단완 12부위에서 장완 13부위의 완간역위를 가진 22번 염색체 모자이크삼체성

최인장(Choi In Jang),이인환(Lee Ihn Hwan),고복현(Ho Bok Hyun),장성익(Chang Sung Ik) 대한체질인류학회 1991 대한체질인류학회지 Vol.4 No.2

정상분만과 정상의 신체발육한 11세 환아의 주된 증상은 지능장애였으며 그의 관찰된 임상소견으로 눈꺼풀 틈새의 하사성, 넓고 납작한 코, 긴 인중, 작은 하악골 및 비정상적인 귀여다. 사지와 생식기에는 전혀 이상이 없었으며 흉부와 복부에도 특이할 만한 이상은 발견되자 않았다. 말초혈액세포를 이용한 Gㆍband와 NORㆍband로 22번 염색체 모자이크 삼체성이였으며, 특이한 22번 염색체는 일반적인 길이와 완비(arm ratio)를 기초로 하여 단완 12부위에서 장완 13 부위의 완간역위를 가진 산물로 밝혀졌다.

癌細胞 染色體 硏究에 있어서 새로운 方法 : 細胞分離와 培養方法 Cell segregation and culture technique

張性翼,崔寅章,李仁煥 啓明大學校 醫科大學 1986 계명의대학술지 Vol.5 No.1

固形質 종양 조직의 細胞 分離에 있어서 物理的인 方法과 物理的인 方法에 효소 처리를 추가한 세 가지 方法으로 細胞 分離를 한 결과 요약하면 다음과 같다. 1. 복합 효소 처리에서 가장 높은 生存力 있는 分離 細胞群(cell yield)을 얻었으며 5.4~5.8×10), 物理的인 方法에서 가장 낮은 分離 細胞群(0.08~0.4×10)을 얻었다. 2. 세 가지 癌 조직에 대한 네 가지 方法으로 細胞를 分離해 본 결과 각기 方法마다 자궁 경부암에서 가장 높은 分離 細胞群을 얻을 수 있었다. Three combined mechanical and enzymatic disaggregatioin techniques and a simple mechanical disaggregation procedure were compared. The combined procedures involved a mechanical comminution the tumour tissue followed by incubation in trypsin. In one method, the tissue was subjected to log-term trypsinzation at 4℃, and in the other procedure, repeated short-term trypsinization at 37℃, and in the third method, trypsinization at 4℃ and collaganase procedure was applied: The results were compared in terms of the yield of viable cells. The combined techniques provided reproducible cell yields of 2.8-5.8×10? viable cells g-¹of tissue, whereas only a small number of tumour cells was produced by the mechanical method. Especially, after trypsinization at 4℃ and collagenase procedure, we gained high cell yield of 5.4-5.8×10? viable cells g-¹of tissue. We introduce this advanced technique on tumour cell to detact the prognosis of patients suffered from neoplasm clinically.

Chlorambucil 이 수컷 생쥐의 생식세포에 미치는 영향

장성익,최인장,김곤섭,김덕훈,이인환 啓明大學校 醫科大學 1987 계명의대학술지 Vol.6 No.2

The present study was performed to investigate the effects of chlorambucil on the reproductive cells of male mice. The animal was treated with daily injection of chlorambucil (16mg/kg) for periods up to 35 days. The morphological changes in reproductive cells of male mice were observed with light microscope after routine preparation. The results are summarized as follows : 1. Testis, epididymis, prostate, and body weight were increased in control groups, however in the experimental groups, these were severly decreased. 2. The diameter of seminiferous tubule and the height of germinal epithelium cell were decreased, and more numbers of spermatogonia A, spermatogonia B, spermatocyte, spermatid, and spermatozoa were severely decreased. After 35 days treated with chlorambucil, almost all spermatozoa were lost in seminiferous tubules. 3. During the process of treatment, the frequency of cell debris, vacuolations, piknotic nuclei, and multinucleated giant cells were markedly appeared in the germinal epithelial cells. 4. The severe hyperplasia of Leydig cell was revealed, however, the Sertoli cell was not affected with treatment. These results indicate that the chlorambucil causes ultimate lesions for the production of reproductive cell in all stage of the spermatogenesis.

자기방사능법에 의한 DNA in situ Hybridization방법

장성익,양창헌,김대광,정용욱,최인장,이인환 계명대학교 의과대학 1991 계명의대학술지 Vol.10 No.2

To find out the optimal condition for DNA in situ hybridzation using isotopically labeled DNA probe with the general goal of understanding how localization and organization of N-myc oncogene sequences within the SK-N-BE cell line, present investigation was carried out by ³H-labeled pMF3-SEBI-Nmyc probe in situ hybridization. The results were as follows: Specific activities of 2~5×10??? dpm/㎍ of DNA were gained after separation of the ³H labeled DNA by spindialysis using sepharose CL-6B. The chromosomes and interphase nuclei were hybridization with the labeled probe for 14hr at 41℃ in a hybridization mixture solution: 50% formamide, 2×SSCP(2×SSC, 0.04M NaPO4, pH7.0), 10% dextran sulfate, 100㎍/ml of sonicated salmon sperm DNA and 0.2mg/ml each of bovine serum albumin, Ficoll, and polyvinylphyrrolidone. Autoradiography was performed by using half-strength Sakura NR-M2 emulsion for 7-14 days at 4℃. Silver grains were simultaneously detected on Q-banded chromosomes in the same metaphase. Amplifications of N-myc oncogene were conformed on homogeneous staining regions of chromosome 11 and marker chromosome by statistical analysis. It was impossible with this technique to localize N-myc oncogene within the interphase nucleus of SK-N-BE cell line.

