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- 저자 : 이상섭 ( Sie Won Park (검색결과 3 건)
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박시원,이상섭 ( Sie Won Park,Sang Sup Lee ) 생화학분자생물학회 1976 BMB Reports Vol.9 No.2
Korean BiWhen 3β-acetoxy-5α-chloro-6, 19-oxidoandrostan-l7-one was exposed to the culture broth of Nocardia sp. (ATCC 19170), the compound was hydrolyzed and then dehydrogenated to a 3-keto compound, 6, 19-oxidoandrost-4-ene-3, 17dione. On the other hand, 3 β-acetoxy-5α-bromo-6, 19-oxidoandrostan-l7-one was also hydrolyzed but not further easily oxidized to the corresponding 3-keto compound by the same organism. The discrepancy of this result is considered to be the effect of steroidal 5α-halogen function carrying 6,19-oxido ring on 3β-hydroxysteroid dehydrogenase. In another incubation test, 3 β, 5α-dihydroxy-6β-methylandrostan-l7-one was oxidized to a 3-keto compound much slower rate than dehydroisoandrosterone. In order to clarify the substrate specificity of 5α-halo-6, 19-oxidoandrostane derivatives and 3β, 5α-dihydroxy-6β-methylandrestan-l7-one on 3β-hydroxysteroid dehydrogenase, a cell free enzyme extract was prepared from Rr opaca. This enzyme was induced well with testosterone. It possessed an alkaline optimum pH and required NAD as a cofactor. When reacted with this enzyme, 3β, 5α-dihydroxy-6β-methylandrostan-l7-one and 3β-hydroxy-5 α-chloro-6, 19-oxidoandrostan-l7-one showed relatively retarded turn-over rates to 3-keto compounds as compared with dehydroisoandrosterone. Above all, 3 β-hydroxy-5 α-bromo-6, 19-oxidoandrostan-l7-one showed extremly slow reaction rate to 3-lceto compound. These findings clearly suggest that 5 α-halogen residue of 6,19-oxidosteroids and 5 α-hydroxy residue of 6β-methylsteroid interfere with 3β-hydroxysteroid dehydrogenase activity.
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덱사메사돈이 흰쥐의 뇌의 푸로스타글란딘 $D_2$ 함량에 미치는 영향
박시원,이상섭,Park, Sie-Won,Lee, Sang-Sup 생화학분자생물학회 1984 한국생화학회지 Vol.17 No.3
본 연구는 덱사메사돈이 흰쥐의 뇌의 $PGD_2$(prostaglandin $D_2$)의 기본 준위에 미치는 영향을 관찰하고자 행하였다. 우선 몇가지 스테로이드 호르몬을 식수에 분산 용해 (10 mg/l)시켜 일주일간 경구투여했을 때 전반적으로 전뇌의 $PGD_2$의 함량이 감소되었으며 그 억제 효과는 덱사메사돈의 경우 40%로 가장 현저하였다. 덱사메사돈을 경구투여하여 얻은 시간의존표에 의하면 투여 첫날부터 제 칠일까지 계속 $PGD_2$의 함량 감소 추세가 증가하였다. 덱사메사돈을 복강내 주사로 투여했을 때도 $PGD_2$의 함량 억제가 신속히 일어났으며 투여후 8시간째에 35%의 최고 억제율을 보였다. 아울러 $PGD_2$의 함량 자체가 오후 5시에 최고치(2.4 ng)를 나타내는 일일주기성을 보였다. 용량의존성을 관찰하기 위해서 체중 100 g당 $10\;{\mu}g$에서 $1,000\;{\mu}g$까지 복강내 주사를 행하였으며, $200\;{\mu}g$ 농도까지는 억제효과의 상관성이 나타났다. 이상과 같은 덱사메사돈의 $PGD_2$ 함량에 대한 억제효과가 뇌의 어느 부위에서 가장 현저한지 밝히기 위하여 뇌를 13개 부분으로 나누어 측청한 바 모든 부분에 있어서 비교적 균등하게(18~29%) $PGD_2$의 함량이 감소되었다. 이들 결과로부터 덱사메사돈은 부신-시상하부로 연결되는 부신피질호르몬 분비에 대한 피드백조절자로서의 작용 이외, 전뇌에 고르게 분포되어 그 부분의 세포막에서 유래되는 $PGD_2$의 함량을 감소시킴으로써 중추신경계의 기능에 영향을 미치리라 유추된다. The present study was performed to investigate the effect of dexamethasone on the basal level of $PGD_2$ of rat brain. Primarily, several steroid hormones were administered orally for one week (10 mg/l drinking water) and dexamethasone thereof was shown to have the most strongly decreasing effect (40%) on the level of $PGD_2$ of the whole brain. When dexamethasone was administered orally to find out time dependency, it was demonstrated that there was a steadily increasing abatement of $PGD_2$ in the whole brain from 1st day onward to 7th day of uptake. In a similar