연구원(구 농업과학연구소) 2011 강원 농업생명환경연구 Vol.23 No.3
Geumjin Thermal Water (GTW) from the Geumjin area (Gangneung-city, Korea) has abundance minerals such as selenium, vanadium, calcium and magnesium etc.. In this study, we treated various concentrations of GTW in hot pepper (Capsium annuum L.). GTW treatments of 1-10% showed different phenotypes in plant length, fresh weight, length and width of leaf, color of leaf and number of leaf. The height of plants treated by GTW was taller than control. However, it of them was reduced over 5% GTW treatment. The fresh weight of plant indicated highly as 30.0±0.1g in 5% treatment condition. There was no difference between control and treated plants with GTW in the width of leaf. But, the length of leaves showed highest in 5% (9.2±0.6cm) treatment condition. From these results, we consider that GTW showed the effect on initial biomass of plants.
생물반응기 배양에 의한 산삼 수확 후 배양액이 무의 초기 생장에 미치는 효과
유창연 ( C. Y. Yu ),이재근 ( J. K. Lee ),성은수 ( E. S. Seong ),김병준 ( B. J. Kim ),권순성 ( S. S. Kwon ),고은정 ( E. J. Gho ),안형신 ( H. S. Ahn ),이종윤 ( J. Y. Lee ) 강원대학교 농업생명과학연구원(구 농업과학연구소) 2009 강원 농업생명환경연구 Vol.20 No.-
In order to investigate the optimal conditions for using recycled liquid medium after cultured a wild Panax ginseng C. A. Meyer, we examined the effect of 100% distilled water and several diluted culture medium on the growth of radish seedlings. Of six conditions tested, in 1:3diluted culture medium, fresh weight was extremely higher and 141.42 g while in distilled water the fresh weight was the lowest. Dry weight of radish was higher in condition of 100% culture medium and as the ratio of cultured medium increased, the dry weight of radish was gradually increased. In this results, culture medium was effective on the growth, fresh weight and dry weight of radish. These results suggested that recycled culture medium of Panax ginseng adventitious roots could be used for producing vegetable radish sprouts, and improving their the functional activity.