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Sister Chromatid Exchange(SCE)의 意義
張性翼,朱剛 慶北大學校 醫科大學 1982 慶北醫大誌 Vol.23 No.1
사람의 임파구를 배양하여 BudR 10㎍/㎖와 Hoechst 33258 50㎍/㎖로 처리하여 Giemsa 염색한 결과 전염색체에서 SCE가 나타났다. Sister chromatid exchange (SCE) events which previously have been detected by autoradiography can now be detected by BudR with Hoechst 33258 technique. SCE has been proved to the highly sensitive index of the interaction of carginogens with chromosomes, and abnormalities related to SCE formation occurred in a number of hereditary diseases known or suspected to involve a defect in DNA repair. Blood leukocytes were cultured according to conventional methods. BudR (10㎍/㎖) was added to culture medium 3 hours before colchicine treatment. The lymphocytes incorporated with BudR were stained in Hoechst 33258 and followed Giemsa stain. The result obtained is that SCE seems to be found out on all the chromosomal numbers including sex chromosome.
장성익 啓明大學校 醫科大學 1991 계명의대학술지 Vol.10 No.1
Now, cancer is a disease of gene which can be classified to somatic cell genetic diseases. Cancer might be resulted from a relative genetic insufficiency to tolerate carcinogen exposure in major oncoderm. The balance between exogenous stimulus(carcinogen exposure) and constitutional responce(capacity to resist or repair mutational damage) is altered in favor of the DNA- damaging tendency, and cancer develops. It has been found that the cancer-relevant genes were located on the specific regions of chromosomes. Specific chromosome abnormalities were discovered in leukemia and malignant lymphoma. However, most of solid tumours were not still investigated in specific karyotypes for the specific cancers. Oncogenes are corelated to sites of translocations on the chromosome and antioncogenes are corelated to locus of deletions on the chromosome in cancer cells. Aneuploidy is a phenomenon of cancer progressing which is revealed on the secondary change of mitosis. Recently multistep oncogenesis theory which is concerned with oncogenes, antioncogenes and genomes of the chromosomes has been attractive point of view in explanstion of cancer developing. To understand this hypothesis investigators should approach to cytogenetics. Cancer cytogenetics, even still obscure, may be applied to clinical diagnosis or prediction of prognosis from the patient. I believe that analysis of karyotypes can predict the cancer susceptability from normal state of individuals in the future.
장성익 계명대학교 의과대학 1991 계명의대학술지 Vol.10 No.2
Cancer may be a disease of genes, arising from genetic damage of diverse sorts-recessive and dominant mutations, large rearrangement of DNA and gene translocation on chromosomes, all leading to distorsions of either the expression or biochemical function of genes. The search for these genetic damage in neoplastic cells now is the most important in cancer research. The oncogenes, appear to act dominantly, may directly induce neoplastic growth in experimental animals or malignant transformation in appropriately selected cultured cells. The antioncogenes are recessive at the level of individual cells operating through gene inactivation in tumorigenesis. The carcinogenic process have multistep involving in oncogene and antioncogene at least in the solid cancers. Cancer genes may have roles in regulating differentiation or in the maintenance of fully differentiated cells in normal cells. It is needed now to understand the roles of cancer genes in embryogenesis. Because some genes between relevant to teratology and relevent to oncology have quite genes to clinical tools for early diagnosis, prognosis and therapy.
Down氏 증후군의 Dermatoglyphics 上의 특징
張性翼,李仁煥,洪海淑 慶北大學校 醫科大學 1980 慶北醫大誌 Vol.21 No.2
지문 및 장문의 변화와 염색체의 변화를 관찰한 결과를 요약하면 다음과 같다. 1. 염색체는 G-trisamy를 나타내었다. 2. 지문의 형태에서 Ulnar Loop가 증가하였다. 3. Total ridge count는 감소하였다. 4. 장문에 있어서 palmar transverse flexion crease에 Simian type의 빈도가 높았다. Mongolism is the commonest syndrome encountered in patients with Autosomal anomaly. G_21 trisomy, associated with mental retardation. Accurate diagnosis of the nature of the chromosomal anomaly is important in order to he ablc to advise the patients upon the risk of involvement of next children, After Nehemiah, on the other hand, dermatoglyphics has been applied to medical field and We observed somewhat different finger prints or palmar creases in mentally retarded children. The purpose of the present investigation was to find out these variations in dermatoglyphics and chromosomal patterns in patients with Down's syndrome compared to normal developed children. Several results were obtained as follows. 1. The chromosome map showed the trisomy of G group. 2. The total ridge count (TRC) was declined and the frequency of ulnar loop was high in the finger prints of these patients. 3. The symian type was frequently appeared in palmar crease.