한국인 폐경여성에서 골량과 Osteoprotegerin 유전자 다형성 연구

이석우 ( Lee Seog U ),권동진 ( Kwon Dong Jin ),유영옥 ( Yu Yeong Og ),김대훈 ( Kim Dae Hun ),이희중 ( Lee Hui Jung ),김재훈 ( Kim Jae Hun ),김은중 ( Kim Eun Jung ),김진홍 ( Kim Jin Hong ) 대한산부인과학회 2004 Obstetrics & Gynecology Science Vol.47 No.5

골밀도가 감소하는 질환인 골다공증의 발생에는 여러 가지 다양한 요인들이 관여하는 것으로 지금까지 알려져 왔다. 특히 폐경기 여성에서 골다공증의 발생에는 파골세포에 의한 골흡수가 증가하여 발생하는데 Osteoprotegerin (OPG) 유전자가 중요한 역할을 한다. 이에 저자들은 가톨릭대학교 의과대학 성빈센트병원 산부인과 폐경기 크리닉을 방문한 229명의 자연 폐경여성을 대상으로 요추, 대퇴골경부, Ward 삼각주, 대퇴돌기의 골밀도를 측정하여 정상군, 골감소증군과 골다공증군으로 분류하고 골다공증 발생에 있어서 중요한 역할을 하는 것으로 알려진 OPG 유전자의 A^163G, T^950C, G^1181C 다형성을 PCR-RFLP 방법을 이용하여 관찰하고, 이러한 유전자형의 다형성이 골밀도와 어떤 연관성이 있는가를 알아보고자 본 연구를 시도하였다. 저자들의 연구결과에서 대상 폐경기 여성의 A^163G, T^950C, G^1181C 유전자형 분포 양상은 AA 54.6%, AG37.1%, GG8.3%; TT 17.5%, TC 44.1%, CC 38.4%; GG52.4%, GC 38.0%, CC 9.6%를 보였고, 전체 폐경여성에서 A^163G은 요추 부위, G^1181C는 대퇴돌기에서 골밀도와 유의한 상관 관계를 보였다. A^163G 유전자형의 다형성은 요추 부위에서는 정상군과 골다공증군, 대퇴골경부와 Ward 삼각주에서는 정상군에서 골밀도와 유의한 상관 관계를 보였다. T^950C 유전자형의 대퇴골경부 부위에서 정상군과 골감소증군, Ward 삼각주 부위에서 정상군에서만 골밀도와 유의한 상관 관계를 보였다. G^1181C 유전자형의 다형성은 대퇴골경부에서는 골감소증군과 골다공증군, Ward 삼각주와 대퇴돌기에서 골다공증군에서 골밀도와 유의한 상관 관계를 보였다. 이상의 결과로 한국인 폐경여성에서 OPG 유전자의 다형성은 골밀도 변화에 중요한 역할을 하는 것을 알 수 있었다. Objective : To examine the relationship between Osteoprotegerin gene polymorphisms, and bone mineral density (BMD). Methods : Restriction fragment length polymorphisms at the Osteoprotegerin A^163G, T^950C, G^1181C gene site, and BMD at the lumber spine and proximal femur were analyzed in 229 postmenopausal Korean women (81 normal, 111 osteopenic and 37 osteoporotic patients). BMDs were measure by DEXA. The subjects were divided in normal, osteopenic and osteoporotic on the basis of the T-score values according to the classification of the World Health Organization (WHO). Results : The genotype distribution of A^163G, T^950C and G1181C polymorphisms in all postmenopausal women was as follows: AA 54.6%, AG 37.1%, CG 8.3%, T/T 17.5%, T/C 44.1%, C/C 38.4%; CG 52.4%, GC 38.0%, CC 9.6%, respectively. Significant differences in the distribution of A/A and A/G genotype among osteoporotic group were observed. No significant differences in the distribution of T^950C and G^1181C genotypes among three groups were observed. After adjusting for potential confounding factors such as age, BMI, and menopause duration, A^163G polymorphism was significantly associated with BMD at the lumber spine in normal and osteoporotic patients and BMD at the femur neck and wards triangle in normal patients, and G^1181C polymorphism BMD at the trochanter in all groups and BMD at th femur neck in osteopenic and osteoporotic patients, and BMD at the wards triangle and trochanter in osteoporotic patients. But There was no relationship between T^950C gene polymorphism, and BMD. Conclusion : These findings suggest that osteoprotegerin gene polymorphisms may be an important contributor to the variation of BMD among postmenopausal Korean women.