Down氏 증후군의 Dermatoglyphics 上의 특징

張性翼,李仁煥,洪海淑 慶北大學校 醫科大學 1980 慶北醫大誌 Vol.21 No.2

지문 및 장문의 변화와 염색체의 변화를 관찰한 결과를 요약하면 다음과 같다. 1. 염색체는 G-trisamy를 나타내었다. 2. 지문의 형태에서 Ulnar Loop가 증가하였다. 3. Total ridge count는 감소하였다. 4. 장문에 있어서 palmar transverse flexion crease에 Simian type의 빈도가 높았다. Mongolism is the commonest syndrome encountered in patients with Autosomal anomaly. G_21 trisomy, associated with mental retardation. Accurate diagnosis of the nature of the chromosomal anomaly is important in order to he ablc to advise the patients upon the risk of involvement of next children, After Nehemiah, on the other hand, dermatoglyphics has been applied to medical field and We observed somewhat different finger prints or palmar creases in mentally retarded children. The purpose of the present investigation was to find out these variations in dermatoglyphics and chromosomal patterns in patients with Down's syndrome compared to normal developed children. Several results were obtained as follows. 1. The chromosome map showed the trisomy of G group. 2. The total ridge count (TRC) was declined and the frequency of ulnar loop was high in the finger prints of these patients. 3. The symian type was frequently appeared in palmar crease.

慶北高靈 44號, 45號 古墳에서 出土된 人骨에 관한 硏究

朱 剛,李仁煥 慶北大學校 醫科大學 1980 慶北醫大誌 Vol.21 No.2

著者들은 慶北 高靈郡 高靈面 池山洞 44號, 45號, 古境에서 出土된 人骨 22例(남자 11, 여자 11)를 調査하여 다음과 같은 結果를 얻었다. 1. 頭長高指數에서 女子는 中頭型이었다. (Orthokran) 2. 頭長耳―Bragma高指數에서 女子는 低頭型이고(chamaekran), 頭幅耳―Bragma高 指數에서 男子는 低頭型(Tapeinokran)이었다. 3. 顔面指數에서 男子는 過扁平鼻(Hyper-cham-aeprosop)이고 上面指數에서 男子는 扁平顔(Cham-aeprosop)이었다. 4. 身長은 男子가 158.9㎝이고, 女子가 157.6㎝이었다, Authors have studied 22 cases of human skeleton excavated from ancient tombs. They are located in Gysan-dong, Koreang-gun of Gyungpook Province. The results are as follows. 1. The cranial index shows orthocranic in female. 2. The length-ear bregma height index is chamaecranic in female, the breadth-ear bregma height index is Tapeinocranic in male. 3. The total facial index is hyperchamaeprosop and the upper facial index is chamaeprosop in male. 4. The height is supposed to be 158.9㎝ in males and 157.6㎝ in females.

자궁경부암에 있어서 특이적 염색체변화와 flow cytometry에 의한 핵산 함량분석

이인환,최인장,정용욱,장성익,김대광 啓明大學校 醫科大學 1990 계명의대학술지 Vol.9 No.2

In order to understand the specific chromosomal changes and CNA content analysis by flow cytometry in cervical cancer of Korean women, firstly we investigated cervical cancer cell line. The chromosomal number of cervical cancer cell line was hyperdipolid and prominent numerical abnormalities were in trisomy 1, 2, 6, 12, 16, 17, 18, 20, 21, 22 and tetrasomy 2. Specific Structural rearrangements were diletion(on1p22, 2p21, 3p21, 3q11, 6q23, 7p14, 7p21, 10p11, 22qll), translocation(3q11, 10p11), isochromosome (5q) and marker chromosome. Common break points at bands 1p22, 2p21, 3p21, 6q23, 22q11 give rise to the loss of cellular oncogene fgr src-2, N-myc, rafl, myb, sis respectively.

만성 골수성 백혈병(CML)에서 염색체의 변화

이인환,최인장,장성익 啓明大學校 醫科大學 1987 계명의대학술지 Vol.6 No.1

This article documents the cytogenetic findings in 43 patients with chronic myelocytic leukemia (CML). Short term culture (3hr) of bone marrow of 26 males and 17 females were studied with different banding techniques. 8 cases were studied during blastic phase (BP), and the others were during chronic phase (CP). typical Philadelpia (PH¹) chromosomes [t(9 : 22) q34 : q11)] were present in 20 cases (47%). Variant was t(14:22) (q32 : q11), additional findings were+8, -22q, -22, i(17q), hyperdiploidy. In BP, 2 cases were hyperdiploid, 2 cases were mosaic pattern, 2 cases had Ph¹ and additional findings (complex translocation), the others had typical Ph¹ alone. Authers discussed the relevance of chromosome change and break point. Certain chromosome regions are more often affected. These might contain genes of critical importance for the final malignant progression. Molecular biology may provide insight on the nature and expression of involved genes.

