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제트 혼합 반응기 내 희박 예혼합 메탄-공기 연소의 NO 생성 예측을 위한 화학 반응기 모델링
이보람(Bo-Rahm Lee),박정규(Jung-Kyu Park),이도용(Do-Yong Lee),이민철(Min-Chul Lee),박원식(Won-Shik Park) 대한기계학회 2010 大韓機械學會論文集B Vol.34 No.4
제트 혼합 반응기(JSR) 내의 NOx와 같은 배출물질을 예측하기 위해서 화학반응기 모델을 개발했다. 본 연구에서는 JSR에 대한 화학반응기 모델로서 two-PSR 모델이 채택되었다. CHEMKIN 코드와 4가지 NO 생성 메커니즘을 포함한 GRL 3.0 메탄-공기 연소 메커니즘을 이용해서 JSR내의 희박 예혼합 메탄-공기 연소의 NO 생성예측을 실시하였다. 모델의 검증을 위해서 계산된 결과를 Rutar의 실험 데이터와 비교하였다. NO 생성의 중요파라미터와 4 가지 NO 경로의 기여도를 조사하였다. 화염 영역에서는 prompt 메커니즘이 주된 경로이고 화염후 영역에서는 Zeldovich 메커니즘이 주된 경로이다. 희박 예혼합 조건에서는 N2O 메카니즘이가 화염 및 화염후 영역 모두에서 중요한 경로이다. A chemical reactor model (CRM) was developed for a jet stirred reactor (JSR) to predict the emission of exhaust such as NOx. In this study, a two-PSR model was chosen as the chemical reactor model for the JSR. The predictions of NO formation in lean premixed methane-air combustion in the JSR were carried out by using CHEMKIN and GRI 3.0 methane-air combustion mechanism which include the four NO formation mechanisms. The calculated results were compared with Rutar's experimental data for the validation of the model. The effects of important parameters on NO formation and the contributions of the four NO pathways were investigated. In the flame region, the major pathway is the prompt mechanism, and in the post flame region, the major pathway is the Zelodovich mechanism. Under the lean premixed condition, the N2O mechanism is the important pathway in both flame and postflame regions.
앱타머와 단백질간 가교를 이용한 바이오마커 진단 방법 개발
이보람(Bo-rahm Lee),김진우(Ji-nu Kim),김병기(Byung-Gee Kim) 한국생물공학회 2011 KSBB Journal Vol.26 No.4
The detection of biomarkers is an important issue for disease diagnosis. However, many systems are not suitable to detect the biomarker itself directly. For direct detection of biomarker proteins in human serum, a new affinity-capture method using aptamers combined with the mass spectrometry was suggested. Since signals from protein samples cannot be amplified, modified chromatin immunoprecipitation (ChIP) and subsequent cross-linking with formaldehyde between aptamers and target proteins were used not to lose the captured target proteins, which allowed us to perform a harsh washing step to remove the non-specifically bound proteins. As a model system, a thrombin aptamer was used as a bait and thrombin as a target protein. Using our modified ChIP and affinity-capture method, non-specific binding proteins on the beads decreased significantly, suggesting that our new method is efficient and can be applied to developing diagnosis systems for various biomarkers.
화학 반응기 모델을 이용한 희박 예혼합 메탄-공기 연소의 NOx 생성 경로 연구
이보람(Bo Rahm Lee),박정규(Jung Kyu Park),이민철(Min Chul Lee) 대한기계학회 2009 대한기계학회 춘추학술대회 Vol.2009 No.5
In this study the predictions of NOx in methane-air lean premixed combustion in JSR were carried out with GRI 3.0 methane-air combustion mechanism and Zeldovich, nitrous oxide, prompt, and NNH NO formation mechanism by using CHEMKIN code. The information from CFD modeling for combustor is analyzed and represented as an arrangement of reactor elements. The results are compared to the JSR experimental data of Rutar for the validation of the model. The chemical pathway most likely to form the NO in methane-air lean-premixed combustion was investigated. The results obtained with the 4 different NO mechanisms for residence time(2-4㎳) and pressure(3, 4.7, 6.5 atm) are compared and discussed.
Microbead based micro total analysis system for Hepatitis C detection
심태석(Tae Seok Sim),이보람(Bo-Rahm Lee),이상명(Sang-Myung Lee),김민수(Min-Soo Kim),이윤식(Yoon-Sik Lee),김병기(Byung Gee Kim),김용권(Yong-Kweon Kim) 대한전기학회 2006 대한전기학회 학술대회 논문집 Vol.2006 No.7
This paper describes a micro total analysis system (μ TAS) for detecting and digesting the target protein which includes a bead based temperature controllable microchip and computer based controllers for temperature and valve actuation. We firstly combined the temperature control function with a bead based microchip and realized the on-chip sequential reactions using two kinds of beads. The PEG-grafted bead, on which RNA aptamer was immobilized, was used for capturing and releasing the target protein. The target protein can be chosen by the type of RNA aptamer. In this paper, we used the RNA aptamer of HCV replicase. The trypsin coated bead was used for digesting the released protein prior to the matrix assisted laser desorption ionization time of flight mass spectrometer (MALDI TOF MS). Heat is applied for release of the captured protein binding on the bead, thermal denaturation and trypsin digestion. PDMS microchannel and PDMS micro pneumatic valves were also combined for the small volume liquid handling. The entire procedures for the detection and the digestion of the target protein were successfully carried out on a microchip without any other chemical treatment or off-chip handling using 20μl protein mixture within 20 min. We could acquire six matched peaks (7% sequence coverage) of HCV replicase.