전나무 화분의 발생과정과 활력도 검정

이석우 ( Lee Seog U ),한상돈 ( Han Sang Don ),홍용표 ( Hong Yong Pyo ),( Andrej Kormutak ) 한국산림과학회 2003 한국산림과학회지 Vol.92 No.5

The first data have been presented concerning pollen development and viability in Abies holophylla. Under climatic conditions of the Korea Forest Research Institute the process of microsporogenesis extends from the Mid-February till April 19. Frequencies of meiotic irregularities averaged at 9.1% in two study trees as compared with 16.1% of abortive tetrads, 17.1% of abnormal pollen grains and 12.6% of disturbances during prothalial cell divisions. Cytologically, the most common types of meiotic irregularities were non-incorporated chromosomes into metaphase plates together with anaphase bridges and asynchronous movement of the chromosomes towards the poles of a microsporocyte. Pollen viability parameters in five study trees were mean of 63.39% of germination capacity and 695.62 ㎛ long pollen tubes. Climatically conditioned chromosome damages during prolonged prophase I are supposed to be the cause of a relatively low fertility of A. holophylla pollen.

오존 노출에 의한 층층나무의 유전특성 변화

장석성 ( Jang Seog Seong ),이석우 ( Lee Seog U ),이재천 ( Lee Jae Cheon ),한심희 ( Han Sim Hui ),김홍은 ( Kim Hong Eun ) 한국농림기상학회 2003 한국농림기상학회지 Vol.5 No.4

N/A To examine the effects of ozone (0₃), one of the major air pollutants in the city area, on genetic changes in Corpus controversa Hemsl., we compared genetic structures between sensitive (S) and tolerant (T) tree groups of C. controversa fumigated with ozone using isozyme markers. The genetic structures were measured in terms of allele and genotype frequencies determined at five polymorphic enzyme loci. Marked genetic differences between the two groups were detected at three loci (Lap-2, Mdh-1 and Skdh-1). Genetic parameters, genetic multiplicity, genetic diversity and heterozygosity showed that the tolerant group retained greater genetic variation than did the sensitive group. Results of the study were congruent with the general expectation that the more heterozygous individuals and/ or populations exhibit higher resistance to various stress factors.

낙엽송 임분에 침입한 층층나무의 공간분포에 따른 유전변이

장석성 ( Jang Seog Seong ),홍경락 ( Hong Gyeong Lag ),이석우 ( Lee Seog U ),김홍은 ( Kim Hong Eun ) 한국산림과학회 2004 한국산림과학회지 Vol.93 No.1

This study was conducted to investigate the spatial genetic structure of Cornus controversa growing in a 33-year-old L a n i kaempferi stand in Mt. Odae, Korea. Size of the study plot was 1,500㎡ (30m×50m). The number of trees, averages of tree height and diameter in breast height (DBH) of L . kaempferi which occupied the upper crown layer were 209, 15.7m and 16.2㎝, respectively. C. controvema formed the middle crown layer, and the number of trees, averages of tree height and DBH were 229, 5. ml and 3.7cm, respectively. Species in the study plot were the typical ones growing on the northern slope with less intensity of radiation and good soil humidity. The species composition was similar to that of DryopterZS crassirhizom- C. controvema community group in Mt. Odea. The spatial distribution pattern of L. kaemflfen` was the regularity, but the randomness in C. controvem. The genetic analysis with 229 C. controvem trees for 12 loci in 8 enzyme systems resulted in 4 polymorphic (Lap2 et al.) and 4 monomorphic (Aat-1 et al.) loci. The number of genotypes for 10 alleles in 4 ploymorphic loci and the number of trees per genotype were 33 and 6.9 (SD=12.2), respectively. The maximum number of trees per genotype was 60, and the number of cases that were only one tree per genotype was 11. Spatial autocorrelation analysis using the distance interval of 4m showed that Moran`s I statistic was decreased gradually with the increase of spatial distance until 16m and there was no genetic relationship among trees at the range of 16-24m. The genetic patchy size of C. controvema was 16m. The main factor in the settlement of C. controvem trees in L. kaempfen` stand might be the evacuation of birds eating the seed, instead of seed movement caused by the gravity. If the foraging guild of birds has been regularly active in L . kaem$feri stand, the genetic patchiness of C. controvema can be established without the nearby adults. When trees are selected with the distance interval of 28m, they will be more genetically diverse for the ex situ conservation. Also, it takes small genetic diversity of this study area into consideration, it may be better to collect trees in other regions of Mt. Odae for the effective ex situ conservation.