way, there was also a fast reduction of $PGD_2$ content with maximum value of 35% by route of intraperitoneal injection. Furthermore, this result of $PGD_2$ decrease by injection showed typical circadian rhythm with peak value (2.4 ng of $PGD_2$/whole brain) at 5:00 PM. In an attempt to observe the dose dependency, this steroid was administered intraperitoneally in concentrations of $10\;{\mu}g$ to $1,000\;{\mu}g$/100g body weight. According to the injected dose, there was a gradual increment of decreasing effect of dexamethasone rather dose dependently to the concentration of $500\;{\mu}g$/100g body weight. As the effect of dexamethasone on the level of $PGD_2$ was very evident, we dissected the brain into 13 parts in order to find out the responsible area for specially reduced content of $PGD_2$ in the brain. However, the obtained data showed that dexamethasone exerts the decreasing effect on the basal level of $PGD_2$ generally with similar ratio (18~29%) in almost all areas. This result implied that dexamethasone may be involved in the physiological homeostasis by feed-back control acting on adrenal-hypothalamus axis as known already and additionally may take part in some other central nervous functions via $PGD_2$ derived from cell membrane by being distributed overally.
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덱사메사돈이 흰쥐의 뇌의 푸로스타글란딘 D2 함량에 미치는 영향
박시원,이상섭 ( Sie Won Park,Sang Sup Lee ) 생화학분자생물학회 1984 BMB Reports Vol.17 No.3
The present study was performed to investigate the effect of dexamethasone on the basal level of PGD₂ of rat brain. Primarily, several steroid hormones were administered orally for one week (10 ㎎/ℓ drinking water) and dexamethasone thereof was shown to have the most strongly decreasing effect (40%) on the level of PGD₂ of the whole brain. When dexamethasone was administered orally to find out time dependency, it was demonstrated that there was a steadily increasing abatement of PGD₂ in the whole brain from 1st day onward to 7th day of uptake. In a similar way, there was also a fast reduction of PGD₂ content with maximum value of 35% by route of intraperitoneal injection. Furthermore, this result of PGD₂ decrease by injection showed typical circadian rhythm with peak value (2.4 ng of PGDZ/whole brain) at 5 : 00 PM. In an attempt to observe the dose dependency, this steroid was administered intraperitoneally in concentrations of 10 ㎍ to 1,000 ㎍/100g body weight. According to the injected dose, there was a gradual increment of decreasing effect of dexamethasone rather dose dependently to the concentration of 500 ㎍/100g body weight. As the effect of dexamethasone on the level of PGD₂ was very evident, we dissected the brain into 13 parts in order to find out the responsible area for specially reduced content of PGD₂ in the brain. However, the obtained data showed that dexamethasone exerts the decreasing effect on the basal level of PGD₂ generally with similar ratio (18∼29%) in almost all areas. This result implied that dexamethasone may be involved in the physiological homeostasis by feed-back control acting on adrenal-hypothalamus axis as known already and additionally may take part in some other central nervous functions via PGD₂ derived from cell membrane by being distributed overally.
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