5-bromodeoxyuridine으로 치환된 CHO세포에 유산암모니움에 의해 제한효소 AluI으로유발된 염색체 이상과 자매염색분체 교환빈도의 증가

최인장,이인환,장성익 대한해부학회 1991 Anatomy & Cell Biology Vol.24 No.1

Human Herpes Virus 6(HHV-6)의 변형유전자의 특성과 위치

최동호,이인환,김홍태,최인장,장성익 啓明大學校 醫科大學 1994 계명의대학술지 Vol.13 No.2

HIV-1 virus와 동반적 감염을 일으키고 AIDS의 진행에 관여하며 다른 AIDS와 관련된 종양형성에 관여할 것으로 의심되는 HHV-6 변형유전자의 위치 및 특성을 알기위하여 변형유전자를 포함한다고 의심되는 DNA분절(Sal 1-L)을 Xbal, BamH1, Hind Ⅲ, Nhel, BstX1의 효소를 절단하여 6가지의 subclone을 얻고 이것을 NIH3T3 세포, CV-1 세포, HUVE 세포에 도입하여 focal assay, anchorage independency test, nude mice에서 tumorigenicity를 조사하고 HIV-LTR과의 상호관계를 조사한 결과를 요약하면 HHV-6의 Sal 1-L의 subclone인 Sal 1-L-SH(1.0kb)를 NIH 3T3, CV-1 및 HUVE에 transfrection하였을 경우 NIH 3T3 및 CV-1 세포배양에서 focus 형성을 보았으며 이것을 cylinder를 이용한 focus cloning을 하고 hygromycin으로 plasmid DNA가 transfection만 된 세포들을 모아(selection) soft agar assay 및 cell aggregation으로 anchorage independency를 확인하였다. Nude mice에 접종하여 종양 형성을 관찰하였으며 이 종양 세포에서 DNA를 추출하여 Southern blot hybridization으로 insert DNA(Sal 1-L-SH)에 기인한 종양임을 알았다. 이로써 HHV-6의 Sal 1-L-SH fragment내에 변형유전자가 포함되어 있음이 확인되었다. 그러나 사람의 혈관내피세포(HUVE)에서는 focus등의 형태학적 변화가 보이지 않았으며 또 HIV-TAT 및 HIV-LTR 등과의 복합적 세포내 도입에서도 세포변형은 얻을수가 없어 Kaposi씨 육종이 혈관 내피세포에서 기인하지 않거나 이 종양셩성에 HHV-6 외의 다른 인자가 관여할 것으로 생각된다. 한편 HIV-LTR CAT과 Sal 1-L-SH 유전자를 같이 transfection하여 CAT assay를 하였을 경우 HIV-LTR의 표현 즉 acetylation이 15배나 항진되어 HHV-6의 Sal 1-L-SH fragment AIDS의 진행을 transactivation으로 촉진시킬 것으로 짐작된다. 더욱이 DNA sequence에 의해 이 유전자가 3개의 ORF를 가지고 있으며 이중 ORF-1에 해당하는 283aa의 polypeptide가 transactivation을 유발시키는 중심단백질로 추정된다. 이에 따라 이 단백질에 대한 항체로 종양의 HHV-6 관련유무를 판단할 수 있을것으로 생각되며 이 단백질을 직접 세포내 도입하여 세포의 변형 등을 추구하여야 할 것으로 생각된다. HHV-6 was isolated in 1986 from patients suffering from lymphoproliferative disorders and AIDS. The antibodies directed against HHV-6 indicate a previous viral infection in 80~90% of the general population. HHV-6 isolates have been divided into two distinct subgroups(subgroup A and B) based on restriction enzyme profiles, prevalence in the population, and their in vitro ability to grow in cultured cells. Subgroup A strain(strains GS and U1102) is correlated with lymphoproliferative disorders. HHV-6 has also been associated with human several malignanicies. The association between HHV-6 and human cancer might be strengthened if other fragments representing different regions of the genome with demonstrated transforming potential were also used for these analyses. These associations have led to the analysis of genomic HHV-6 DNA and cloned restriction fragments(Sal 1-L-SB, -SH, -BN, -BS, -HS, -NS) for their ability to transform cells in vitro. A 4.1-kb Sall-L fragment which exhibited transforming activity was identified. This fragment was retained in focal and tumor-derived cell lines. In addition, the Sal 1-L fragment was shown to transactivate the HIV-1 LTR. Initial experiments have co-localized both the transforming and transactivation activities to a 1.9kb Sall-L-SH fragment and implicate a 283-aa ORF as the functional element. Moreover, the identification of a transforming and transactivation region in HHV-6 has strengthened the association of HHV-6 as a co-factor in AIDS and AIDS-related malignancies.