Alum 슬러지를 이용한 AlPO₄-계 다공성 물질의 합성

강광철,김용호,김진만,이철호,이석우,Kang, Kwang Cheol,Kim, Young Ho,Kim, Jin-man,Lee, Choul Ho,Rhee, Seog Woo 한국공업화학회 2011 공업화학 Vol.22 No.2

본 연구에서는 정수장의 alum 슬러지로부터 $AlPO_4$-계 다공성 물질의 합성과정을 규명하고자 가정용 세제, 휴믹산, 아미노산 등과 같은 유기물질을 틀로 사용하여 $Al(OH)_3$과 인산으로부터 $AlPO_4$-계 다공성 물질을 합성하였으며, $600^{\circ}C$의 공기 중에서 소성을 통하여 틀로 사용한 유기물질을 제거하였다. X-선 회절 분석 결과 합성된 물질은 $AlPO_4$-계 다공성 물질의 특징적인 패턴을 나타내었으며, 물질의 형태적인 특성은 주사전자현미경을 이용하여 관찰하였다. $^{27}Al$ MAS NMR 분석을 통하여 $Al^{3+}$ 이온 주변의 화학적 배위환경의 변화를 관찰하였다. 소성 전 물질에는 4배위와 6배위된 $Al^{3+}$ 이온이 함께 존재하지만, 소성 후 물질에서 $Al^{3+}$ 이온은 모두 4배위 환경에 존재하였다. 합성된 고체 내부에 형성된 기공은 BET 비표면적 측정으로 확인하였다. 최종적으로 합성된 물질의 응용으로 공기 중 유해 포름알데히드 제거 실험을 실시하였으며, 포름알데히드 분자가 물질에 존재하는 기공 표면에 흡착됨을 확인하였다. 결론적으로, alum 슬러지로부터 얻어진 $AlPO_4$-계 다공성 재료를 유해 기체의 흡착 및 제거에 활용할 수 있는 가능성을 제시하였다. In this study, the formation of $AlPO_4$-type porous materials from alum sludge was investigated. The materials were synthesized by the reaction of aluminum hydroxide and phosphoric acid with an organic template. Cationic surfactant, natural humic acid, and amino acids were used for the organic template. The residual organic templates were removed by calcination at $600^{\circ}C$ in the air. Powder X-ray diffraction patterns showed the charicteristic patterns of the $AlPO_4$-type porous materials. The morphology of the material was examined using a scanning electron microscopy. The coordination environment of $Al^{3+}$ ion was investigated by $^{27}Al$ MAS NMR technique. Both tetrahedrally and octahedrally coordinated$Al^{3+}$ ions were found in the as-synthesized samples while all $Al^{3+}$ ions were tetrahedrally coordinated in the calcined products. The development of mesopore in the solid material was confirmed by the measurement of BET specific surface area. Finally, they were used for removal of toxic formaldehyde from the air and the formaldehyde molecules were adsorbed on the surface of pores. In conclusion, $AlPO_4$-type porous materials from alum sludge might be applicable in the removal of toxic volatile organic compounds from the air.

