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하현정 ( Hyun Jung Ha ),김순희 ( Soon Hee Kim ),윤선중 ( Sun Jung Yoon ),고연경 ( Youn Kyung Ko ),이은경 ( Eun Kyung Lee ),손영숙 ( Young Sook Son ),김문석 ( Moon Suk Kim ),이종문 ( John M. Rhee ),강길선 ( Gil Son Khang ),이해방 ( 한국조직공학과 재생의학회 2006 조직공학과 재생의학 Vol.3 No.4
In order to fabricate tissue engineered biodisc, cells isolated from nucleus pulposus(NP) and annulus fibrosus(AF) have been characterized with sequential passages. NP and AF cells were separately by enzymatical digestion with 0.25wt% collagenase, respectively. Morphological changes were observed by phase contrast microscope and cell proliferation was counted by hemacytometer. To analyze the biosynthesis of glycosaminoglycan and gene expression of Type I and Type II collagen, safranin-O staining and RT-PCR have been carried out, respectively. Proliferation of NP and AF cells increased up to passage 3 and passage 6. Morphology of NP cells was maintained to passage 5 and it of AF cells was maintained to passage 4. In the result of safranin-O staining, NP and AF cells was stained positively. Type II collagen gene of NP cells was not expressed after passage 6 and expression of Type I collagen gene of AF cells was maintained up to passage 9. It can be expected that these results provide the important information for the application of tissue engineered biodisc.
Enhanced vanillin production from Escherichia coli mutant with improved resistance vanillin toxicity
Eun-Kyung Lee(이은경),Sang-Hawl Yoon(윤상활),Chi Li,Sook-Hee Lee(이숙희),Ju-Eun Kim(김주은),Hye-Min Park(박혜민),Jung-Hun Kim(김정헌),Myng-Suk Choi(최명석),Jae-Kyng Yang(양재경),Won-Taek Seo(서원택),Seon-Won Kim(김선원) 한국생물공학회 2005 한국생물공학회 학술대회 Vol.2005 No.10
Lycopene production in recombinant E. coli engineered with foreign mevalonate pathway
Ju-Eun Kim(김주은),Sang-Hawl Yoon(윤상활),Sook-Hee Lee(이숙희),Hye-Min Park(박혜민),Eun-Kyeong Lee(이은경),Jung-Hun Kim(김정헌),Hossein Shahbarri,Si-Hyoung Lee(이시형),Yong-Chul Shin(신용철),Seon-Won Kim(김선원) 한국생물공학회 2005 한국생물공학회 학술대회 Vol.2005 No.10