소면적 개발지의 소나무림에서 유전변이의 공간적 분포

홍경락 ( Hong Gyeong Lag ),이석우 ( Lee Seog U ),정재민 ( Jeong Jae Min ),장경환 ( Jang Gyeong Hwan ),강범룡 ( Kang Beom Lyong ) 한국산림과학회 2004 한국산림과학회지 Vol.93 No.1

The objectives of this study were conducted to investigate the spatial genetic structure of a samll stand of Pinus delzsifloru in Anmyon island, which regenerated naturally after clearcut, and to figure out how silvicultural activities affect the genetic structure, About 50% of trees in the study area were thinned out two years ago. Forest age was estimated 27-year-old from tree cores of residuals and tree rings of stumps. Total area of the study site was about 5,000㎡. In September 2001, all 203 trees were mapped and bud samples for isozyme analysis collected within a 50m × 50m area. Tree heights ranged from 10m to 12m and the diameter of breast height averaged over 203 trees was 12.lcm (SD=0.3). Nine polymorphic isozyme loci in 6 isozymes were examined. Proportion of polymorphic loci at 95% level, average number of allele per locus, expected and observed heterozygosities were 77.8%, 2.1, 0.130, and 0.155, respectively. These values were lower than those from the study of 25 P. densiiflora natural populations. On the contrary, the fixation index of 0.161 was much higher than that of 25 natural populations (F_(1s)=-0.008). The individual trees in the study plot appeared to be closely related due to the lack of mother trees that disseminate seeds in the stand. This might be why the fixation index for the study plot was high. Results from spatial autocorrelation analysis with Moran`s index showed that trees within a diameter of 3m were genetically homogeneous. Such a small genetic patchy size observed in the present study might be caused by the differences in microenvironmental selection effects and random mortality of seeds rather than by the seed dispersal patterns. A thinning method similar to the systematic cluster sampling method was performed within the study site. So, it was likely that the thinning had a small effect on the determination of genetic patchy size in the study site. However, we could not trace the change of genetic structure and genetic patchy size before and after thinning because we did not have genetic information for the study area before thinning.

인두유종 바이러스와 관련한 클라미디아 트라코마티스 감염에 대한 임상적 고찰

서경아 ( Seo Gyeong A ),이희중 ( Lee Hui Jung ),이석우 ( Lee Seog U ),윤철구 ( Yun Cheol Gu ),김재훈 ( Kim Jae Hun ),박동춘 ( Park Dong Chun ),권동진 ( Kwon Dong Jin ),박태철 ( Park Tae Cheol ),유영옥 ( Yu Yeong Og ),김대훈 ( Kim 대한산부인과학회 2004 Obstetrics & Gynecology Science Vol.47 No.4

목적: 클라미디아는 하부 생식기 감염의 가장 흔한 원인균의 하나로서 클라미디아 감염 시 증상이 없거나 경미한 예가 많으며 감염 후 난관 폐색, 불임증, 난관 임신, 재발성 골반내 감염 등의 합병증으로 인하여 임상적 중요성이 매우 크다. 이에 클라미디아 감염의 이환율을 조사하고 클라미디아 감염과 연관성 있는 임상적 특성과 인두유종 바이러스와의 유병률을 분석하였다. 연구 방법: 1999년 1월부터 2003년 7월까지 가톨릭대학교 성빈센트병원 산부인과 외래와 Objective: One of the most common causative microorganisms in pelvic inflammatory disease (PID) is the chlamydia trachomatis. In many cases chlamydia trachomatis infection has weak and nearly absent symptom, but it`s endocervical infection usually dissemi

Alum 슬러지를 이용한 AlPO4-계 다공성 물질의 합성

강광철 ( Kwang Cheol Kang ),김용호 ( Young Ho Kim ),김진만 ( Jin Man Kim ),이철호 ( Choul Ho Lee ),이석우 ( Seog Woo Rhee ) 한국공업화학회 2011 공업화학 Vol.22 No.2

본 연구에서는 정수장의 alum 슬러지로부터 AlPO4-계 다공성 물질의 합성과정을 규명하고자 가정용 세제, 휴믹산, 아미노산 등과 같은 유기물질을 틀로 사용하여 Al(OH)3과 인산으로부터 AlPO4-계 다공성 물질을 합성하였으며, 600 ℃의 공기 중에서 소성을 통하여 틀로 사용한 유기물질을 제거하였다. X-선 회절 분석 결과 합성된 물질은 AlPO4-계 다공성 물질의 특징적인 패턴을 나타내었으며, 물질의 형태적인 특성은 주사전자현미경을 이용하여 관찰하였다. 27 Al MAS NMR 분석을 통하여 Al3+ 이온 주변의 화학적 배위환경의 변화를 관찰하였다. 소성 전 물질에는 4배위와 6배위된 Al3+ 이온이 함께 존재하지만, 소성 후 물질에서 Al3+ 이온은 모두 4배위 환경에 존재하였다. 합성된 고체 내부에 형성된 기공은 BET 비표면적 측정으로 확인하였다. 최종적으로 합성된 물질의 응용으로 공기 중 유해 포름알데히드 제거 실험을 실시하였으며, 포름알데히드 분자가 물질에 존재하는 기공 표면에 흡착됨을 확인하였다. 결론적으로, alum 슬러지로부터 얻어진 AlPO4-계 다공성 재료를 유해 기체의 흡착 및 제거에 활용할 수 있는 가능성을 제시하였다. In this study, the formation of AlPO4-type porous materials from alum sludge was investigated. The materials were synthesized by the reaction of aluminum hydroxide and phosphoric acid with an organic template. Cationic surfactant, natural humic acid, and amino acids were used for the organic template. The residual organic templates were removed by calcination at 600 ℃ in the air. Powder X-ray diffraction patterns showed the charicteristic patterns of the AlPO4-type porous materials. The morphology of the material was examined using a scanning electron microscopy. The coordination environment of Al3+ ion was investigated by 27 Al MAS NMR technique. Both tetrahedrally and octahedrally coordinated Al3+ ions were found in the as-synthesized samples while all Al3+ ions were tetrahedrally coordinated in the calcined products. The development of mesopore in the solid material was confirmed by the measurement of BET specific surface area. Finally, they were used for removal of toxic formaldehyde from the air and the formaldehyde molecules were adsorbed on the surface of pores. In conclusion, AlPO4-type porous materials from alum sludge might be applicable in the removal of toxic volatile organic compounds from the air.

자궁경부암 유전자 치료를 위한 p53 종양 억제 유전자를 포함한 아데노 연관 바이러스벡터 (AAVCMVp53)의 제조

김용욱 ( Kim Yong Ug ),서민제 ( Seo Min Je ),배수미 ( Bae Su Mi ),전진근 ( Jeon Jin Geun ),김태형 ( Kim Tae Hyeong ),진태영 ( Jin Tae Yeong ),이석우 ( Lee Seog U ),김용완 ( Kim Yong Wan ),김종국 ( Kim Jong Gug ),이준모 ( Lee Jun M 대한산부인과학회 2004 Obstetrics & Gynecology Science Vol.47 No.4

목적: 재조합 아데노 연관 바이러스 rAAVCMVp53를 생산하기 위해서 3가지 플라스미드 pAAVp53, pAAVLacZ 벡터 플라스미드, packaging 플라스미드, helper 플라스미드를 사용하여 cotransfection 방법을 통해 아데노바이러스 오염이 없는 순수 rAAVCMVp53을 생산하고자 하였다. 연구 방법: 벡터 플라스미드 (pAAVp53, pAAVLacZ), AAV를 암호한 부분에 2개의 p5 promoter를 가지는 플라스미드 Objective: To eliminate the potential problem of adenovirus contamination during recombinant adeno-associated virus (rAAV) vector production, we investigated new rAAV production method by a triple transfection of vector plasmid, packaging plasmid, and ade

국어 속독에 관한 연구 : 그 실태조사 및 훈련의 효과검증을 중심으로

이석우 한국국어교육연구회 1971 국어교육 Vol.- No.17